JP7323462B2 - セルフリーｄｎａによる移植患者のモニタリング - Google Patents
セルフリーｄｎａによる移植患者のモニタリング Download PDFInfo
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- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| BR112013020220B1 (pt) | 2011-02-09 | 2020-03-17 | Natera, Inc. | Método para determinar o estado de ploidia de um cromossomo em um feto em gestação |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| EP3134541B1 (en) | 2014-04-21 | 2020-08-19 | Natera, Inc. | Detecting copy number variations (cnv) of chromosomal segments in cancer |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| US11479812B2 (en) | 2015-05-11 | 2022-10-25 | Natera, Inc. | Methods and compositions for determining ploidy |
| RU2760913C2 (ru) | 2016-04-15 | 2021-12-01 | Натера, Инк. | Способы выявления рака легкого |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| CA3067637A1 (en) | 2017-06-20 | 2018-12-27 | The Medical College Of Wisconsin, Inc. | Assessing transplant complication risk with total cell-free dna |
| US12084720B2 (en) | 2017-12-14 | 2024-09-10 | Natera, Inc. | Assessing graft suitability for transplantation |
| US12024738B2 (en) | 2018-04-14 | 2024-07-02 | Natera, Inc. | Methods for cancer detection and monitoring |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| CA3128894A1 (en) * | 2019-02-19 | 2020-08-27 | Sequenom, Inc. | Compositions, methods, and systems to detect hematopoietic stem cell transplantation status |
| WO2020206269A1 (en) * | 2019-04-03 | 2020-10-08 | The Medical College Of Wisconsin, Inc. | Transplant patient monitoring with cell-free dna |
| US11931674B2 (en) | 2019-04-04 | 2024-03-19 | Natera, Inc. | Materials and methods for processing blood samples |
| WO2020247263A1 (en) | 2019-06-06 | 2020-12-10 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| US20230257816A1 (en) * | 2020-07-13 | 2023-08-17 | Aoy Tomita Mitchell | Methods for making treatment management decisions in transplant subjects and assessing transplant risks with threshold values |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011118603A1 (ja) | 2010-03-24 | 2011-09-29 | 凸版印刷株式会社 | 競合プライマーによる標的塩基配列の検出方法 |
| US20150086477A1 (en) | 2012-04-19 | 2015-03-26 | Medical College Of Wisconsin, Inc. | Highly sensitive surveillance using detection of cell free dna |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013509883A (ja) * | 2009-11-06 | 2013-03-21 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | 臓器移植患者における移植片拒絶反応の非侵襲的診断方法 |
| US20130123120A1 (en) * | 2010-05-18 | 2013-05-16 | Natera, Inc. | Highly Multiplex PCR Methods and Compositions |
| KR20160004265A (ko) * | 2013-03-15 | 2016-01-12 | 이뮤코 쥐티아이 다이아그노스틱스, 아이엔씨. | 무세포 dna를 이용하는 신장 상태의 평가를 위한 방법 및 조성물 |
| SI3004388T2 (sl) * | 2013-05-29 | 2023-11-30 | Chronix Biomedical | Odkrivanje in kvantifikacija donorjeve brezcelične DNK v cirkulaciji prejemnikov presajenih organov |
| US20160258010A1 (en) * | 2013-10-18 | 2016-09-08 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Allele specific pcr assay for detection of nucleotide variants |
| HUE043288T2 (hu) * | 2014-03-14 | 2019-08-28 | Caredx Inc | Eljárások immunszuppresszív terápiák monitorozására transzplantátum recipiensben |
| HK1252152A1 (zh) * | 2015-04-30 | 2019-05-17 | Medical College Of Wisconsin, Inc. | 用於評估無細胞dna的多重優化錯配擴增(moma)實時pcr |
-
2018
- 2018-06-20 CA CA3067635A patent/CA3067635A1/en active Pending
- 2018-06-20 US US16/623,725 patent/US20210139983A1/en active Pending
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- 2018-06-20 AU AU2018288838A patent/AU2018288838A1/en not_active Abandoned
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011118603A1 (ja) | 2010-03-24 | 2011-09-29 | 凸版印刷株式会社 | 競合プライマーによる標的塩基配列の検出方法 |
| US20150086477A1 (en) | 2012-04-19 | 2015-03-26 | Medical College Of Wisconsin, Inc. | Highly sensitive surveillance using detection of cell free dna |
Non-Patent Citations (4)
| Title |
|---|
| BMC genomics, 2007, Vol.8, 275 (pp.1-9) |
| Clinical Chemistry，2009年，vol.55, no.11，p.1958-1966 |
| Mutat. Res., 1999, Vol.430, pp.1-12 |
| PLOS Medicine，2017年，vol.14, no.4,e1002286，p.1-19 |
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| JP2020529194A (ja) | 2020-10-08 |
| EP3642356A1 (en) | 2020-04-29 |
| EP3642356A4 (en) | 2021-03-17 |
| AU2018288838A1 (en) | 2020-01-16 |
| WO2018237081A1 (en) | 2018-12-27 |
| IL271459A (en) | 2020-01-30 |
| CN110945144A (zh) | 2020-03-31 |
| CA3067635A1 (en) | 2018-12-27 |
| US20210139983A1 (en) | 2021-05-13 |
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