JP7295165B2 - Method for suppressing the generation of flies in a bee trap - Google Patents

Description

The present invention relates to a bee trapping agent, a bee trap containing the trapping agent and a method of trapping bees using the bee trap. More specifically, it relates to an invention characterized by using a surfactant having a specific penetrating power as an active ingredient.

Conventionally, various types of pest traps have been known. For example, pest attracting and trapping devices for small flying pests such as cockroaches and flies are widely used in general households, restaurants, factories, etc. there is
Also, bee traps of various shapes have been proposed. Specifically, Patent Document 1 describes a catcher in which an attracting and capturing composition for attracting bees is housed inside a cup-shaped container, and a lid having a bee entrance is attached to the top of the container. there is Patent Documents 2 and 3 introduce techniques for improving the capture efficiency of bees by adjusting the visible light transmittance and color of traps. Patent Literature 4 describes a trap having a bee entrance located at the center of the upper part of the trap in order to prevent trapped bees from escaping from the trap. Furthermore, Patent Document 5 describes that by using a bee-attracting component with a specific sugar concentration, a fermented odor due to putrefaction is generated to improve trapping efficiency.

However, when a liquid attractant is used in these bee traps, even if a bee falls into the liquid attractant, it will take some time before the bees die, during which time the bees will be exposed to the liquid attractant. There is a problem that they swim in the trapping agent and escape from the container, which lowers the trapping efficiency. In addition, even if a bee dies, the dead bee floats on the liquid surface of the liquid attractant and trapping agent, and this serves as a foothold for another bee to encourage its escape.

JP 2004-073144 A JP 2007-174964 A JP 2009-247236 A JP 2014-103933 A JP 2007-176853 A

An object of the present invention is to reduce the time it takes for trapped bees to die.

As a result of intensive research to solve the above problems, the present inventors have found that a surfactant having a specific penetrating power not only shortens the time until bees die, but also makes the surfactant effective. It was found that dead bees settled in the bee-trapping agent as a component, and the above problem was solved by using a bee-trapping agent containing a surfactant having a specific penetrating power as an active ingredient. It is.

Specifically, the gist of the present invention is as follows.
1. A method for suppressing the generation of small flies in a bee trap using the following surfactant (A) having a penetration power of 2000 seconds or less.
Surfactant (A) is polyglyceryl laurate (10), polyoxyethylene alkyl (C12-14) ether, sodium lauryl sulfoacetate, sucrose laurate, sucrose myristate, polyoxyethylene tristyryl phenyl Ether, polyglyceryl laurate (5), polyoxyethylene distyrylated phenyl ether, polyglyceryl laurate (10), polyoxyethylene coconut oil fatty acid sorbitan, polyoxyethylene sorbitan monolaurate (20E.O.), polyglyceryl myristate ( 5), polyoxyethylene polyoxypropylene cetyl ether (20E.O.) (8P.O.), polyoxyethylene tribenzylphenyl ether, polyoxyethylene sorbitan monooleate (20E.O.), sucrose oleic acid One or more selected from esters, polyethylene glycol monostearate (40 E.O.).

The bee-trapping agent of the present invention exhibits an excellent bee-trapping effect without the escape of bees from the bee trap because it takes only a short time to kill the bees. Moreover, in the bee-trapping agent of the present invention, the dead bees settle in the liquid of the bee-trapping agent, so that the dead bees float on the liquid surface of the bee-trapping agent, serving as a foothold for other bees to facilitate their escape. This is useful because it does not prevent other bees from coming into contact with the bee-trapping agent, so that bees can be efficiently captured.
In addition, the bee-trapping agent of the present invention can be combined with a known insecticide to make a control agent that can reliably control attracted bees, and is useful because it can easily obtain an efficient control effect against bees. is.

It is a graph which shows the cumulative total of the number of wasps captured in the "test to confirm the effect of capturing the hornet."

Hereinafter, the bee-trapping agent of the present invention, the bee-trapping device containing this trapping agent, and the bee-trapping method using this bee-trapping device will be described in detail.
The bee-trapping agent of the present invention contains a surfactant (A) having specific penetrating power as an active ingredient.
<About penetration power of the present invention>
The penetrating power in the present invention means the time (seconds) measured by a measuring method conforming to the canvas disc method (ISO-8022-1990) described below, and the measurement is performed five times and the average value is the present value. Penetration power (seconds) in the invention.
(Measuring method)
At 20 ° C., a woolen cloth (coated white cloth for dyeing fastness test: JIS L 0803 compliant, hair (wool), size: 200 mm × 200 mm) is fixed in a φ150 mm test piece holding frame without slack, and measured. 50 μL of a 1% by weight aqueous solution of the target surfactant is dropped on the surface of the central portion of the wool fabric to form droplets, and the time until the droplets are absorbed by the wool fabric is measured with a stopwatch.
The endpoint at which the droplet is absorbed by the wool fabric is determined by the disappearance of the specular reflection. Specifically, light of 500 to 1000 lux is applied to the periphery of the droplet to visually confirm the specular reflection of the droplet. As the droplet is gradually absorbed by the wool fabric, the specular reflection of the droplet becomes weaker, and when the droplet is completely absorbed by the wool fabric, the specular reflection of the droplet disappears. This makes it possible to objectively determine the endpoint at which the droplets have been absorbed by the wool fabric. This criterion is a method applied in the water absorption test method for textile products (JIS 1907:2010).
The active ingredient of the bee trapping agent of the present invention is a surfactant having a penetration power of 2000 seconds or less at 20° C. in a 1% by weight aqueous solution, preferably a surfactant having a penetration power of 1000 seconds or less. Surfactants that are sub-seconds are more preferred.

The active ingredient of the bee-trapping agent of the present invention is a nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, as long as it is a surfactant having the penetration power of 2000 seconds or less. Any active agent can be used without particular limitation, but nonionic or anionic surfactants are preferred.
Specifically, for example, nonionic surfactants include sugar ester type, fatty acid ester type, vegetable oil type, alcohol type, alkylphenol type, polyoxyethylene-polyoxypropylene block polymer type, alkylamine type, and bisphenol type surfactants. , and polyaromatic ring types. Examples of anionic surfactants include carboxylic acid type, sulfonic acid type, sulfate type, and phosphate type surfactants. Examples of cationic surfactants include ammonium-type and benzalkonium-type surfactants. Amphoteric surfactants include those of the betaine type.
Among them, preferred nonionic surfactants include polyglycerin fatty acid esters, polyoxyalkylene alkyl ethers, polyoxyalkylene aryl ethers, polyoxyalkylene sorbitan fatty acid esters, polyalkylene glycol fatty acid esters, sucrose fatty acid esters, and the like. Suitable anionic surfactants include sulfonates and the like.
Particularly preferred nonionic surfactants include polyglycerin fatty acid esters, polyoxyethylene alkyl ethers, polyoxyethylene polyoxypropylene alkyl ethers, polyoxyethylene aryl ethers, polyoxyethylene sorbitan fatty acid esters, polyethylene glycol fatty acid esters, Sucrose fatty acid esters are included, and particularly suitable anionic surfactants include alkyl sulfoacetates. The surfactant (A), which is the active ingredient, may be used alone or in combination of two or more.

The bee-trapping agent of the present invention is obtained by simply diluting the surfactant (A) having excellent penetrating power with water, the surfactant (A) having excellent penetrating power and having excellent penetrating power, It includes those formulated with insecticides and ingredients that are generally added to formulations, and further those formulated and diluted with water. Among them, as the bee trapping agent of the present invention, a formulation of the surfactant (A) having excellent penetrating power and other ingredients is compact and has excellent storage stability. It is advantageous during storage. Formulations include liquid formulations such as oils, emulsions, wettable powders, and flowable formulations (suspension in water, emulsion in water, etc.), as well as solid formulations such as microcapsules, powders, granules, and tablets. mentioned. Among them, liquid preparations are preferable because they are easily diluted with water and leave little undissolved residue. As water used for dilution, purified water, tap water, ion-exchanged water, distilled water, filtered water, sterilized water, underground water, well water, and the like are used.
The bee-trapping agent of the present invention preferably contains 0.005% by weight or more of the surfactant (A) having excellent penetrating power, which is an active ingredient, with respect to the entire bee-trapping agent. It is more preferably contained in an amount of 0.01% by weight or more, and particularly preferably in an amount of 0.05% by weight or more. The content of the surfactant (A), which is an active ingredient and has excellent penetrating power, is less than 0.005% by weight with respect to the entire bee-trapping agent. The capture effect cannot be sufficiently exhibited. Moreover, if the content exceeds 5% by weight, problems in formulation such as solubility arise, so the content is preferably 5% by weight or less.

By using the bee-trapping agent of the present invention in combination with a known insecticide, it can be made into a control agent capable of reliably controlling attracted bees. Examples of known insecticides that can be used in combination include natural pyrethrin, allethrin, resmethrin, flamethrin, prallethrin, terarethrin, phthalthrin, phenothrin, permethrin, cyphenothrin, cypermethrin, transfluthrin, metofruthrin, profluthrin, imiprothrin, empentrin, and etofenprox. , silafluofen, meperfluthrin, dimefluthrin and other pyrethroid compounds; propoxur, carbaryl and other carbamate compounds; fenitrothion, DDVP and other organophosphorus compounds; methoxadiazone and other oxadiazole compounds; fipronil and other phenylpyrazole compounds; Neonicotinoid compounds such as imidacloprid and dinotefuran; insect juvenile hormone-like compounds such as pyriproxyfen, methoprene and hydroprene; anti-juvenile hormone-like compounds such as precocene; chitin such as novarron and diflubenzuron Synthetic inhibitor; various insecticides such as essential oils such as phytoncide, peppermint oil, orange oil, cinnamon oil, clove oil, etc. Synergists such as synepyrine and piperonyl butoxide are also used in combination. be able to. Less repellent insect growth regulators such as insect juvenile hormone-like compounds, anti-juvenile hormone-like compounds, chitin synthesis inhibitors, etc. can also be preferably used in combination.

Among these known insecticides, when the bee-trapping agent of the present invention and the insecticide are mixed and used, the water solubility at 20° C. is preferably 100 ppm or more. Examples of water-soluble insecticides include acephate, bamidothion, methidathione (DMTP), fenocarb (BPMC), ethiophenecarb, cartap, thiocyclam, imidacloprid, thiacloprid, cyromazine, fosthiazate, acetamiprid, thiamethoxam, carbaryl (NAC). , clothianidin, pymetrozine, dinotefuran and the like. Among these, for example, dinotefuran (water solubility at 20°C: about 54000 ppm), thiamethoxam (water solubility at 20°C: about 4100 ppm), imidacloprid (water solubility at 20°C: about 510 ppm), fenocarb (BPMC, water at 20°C Solubility: about 610 ppm), those with a water solubility of 500 ppm or more at 20° C. are more suitable as insecticides to be combined with the bee-trapping agent of the present invention.

The bee-trapping agent of the present invention can attract and reliably trap bees when used in combination with known attractants. Known attractants include, for example, balsamic vinegar, apple cider vinegar, rice vinegar, brown rice vinegar, lees vinegar, soybean vinegar, black vinegar, wine vinegar, sudachi vinegar, red vinegar, persimmon vinegar, malt vinegar, purple potato vinegar, and sugar cane. Vinegar and other vinegars, lactic acid products (lactic acid bacteria drinks, etc.), sugars, starch sugars, honey, fruits, processed fruit products, fruit juices, fruit juice drinks, fruit wines, etc., alcoholic beverages, sake lees, seafood, processed seafood products, seafood It may be based on extracts, meat, processed meat products, meat extracts, flavorings, and the like. Among the above attractants, liquid ones are particularly preferred. Furthermore, if moisturizing ingredients such as aliphatic polyhydric alcohols such as glycerin, sugar alcohols such as sorbitol, and thickening polysaccharides such as xanthan gum are included, the effects of the attracting ingredients can be exerted over a long period of time.

The bee-trapping agent of the present invention can be formulated by containing components generally added to formulations within a range that does not interfere with the effects of the present invention. Examples of ingredients commonly added to formulations include stabilizers, preservatives, colorants, anti-ingestion agents, liquid carriers and the like. Examples of stabilizers include antioxidants such as dibutylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), ascorbic acid, and the like. Examples of preservatives include sorbic acid, sorbates, parahydroxybenzoic acid esters, thiabendazole and the like. Examples of coloring agents include caramel pigments, gardenia pigments, anthocyanin pigments, safflower pigments, flavonoid pigments, Red No. 2, Red No. 3, Yellow No. 4 and Yellow No. 5. Examples of accidental ingestion preventive agents include denatonium benzoate and the like.

Examples of liquid carriers used for formulation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, benzyl alcohol, ethylene glycol, etc.), ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, propylene glycol monomethyl ether, tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, ethyl lactate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatics or aliphatic hydrocarbons (xylene, toluene, alkylnaphthalene, phenylxylylethane, kerosene, light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (acetonitrile, isobutyronitrile, etc.), sulfoxides (dimethylsulfoxide, etc.), heterocyclic solvents (sulfolane, γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, 1,3-dimethyl-2-imidazolidinone), acid amides (N,N-dimethylformamide, N,N-dimethylacetamide, etc.), alkylidene carbonates (propylene carbonate, etc.), vegetable oils (soybean oil, cottonseed oil, etc.) , plant essential oils (orange oil, hyssop oil, lemon oil, etc.), and water.

Bees for which the bee-trapping agent of the present invention exhibits an excellent trapping effect are mainly intended for bees belonging to the Vespidae family. Examples include those belonging to the order Pests. Among them, the Waspidae bees include bees belonging to the subfamily Vespinae and Polistinae.
Examples of bees belonging to the subfamily Waspidae include giant hornets, yellow hornets, little hornets, hornets, hornets, brown hornets, black hornets, hornets, and yellow hornets.
In addition, bees belonging to the Polistes subfamily include, for example, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, and paper wasps. Indigenous species may be mentioned. In addition to these indigenous species, there is also the Asian hornet that has invaded Tsushima and Kitakyushu. Although the aggressiveness and toxicity of the paper wasp are less than those of the hornet, they sometimes build nests in private houses, and there have been cases of death due to their stings.
Among these bees, the bee trapping agent of the present invention is a bee with a body length of 10 mm or more and 45 mm or less, preferably a body length of 10 mm or more and 30 mm or less, more preferably a body length of 10 mm or more and 25 mm or less. Demonstrates an excellent trapping effect against bees.

<About the bee catcher>
The bee trap of the present invention may be stored in a container and used as a bee trap.
The shape, size, etc. of the container are not limited as long as it can contain the bee-trapping agent of the present invention. The material of the container is not particularly limited, for example, glass, metal, plastic, etc., as long as it is a material such as special paper having a waterproof or water-repellent function that prevents the bee trapping agent of the present invention from leaking out of the container. .
As one embodiment of the container, it is preferable that the container has a lid covering the opening of the container, and either the lid or the container has an opening through which the bees enter. The size and shape of the opening may be such that bees can easily enter the container. It is more preferable to set the following. If the opening is smaller than 20 mm, it is difficult for large-sized bees such as Asian giant hornets to enter through the opening, and the trapping efficiency is lowered. Moreover, if the opening is larger than 35 mm, butterflies, moths, and beetles other than bees may enter the container. It is preferable to form a plurality of openings in the container so that bees can easily enter the container. preferable. Moreover, in order to prevent bees that have entered the container from easily escaping out of the container, the opening preferably has a shape such as a funnel shape.
The container may have a transparent or translucent window or a transparent or translucent container so that the number of captured bees can be visually observed. can.
When installing the bee trap outdoors, in order to prevent rainwater and the like from entering and diluting the bee trap of the present invention and reducing the efficiency of bee trapping, it is necessary to provide a space for the opening and prevent rainwater and the like from deteriorating. A mode having a cover for preventing infiltration is preferred.
The bee catcher of the present invention is preferably used by hanging it at a height of about 1 to 3 m from the ground, or by fixing it to a fence or the like, avoiding direct sunlight as much as possible.

Hereinafter, the present invention will be described in more detail with formulation examples, test examples, etc., but the present invention is not limited to these examples.
In the examples, parts means parts by weight unless otherwise specified.

<Confirmation test of penetrating power>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a 1% by weight uniform aqueous solution, which was used as a test sample.
(2) Measurement of penetration force At 20 ° C., wool fabric (coated white cloth for dyeing fastness test: JIS L 0803 compliant, hair (wool), size: 200 mm × 200 mm), φ 150 mm stainless petri dish and plastic test piece It was fixed with a holding frame in a tightly stretched state, placed between the light source (935 lux) and an observer, and a Pipetman for dropping the test sample was installed so that the tip and the surface of the wool fabric were 10 mm apart. The observer observed from an angle at which the specular reflection of the water could be reliably confirmed. 50 μL of the test sample was dropped from a Pipetman, and specular reflection of the droplet formed on the surface of the wool fabric was observed. The time (in seconds) from the time the test specimen reaches the surface of the woolen fabric until the woolen fabric has absorbed all of the droplet (disappearance of the specular reflection), leaving only wetness on the surface of the woolen fabric, Measured with a stopwatch. The measurement was performed 5 times, and the average value was taken as the penetrating power (seconds) of the surfactant used.

<Bee lethal effect confirmation test 1>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a uniform aqueous solution of 0.06% by weight, which was used as a test sample of the bee-trapping agent.
(2) Test method Each test sample was poured into a container (caliber 92 mm, height 131 mm, bottom diameter 58 mm, manufactured by Tokan Kogyo Co., Ltd.) from the bottom to a height of 100 mm. One European honey bee (hereinafter referred to as "test bee") having a body length of about 10 to 15 mm was placed on the liquid surface of the test sample, and the time until the action stopped was measured. This test was repeated three times, and the average value was taken as the bee lethal time (seconds).

<Bee drowning effect confirmation test 1>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a uniform aqueous solution of 0.06% by weight, which was used as a test sample of the bee-trapping agent.
(2) Test method Each test sample was poured into the same container as in the above-mentioned "Bee lethal effect confirmation test 1" to a height of 100 mm from the bottom. Seven test bees were placed on the test sample liquid surface, and the number of drowning test bee carcasses after 24 hours was confirmed. Here, drowning means that the carcasses of the test bees have sunk to the bottom of the container, and does not mean those that float below the surface of the water or in the liquid.
The drowning rate (%) was calculated from the following formula (1).
Formula (1): drowning rate (%) = 100 × (test bees that have settled to the bottom of the container) / (test bees number 7)

Penetration power (seconds), bee death time (seconds), and drowning rate (%) of each test sample are shown together. According to the following [Evaluation Criteria] regarding bee lethal time (seconds) and drowning rate (%), the respective evaluation results are also shown in Table 1 below.
[Evaluation criteria for bee lethal time (seconds)]
A: Bee lethal time (seconds) is less than 200 seconds B: Bee lethal time (seconds) is 200 seconds or more and less than 400 seconds C: Bee lethal time (seconds) is 400 seconds or more and less than 600 seconds D: Bee lethal time (seconds) is 600 seconds or longer or escapes. Evaluation criteria A to C for the bee lethal time (seconds) were judged to be practical.
[Evaluation criteria for drowning rate (%)]
A: Drowning rate (%) is 80% or more B: Drowning rate (%) is 65% or more and less than 80% C: Drowning rate (%) is 50% or more and less than 65% D: Drowning rate (%) is less than 50% or escapes ×: Means escape from the container containing the test specimen Evaluation criteria A to C for the drowning rate (%) were judged to be practical.

Details of the surfactants used are as follows.
Surfactant 1: polyglyceryl laurate (10), HLB: 15.5
Surfactant 2: polyoxyethylene alkyl (C12-14) ether, HLB: 13.5
Surfactant 3: sodium lauryl sulfoacetate Surfactant 4: sucrose laurate, HLB: 16
Surfactant 5: sucrose myristate, HLB: 16
Surfactant 6: polyoxyethylene tristyrylated phenyl ether, HLB: 12
Surfactant 7: polyglyceryl laurate (5), HLB: 15.8
Surfactant 8: Polyoxyethylene distyrylated phenyl ether, HLB: 12.8
Surfactant 9: polyglyceryl laurate (10), HLB: 17.1
Surfactant 10: polyoxyethylene coconut oil fatty acid sorbitan, HLB: 16.7
Surfactant 11: polyoxyethylene sorbitan monolaurate (20 E.O.), HLB: 16.7
Surfactant 12: polyglyceryl myristate (5), HLB: 15.4
Surfactant 13: Polyoxyethylene polyoxypropylene cetyl ether (20E.O.) (8P.O.), HLB: 12.5
Surfactant 14: Polyoxyethylene tribenzylphenyl ether, HLB: 13.2
Surfactant 15: polyoxyethylene sorbitan monooleate (20 E.O.), HLB: 15.0
Surfactant 16: sucrose oleate, HLB: 15
Surfactant 17: Polyethylene glycol monostearate (40 E.O.), HLB: 17.5
Surfactant 18: Polyoxyethylene phytostyrol, HLB: 12.5
Surfactant 19: polyoxyethylene phytostyrol, HLB: 18.0
Surfactant 20: polyoxyethylene hydrogenated castor oil, HLB: 12.5
Surfactant 21: polyoxyethylene hydrogenated castor oil, HLB: 14.0

As shown in Table 1, the bee-trapping agents containing surfactants 1 to 17, which are surfactants (A) having a penetrating power of 2000 seconds or less, as active ingredients take a short time to kill bees, Moreover, it was confirmed that the carcasses of the bees were drowned in the liquid of the bee-trapping agent. In particular, it was revealed that the bee-trapping agent containing surfactants 1 to 16 having a penetrating power of 1000 seconds or less of the present invention as an active ingredient exhibits an excellent bee-trapping effect. In the case of surfactants 18 and 19, although bees were put into the test specimen, the bees escaped from the test specimen and did not die. Therefore, "-" in Table 1 means that the time to death and drowning rate could not be measured for the above reason.

<Bee lethal effect confirmation test 2>
(1) Preparation of Test Specimen A bee-attracting component (liquor, vinegar, sugar, etc.) is combined so that the content of the surfactant 1 is 0.3% by weight to prepare a bee-trapping agent undiluted solution. Example test specimens were prepared by diluting the stock solution 5-fold with water. In addition, a comparative example test sample that did not contain Surfactant 1 was prepared in the same manner as the example test sample.
(2) Test method A test was performed in the same manner as in the above "Lethal Bee Effect Confirmation Test 1", and the time until behavior stopped was measured. This test was repeated three times, and the average value was taken as the bee lethal time (seconds). Table 2 shows the results of Example 1 using the Example test sample and Comparative Example 1 using the Comparative Example test sample. The evaluation criteria were also the same as those in the above-mentioned "Bee lethal effect confirmation test 1".

<Bee drowning effect confirmation test 2>
(1) Preparation of Test Specimens Example test specimens and comparative example test specimens of the above-mentioned "Bee Lethal Effect Confirmation Test 2" were used.
(2) About the test method Except for confirming the number of drowning of the test bee carcasses after 84 hours, the test was performed in the same manner as in the above "Bee drowning effect confirmation test 1", and the drowning rate (%) was calculated. bottom. Table 2 shows the results of Example 1 using the Example test sample and Comparative Example 1 using the Comparative Example test sample. The evaluation criteria were also the same as those in the above-mentioned "Bee Drowning Effect Verification Test 1".

As shown in Table 2, in Example 1 in which surfactant 1, which is the surfactant (A) having a penetrating power of 2000 seconds or less of the present invention, and an attractant were mixed, the time until the bees died was short, and it was confirmed that the carcasses of the bees were drowned in the liquid of the bee-trapping agent. On the other hand, in Comparative Example 1, which is an attractant that does not contain Surfactant 1, although the bees drowned, it took a very long time. Furthermore, the bee carcasses hardly drowned in the liquid of Comparative Example 1, and were in a state of floating on the surface of the liquid. For this reason, when Comparative Example 1 is used outdoors, even if bees are attracted, the bees are captured first, and the bees floating on the liquid surface of Comparative Example 1 become a foothold, It is presumed that the bees attracted from the liquid of Comparative Example 1 are more likely to escape without being trapped and drowning.

<Bee drowning/lethal effect confirmation test>
(1) Preparation of Test Specimen Surfactant 1 was mixed with water to obtain a 0.1% by weight uniform aqueous solution, which was used as an example test specimen of the bee-trapping agent.
(2) Test method The test sample was poured into a container (caliber 92 mm, height 131 mm, bottom diameter 58 mm, manufactured by Tokan Kogyo Co., Ltd.) from the bottom to a height of 100 mm. A paper wasp with a body length of about 20 to 25 mm (hereinafter referred to as "test bee") was placed on the liquid surface of the test sample, and the time until drowning was measured. This test was repeated twice, and the average value was taken as the bee lethal time (seconds).
In addition, 24 hours after the drowning, it was confirmed whether the carcasses of the test bees had sunk to the bottom of the container, and the drowning rate (%) was calculated in the same manner as in the above-mentioned "Bee Drowning Effect Verification Test 1".
Example Using the test sample as Example 2, Table 3 summarizes the bee lethal time (seconds), the drowning rate (%), and the evaluation results. The evaluation criteria were also the same as those in the above-mentioned "Bee Drowning Effect Verification Test 1".

As shown in Table 3, Example 2 containing Surfactant 1, which is the surfactant (A) having a penetrating power of 2000 seconds or less of the present invention, has a short time to death even for paper wasps. Moreover, it was confirmed that the carcasses of bees were drowned in the solution of the bee-trapping agent.

<Confirmation test of wasp trapping effect>
In order to confirm the trapping effect of the wasp trapping agent of the present invention on wasps, a wasp trapping effect confirmation test was conducted outdoors. As a reference example, a commercially available trap containing bee attractant (commercial product A) was used.
(1) Preparation of Example Specimen Using the example test specimen of the above "Bee lethal effect confirmation test 2", this is placed in a bee trap that does not contain the commercial product A bee attractant, and the commercial product A and Example 3 was prepared by injecting the same amount of liquid.
(2) About the test method In the forest (test area 1) and in the garden near the building (test area 2), the above Example 3 and the reference example were placed 3 m apart, and installed at a height of 2 m above the ground. Cumulative catches were observed over 4 weeks (May 2018). The test was conducted at the above two locations, and the total was taken as the total number of wasps caught.
Table 4 and FIG. 1 show the total number of wasps captured in Example 3 and Reference Example in Test Areas 1 and 2, and Table 5 shows the types of wasps that were captured.

As shown in Table 4 and FIG. 1, the bee trap containing the bee trap of the present invention (Example 3) was able to capture more bees in the early stages of the first to second week than the commercial product of the reference example. It was confirmed that the effect was very good, and that about 1.7 times as many bees could be captured by the fourth week. This result is considered to be due to the fact that the bee-trapping agent of the present invention contains, as an active ingredient, a surfactant that has excellent penetrating power and takes a short time to kill bees. Moreover, the bee-trapping agent of the present invention (Example 3) has the effect of causing the dead bees to settle in the bee-trapping agent. Therefore, it is thought that the number of bees caught increased steadily from the initial stage of the start of the capture.
When the inside of the bee trap was actually observed, it was found that the bees trapped in the bee trap of the present invention were drowning on the bottom of the trap. Flies were also captured, and their carcasses were all floating on the surface of the captured liquid. As a result, the dead bodies floating on the surface of the trapping liquid covered the surface of the liquid. Since the bee trap of the present invention also sediments the carcasses of other insects such as moths and flies that have been captured, it is possible to obtain an excellent bee trapping effect over a long period of time without interfering with the trapping of new bees and their drowning. can be useful.
Furthermore, the commercially available product of the reference example had the drawback that the fruit flies spawned, and a large number of the fruit flies were generated in the trap, resulting in an unsightly appearance. On the other hand, in the bee trap of the present invention, the captured flies are quickly drowned and the carcasses settle, so that there is no large outbreak of flies due to spawning, and it is clear that the bee trap can be used continuously with a clean appearance. became.
In addition, as shown in Table 5, the bee trap containing the bee trap of the present invention (Example 3) has a larger size than the commercial product of the reference example, the giant hornet (about 25 to 45 mm in body length), the hornet (about 22 mm in body length), ~30 mm) and yellow hornets (approximately 17 to 28 mm), which are highly aggressive carnivorous bees.

The bee-trapping agent of the present invention exhibits an excellent bee-trapping effect without the escape of bees from the bee trap because it takes only a short time to kill the bees. Moreover, in the bee-trapping agent of the present invention, the dead bees settle in the liquid of the bee-trapping agent, so that the dead bees float on the liquid surface of the bee-trapping agent, serving as a foothold for other bees to facilitate their escape. This is useful because it does not prevent other bees from coming into contact with the bee-trapping agent, so that bees can be efficiently captured.
In addition, since the bee-trapping agent of the present invention drowns insects other than the trapped bees and the carcasses settle, for example, there is no large-scale outbreak of small flies, etc., and the bee-trapping agent has an excellent bee-trapping effect over a long period of time, as well as a beautiful appearance. can be maintained.

Claims (1)

A method for suppressing the generation of small flies in a bee trap using the following surfactant (A) having a penetration power of 2000 seconds or less.
Surfactant (A) is polyglyceryl laurate (10), polyoxyethylene alkyl (C12-14) ether, sodium lauryl sulfoacetate, sucrose laurate, sucrose myristate, polyoxyethylene tristyryl phenyl Ether, polyglyceryl laurate (5), polyoxyethylene distyrylated phenyl ether, polyglyceryl laurate (10), polyoxyethylene coconut oil fatty acid sorbitan, polyoxyethylene sorbitan monolaurate (20E.O.), polyglyceryl myristate ( 5), polyoxyethylene polyoxypropylene cetyl ether (20E.O.) (8P.O.), polyoxyethylene tribenzylphenyl ether, polyoxyethylene sorbitan monooleate (20E.O.), sucrose oleic acid One or more selected from esters, polyethylene glycol monostearate (40 E.O.).

Images