KR20120072894A - Sex pheromone composition for attraction synanthedon bicingulata and manufacturing thereof - Google Patents
Sex pheromone composition for attraction synanthedon bicingulata and manufacturing thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
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Abstract
Description
본 발명은 복숭아유리나방 유인용 성페로몬 조성물 및 이의 제조방법에 관한 것이다.The present invention relates to a pheromone attracting sex pheromone composition and a method for producing the same.
복숭아유리나방(Synanthedon bicingulata)의 유충은 복숭아나무, 벚나무, 자두나무 등의 수간부 조피 밑을 가해하여 껍질과 목질부 사이(형성층)를 먹고 다닌다. 가해부위는 적갈색의 굵은 배설물과 함께 수액이 흘러나와 겉으로 쉽게 눈에 띤다. 어린 유충이 가해시는 수액분비가 적고 가는 똥이 배출되므로 잎말이나방류 피해로 오인하기 쉽다. 년 2회 발생하고 유충으로 월동하나 월동유충은 어린유충에서 노숙유충까지 다양하다. 월동유충은 보통 3월상순경부터 활동을 시작하여 가해하며, 이때 어린유충은 껍질 바로 밑에 있기 때문에 방제하기 쉬우나, 성장할 수록 껍질밑 깊숙히 들어가기 때문에 방제가 곤란하다.Peach Glass Moth ( Synanthedon) The larvae of bicingulata are fed under the bark of the bark of peach trees, cherry trees, and plums, and eat between the bark and the wood. The affected area is easily visible from the outflow of sap along with reddish brown coarse excrement. The young larvae are less likely to produce sap and thin shit is discharged, so it is easy to mistake them for damage to leaves or discharge. It occurs twice a year and winters as a larva, but wintering larvae vary from young to homeless larvae. Wintering larvae usually start activities around early March, and the young larvae are easy to control because they are just under the bark, but they are difficult to control because they grow deeper under the bark as they grow.
복숭아유리나방을 방제하기 위한 방법으로는, 성충이 산란하지 못하도록 접촉성 살충제 등을 혼용한 백도제(또는 수성페인트)를 가해부 줄기에 발라주는 방법, 벌레똥이 나오는 곳을 찾아서 철사나 칼로 유충을 직접 잡아주는 방법 또는 살충제를 살포하는 방법 등이 활용되고 있다. 살충제 살포는 월동유충이 활동하는 시기인 3월 중하순에 침투성 살충제를 굵은 가지와 주지를 중심으로 흘러 내리도록 충분히 살포한다. 생육기에는 성충발생기에 유기인계나 합성피레스로이드계 살충제를 살포하고 특히 복숭아 수확이 끝나는 8월 이후 약제살포로 난 및 유충을 구제하는 것이 효과적이다. 현재 고시된 약제는 없으나 스미치온 등이 효과가 있는 것으로 알려져 있다. 그러나 과다한 살충제의 사용으로 인해 해충의 살충제 저항성 유발, 천적 및 유용곤충의 감소로 생태계 파괴, 잔류독성에 의한 인축독성 등의 문제점이 대두되고 있다. In order to control the peach glass moth, apply white bleach (or water-based paint) mixed with contact insecticide to prevent the adult from spawning, apply it to the dissected stem, find the place where the insect dung comes out and larva with wire or knife. The method of catching them directly or spraying insecticides is used. Insecticides are sprayed sufficiently in the middle of March, when wintering larvae are active, so that the invasive pesticides flow down the thick branches and the main ground. During the growing season, it is effective to spray organophosphorus or synthetic pyrethroid insecticides on adult stages, and to control eggs and larvae by spraying drugs, especially after August when peaches are harvested. There is no drug currently announced, but it is known that smithion and the like are effective. However, due to the use of excessive pesticides, problems such as causing insecticide resistance of pests, reduction of natural enemies and useful insects, destruction of ecosystem, and human toxicity due to residual toxicity have emerged.
이러한 문제를 해결하기 위한 방법으로 성페로몬을 이용하는 방법이 미국과 유럽 등 농업선진국에서 적용되어져 왔다. 성페로몬은 생물의 같은 종내 통신물질인 페로몬의 한가지로 한쪽 성에서 발산하여 상대 성을 유인하여 교미를 하게 하는 통신물질을 말한다. 유기합성 살충제는 곤충을 비특이적으로 박멸하기 위해 많은 양 살포해야 하는 것과는 달리, 성페로몬은 매우 적은 양 예컨대 hr당 10 내지 20g씩 2회 또는 3회 처리로 효과를 지속적으로 유지시켜 사용할 수 있는 장점이 있다. 즉, 성페로몬은 종특이적으로 작용하고 독성이 없으며 다른 생물에는 영향이 없다. 성페로몬이 이용되는 분야는 발생예찰과 대량유살, 교미교란에 의한 방제분야이고 이 중 발생예찰분야는 대상 해충의 발생상황을 지속적으로 조사하여 살충제 처리 필요여부를 판단하고, 필요시 살충제 처리 시점을 정확히 파악할 수 있어 살충제 남용을 방지하고 생태계를 보존할 수 있는 해충종합관리(Integrated Pest Management)의 근간이 되고 있다.In order to solve this problem, the method of using sex pheromone has been applied in developed countries such as the United States and Europe. Sex pheromone is a kind of pheromone that is a communication material in the same species of living things. It is a communication material that emanates from one castle and attracts relatives. Unlike organic pesticides, which must be sprayed in large quantities to non-specifically eradicate insects, sex pheromones have the advantage of being able to sustain their effectiveness in very small amounts, such as 10 to 20 g / hr twice or three times. have. In other words, sex pheromone acts species-specifically, is not toxic, and does not affect other organisms. Sex pheromone is used in the field of outbreak prediction, mass killing, and mating disturbance. Among these, outbreak prediction field continuously investigates the occurrence of pests to determine the need for pesticide treatment and, if necessary, determines the time of pesticide treatment. This is the basis of Integrated Pest Management, which can be pinpointed to prevent abuse of pesticides and preserve ecosystems.
그러나, 복숭아유리나방의 성페로몬 및 이를 이용한 해충 방제 방법에 대해서는 연구가 전무한바, 이에 본 발명자들은 복숭아유리나방 암컷의 성페로몬을 동정하여 수컷 성충의 유인에 최적의 효과를 발휘하는 성페로몬 조성물을 연구개발한 결과, 페로몬 트랩을 이용한 발생예찰로 복숭아유리나방의 발생여부와 발생량을 조사하여 방제약제를 적기에 살포함으로서 복숭아나무의 피해를 줄일 수 있으며, 더 나이가 친환경 해충 방제제인 교미교란제를 제작하여 본 발명을 완성하였다.
However, no studies have been conducted on the sex pheromone of peach glass moth and pest control methods using the same. Therefore, the present inventors have identified a sex pheromone of female peach glass moth, and thus the sex pheromone composition exhibiting an optimal effect on attracting male adults. As a result of the research and development, it is possible to reduce the damage of peach trees by timely spraying the control medicine by investigating the occurrence and amount of peach glass moths by using the pheromone traps. It produced and completed this invention.
본 발명의 목적은 복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 GC-MS 분석결과 (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate)와 (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate)를 유효성분으로 포함하는 복숭아유리나방 유인용 성페로몬 조성물을 제공하는 것이다. An object of the present invention is a peach glass moth ( Synanthedon) bicingulata) Results GC-MS analysis in the female sex pheromone extract (E, Z) -3,13- octa decanoic dienyl acetate ((E, Z) -3,13- octadecadienyl acetate) and (Z, Z) -3, It is to provide a pheromone pheromone composition for peach glass moth attracting 13-octadecadienyl acetate (( Z, Z ) -3,13-octadecadienyl acetate) as an active ingredient.
본 발명의 또 다른 목적은 복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 GC-MS 분석결과 (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate)와 (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate)를 유효성분으로 포함하는 복숭아유리나방 유인용 성페로몬 조성물을 제조하는 방법을 제공하는 것이다.
Another object of the present invention is a peach glass moth ( Synanthedon bicingulata) Results GC-MS analysis in the female sex pheromone extract (E, Z) -3,13- octa decanoic dienyl acetate ((E, Z) -3,13- octadecadienyl acetate) and (Z, Z) -3, It is to provide a method for preparing a pheromone pheromone composition for peach glass moths containing 13-octadecadienyl acetate (( Z, Z ) -3,13-octadecadienyl acetate) as an active ingredient.
상기 과제 해결을 위해, 본 발명의 일 구체예는 복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 GC-MS 분석결과 (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate)와 (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate)를 유효성분으로 포함하는 복숭아유리나방 유인용 성페로몬 조성물을 제공한다. 여기서, 상기 2가지 성분의 비율은 30:70~50:50, 더욱 바람직하게는 40:60인 것이 바람직하다. To solve the above problems, one embodiment of the invention is a glass peach moth (Synanthedon bicingulata) Results GC-MS analysis in the female sex pheromone extract (E, Z) -3,13- octa decanoic dienyl acetate ((E, Z ) -3,13-octadecadienyl acetate) and (Z, Z) -3,13- octa decanoic dienyl acetate ((Z, Z) -3,13- octadecadienyl acetate) the glass peach moth sex bait comprising as an active ingredient It provides a pheromone composition. Here, the ratio of the two components is preferably 30:70 to 50:50, more preferably 40:60.
본 발명의 일 구체예는 복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 GC-MS 분석결과 (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate)와 (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate)를 유효성분으로 포함하는 복숭아유리나방 유인용 성페로몬 조성물을 제조하는 방법을 제공한다. 여기서, 상기 2가지 성분의 비율은 30:70~50:50, 더욱 바람직하게는 40:60인 것이 바람직하다.
One embodiment of the present invention is a peach glass moth ( Synanthedon bicingulata) Results GC-MS analysis in the female sex pheromone extract (E, Z) -3,13- octa decanoic dienyl acetate ((E, Z) -3,13- octadecadienyl acetate) and (Z, Z) -3, Provided is a method for preparing a pheromone pheromone composition for peach glass moth attracting 13-octadecadienyl acetate (( Z, Z ) -3,13-octadecadienyl acetate) as an active ingredient. Here, the ratio of the two components is preferably 30:70 to 50:50, more preferably 40:60.
본 발명에 따른 조성물을 통하여 발생예찰 도구인 페로몬 트랩과 친환경 해충 방제제인 교미교란제를 제작할 수 있어, 복숭아유리나방에 의한 복숭아나무의 피해를 줄일 수 있다.
Through the composition according to the present invention can produce a pheromone trap and a mating disturbance agent of environmentally friendly pest control agent, it can reduce the damage of peach tree by peach glass moth.
도 1은 복숭아유리나방 암컷 성페로몬샘 추출물의 GC-MS 분석 결과그래프이다.1 is a graph of the results of GC-MS analysis of peach glass moth female sex pheromone extract.
이하, 본 발명을 하기의 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
실시예Example 1. One. 복숭아유리나방Peach Glass Moth 암컷의 Female 성페로몬St. Pheromones 성분 ingredient
복숭아유리나방(Synanthedon bicingulata)의 암컷 성페로몬 샘에서 성페로몬을 추출하였다. 추출한 물질을 화학 분석한 결과, (Z)-13-옥타데세닐 아세테이트((Z)-13-octadecenyl acetate), (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate), (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate), (E,Z)-2,13-옥타데카디에닐 아세테이트((E,Z)-2,13-octadecadienyl acetate), (E,Z)-3,13-옥타데카디엔-1-올((E,Z)-3,13-octadecadien-1-ol) 및 (Z,Z)-3,13-옥타데카디엔-1-올((Z,Z)-3,13-octadecadien-1-ol) 6가지 성분이 동정되었다.
Peach Glass Moth ( Synanthedon) Sex pheromone was extracted from female sex pheromone gland of bicingulata ). After a chemical analysis of the extracted substances, (Z) -13- octadecenyl acetate ((Z) -13-octadecenyl acetate ), (E, Z) -3,13- octa decanoic dienyl acetate ((E, Z) (3,13-octadecadienyl acetate), ( Z, Z ) -3,13-octadecadienyl acetate (( Z, Z ) -3,13-octadecadienyl acetate), ( E, Z ) -2,13-octa deca-dienyl acetate ((E, Z) -2,13- octadecadienyl acetate), (E, Z) -3,13- octadecadienoic-1-ol ((E, Z) -3,13- octadecadien-1 -ol) and ( Z, Z ) -3,13-octadecadien-1-ol (( Z, Z ) -3,13-octadecadien-1-ol) six components were identified.
실시예Example 2. 2. 복숭아유리나방Peach Glass Moth 성페로몬St. Pheromones 성분의 수컷 유인효과 Male Attracting Effect of Ingredients
복숭아유리나방의 성페로몬 추출물의 6가지 성분을 GC-MS 분석한 결과 도 1과 같은 결과를 나타내었다. 도 1의 결과를 바탕으로 표 1과 같은 조성물로 실험을 한 결과, 야외에서 복숭아유리나방 수컷을 유인하기 위해서는 (E,Z)-3,13-옥타데카디에닐 아세테이트((E,Z)-3,13-octadecadienyl acetate)와 (Z,Z)-3,13-옥타데카디에닐 아세테이트((Z,Z)-3,13-octadecadienyl acetate)의 2가지 성분이 반드시 필요하다는 사실을 알 수 있었다.GC-MS analysis of the six components of the sex pheromone extract of Peach glass moth showed the same result as FIG. Based on the results of FIG. 1, the results of the experiment as shown in Table 1, in order to attract male peach glass moth males in the open air ( E, Z ) -3,13-octadecadienyl acetate (( E, Z )- it was found that the two components of the 3,13-octadecadienyl acetate) and (Z, Z) -3,13- octa decanoic dienyl acetate ((Z, Z) -3,13- octadecadienyl acetate) that required .
또한, (E,Z)-3,13-옥타데카디에닐 아세테이트와 (Z,Z)-3,13-옥타데카디에닐 아세테이트의 2가지 성분의 비율을 달리하여 실험을 시행하였다. 그 결과는 다음 표 2와 같았다.In addition, the experiment was conducted by varying the ratio of the two components of ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate. The results were as shown in Table 2 below.
실시예Example 3. 3. 복숭아유리나방Peach Glass Moth 성페로몬을St. Pheromones 이용한 교미교란 방제 Mating disturbance control
복숭아유리나방 성페로몬 성분인 ((E,Z)-3,13-옥타데카디에닐 아세테이트와 (Z,Z)-3,13-옥타데카디에닐 아세테이트를 단독 또는 혼합하여 폴레에틸렌 튜브(polyethylene tube) 등에 넣은 교미교란제를 복숭아 나무에 설치해두었다. 그 결과, 과수원에 자연 발생하는 복숭아유리나방의 암컷과 수컷의 정상적인 교미행동이 방해 또는 지연되어 다음 세대의 유충에 의한 복숭아나무 피해가 감소함을 확인하였다.Pethylene glass moth sex pheromone component (( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate alone or mixed The copulation disturbances were placed on the peach tree, which prevented or delayed the normal mating behavior of peach glass moths and males naturally occurring in the orchard, reducing the damage of the peach tree by the next generation of larvae. Confirmed.
Claims (10)
( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate as active ingredients.
상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트의 비율은 30:70~50:50인 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물.
The method of claim 1,
Peach glass moth, characterized in that the ratio of ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate is 30: 70-50: 50 Attractant sex pheromone composition.
상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트의 비율은 40:60인 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물.
The method of claim 2,
The ratio of ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate is 40:60. .
상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트는 복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 분리된 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물.
The method of claim 1,
The ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate are peach free moths ( Synanthedon). bicingulata ) Peach glass moth attracting sex pheromone composition, characterized in that isolated from female sex pheromone extract.
( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate are mixed to prepare a pheromone composition for peach glass moth attraction.
상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트의 비율은 30:70~50:50인 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물을 제조하는 방법.
6. The method of claim 5,
Peach glass moth, characterized in that the ratio of ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate is 30: 70-50: 50 A method of preparing a attractant sex pheromone composition.
상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트의 비율은 40:60인 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물을 제조하는 방법.
The method according to claim 6,
The ratio of ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate is 40:60. How to prepare.
복숭아유리나방(Synanthedon bicingulata) 암컷 성페로몬 추출물에서 상기 (E,Z)-3,13-옥타데카디에닐 아세테이트 및 (Z,Z)-3,13-옥타데카디에닐 아세테이트를 분리하는 단계를 추가로 포함하는 것을 특징으로 하는 복숭아유리나방 유인용 성페로몬 조성물을 제조하는 방법.
The method of claim 5,
Peach Glass Moth ( Synanthedon) bicingulata ) further comprising the step of separating the ( E, Z ) -3,13-octadecadienyl acetate and ( Z, Z ) -3,13-octadecadienyl acetate from the female sex pheromone extract Method for producing a peach glass moth attracting sex pheromone composition.
A copious disturbance composition for controlling peach glass moths comprising ( E, Z ) -3,13-octadecadienyl acetate or ( Z, Z ) -3,13-octadecadienyl acetate or a mixture thereof as an active ingredient .
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