JP7270996B2 - beauty composition - Google Patents

Description

Application of Article 30, Paragraph 2 of the Patent Law Publication start date: April 24, 2015 Publication address: http://www. caa. go. jp/foods/pdf/A10-ippan. pdf http://www. caa. go. jp/foods/pdf/A10-kihon. pdf http://www. caa. go. jp/foods/pdf/A10-kinou. pdf http://www. caa. go. jp/foods/pdf/A10-anzen. pdf

TECHNICAL FIELD The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition containing ceramides and specific other ingredients as active ingredients.

The stratum corneum of the skin is present in the outermost shell of the skin and has a barrier function of the skin. The barrier function of the skin is the ability to retain moisture in the skin by suppressing transepidermal water loss and to prevent foreign substances from entering the body from the outside. Otherwise, dry skin, rough skin, dermatitis, etc. will occur. In order to exhibit this barrier function, the skin undergoes epidermal metabolism, that is, skin (epidermis) turnover.

However, when the skin is affected by external factors such as ultraviolet rays, or when aging progresses, the activity and proliferative ability of epidermal cells decrease, and accordingly the turnover rate of the skin slows down.

Therefore, improving and improving the skin barrier function is one of the important issues from the cosmetic point of view.

Examples of pharmaceuticals and cosmetics that improve and improve such skin barrier function include skin barrier function enhancers containing one or more selected from Angelica keiskei and extracts thereof (see Patent Document 1), carrots and extracts thereof. A skin barrier function enhancer containing one or more selected from (see Patent Document 2), (A) any one or more selected from nicotinamide, calcium pantothenate, and sage extract, and (B) For improving skin barrier function, comprising fatty acid menthol ester and (C) at least one selected from diisopropyl sebacate, menthoxypropanediol, and bisethoxydiglycol cyclohexane-1,4-dicarboxylate. Cosmetics (see Patent Document 3) and the like have been proposed.

JP 2015-105269 A JP 2015-105268 A JP 2013-184936 A

An object of the present invention is to provide a cosmetic composition capable of promoting skin turnover and enhancing skin barrier function by more effectively activating epidermal keratinocytes.

The present inventors have extensively investigated and researched the activation of epidermal keratinocytes in order to enhance the barrier function of the skin. The inventors have found that the effect can be obtained, and have completed the present invention. That is, the present inventors have found that epidermal keratinocytes can be synergistically activated by combining ceramides with specific other components that have little or no ability to activate epidermal keratinocytes.

That is, the present invention relates to a cosmetic composition characterized by containing ceramides and at least one component selected from the group consisting of the following (a) to (d) as active ingredients.
(a) at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose; (b) at least one amino acid selected from cysteine, leucine, lysine, methionine, and tyrosine; and (c) selected from iron and molybdenum. (d) at least one beauty ingredient selected from collagen and proteoglycan

The cosmetic composition of the present invention has a skin barrier function-improving/improving action and can be used as a skin barrier function-improving/improving agent used for improving/improving the skin barrier function.

The cosmetic composition of the present invention may be obtained by adding an active ingredient consisting of ceramides and at least one ingredient selected from the group consisting of (a) to (d).

Moreover, the cosmetic composition of the present invention is preferably for oral use, and is preferably in the form of tablets, capsules, powders, granules, or liquids.

The present invention also relates to a cosmetic method (excluding medical practice) characterized by ingesting the cosmetic composition of the present invention.

According to the cosmetic composition of the present invention, by more effectively activating epidermal keratinocytes, it is possible to promote skin turnover and more effectively enhance the barrier function of the skin.

Fig. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides + sweetener) of the present invention is applied to normal human epidermal keratinocytes (NHEK). The right figure shows the one using reduced palatinose as a sweetener. FIG. 10 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides+sweetener) of the present invention is applied to normal human epidermal keratinocytes (NHEK), and shows that reduced maltose is used as the sweetener. Fig. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides + amino acids) of the present invention is applied to normal human epidermal keratinocytes (NHEK). The right figure shows the one using leucine as an amino acid. Fig. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides + amino acids) of the present invention is applied to normal human epidermal keratinocytes (NHEK), and the left diagram shows lysine (lysine-hydrochloride) as an amino acid. The right figure shows the one using methionine as an amino acid. FIG. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides+amino acids) of the present invention is applied to normal human epidermal keratinocytes (NHEK), and shows the results using tyrosine as the amino acid. Fig. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides + minerals) of the present invention is applied to normal human epidermal keratinocytes (NHEK). The right figure shows the one using molybdenum (molybdenum chloride) as minerals. Fig. 2 is a diagram showing the cell activation activity rate when the cosmetic composition (ceramides + cosmetic ingredients) of the present invention is applied to normal human epidermal keratinocytes (NHEK). The right figure shows the one using proteoglycan as a beauty ingredient.

The cosmetic composition of the present invention contains ceramides and at least one component selected from the group consisting of the following (a) to (d) (hereinafter sometimes referred to as other components) as active ingredients. Characterized by The following components (a) to (d) used together with ceramides may be used singly (for example, component (a)), or two or more may be used in combination. When two or more other components are used in combination, it is preferable to combine ceramides with other components having a high synergistic effect.

By more effectively activating epidermal keratinocytes, the cosmetic composition of the present invention can promote skin turnover and more effectively enhance the barrier function of the skin. The cosmetic composition of the present invention has skin barrier function improving/enhancing action, skin turnover promoting action, epidermal keratinocyte activating action, and the like. Therefore, the cosmetic composition of the present invention can be used as an agent for improving/enhancing skin barrier function, an agent for promoting skin turnover, and an agent for activating epidermal keratinocytes.

[Ceramides]
Ceramides used in the present invention include human-type ceramides such as ceramide 1, ceramide 2, and ceramide 3, animal-derived ceramides extracted from the brains and spinal cords of cows, horses, pigs, etc., wheat, rice, and soybeans. plant-derived ceramides extracted from spinach, etc. can be used. In addition, the ceramides used in the present invention may be sugar ceramides, and specific examples thereof include those in which monosaccharides such as galactosylceramide and glucosylceramide are bound, and those in which oligosaccharides are bound. can.

[Other ingredients]
(a) Sweetener In the cosmetic composition of the present invention, it is preferable to use at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose as an active ingredient together with ceramides. The sweetener of the present invention means one exhibiting sweetness, and is not limited to the purpose of use as a sweetener.

(b) Amino acid In the cosmetic composition of the present invention, it is preferable to use at least one amino acid (including salts) selected from cysteine, leucine, lysine, methionine, and tyrosine as an active ingredient together with ceramides. Examples of salts include sodium salts, hydrochlorides, and the like.

(c) Minerals In the cosmetic composition of the present invention, it is preferable to use at least one mineral selected from iron and molybdenum as an active ingredient together with ceramides. Iron and molybdenum as minerals in the present invention include the forms of compounds containing iron and molybdenum, respectively.

(d) Cosmetic Ingredients In the cosmetic composition of the present invention, it is preferable to use at least one cosmetic ingredient selected from collagen and proteoglycan as an active ingredient together with ceramides.

Collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein, and telopeptide portion obtained by treating collagen molecule with protease. contains atelocollagen with the As the animal-derived collagen, fish-derived collagen is particularly preferable. Although the average molecular weight (weight average molecular weight) of collagen is not particularly limited, it is preferably from 500 to 100,000, more preferably from 1,000 to 50,000.

Proteoglycans are complex polysaccharides in which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bound to a protein core, and are abundant in animal tissues, particularly cartilage tissues. It is also known that proteoglycan exists in vivo in a structure in which the core protein is further bound to hyaluronic acid, and its molecular weight is as large as tens of thousands to tens of millions. Proteoglycans used in the present invention specifically include animal-derived proteoglycans, and commercially available products can be used.

The cosmetic composition of the present invention contains ceramides and predetermined other ingredients, and is not particularly limited as long as it can be distinguished from other products as a product in terms of being used for beauty care. , the main body of the product according to the present invention, packaging, instructions, or promotional material has the function of improving or improving skin barrier function, promoting skin turnover, or activating epidermal keratinocytes. The scope of the present invention falls within the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including quasi-drugs), cosmetics, foods for specified health uses, foods with nutrient function claims, functional foods whose efficacy has been approved by designated organizations, etc. can be mentioned. Examples of so-called health foods include those labeled as "barrier the skin", "protect the skin", "improve the barrier function of the skin", "enhance the moisturizing power of the skin", and the like. The cosmetic composition of the present invention can be ingested by a person who is concerned about the above effects regardless of gender or age. , preferably by women, more preferably by women in their 30s or later, and particularly preferably by postmenopausal women.

The cosmetic composition of the present invention can be used orally or externally. The external preparation is not particularly limited as long as it is applied to the skin, scalp, etc., and its forms include ointments, creams, gels, lotions, milky lotions, packs, poultices, and the like. Forms such as skin external preparations and injections can be mentioned.

In addition, when the cosmetic composition of the present invention is used as an oral preparation, its forms include, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, and solids. Examples include shaped agents, round agents, paste agents, cream agents, caplet agents, gel agents, chewable agents, stick agents, and the like. Among these, tablets, capsules, powders, granules and liquids are particularly preferred. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages and instant granule beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc. can be exemplified. These are preferable in that they are easy to drink with meals and can improve palatability.

The contents of ceramides and other ingredients (active ingredients) in the cosmetic composition of the present invention may be appropriately contained within the range in which the effects thereof are exhibited.

In general, when the cosmetic composition of the present invention is a drug or supplement (tablets, capsules), it preferably contains 0.01 to 100% by mass of the active ingredient in terms of dry mass. It is more preferably contained in an amount of 0.1 to 85% by mass, more preferably 0.5 to 70% by mass.

When the cosmetic composition of the present invention is a packaged beverage (liquid formulation), it preferably contains 0.01 to 15% by weight of the total active ingredient in terms of dry weight, and 0.03 to 12.5% by weight. It is more preferably contained by mass %, and more preferably contained by 0.05 to 10 mass %.

In addition, when the cosmetic composition of the present invention is an instant powdered beverage (powder) or an instant granular beverage (granules), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass of the whole. preferably 0.5 to 70% by mass, and even more preferably 1 to 60% by mass.

In order to exhibit the effect of the present invention more effectively, the active ingredient is preferably contained in an amount of 80% by mass or more (excluding water) of the entire cosmetic composition of the present invention in terms of dry mass, and 90% by mass or more. It is more preferably contained, more preferably 95% by mass or more, and particularly preferably 100% by mass. Furthermore, when the cosmetic composition of the present invention contains any one of components (a) to (d), it is preferable that the contained components consist only of the active ingredients of the present invention. That is, when the cosmetic composition of the present invention contains, for example, component (a) (sweetener), it is preferably configured so as not to contain sweeteners other than aspartame, reduced palatinose, and reduced maltose.

The amount of intake of the cosmetic composition of the present invention is not particularly limited, but from the viewpoint of exhibiting the effects of the present invention more remarkably, the daily intake of the active ingredient should be 0.5 mg/day or more. It is preferably ingested, more preferably 1 mg/day or more, and particularly preferably 1.5 mg/day or more. Although the upper limit is not particularly limited, it is, for example, 4 g/day, preferably 2 g/day.

The cosmetic composition of the present invention can be contained in one container or divided into a plurality of containers, for example 2 to 3, so that the daily intake amount is the above-mentioned intake amount for one day.

The blending mass ratio of ceramides and other components is preferably in the range of 0.5:1 to 70:1, more preferably in the range of 0.75:1 to 60:1 in terms of dry mass. It is preferably in the range of 1:1 to 60:1, particularly preferably in the range of 1:1 to 50:1. When the compounding ratio of ceramides and other components is within the above range, the effects of the present invention can be exhibited more effectively.

The cosmetic composition of the present invention can be produced by a known formulation method by adding ingredients other than active ingredients acceptable as oral or external preparations, if necessary.

Examples of the cosmetic composition as an oral preparation of the present invention include cosmetic compositions containing active ingredients and cosmetic compositions obtained by adding active ingredients to foods (cosmetic foods). , For example, foods with an increased content of the active ingredient of the present invention compared to ordinary foods (including natural foods), and foods with the active ingredient added to foods that do not normally contain the active ingredient of the present invention can be mentioned. When adding the active ingredients, each ingredient may be added separately, may be added at the same time, or may be added together with other ingredients other than the active ingredient.

Examples of the beauty food of the present invention include beverages such as carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, smoothies, and green juice; frozen desserts such as ice cream, ice sherbet, and shaved ice; Noodles such as instant noodles; Sweets such as candies, candies, gums, chocolates, tablets, snacks, biscuits, jellies, jams, creams, baked goods, and breads; Processed marine and livestock foods such as fish cakes, hams, and sausages; Dairy products such as milk, fermented milk, and yogurt; Fats and oils such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, and dressings, and their processed foods; Seasonings such as sauces and soy sauce; Congee, rice porridge, Chinese rice bowl, katsudon, tempura rice bowl, beef bowl, hayashi rice, omelet rice, oden, mapo doufu, dumplings, steamed dumplings, hamburgers, meatballs, various sauces, and retort pouch foods such as various soups.

The cosmetic method of the present invention is characterized by ingesting the above-described cosmetic composition of the present invention, but does not include medical practice. Since the effect of the cosmetic composition of the present invention can be more effectively enjoyed, it is preferable to be consumed by women, more preferably by women in their 30s or later, and particularly by women after menopause. desirable. Examples of the method of the present invention include a method of obtaining a beauty effect by providing the beauty food of the present invention in a restaurant such as a restaurant. Moreover, in esthetic salons and the like, a method of providing the beauty food of the present invention can be mentioned as one of the treatments.

EXAMPLES The present invention will be described below based on examples.
[Example 1]
1. Cell culture (1) Normal human epidermal keratinocytes (NHEK) were cultured in a dedicated medium in a 75 cm ² flask in a 37°C, 5% CO ₂ incubator.
(2) Cells suspended by trypsin treatment were seeded in each well of a 75 cm ² flask to a 96-well plate at a cell density of 1.0×10 ⁴ cells/well.
(3) Precultured for 24 hours in a 37°C, 5% CO ₂ incubator.
(4) After removing the medium from each well, 200 μL each of a test substance-containing medium adjusted to a predetermined concentration was added and cultured in a CO ₂ incubator for 48 hours.

For the combined sample of ceramides and other components, a test substance-containing medium with a double concentration was prepared, and 100 μL of each medium was added to obtain the desired concentration. In addition, the control was prepared using a 0.5% DMSO-containing medium for the test substance dissolved in DMSO, and the other test substances were prepared using a 0.25% DMSO medium. .

As for ceramides, rice-derived glucosylceramide was used. As other components used together with ceramides, substances shown in Tables 1 to 4 were used.

2. Measurement of cell activation activity (1) After culturing for 48 hours, the medium was removed from the cultured cells, washed once with 200 μL/well of PBS, and 150 μL of Cell Counting Kit-8 solution diluted 30-fold with serum-free DMEM was added. .
(2) After standing in a 37° C., 5% CO ₂ incubator for proper color development, the absorbance at 450 nm was measured.
(3) Based on the obtained data, the cell activation activity rate (% of control) was calculated with respect to the control.

% of control = (Data sample - Data blank) / (Data control - Data blank) x 100

The results are shown in Tables 1 to 4 and FIGS. 1 to 7.

Each figure represents, from the left, the results of "control (no addition)", "single addition of ceramides", "single addition of other ingredients", and "ceramides + addition of other ingredients". Also, the numerical values in the description at the bottom of the graph indicate the sample concentration. For example, "Gcel_1000" in the upper left graph of FIG. 1 indicates ceramides (glucosylceramide) 1000 (μg/mL). Concentration units for added components are μg/mL in all graphs. The vertical axis indicates "% of control".

As shown in Tables 1 to 4 and FIGS. 1 to 7, the combination of ceramides and other specific ingredients of the present invention synergistically increased epidermal keratinocyte activating activity. This indicates proliferation of epidermal keratinocytes. Therefore, according to the cosmetic composition of the present invention, it is possible to promote skin turnover and more effectively enhance the skin barrier function.

[Example 2] (Production of tablets)
A tablet (300 mg) was produced by mixing and tableting the following ingredients. Take this tablet by swallowing 2 tablets a day with water.

Weight ratio Reduced palatinose 40.0
Collagen peptide 25.0
Glucosylceramide derived from rice 3.3
Porcine placenta 14.0
Cellulose 10.0
Niacin 2.0
Calcium stearate 3.2
Silicon dioxide 2.0
Vitamin B2 0.3
Vitamin B6 0.2

[Example 3] (Production of capsules)
A mixture of the following components was encapsulated in soft capsules to produce capsules (600 mg). The capsule is to be taken one capsule daily.

Weight ratio Reduced maltose 20.0
Glucosylceramide derived from rice 4.0
Collagen peptide 25.0
Proteoglycan 25.0
Vitamin C 10.0
Cysteine 5.0
Methionine 5.0
Iron chloride 6.0

[Example 4] (Production of granules)
Granules (1200 mg) were prepared by a conventional method by mixing the following ingredients.

Weight ratio Aspartame 5.0
Rice-derived glucosylceramide 0.2
Cellulose 20.0
Calcium stearate 20.0
Collagen peptide 15.0
Proteoglycan 15.0
Vitamin C 10.0
Tyrosine 2.8
Leucine 2.0

[Example 5] (Production of instant powder)
An instant powder (5000 mg) was prepared by a conventional method by mixing the following ingredients.

Weight ratio Aspartame 5.0
Rice-derived glucosylceramide 0.05
Cellulose 20.0
Calcium stearate 20.0
Collagen peptide 15.0
Proteoglycan 15.0
Vitamin C 10.0
Vitamin B1 4.0
Vitamin B2 3.0
Vitamin E 3.0
Tyrosine 2.8
Leucine 2.0

[Example 6] (manufacture of liquid agent)
A liquid formulation (50 mL) was prepared from the following ingredients.

Weight ratio Reduced maltose 5.0
Aspartame 1.0
Glucosylceramide derived from rice 0.01
collagen peptide 7.0
Proteoglycan 5.0
Vitamin C 2.0
Vitamin B1 0.5
Vitamin B2 0.5
Vitamin E 0.2
Tyrosine 0.1
Leucine 0.1
Lysine-hydrochloride 1.0
water

Since the cosmetic composition of the present invention has a high cosmetic effect and can be used as an oral preparation or an external preparation, the industrial utility of the present invention is high.

Claims (3)

An oral cosmetic composition comprising ceramides and lysine (excluding an oral composition containing mushroom Agaricus ). 2. The oral cosmetic composition according to claim 1, which is a skin-moisturizing composition used for enhancing the moisturizing power of the skin. 3. The oral cosmetic composition according to claim 2, which is a composition for improving or enhancing skin barrier function used for improving or enhancing skin barrier function.

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