JP2020033343A - Bee capture agent - Google Patents

Abstract

To provide: a bee capture agent that can reduce the time it takes for captured bees to die; a bee capture device containing said capture agent; and a bee capture method using said bee capture device.SOLUTION: Provided are: a bee capture agent containing a surfactant (A) (for example: as a non-ionic surfactant, polyglycerin fatty acid ester, polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene aryl ether, polyoxyethylene sorbitan fatty acid ester, polyethylene glycol fatty acid ester, sucrose fatty acid ester; and as an anionic surfactant, alkyl sulfoacetate) as an active ingredient and in which the surfactant (A) has an osmotic force of 2000 seconds or less; a bee capture device containing said capture agent; and a bee capture method using said bee capture device.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a bee trap, a bee trap containing the trap, and a bee trap method using the bee trap. More specifically, the present invention relates to an invention characterized in that a surfactant having a specific penetrating power is used as an active ingredient.

Conventionally, various pest traps are known, for example, cockroaches, and pest attracting traps targeting small flying pests such as flies are commonly used in ordinary households, restaurants, factories, and the like. I have.
Also, bee traps of various shapes have been proposed. Specifically, Patent Literature 1 describes a trap in which an attracting capture composition for attracting a bee is housed inside a cup-shaped container, and a lid having a bee entrance is mounted on the upper part of the container. I have. Patent Literatures 2 and 3 disclose techniques for improving the capture efficiency of a bee by adjusting the visible light transmittance and the color of a capture device. Patent Literature 4 describes an arrangement in which a bee entrance is disposed in the upper central portion of the trap in order to prevent the bee once trapped from escaping from the trap. Furthermore, Patent Document 5 describes that by using a bee-inducing component having a specific sugar content, a fermentation odor due to putrefaction is generated to improve the capture efficiency.

  However, if a liquid attractant is used in these bee traps, it takes time for the bee to fall even if it falls into the liquid attractant, during which time the bee is trapped in liquid. There was a problem that the fish escaped from the container by swimming in the capturing agent, and the capturing efficiency was reduced. In addition, even if a bee is killed, there is another problem that a dead body of the bee floats on the liquid level of the liquid attractant, and this becomes a scaffold for another bee to promote escape.

JP-A-2004-073144 JP 2007-174964 A JP 2009-247236 A JP 2014-103933 A JP 2007-176853 A

  An object of the present invention is to reduce the time required for a captured bee to die.

  The present inventor has conducted intensive studies in order to solve the above-mentioned problems, and as a result, in a surfactant having a specific penetrating power, not only shortening the time until a bee is killed, but also effective use of the surfactant Among the bee trapping agents used as components, it was found that dead carcasses settled out, and the above-mentioned problem was solved by using a surfactant having this specific penetrating power as an active ingredient and a bee trapping agent. Things.

The present invention specifically has the following matters.
1. A bee trap containing the following surfactant (A) as an active ingredient,
Surfactant (A) is a bee trap that has an osmotic force of 2000 seconds or less.
2.1. A bee trap according to claim 5, wherein the bee trap is contained in a container.
3.2. A method for capturing bees, comprising using the bee trap described in 1 above.

Since the time until the bee is lethal is short, the bee trapping agent of the present invention does not escape from the bee trap and exhibits an excellent bee trapping effect. In addition, the bee trap of the present invention sinks in the liquid of the bee trap, so that the bee dead floats on the liquid surface of the bee trap and serves as a scaffold for another bee to promote escape. That is, it does not prevent another bee from coming into contact with the bee-capturing agent.
In addition, the bee capturing agent of the present invention can be used as a controlling agent capable of reliably controlling attracted bees by combining with a known insecticide, and an effective controlling effect on bees can be easily obtained and useful. It is.

It is a graph which shows the total of the hornet capture number in "a hornet capture effect confirmation test."

Hereinafter, the bee trapping agent of the present invention, a bee trap containing the trapping agent, and a bee trapping method using the bee trap will be described in detail.
The bee trap of the present invention contains a surfactant (A) having a specific penetrating power as an active ingredient.
<About the penetration power of the present invention>
The osmotic force in the present invention means a time (second) measured by a measuring method based on a canvas disk method (ISO-8022-1990) described below. The osmotic force (second) in the invention.
(Measuring method)
At 20 ° C., a wool fabric (coated white fabric for dyeing fastness test: JIS L 0803, hair (wool), size: 200 mm × 200 mm) is fixed in a stretched state on a test piece holding frame of φ150 mm in a stretched state without loosening, and measured. A 1% by weight aqueous solution of the target surfactant is dropped on the surface of the wool fabric at 50 μL to form droplets, and the time until the droplets are absorbed by the wool fabric is measured with a stopwatch.
The end point at which the droplet is absorbed by the wool fabric is determined by the disappearance of specular reflection. Specifically, light of 500 to 1000 lux that can visually confirm the specular reflection of water is applied to the periphery of the droplet, and the specular reflection of the droplet is visually confirmed. As the droplets are gradually absorbed by the wool fabric, the specular reflection of the droplets also weakens, and when the droplets are fully absorbed by the wool fabric, the specular reflection of the droplets also disappears. This makes it possible to objectively determine the end point at which the droplet has been absorbed by the wool fabric. This criterion is a method applied in a water absorption test method for textile products (JIS 1907: 2010).
The active ingredient of the bee trapping agent of the present invention is a surfactant whose osmotic power of a 1% by weight aqueous solution is not more than 2000 seconds at 20 ° C., preferably a surfactant having an osmotic force of not more than 1000 seconds. Surfactants that are sub-seconds or less are more preferred.

The active ingredient of the bee trapping agent of the present invention may be a nonionic surfactant, an anionic surfactant, a cationic surfactant, an amphoteric surfactant as long as the surfactant has an osmotic force of 2000 seconds or less. Any of the surfactants can be used without any particular limitation. Among them, a nonionic surfactant or an anionic surfactant is preferable.
Specifically, for example, as nonionic surfactants, sugar ester type, fatty acid ester type, vegetable oil type, alcohol type, alkylphenol type, polyoxyethylene-polyoxypropylene block polymer type, alkylamine type, bisphenol type And a polyaromatic ring type. Examples of the anionic surfactant include carboxylic acid type, sulfonic acid type, sulfate ester type and phosphate ester type. Examples of the cationic surfactant include an ammonium type and a benzalkonium type. Examples of the amphoteric surfactant include a betaine type.
Among them, preferred nonionic surfactants include polyglycerin fatty acid ester, polyoxyalkylene alkyl ether, polyoxyalkylene aryl ether, polyoxyalkylene sorbitan fatty acid ester, polyalkylene glycol fatty acid ester, sucrose fatty acid ester and the like. Suitable anionic surfactants include sulfonates.
Particularly preferred nonionic surfactants include polyglycerin fatty acid ester, polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene aryl ether, polyoxyethylene sorbitan fatty acid ester, polyethylene glycol fatty acid ester, Sucrose fatty acid esters are mentioned, and particularly preferred anionic surfactants include alkylsulfoacetates. The surfactant (A) as the active ingredient may be used alone or in combination of two or more.

The bee trapping agent of the present invention is obtained by simply diluting the surfactant (A) having an osmotic power and excellent in osmotic power with water, the surfactant (A) having an osmotic power and an excellent osmotic force, Examples include those formulated with an insecticide and components generally added to the formulation, and those prepared by diluting the formulated product with water. Above all, as the bee trapping agent of the present invention, the one obtained by formulating the surfactant (A) having excellent osmotic power having the above-mentioned osmotic power and other components is compact and has excellent storage stability. It is advantageous during storage. Examples of the formulation type include liquid formulations such as oils, emulsions, wettable powders and flowables (suspension in water, emulsions in water, etc.), and solid formulations such as microcapsules, powders, granules and tablets. No. Above all, liquid preparations are preferred in that they are easily diluted with water and have little undissolved residue. As the water used for dilution, purified water, tap water, ion-exchanged water, distilled water, filtered water, sterilized water, groundwater, well water, and the like are used.
The bee-capturing agent of the present invention preferably contains 0.005% by weight or more of a surfactant (A) having the above-mentioned penetrative power and having excellent penetrability, based on the whole bee-capturing agent. More preferably, the content is 0.01% by weight or more, particularly preferably 0.05% by weight or more. If the content of the surfactant (A) having the above-mentioned osmotic power and having an excellent osmotic power is less than 0.005% by weight based on the whole bee-capturing agent, bees satisfying the user are sufficient. The capture effect cannot be sufficiently exerted. If the content exceeds 5% by weight, problems in formulation such as solubility will arise, so the content is preferably 5% by weight or less.

  By using the bee capturing agent of the present invention in combination with a known insecticide, it can be used as a controlling agent capable of reliably controlling the attracted bees. Known insecticides which can be used in combination, for example, natural pyrethrins, allethrin, resmethrin, furamethrin, prallethrin, terallethrin, phthalthrin, phenothrin, permethrin, cyphenothrin, cypermethrin, Transfluthrin, metofluthrin, profluthrin, imiprothrin, empenthrin, etofenprox Pyrethroid compounds such as, silafluofen, meperfluthrin and dimefluthrin; Carbamate compounds such as propoxur and carbaryl; Organic phosphorus compounds such as fenitrothion and DDVP; Oxadiazole compounds such as methoxadiazon; Phenylpyrazole compounds such as fipronil; Amidoflumet and the like Sulfonamide compounds; Neonicotinoids such as imidacloprid and dinotefuran Insect juvenile hormone-like compounds such as pyriproxyfen, methoprene, and hydroprene; anti-juvenile hormone-like compounds such as plecosene; chitin synthesis inhibitors such as novalron and diflubenzuron; Various insecticides such as essential oils such as clove oil can be mentioned, and synergists such as sinepirin and piperonyl butoxide can also be used in combination. Insect growth regulators such as insect juvenile hormone-like compounds, anti-juvenile hormone-like compounds, and chitin synthesis inhibitors with low repellency can also be suitably used in combination.

  Among these known insecticides, when the bee-capturing agent and the insecticide of the present invention are used in a mixture, the water solubility at 20 ° C. in order to obtain the lethal rate and the sedimentation effect of the present invention is obtained. Is preferably 100 ppm or more. Examples of insecticides having such water solubility include, for example, acephate, bamidione, methidathion (DMTP), fenobcarb (BPMC), ethiophencarb, cartap, thiocyclam, imidacloprid, thiacloprid, cyromazine, phosthiazate, acetamiprid, thiamethoxam AC, carbaryl N , Clothianidin, pymetrozine, dinotefuran and the like. Among these, for example, dinotefuran (water solubility at 20 ° C .: about 54000 ppm), thiamethoxam (water solubility at 20 ° C .: about 4100 ppm), imidacloprid (water solubility at 20 ° C .: about 510 ppm), fenobucarb (BPMC, water solubility at 20 ° C.) Those having a water solubility of 500 ppm or more at 20 ° C. (solubility: about 610 ppm) are more suitable as insecticides to be combined with the bee trap of the present invention.

  By using the bee trapping agent of the present invention in combination with a known attracting component, bees can be attracted and reliably captured. Known inducing ingredients include, for example, balsamic vinegar, apple vinegar, rice vinegar, brown rice vinegar, lees vinegar, soy vinegar, black vinegar, wine vinegar, sudachi vinegar, red vinegar, persimmon vinegar, malt vinegar, purple potato vinegar, sugarcane Vinegars such as vinegar, lactic acid products (lactic acid bacteria drinks, etc.), sugars, starch sugars, honey, fruits, fruit processed products, fruit juices, juice drinks, fruit wines, etc., liquors, sake lees, seafood, seafood processed products, seafood It may be based on extracts, meat, processed meat products, meat extracts, spices and the like. Among the above attractants, liquid ones are particularly preferred. Furthermore, if a moisturizing component such as an aliphatic polyhydric alcohol such as glycerin, a sugar alcohol such as sorbitol, or a thickening polysaccharide such as xanthan gum is included, the effect of the attracting component can be exhibited for a long period of time.

  The bee trap of the present invention can be formulated by incorporating components generally added to the preparation within a range not to impair the effects of the present invention. Examples of components generally added to the preparation include stabilizers, preservatives, coloring agents, accidental ingestion inhibitors, liquid carriers and the like. Examples of stabilizers include antioxidants such as dibutylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), ascorbic acid, and the like. Examples of preservatives include sorbic acid, sorbates, parahydroxybenzoates, thiabendazole and the like. Examples of the coloring agent include caramel pigment, gardenia pigment, anthocyanin pigment, safflower pigment, flavonoid pigment, red No. 2, red No. 3, yellow No. 4, yellow No. 5, and the like. Examples of the accidental ingestion preventing agent include denatonium benzoate.

  Examples of the liquid carrier used in the formulation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, benzyl alcohol, ethylene glycol, etc.), ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, diethylene glycol). Monoethyl ether, propylene glycol monomethyl ether, tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, ethyl lactate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatics Or aliphatic hydrocarbons (xylene, toluene, alkylnaphthalene, phenylxylylethane, kerosene Light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (acetonitrile, isobutyronitrile, etc.), sulfoxides (dimethylsulfoxide, etc.), heterocyclic solvents (sulfolane) , Γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, 1,3-dimethyl-2-imidazolidinone), acid amides (N, N- Dimethylformamide, N, N-dimethylacetamide, etc.), alkylidene carbonates (propylene carbonate, etc.), vegetable oils (soy oil, cottonseed oil, etc.), vegetable essential oils (orange oil, hyssop oil, lemon oil, etc.), and water.

The bees in which the bee-capturing agent of the present invention exhibits an excellent capturing effect are mainly those belonging to the wasp family (Vespidae). And those belonging to eye pests. Above all, as the wasp family wasps, bees belonging to the wasp subfamily (Vespinae) and the wasp subfamily (Polistinae) can be mentioned.
Examples of wasps belonging to the hornet subfamily include giant hornets, hornets, hornets, hornets, hornets, hornets, hornets, black hornets, hornets, and hornets.
Also, as the bees belonging to the subfamily B. subfamily, for example, Japanese paper wasp, Seguro paper wasp, phthalmon paper wasp, Togariphtamon paper wasp, Yamato paper wasp, Kibosia paper wasp, core paper wasp, Pleurotus wasp, Pseudomonas wasp, etc. Indigenous species can be mentioned. In addition to these indigenous species, hornets that have invaded Tsushima and Kitakyushu can also be mentioned. Although the aggressiveness and toxicity of paper wasps are smaller than that of wasps, they are nesting in private houses, and there are some deaths from bites, so they are the same as hornets in terms of control.
Among these bees, the bee capturing agent of the present invention has a body length of 10 mm or more and 45 mm or less, preferably a body length of 10 mm or more and 30 mm or less, more preferably a body length of 10 mm or more and 25 mm or less. Demonstrate excellent trapping effect against bees.

<About the bee trap>
The bee trap of the present invention may be housed in a container and used as a bee trap.
The shape of the container to be housed is not limited as long as the bee-capturing agent of the present invention can be housed therein, and may be any form suitable for the place and method of use. The material of the container is not particularly limited as long as it is a material such as glass, metal, plastic, or the like, or a special paper having a waterproof or water-repellent function such that the bee trap of the present invention does not leak from the container. .
As an example of the mode of the container, it is preferable that the container has a cover that covers an opening of the container, and either the lid or the container has an opening through which a bee enters. The opening may have any size or shape that allows the bees to easily enter the container, and the dimensions (diameter, width, and length) of the opening are preferably set to 20 mm or more and 35 mm or less, and 25 mm or more to 30 mm. It is more preferable to set the following. If the opening is smaller than 20 mm, a large bee such as a giant hornet will not easily enter through the opening, and the capture efficiency will decrease. Further, if the opening is larger than 35 mm, butterflies, moths, beetles and the like other than the bees enter the container. It is preferable to form a plurality of openings in the container so that bees can easily enter the container. The number of openings depends on the size of the container, but two or more and five or less openings may be formed. preferable. Further, in order to prevent a bee that has entered the container from easily escaping out of the container, the opening is preferably shaped like a funnel.
The container may be provided with a transparent or translucent window equivalent part so that the number of bees captured can be visually observed, or may be a transparent or translucent container, making it difficult to see the captured bees and suppressing discomfort. it can.
When the bee trap is installed outdoors, rainwater or the like is infiltrated and the bee trapping agent of the present invention is diluted, and in order to prevent the bee trapping efficiency from being reduced, the bee trap has a space with respect to the opening, such as rainwater. An embodiment provided with a cover for preventing intrusion is preferable.
It is preferable that the bee trap of the present invention is used while being protected from direct sunlight as much as possible, hung about 1 to 3 m from the ground, or fixed to a fence.

Hereinafter, the present invention will be described in more detail by way of prescription examples and test examples, but the present invention is not limited to these examples.
In the examples, parts mean parts by weight unless otherwise specified.

<Penetration confirmation test>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a 1% by weight uniform aqueous solution, which was used as a test sample.
(2) Measurement of Penetration At 20 ° C., a wool cloth (coated white cloth for dye fastness test: JIS L 0803 compliant, wool (wool), size: 200 mm × 200 mm) was subjected to a 150 mm stainless steel Petri dish and a plastic test piece. It was fixed in a stretched state with a holding frame, placed between a light source (935 lux) and an observer, and a pipetman for dropping a test sample was set so that the distance from the tip to the surface of the wool fabric was 10 mm. The observer observed from an angle at which specular reflection by water can be surely confirmed. A test sample was dropped by 50 μL from a pipette man, and the specular reflection of the droplet formed on the surface of the wool fabric was observed. The time (second) from when the test sample reaches the surface of the wool fabric to when the wool fabric absorbs all the droplets (the specular reflection disappears) and only the wetness remains on the surface of the wool fabric, Measured with a stopwatch. The measurement was performed five times, and the average value was used as the penetrating force (second) of the surfactant.

<Beeh lethal effect confirmation test 1>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a 0.06% by weight uniform aqueous solution, which was used as a test sample for a bee trap.
(2) Test method Each test sample was poured into a container (92 mm in diameter, 131 mm in height, 58 mm in bottom diameter, manufactured by Tokan Kogyo Co., Ltd.) up to a height of 100 mm from the bottom surface. One bee (hereinafter, referred to as "test bee") having a body length of about 10 to 15 mm was placed on the test sample liquid surface, and the time until the behavior stopped was measured. This test was repeated three times, and the average was taken as the bee lethal time (seconds).

<Wasp drowning effect confirmation test 1>
(1) Preparation of Test Sample Each of the following surfactants 1 to 21 was mixed with water to obtain a 0.06% by weight uniform aqueous solution, which was used as a test sample for a bee trap.
(2) Test method Each test sample was poured into the same container as in the above “Bee lethal effect confirmation test 1” to a height of 100 mm from the bottom surface. Seven test bees were placed on the test sample liquid surface, and the number of drowned test bee carcasses 24 hours later was confirmed. Here, drowning means that the dead body of the test bee has settled to the bottom of the container, and does not mean that it is floating below the water surface or in the liquid.
The drowning rate (%) was calculated from the following equation (1).
Formula (1): Drowning rate (%) = 100 × (test bee settled to container bottom) / (test bee number 7)

The osmotic power (sec), bee lethal time (sec), and drowning rate (%) of each test sample are shown together. In accordance with the following [Evaluation Criteria] for bee lethal time (second) and drowning rate (%), the results of each evaluation are also shown in Table 1 below.
[Evaluation criteria for bee lethal time (sec)]
A: Bee lethal time (seconds) is less than 200 seconds B: Bee lethal time (seconds) is 200 seconds or more and less than 400 seconds C: Bee lethal time (seconds) is 400 seconds or more and less than 600 seconds D: Bee lethal time (seconds) Was 600 seconds or more. The above criteria A to C for bee lethal time (seconds) were judged to be practical.
[Evaluation criteria for drowning rate (%)]
A: Drowning rate (%) is 80% or more B: Drowning rate (%) is 65% or more and less than 80% C: Drowning rate (%) is 50% or more and less than 65% D: Drowning rate (%) Is less than 50% or escape ×: means escaped from the container containing the test sample The above evaluation criteria A to C for the drowning rate (%) were judged to be practical.

The details of the surfactant used are as follows.
Surfactant 1: polyglyceryl laurate (10), HLB: 15.5
Surfactant 2: polyoxyethylene alkyl (C12-14) ether, HLB: 13.5
Surfactant 3: sodium lauryl sulfoacetate Surfactant 4: sucrose laurate, HLB: 16
Surfactant 5: sucrose myristic acid ester, HLB: 16
Surfactant 6: polyoxyethylene tristyrylated phenyl ether, HLB: 12
Surfactant 7: polyglyceryl laurate (5), HLB: 15.8
Surfactant 8: polyoxyethylene distyrylated phenyl ether, HLB: 12.8
Surfactant 9: polyglyceryl laurate (10), HLB: 17.1
Surfactant 10: polyoxyethylene coconut oil fatty acid sorbitan, HLB: 16.7
Surfactant 11: polyoxyethylene sorbitan monolaurate (20EO), HLB: 16.7
Surfactant 12: polyglyceryl myristate (5), HLB: 15.4
Surfactant 13: polyoxyethylene polyoxypropylene cetyl ether (20EO) (8PO), HLB: 12.5
Surfactant 14: polyoxyethylene tribenzyl phenyl ether, HLB: 13.2
Surfactant 15: polyoxyethylene sorbitan monooleate (20EO), HLB: 15.0
Surfactant 16: sucrose oleate, HLB: 15
Surfactant 17: polyethylene glycol monostearate (40EO), HLB: 17.5
Surfactant 18: polyoxyethylene phytostyrene, HLB: 12.5
Surfactant 19: polyoxyethylene phytostyrene, HLB: 18.0
Surfactant 20: polyoxyethylene hydrogenated castor oil, HLB: 12.5
Surfactant 21: polyoxyethylene hydrogenated castor oil, HLB: 14.0

  As shown in Table 1, the bee trapping agent containing surfactants 1 to 17, which are surfactants (A) having an osmotic power of 2000 seconds or less as an active ingredient, has a short time until bees are killed, Moreover, it was confirmed that the dead carcasses drowned in the liquid of the bee trap. In particular, it has been found that the bee trapping agent of the present invention containing surfactants 1 to 16 having an osmotic power of 1000 seconds or less as an active ingredient exhibits an excellent bee trapping effect. In the surfactants 18 and 19, although bees were introduced into the test sample, the bees escaped from the test sample and did not result in death. Therefore, "-" in Table 1 means that the time of lethality and the drowning rate could not be measured for the above reason.

<Bee lethal effect confirmation test 2>
(1) Preparation of test sample A bee-capturing agent stock solution was prepared by combining bee-inducing components (liquor, vinegar, sugar, etc.) so that the content of the surfactant 1 was 0.3% by weight. The test solution was prepared by diluting the stock solution five times with water. Further, a comparative test sample containing no surfactant 1 was prepared in the same manner as the test sample in the example.
(2) Test method A test was performed in the same manner as in the above “Bee lethal effect confirmation test 1”, and the time until the behavior stopped was measured. This test was repeated three times, and the average was taken as the bee lethal time (seconds). The results obtained are shown in Table 2 with the results obtained using the test sample in Example 1 as Comparative Example 1 and the results obtained using the test sample in Comparative Example as Comparative Example 1. The evaluation criteria were the same as in the above “Bee lethal effect confirmation test 1”.

<Wasp drowning effect confirmation test 2>
(1) Preparation of Test Specimens The test specimens of the examples of the above "Test for confirming the lethal effect of bees 2" and the test specimens of the comparative examples were used.
(2) Test method A test was performed in the same manner as in the above “Wasp drowning effect confirmation test 1” except that the number of drowning of the dead carp of the test wasp was confirmed 84 hours later, and the drowning rate (%) was calculated did. The results obtained are shown in Table 2 with the results obtained using the test sample in Example 1 as Comparative Example 1 and the results obtained using the test sample in Comparative Example as Comparative Example 1. Note that the evaluation criteria were the same as in the above “Beeh drowning effect confirmation test 1”.

  As shown in Table 2, in Example 1 in which surfactant 1 of the present invention, which is surfactant (A) having a osmotic force of 2000 seconds or less, and an attractant, the time until the bee was killed was used. Was short, and the dead body of the bee was found to drown in the solution of the bee trap. On the other hand, in the case of Comparative Example 1, which was the attractant containing no surfactant 1, although the bee drowned, the time was extremely long. Furthermore, the dead body of the bee hardly drowned in the liquid of Comparative Example 1 and was in a state of floating on the liquid surface. From this, when the comparative example 1 is used in an actual outdoor scene, even if a bee is attracted, the bee that is captured first and floats on the liquid surface of the comparative example 1 becomes a scaffold, and It is presumed that the bee attracted from is likely to escape without being captured and drowned in the liquid of Comparative Example 1.

<Wasp drowning / lethal effect confirmation test>
(1) Preparation of Test Specimen The surfactant 1 was mixed with water to obtain a 0.1% by weight uniform aqueous solution, and this was used as a test sample for an example of a bee trap.
(2) Test method The test sample was poured into a container (diameter 92 mm, height 131 mm, bottom diameter 58 mm, manufactured by Tokan Kogyo Co., Ltd.) up to a height of 100 mm from the bottom surface. A single wasp (hereinafter referred to as "test bee") having a body length of about 20 to 25 mm was placed on the test sample liquid surface, and the time until drowning was measured. This test was repeated twice, and the average was taken as the bee lethal time (seconds).
Also, 24 hours after the drowning, it was confirmed whether the dead body of the test bee drowned to the bottom of the container, and the drowning rate (%) was calculated in the same manner as in the above “Bee drowning effect confirmation test 1”.
Example Using the test sample as Example 2, Table 3 summarizes the bee lethal time (seconds), the drowning rate (%), and the evaluation results. Note that the evaluation criteria were the same as in the above “Beeh drowning effect confirmation test 1”.

  As shown in Table 3, Example 2 containing the surfactant (A) having a penetrating power of 2,000 seconds or less according to the present invention has a short time to death even for paper wasps. Moreover, it was confirmed that the dead body of the bee drowned in the liquid of the bee trap.

<Wasp capture effect confirmation test>
In order to confirm the capturing effect of the bee capturing agent of the present invention on a hornet, a wasp capturing effect confirmation test was performed outdoors. As a reference example, a commercially available trap (commercial product A) containing a bee attractant was used.
(1) Preparation of Example Sample The test sample of the example of the above "Test for confirming the effect of bee lethal effect 2" was used, and this was mixed with the commercially available product A in a bee trap containing no commercially available bee attractant. Example 3 was injected so as to have the same liquid volume.
(2) About the test method The above Example 3 and the reference example were set at a height of 2 m from the ground in each of the thicket forest (test zone 1) and the garden near the building (test zone 2). Cumulative catch was observed over a four week period (May 2018). The test was performed at the above two locations, and the total was defined as the total number of wasps captured.
Table 4 and FIG. 1 show the total number of hornets captured in Examples 3 and Reference Examples in Test Groups 1 and 2, and Table 5 shows the types of hornets captured.

As shown in Table 4 and FIG. 1, the bee trap containing the bee trap of the present invention (Example 3) was compared with the commercial product of the reference example at the initial stage of the first to second weeks. It was confirmed that the effect was very excellent, and that about 1.7 times of bees could be captured by the fourth week. This result is considered to be an effect exerted because the bee trapping agent of the present invention has a surfactant having an excellent penetrating power and a short time until the bee is killed as an active ingredient. In addition, the bee trap of the present invention (Example 3) has an effect that a dead bee sediments on the bee trap, so that the dead bee floats on the liquid surface and escapes from another bee that has entered the trap. It is probable that the number of bees captured has increased steadily from the initial stage of the start of the capture because there is no stepping stone.
When the inside of the bee trap was actually observed, the bee trap of the present invention showed that the captured bees drowned on the bottom of the trap, but the commercial products of the reference examples were not only bees but also moths and moths. Flyes were also captured, and all their carcasses were floating on the capture liquid level. It is probable that, because the dead bodies floating on the liquid level of the capture liquid covered the liquid level, the capture of new bees was hindered, and the number of bees captured was small. The bee trap of the present invention also sediments the dead carcasses of other insects such as moths and flies, and thus can obtain an excellent bee trapping effect over a long period without hindering the capture and drowning of new bees. Can be useful.
Furthermore, the commercial product of the reference example has the drawback that the fly flies lay eggs, and the fly flies are generated in large quantities in the trap and the appearance is dirty. On the other hand, in the bee trap of the present invention, since the captured fly is drowned immediately and the dead body is settled, it is also apparent that the fly is not mass-produced due to spawning, and that it can be used cleanly. It became.
Further, as shown in Table 5, the wasp trap containing the bee trapping agent of the present invention (Example 3) was larger than the commercial product of the reference example in the form of a large wasp (about 25 to 45 mm in length) and a hornet wasp (body length of 22). It was confirmed again that the carnivorous bees with high aggressiveness, such as the Japanese hornet (approximately 17-28 mm), were excellent in capturing bees.

Since the time until the bee is lethal is short, the bee trapping agent of the present invention does not escape from the bee trap and exhibits an excellent bee trapping effect. In addition, the bee trap of the present invention sinks in the liquid of the bee trap, so that the bee dead floats on the liquid surface of the bee trap and serves as a scaffold for another bee to promote escape. That is, it does not prevent another bee from coming into contact with the bee-capturing agent.
In addition, since the bee trapping agent of the present invention drowns insects other than the captured bees and sediments the dead bodies, for example, there is no outbreak of fly flies, etc. Can be maintained.

Claims (3)

A bee trap containing the following surfactant (A) as an active ingredient,
Surfactant (A) is a bee trap that has an osmotic force of 2000 seconds or less.   A bee trap, wherein the bee trap according to claim 1 is stored in a container.   A bee trapping method, comprising using the bee trap according to claim 2.