JP7277058B2 - Dna配列のクラスター化のための方法 - Google Patents
Dna配列のクラスター化のための方法 Download PDFInfo
- Publication number
- JP7277058B2 JP7277058B2 JP2020520434A JP2020520434A JP7277058B2 JP 7277058 B2 JP7277058 B2 JP 7277058B2 JP 2020520434 A JP2020520434 A JP 2020520434A JP 2020520434 A JP2020520434 A JP 2020520434A JP 7277058 B2 JP7277058 B2 JP 7277058B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- nucleic acids
- item
- proximity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims description 265
- 108091028043 Nucleic acid sequence Proteins 0.000 title description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 301
- 102000039446 nucleic acids Human genes 0.000 claims description 296
- 108020004707 nucleic acids Proteins 0.000 claims description 296
- 108090000623 proteins and genes Proteins 0.000 claims description 127
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 102
- 230000000295 complement effect Effects 0.000 claims description 95
- 108091034117 Oligonucleotide Proteins 0.000 claims description 94
- 230000003115 biocidal effect Effects 0.000 claims description 73
- 239000011324 bead Substances 0.000 claims description 57
- 229960002685 biotin Drugs 0.000 claims description 51
- 235000020958 biotin Nutrition 0.000 claims description 51
- 239000011616 biotin Substances 0.000 claims description 51
- 238000004132 cross linking Methods 0.000 claims description 47
- 239000007787 solid Substances 0.000 claims description 47
- 239000000758 substrate Substances 0.000 claims description 46
- 238000003752 polymerase chain reaction Methods 0.000 claims description 45
- 102000004169 proteins and genes Human genes 0.000 claims description 33
- 239000003431 cross linking reagent Substances 0.000 claims description 32
- 238000012163 sequencing technique Methods 0.000 claims description 32
- 238000007481 next generation sequencing Methods 0.000 claims description 28
- 238000002372 labelling Methods 0.000 claims description 25
- 238000000746 purification Methods 0.000 claims description 23
- 108700005443 Microbial Genes Proteins 0.000 claims description 21
- 239000003599 detergent Substances 0.000 claims description 21
- 230000000813 microbial effect Effects 0.000 claims description 20
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 239000013612 plasmid Substances 0.000 claims description 18
- 239000002773 nucleotide Substances 0.000 claims description 17
- 108010090804 Streptavidin Proteins 0.000 claims description 16
- 230000027455 binding Effects 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 108090001008 Avidin Proteins 0.000 claims description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 13
- 108091005804 Peptidases Proteins 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 239000004365 Protease Substances 0.000 claims description 12
- 239000004471 Glycine Substances 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000002934 lysing effect Effects 0.000 claims description 10
- 239000012139 lysis buffer Substances 0.000 claims description 10
- 208000015181 infectious disease Diseases 0.000 claims description 8
- 241000700605 Viruses Species 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 230000001018 virulence Effects 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 137
- 239000000523 sample Substances 0.000 description 83
- 108700020796 Oncogene Proteins 0.000 description 59
- 108020004414 DNA Proteins 0.000 description 56
- 102000053602 DNA Human genes 0.000 description 55
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 49
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 49
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 33
- 238000009396 hybridization Methods 0.000 description 31
- 102100031780 Endonuclease Human genes 0.000 description 28
- 108010042407 Endonucleases Proteins 0.000 description 27
- 206010028980 Neoplasm Diseases 0.000 description 27
- 239000003242 anti bacterial agent Substances 0.000 description 24
- 238000002493 microarray Methods 0.000 description 24
- 238000001514 detection method Methods 0.000 description 17
- 230000005291 magnetic effect Effects 0.000 description 17
- 239000000203 mixture Substances 0.000 description 16
- 108091008146 restriction endonucleases Proteins 0.000 description 14
- 108010077544 Chromatin Proteins 0.000 description 13
- 210000003483 chromatin Anatomy 0.000 description 13
- 108020004999 messenger RNA Proteins 0.000 description 13
- 102000040430 polynucleotide Human genes 0.000 description 13
- 108091033319 polynucleotide Proteins 0.000 description 13
- 239000002157 polynucleotide Substances 0.000 description 13
- 108010061982 DNA Ligases Proteins 0.000 description 12
- 102000012410 DNA Ligases Human genes 0.000 description 12
- 108700028369 Alleles Proteins 0.000 description 11
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
- 230000002255 enzymatic effect Effects 0.000 description 11
- 239000011159 matrix material Substances 0.000 description 11
- 201000011510 cancer Diseases 0.000 description 10
- 102000016911 Deoxyribonucleases Human genes 0.000 description 9
- 108010053770 Deoxyribonucleases Proteins 0.000 description 9
- 239000012807 PCR reagent Substances 0.000 description 9
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 9
- 102000003960 Ligases Human genes 0.000 description 8
- 108090000364 Ligases Proteins 0.000 description 8
- 229910019142 PO4 Inorganic materials 0.000 description 8
- 235000021317 phosphate Nutrition 0.000 description 8
- 229920002477 rna polymer Polymers 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 102000035195 Peptidases Human genes 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 241000283690 Bos taurus Species 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 6
- 239000010871 livestock manure Substances 0.000 description 6
- 238000013507 mapping Methods 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 210000004767 rumen Anatomy 0.000 description 6
- 239000004971 Cross linker Substances 0.000 description 5
- 102000004594 DNA Polymerase I Human genes 0.000 description 5
- 108010017826 DNA Polymerase I Proteins 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 210000004962 mammalian cell Anatomy 0.000 description 5
- 235000019419 proteases Nutrition 0.000 description 5
- 230000002441 reversible effect Effects 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 206010009944 Colon cancer Diseases 0.000 description 4
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 4
- 108010067770 Endopeptidase K Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 101000850434 Homo sapiens V-type proton ATPase subunit B, brain isoform Proteins 0.000 description 4
- 101000670953 Homo sapiens V-type proton ATPase subunit B, kidney isoform Proteins 0.000 description 4
- 206010025323 Lymphomas Diseases 0.000 description 4
- 108091093037 Peptide nucleic acid Proteins 0.000 description 4
- 208000000453 Skin Neoplasms Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 102100033476 V-type proton ATPase subunit B, brain isoform Human genes 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 239000002777 nucleoside Substances 0.000 description 4
- 201000000849 skin cancer Diseases 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- DLGOEMSEDOSKAD-UHFFFAOYSA-N Carmustine Chemical compound ClCCNC(=O)N(N=O)CCCl DLGOEMSEDOSKAD-UHFFFAOYSA-N 0.000 description 3
- 108700039887 Essential Genes Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102000043276 Oncogene Human genes 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 208000032839 leukemia Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 230000008823 permeabilization Effects 0.000 description 3
- 102000054765 polymorphisms of proteins Human genes 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000009877 rendering Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 2
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000010839 B-cell chronic lymphocytic leukemia Diseases 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 2
- 108010060248 DNA Ligase ATP Proteins 0.000 description 2
- 102000008158 DNA Ligase ATP Human genes 0.000 description 2
- 201000003741 Gastrointestinal carcinoma Diseases 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 206010021042 Hypopharyngeal cancer Diseases 0.000 description 2
- 206010056305 Hypopharyngeal neoplasm Diseases 0.000 description 2
- 206010023825 Laryngeal cancer Diseases 0.000 description 2
- 206010028729 Nasal cavity cancer Diseases 0.000 description 2
- 206010057444 Oropharyngeal neoplasm Diseases 0.000 description 2
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 2
- 101710086015 RNA ligase Proteins 0.000 description 2
- 206010039491 Sarcoma Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 208000011783 Splenic diffuse red pulp small B-cell lymphoma Diseases 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- DQLATGHUWYMOKM-UHFFFAOYSA-L cisplatin Chemical compound N[Pt](N)(Cl)Cl DQLATGHUWYMOKM-UHFFFAOYSA-L 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 201000006866 hypopharynx cancer Diseases 0.000 description 2
- 201000002313 intestinal cancer Diseases 0.000 description 2
- 206010023841 laryngeal neoplasm Diseases 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 125000003835 nucleoside group Chemical group 0.000 description 2
- 201000005443 oral cavity cancer Diseases 0.000 description 2
- 201000006958 oropharynx cancer Diseases 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 description 2
- 208000037968 sinus cancer Diseases 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 206010062113 splenic marginal zone lymphoma Diseases 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 description 1
- XHCNINMOALIGKM-UHFFFAOYSA-N 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetrazacyclotetradecane Chemical compound CC1CC(C)(C)NCCNC(C)CC(C)(C)NCCN1 XHCNINMOALIGKM-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 208000006468 Adrenal Cortex Neoplasms Diseases 0.000 description 1
- 108010063905 Ampligase Proteins 0.000 description 1
- 206010061424 Anal cancer Diseases 0.000 description 1
- 208000007860 Anus Neoplasms Diseases 0.000 description 1
- 101100316808 Arabidopsis thaliana VHA-E1 gene Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010004593 Bile duct cancer Diseases 0.000 description 1
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 206010005949 Bone cancer Diseases 0.000 description 1
- 208000018084 Bone neoplasm Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 101150038588 CHRNB1 gene Proteins 0.000 description 1
- 101100476566 Campylobacter jejuni aad9 gene Proteins 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 102100029995 DNA ligase 1 Human genes 0.000 description 1
- 101710148291 DNA ligase 1 Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101150105814 ERMN gene Proteins 0.000 description 1
- 206010014733 Endometrial cancer Diseases 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 101100476567 Enterococcus faecalis spc gene Proteins 0.000 description 1
- 101900063352 Escherichia coli DNA ligase Proteins 0.000 description 1
- 101100223892 Escherichia coli sulI gene Proteins 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000022072 Gallbladder Neoplasms Diseases 0.000 description 1
- 208000031852 Gastrointestinal stromal cancer Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 101100077927 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) mrdA gene Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 101000850489 Homo sapiens V-type proton ATPase subunit D Proteins 0.000 description 1
- 208000019758 Hypergammaglobulinemia Diseases 0.000 description 1
- 208000007766 Kaposi sarcoma Diseases 0.000 description 1
- 208000008839 Kidney Neoplasms Diseases 0.000 description 1
- 240000000759 Lepidium meyenii Species 0.000 description 1
- 235000000421 Lepidium meyenii Nutrition 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 208000032271 Malignant tumor of penis Diseases 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 108091092878 Microsatellite Proteins 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 101100406473 Mus musculus Oprm1 gene Proteins 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 208000001894 Nasopharyngeal Neoplasms Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 206010031096 Oropharyngeal cancer Diseases 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 208000002471 Penile Neoplasms Diseases 0.000 description 1
- 206010034299 Penile cancer Diseases 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 208000007913 Pituitary Neoplasms Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 208000033766 Prolymphocytic Leukemia Diseases 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 108090001087 RNA ligase (ATP) Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 206010038389 Renal cancer Diseases 0.000 description 1
- 201000000582 Retinoblastoma Diseases 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 101150071725 SMDT1 gene Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000004337 Salivary Gland Neoplasms Diseases 0.000 description 1
- 206010061934 Salivary gland cancer Diseases 0.000 description 1
- 101100054091 Schizosaccharomyces pombe (strain 972 / ATCC 24843) par2 gene Proteins 0.000 description 1
- 101100403885 Shewanella oneidensis (strain MR-1) nadD gene Proteins 0.000 description 1
- 206010041067 Small cell lung cancer Diseases 0.000 description 1
- 208000021712 Soft tissue sarcoma Diseases 0.000 description 1
- 244000025012 Spondias pinnata Species 0.000 description 1
- 235000005658 Spondias pinnata Nutrition 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
- 201000008736 Systemic mastocytosis Diseases 0.000 description 1
- 208000024313 Testicular Neoplasms Diseases 0.000 description 1
- 206010057644 Testis cancer Diseases 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- 101000803959 Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) DNA ligase Proteins 0.000 description 1
- 208000000728 Thymus Neoplasms Diseases 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 208000002495 Uterine Neoplasms Diseases 0.000 description 1
- 102100033478 V-type proton ATPase subunit D Human genes 0.000 description 1
- 101150009898 VATE gene Proteins 0.000 description 1
- 206010047741 Vulval cancer Diseases 0.000 description 1
- 208000004354 Vulvar Neoplasms Diseases 0.000 description 1
- 208000008383 Wilms tumor Diseases 0.000 description 1
- 230000001780 adrenocortical effect Effects 0.000 description 1
- 239000002168 alkylating agent Substances 0.000 description 1
- 229940100198 alkylating agent Drugs 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 229940126575 aminoglycoside Drugs 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 201000011165 anus cancer Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 210000000013 bile duct Anatomy 0.000 description 1
- 208000026900 bile duct neoplasm Diseases 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 201000000220 brain stem cancer Diseases 0.000 description 1
- 208000002458 carcinoid tumor Diseases 0.000 description 1
- 229960005243 carmustine Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- MYPYJXKWCTUITO-KIIOPKALSA-N chembl3301825 Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)C(O)[C@H](C)O1 MYPYJXKWCTUITO-KIIOPKALSA-N 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 208000006990 cholangiocarcinoma Diseases 0.000 description 1
- 201000010902 chronic myelomonocytic leukemia Diseases 0.000 description 1
- 229960004316 cisplatin Drugs 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000011243 crosslinked material Substances 0.000 description 1
- MLUCVPSAIODCQM-NSCUHMNNSA-N crotonaldehyde Chemical compound C\C=C\C=O MLUCVPSAIODCQM-NSCUHMNNSA-N 0.000 description 1
- MLUCVPSAIODCQM-UHFFFAOYSA-N crotonaldehyde Natural products CC=CC=O MLUCVPSAIODCQM-UHFFFAOYSA-N 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013523 data management Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 208000024519 eye neoplasm Diseases 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 101150064107 fosB gene Proteins 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 230000005021 gait Effects 0.000 description 1
- 201000010175 gallbladder cancer Diseases 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 239000012145 high-salt buffer Substances 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 201000010982 kidney cancer Diseases 0.000 description 1
- 235000012902 lepidium meyenii Nutrition 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 235000019689 luncheon sausage Nutrition 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000026807 lung carcinoid tumor Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 208000019420 lymphoid neoplasm Diseases 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000010208 microarray analysis Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 101150021123 msrA gene Proteins 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000011807 nanoball Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 210000003928 nasal cavity Anatomy 0.000 description 1
- 201000008026 nephroblastoma Diseases 0.000 description 1
- 201000011682 nervous system cancer Diseases 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- -1 nucleoside triphosphates Chemical class 0.000 description 1
- 201000008106 ocular cancer Diseases 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 201000008968 osteosarcoma Diseases 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 101150038610 penA gene Proteins 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 239000008191 permeabilizing agent Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical group 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
- 208000010916 pituitary tumor Diseases 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 239000012066 reaction slurry Substances 0.000 description 1
- 206010038038 rectal cancer Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012508 resin bead Substances 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 201000009410 rhabdomyosarcoma Diseases 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 201000003120 testicular cancer Diseases 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229940040944 tetracyclines Drugs 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 206010046766 uterine cancer Diseases 0.000 description 1
- 208000037965 uterine sarcoma Diseases 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 201000005102 vulva cancer Diseases 0.000 description 1
- 150000003952 β-lactams Chemical class 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023032861A JP2023065620A (ja) | 2017-06-26 | 2023-03-03 | Dna配列のクラスター化のための方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762524815P | 2017-06-26 | 2017-06-26 | |
| US62/524,815 | 2017-06-26 | ||
| PCT/US2018/039454 WO2019005763A1 (en) | 2017-06-26 | 2018-06-26 | METHOD FOR REGROUPING DNA SEQUENCES |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023032861A Division JP2023065620A (ja) | 2017-06-26 | 2023-03-03 | Dna配列のクラスター化のための方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020525046A JP2020525046A (ja) | 2020-08-27 |
| JP2020525046A5 JP2020525046A5 (https=) | 2021-07-26 |
| JP7277058B2 true JP7277058B2 (ja) | 2023-05-18 |
Family
ID=64742178
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020520434A Active JP7277058B2 (ja) | 2017-06-26 | 2018-06-26 | Dna配列のクラスター化のための方法 |
| JP2023032861A Pending JP2023065620A (ja) | 2017-06-26 | 2023-03-03 | Dna配列のクラスター化のための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023032861A Pending JP2023065620A (ja) | 2017-06-26 | 2023-03-03 | Dna配列のクラスター化のための方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US11753637B2 (https=) |
| EP (1) | EP3645547A4 (https=) |
| JP (2) | JP7277058B2 (https=) |
| CA (1) | CA3066745A1 (https=) |
| WO (1) | WO2019005763A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220403371A1 (en) * | 2019-11-15 | 2022-12-22 | Phase Genomics, Inc. | Chromosome conformation capture from tissue samples |
| CN116323973A (zh) * | 2020-08-06 | 2023-06-23 | 上海绾塍生物科技有限公司 | 一种分析经固定的细胞的组分的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016537029A (ja) | 2013-11-18 | 2016-12-01 | エラスムス ユニベルジテート メディッシュ セントルム ロッテルダム | 三次元dna構造におけるヌクレオチド配列の相互作用を分析する方法 |
Family Cites Families (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5871928A (en) | 1989-06-07 | 1999-02-16 | Fodor; Stephen P. A. | Methods for nucleic acid analysis |
| US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| ES2204913T3 (es) | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US6090555A (en) | 1997-12-11 | 2000-07-18 | Affymetrix, Inc. | Scanned image alignment systems and methods |
| US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
| US5571639A (en) | 1994-05-24 | 1996-11-05 | Affymax Technologies N.V. | Computer-aided engineering system for design of sequence arrays and lithographic masks |
| US5795716A (en) | 1994-10-21 | 1998-08-18 | Chee; Mark S. | Computer-aided visualization and analysis system for sequence evaluation |
| US5545531A (en) | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
| US5780613A (en) | 1995-08-01 | 1998-07-14 | Northwestern University | Covalent lock for self-assembled oligonucleotide constructs |
| US5733729A (en) | 1995-09-14 | 1998-03-31 | Affymetrix, Inc. | Computer-aided probability base calling for arrays of nucleic acid probes on chips |
| US6114122A (en) | 1996-03-26 | 2000-09-05 | Affymetrix, Inc. | Fluidics station with a mounting system and method of using |
| EP0902885A4 (en) | 1996-05-16 | 2006-09-27 | Affymetrix Inc | SYSTEMS AND METHODS FOR DETECTION OF BRANDED PRODUCTS |
| US6420108B2 (en) | 1998-02-09 | 2002-07-16 | Affymetrix, Inc. | Computer-aided display for comparative gene expression |
| US6188783B1 (en) | 1997-07-25 | 2001-02-13 | Affymetrix, Inc. | Method and system for providing a probe array chip design database |
| US6223127B1 (en) | 1997-08-15 | 2001-04-24 | Affymetrix, Inc. | Polymorphism detection utilizing clustering analysis |
| US6201639B1 (en) | 1998-03-20 | 2001-03-13 | James W. Overbeck | Wide field of view and high speed scanning microscopy |
| US6185030B1 (en) | 1998-03-20 | 2001-02-06 | James W. Overbeck | Wide field of view and high speed scanning microscopy |
| US5936324A (en) | 1998-03-30 | 1999-08-10 | Genetic Microsystems Inc. | Moving magnet scanner |
| US6185561B1 (en) | 1998-09-17 | 2001-02-06 | Affymetrix, Inc. | Method and apparatus for providing and expression data mining database |
| US6218803B1 (en) | 1999-06-04 | 2001-04-17 | Genetic Microsystems, Inc. | Position sensing with variable capacitance transducers |
| US20030097222A1 (en) | 2000-01-25 | 2003-05-22 | Craford David M. | Method, system, and computer software for providing a genomic web portal |
| US6386749B1 (en) | 2000-06-26 | 2002-05-14 | Affymetrix, Inc. | Systems and methods for heating and mixing fluids |
| US6988040B2 (en) | 2001-01-11 | 2006-01-17 | Affymetrix, Inc. | System, method, and computer software for genotyping analysis and identification of allelic imbalance |
| US20020183936A1 (en) | 2001-01-24 | 2002-12-05 | Affymetrix, Inc. | Method, system, and computer software for providing a genomic web portal |
| US20030120432A1 (en) | 2001-01-29 | 2003-06-26 | Affymetrix, Inc. | Method, system and computer software for online ordering of custom probe arrays |
| US6804679B2 (en) | 2001-03-12 | 2004-10-12 | Affymetrix, Inc. | System, method, and user interfaces for managing genomic data |
| US20030100995A1 (en) | 2001-07-16 | 2003-05-29 | Affymetrix, Inc. | Method, system and computer software for variant information via a web portal |
| US6872529B2 (en) | 2001-07-25 | 2005-03-29 | Affymetrix, Inc. | Complexity management of genomic DNA |
| US20040002818A1 (en) | 2001-12-21 | 2004-01-01 | Affymetrix, Inc. | Method, system and computer software for providing microarray probe data |
| US20030120431A1 (en) | 2001-12-21 | 2003-06-26 | Affymetrix, Inc. | Method and computer software product for genomic alignment and assessment of the transcriptome |
| JP2004003989A (ja) | 2002-03-15 | 2004-01-08 | Affymetrix Inc | 生物学的物質の走査のためのシステム、方法、および製品 |
| US20040049354A1 (en) | 2002-04-26 | 2004-03-11 | Affymetrix, Inc. | Method, system and computer software providing a genomic web portal for functional analysis of alternative splice variants |
| US20040126840A1 (en) | 2002-12-23 | 2004-07-01 | Affymetrix, Inc. | Method, system and computer software for providing genomic ontological data |
| US20050042654A1 (en) | 2003-06-27 | 2005-02-24 | Affymetrix, Inc. | Genotyping methods |
| US7317415B2 (en) | 2003-08-08 | 2008-01-08 | Affymetrix, Inc. | System, method, and product for scanning of biological materials employing dual analog integrators |
| US20100227770A1 (en) | 2005-09-13 | 2010-09-09 | Randall True | Brownian microbarcodes for bioassays |
| WO2008069906A2 (en) * | 2006-11-14 | 2008-06-12 | The Regents Of The University Of California | Digital expression of gene analysis |
| WO2009002607A1 (en) * | 2007-05-01 | 2008-12-31 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for identifying transforming and tumor suppressor genes |
| EP2173509A4 (en) | 2007-06-25 | 2013-11-06 | Affymetrix Inc | STRUCTURED MICROCODES |
| CA2939565C (en) * | 2007-10-25 | 2018-05-01 | Landmark Ip Holdings, Llc | System and method for anaerobic digestion of biomasses |
| US9434985B2 (en) * | 2008-09-25 | 2016-09-06 | University Of Massachusetts | Methods of identifying interactions between genomic loci |
| WO2015048595A1 (en) * | 2013-09-27 | 2015-04-02 | Jay Shendure | Methods and systems for large scale scaffolding of genome assemblies |
| CN103981263B (zh) | 2014-05-12 | 2016-06-29 | 浙江大学 | 土壤中抗生素抗性基因的定量检测方法 |
| CA3002740A1 (en) * | 2015-10-19 | 2017-04-27 | Dovetail Genomics, Llc | Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection |
| GB201518843D0 (en) * | 2015-10-23 | 2015-12-09 | Isis Innovation | Method of analysing DNA sequences |
-
2018
- 2018-06-26 WO PCT/US2018/039454 patent/WO2019005763A1/en not_active Ceased
- 2018-06-26 US US16/625,203 patent/US11753637B2/en active Active
- 2018-06-26 JP JP2020520434A patent/JP7277058B2/ja active Active
- 2018-06-26 CA CA3066745A patent/CA3066745A1/en active Pending
- 2018-06-26 EP EP18822702.9A patent/EP3645547A4/en active Pending
-
2023
- 2023-03-03 JP JP2023032861A patent/JP2023065620A/ja active Pending
- 2023-07-28 US US18/361,671 patent/US20240060066A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016537029A (ja) | 2013-11-18 | 2016-12-01 | エラスムス ユニベルジテート メディッシュ セントルム ロッテルダム | 三次元dna構造におけるヌクレオチド配列の相互作用を分析する方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3066745A1 (en) | 2019-01-03 |
| EP3645547A4 (en) | 2021-04-21 |
| JP2023065620A (ja) | 2023-05-12 |
| WO2019005763A1 (en) | 2019-01-03 |
| JP2020525046A (ja) | 2020-08-27 |
| EP3645547A1 (en) | 2020-05-06 |
| US11753637B2 (en) | 2023-09-12 |
| US20240060066A1 (en) | 2024-02-22 |
| US20200140943A1 (en) | 2020-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5986572B2 (ja) | 固定化プライマーを使用した標的dnaの直接的な捕捉、増幅、および配列決定 | |
| EP3050976B1 (en) | Multiplexed genomic gain and loss assays | |
| JP6803327B2 (ja) | 標的化されたシークエンシングからのデジタル測定値 | |
| JP2004524044A (ja) | 制限部位タグ付きマイクロアレイを用いたハイスループットゲノム解析方法 | |
| WO2013192292A1 (en) | Massively-parallel multiplex locus-specific nucleic acid sequence analysis | |
| US20150024948A1 (en) | Method for detecting balanced chromosomal aberrations in a genome | |
| US20240060066A1 (en) | Method for the clustering of dna sequences | |
| EP3565906B1 (en) | Quantifying dna sequences | |
| US20230374574A1 (en) | Compositions and methods for highly sensitive detection of target sequences in multiplex reactions | |
| JP2024035110A (ja) | 変異核酸の正確な並行定量するための高感度方法 | |
| CN101235418A (zh) | 基因变异检体的检测方法、专用设备及用具组 | |
| KR20240037181A (ko) | 핵산 농축 및 검출 | |
| KR20180053679A (ko) | 단일 뉴클레오타이드 해상도를 가진 다가 프로브 | |
| HK40110042A (zh) | 核酸的富集和检测 | |
| HK1148316B (en) | Multiplexed genomic gain and loss assays |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210602 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20210602 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20220418 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20220428 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20220725 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20220915 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20221027 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20221205 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230303 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20230412 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20230504 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7277058 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |