JP6906248B2 - Cosmetology composition - Google Patents

Description

The present invention relates to a cosmetological composition, and more particularly to a cosmetological composition containing a pine bark extract and a specific other ingredient as active ingredients.

In recent years, research on anti-aging to prevent skin aging such as wrinkles has been actively conducted, and collagen and hyaluronic acid are attracting attention. It is known that collagen and hyaluronic acid are produced by fibroblasts that compose the dermis of the skin.

As a composition focusing on the production of such collagen and hyaluronic acid, for example, a collagen production promoter (see Patent Document 1) characterized by having β-glucan containing β1,3D glucan, and cardamon. A beauty composition for promoting collagen production (see Patent Document 2) and a composition for promoting hyaluronic acid production (see Patent Document 3), which contains an extract extracted from a plant of the family Hamazuna. ) Has been proposed.

Japanese Unexamined Patent Publication No. 2015-71588 Japanese Unexamined Patent Publication No. 2015-48338 Japanese Unexamined Patent Publication No. 2013-155140

An object of the present invention is to provide a cosmetological composition capable of protecting the skin by reducing damage to the skin caused by ultraviolet rays or the like by protecting fibroblasts that contribute to the production of collagen and hyaluronic acid. It is in.

The present inventors have diligently investigated and studied the fibroblast protection ability of the pine bark extract, and found that a high fibroblast protection effect can be obtained by combining the pine bark extract with other specific components. We have found and completed the present invention. That is, it was found that the fibroblast protective ability can be synergistically improved by combining the pine bark extract with a specific other component having little or low fibroblast protective ability.

That is, the present invention relates to a cosmetological composition characterized by containing a pine bark extract and at least one ingredient selected from the group consisting of the following (a) to (e) as an active ingredient.
(A) At least one functional additive selected from aspartame, malt sugar, sucrose fatty acid ester, trehalose, reduced palatinose, reduced malt sugar, and calcium stearate (b) barley, sweet potato, long-lived grass, yomogi, kudzu, black ginger , And at least one plant material selected from rice (c) At least one amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine. (D) Selected from vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, sodium pantothenate, niacin, folic acid, biotin, calcium, manganese, zinc, copper, and chromium. At least one vitamin / mineral (e) At least one beauty ingredient selected from hyaluronic acid, placenta, and collagen

The cosmetological composition of the present invention has a skin protective action and can be a skin protective agent used for skin protection.

The cosmetological composition of the present invention may be obtained by adding an active ingredient consisting of a pine bark extract and at least one ingredient selected from the group consisting of (a) to (e).

The cosmetological composition of the present invention is preferably for oral use, preferably tablets, capsules, powders, granules, or liquids.

The present invention also relates to a cosmetological method (excluding medical practice) characterized in that the cosmetological composition of the present invention is ingested.

According to the cosmetological composition of the present invention, fibroblasts that contribute to the production of collagen and hyaluronic acid can be protected, and the skin can be protected from damage such as ultraviolet rays, and wrinkle prevention, anti-aging, etc. You can get the beauty effect of.

It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + plant material) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses barley as the plant material, the upper right graph uses sweet potatoes as the plant material, and the lower left graph uses long-lived grass as the plant material, and the right. The graph below shows the use of barley as a plant material. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + plant material) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses kudzu as a plant material, the upper right graph uses black ginger as a plant material, and the lower left graph uses rice (rice bran) as a plant material. be. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + functional additive) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses aspartame as a functional additive, the upper right graph uses maltose as a functional additive, and the lower left graph uses sucrose fatty acid ester as a functional additive. The graph on the lower right shows the use of trehalose as a functional additive. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + functional additive) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses reduced palatinose as a functional additive, the upper right graph uses reduced maltose as a functional additive, and the lower left graph uses reduced calcium stearate as a functional additive. Is used. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + amino acid) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses alanine as an amino acid, the upper right graph uses arginine as an amino acid, the lower left graph uses asparagine as an amino acid, and the lower right graph uses asparagine. , Aspartic acid is used as an amino acid. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + amino acid) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses cysteine as an amino acid, the upper right graph uses glycine as an amino acid, the lower left graph uses isoleucine as an amino acid, and the lower right graph uses isoleucine. , Methionine is used as an amino acid. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + amino acid) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses phenylalanine as an amino acid, the upper right graph uses proline as an amino acid, the lower left graph uses tryptophan as an amino acid, and the lower right graph uses tryptophan. , Tyrosine is used as an amino acid. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + amino acid) of this invention is applied to a normal human fibroblast (NHDF), and the thing which used valine as an amino acid. Is. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + vitamins, minerals) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses vitamin A as vitamins, the upper right graph uses vitamin B1 as vitamins, and the lower left graph uses vitamin B2 as vitamins. , The lower right graph shows the use of vitamin B6 as vitamins. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + vitamins, minerals) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses vitamin B12 as vitamins, the upper right graph uses vitamin C as vitamins, and the lower left graph uses vitamin D3 as vitamins. , The lower right graph shows the use of vitamin E as vitamins. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + vitamins, minerals) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses sodium pantothenate as vitamins, the upper right graph uses niacin as vitamins, and the lower left graph uses folic acid as vitamins. The lower right graph shows the use of biotin as a vitamin. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition (pine bark extract + vitamins, minerals) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses calcium as minerals, the upper right graph uses manganese as minerals, and the lower left graph uses zinc as minerals, lower right. The graph in is the one using copper as a mineral. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the beauty composition of this invention (pine bark extract + vitamins, minerals) is applied to normal human fibroblasts (NHDF), and chromium as a minerals Is used. It is a figure which shows the result of the cell damage suppression rate by oxidative stress when the cosmetological composition (pine bark extract + cosmetological component) of this invention is applied to normal human fibroblasts (NHDF). The upper left graph uses hyaluronic acid as a beauty ingredient, the upper right graph uses placenta as a beauty ingredient, and the lower left graph uses collagen as a beauty ingredient.

The cosmetological composition of the present invention contains a pine bark extract and at least one component (hereinafter, may be referred to as another component) selected from the group consisting of the following (a) to (e) as an active ingredient. It is characterized by that. The beauty composition of the present invention protects fibroblasts more effectively from oxidative stress by the synergistic effect of the combination of such active ingredients, suppresses a decrease in collagen and hyaluronic acid production, and damages the skin due to ultraviolet rays and the like. It can be reduced to effectively prevent or ameliorate symptoms such as wrinkles and aging.

The cosmetological composition of the present invention has a protective action on the skin (dermis) and can be a skin protective agent used for skin protection. The cosmetological composition of the present invention can also be used as a collagen production promoter or a hyaluronic acid production promoter.

[Pine bark extract]
The pine bark extract, which is the active ingredient of the cosmetological composition of the present invention, contains proanthocyanidins as one of the main ingredients. Proanthocyanidins are a group of compounds composed of flavan-3-ol and / or flavan-3,4-diol as a constituent unit and having a degree of polymerization of 2 or more.

Examples of the raw material of the pine bark extract used in the present invention include French coastal pine (Pinus Martima), Karamatsu, Black pine, Japanese red pine, Himekomatsu, Pinus parviflora, Pinus pinaster, High pine, Ryukyu pine, Utsukushi pine, Daio pine, White pine and the like. Of these, French coastal pine is preferable. The obtained extract may be concentrated or dried as required to be liquid, paste-like, or powder. As the pine bark extract, a commercially available product can be used. For example, a pine bark extract manufactured by Toyo Shinyaku Co., Ltd. can be used.

Further, the pine bark extract used in the present invention preferably contains a reduced polymer having a degree of polymerization of 2 or more as a proanthocyanidin. In particular, proanthocyanidins containing a large amount of a reduced polymer having a low degree of polymerization are preferable. Examples of the reduced polymer having a low degree of polymerization include a reduced polymer having a degree of polymerization of 2 to 30 (2 to 30 mer) and a reduced polymer having a degree of polymerization of 2 to 10 (2 to 10 mer). Preferably, a condensed polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable. In the present specification, a polymer having a degree of polymerization of 2 to 4 is referred to as OPC (oligomeric procyanidin). The pine bark extract used in the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and further preferably 40% by mass or more.

The pine bark extract is obtained by extracting the pine bark with water or an organic solvent. When water is used, hot water or hot water is preferably used. Examples of the organic solvent used for extraction include foods or chemicals such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, and hydrous propylene glycol. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more kinds of solvents. In particular, water, ethanol, hydrous ethanol and the like are preferably used.

The method for extracting the pine bark extract from the pine bark is not particularly limited, and for example, an extraction method such as a heating extraction method or a supercritical fluid extraction method can be used. In the extraction method of the pine bark extract, a plurality of extraction methods may be combined.

[Other ingredients]
(A) Functional additive In the beauty composition of the present invention, at least selected from aspartame, maltose, sucrose fatty acid ester, trehalose, reduced palatinose, reduced maltose, and calcium stearate as active ingredients together with the pine bark extract. It is preferable to use one type of functional additive. For example, aspartame, maltose, trehalose, reduced palatinose, and reduced maltose are used as sweeteners, and maltose, trehalose, reduced palatinose, and reduced maltose are also used as excipients. Further, sucrose fatty acid ester and calcium stearate are used as a lubricant and the like.

(B) Plant material In the beauty composition of the present invention, at least one plant material selected from barley, sweet potato, long-lived grass, yomogi, kudzu, black ginger, and rice is used as an active ingredient together with the pine bark extract. It is preferable to use it.

These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant materials themselves (including dried products), crushed products and juices thereof. , Extracts and other processed plant materials can be used. Examples of the pulverized product include powder, granules and the like. The juice or extract may be in liquid form, but may also be used as a paste or dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used. As these plant materials, commercially available ones can be used.

As the barley, two-row barley, six-row barley and the like can be used. As the site used as the plant material of the present invention, stems and leaves are preferable, and young leaves are particularly preferable.

Sweet potato refers to a plant belonging to the family Convolvulaceae, and is generally called sweet potato. The varieties of sweet potato are not particularly limited, and examples thereof include Satsuma mandarin, Joy White, Koganesengan, Shiroyutaka, Satsuma starch, and Ayamurasaki. As the site used as the plant material of the present invention, stems and leaves are preferable, and young leaves are particularly preferable.

Long-lived grass is a perennial plant also called Peucedanum terrestris, which belongs to the genus Peucedanum terrestris of the Umbelliferae family. Leaves and roots are preferable as the sites used as the plant material of the present invention.

Mugwort is a perennial plant of the Asteraceae family. Stems and leaves are preferable as the parts used as the plant material of the present invention.

Kuzu is a climbing perennial plant of the genus Pueraria montana in the family Leguminosae. As the site used as the plant material of the present invention, flowers and roots are preferable, and flowers are particularly preferable. As the kudzu flower, those collected in any process from the bud to the fully opened flower may be used, and those collected in each process may be mixed and used. The type of kudzu is not particularly limited, and examples thereof include Pueraria thomsonii, Pueraria lobata, and Pueraria thunbergiana.

The scientific name of Kaempferia Parviflora is Kaempferia Parviflora, and it is a plant of the genus Kaempferia of the family Zingiberaceae that grows naturally in Southeast Asia. Rhizome is preferable as the site used as the plant material of the present invention.

Rice is a plant of the genus Oryza in the family Gramineae. As the site used as the plant material of the present invention, the exodermis or embryo of brown rice is preferable, and specifically, rice bran obtained as a by-product when milling brown rice is preferably used. The type of rice is not particularly limited, and examples thereof include japonica rice and indica rice.

(C) Amino Acids In the beauty composition of the present invention, along with the pine bark extract, as active ingredients, alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine. It is preferable to use at least one amino acid (including a salt) selected from the above. Examples of the salt include sodium salts, hydrochlorides and the like.

(D) Vitamin / Minerals In the beauty composition of the present invention, along with the pine bark extract, as active ingredients, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, It is preferable to use at least one vitamin / mineral selected from sodium pantothenate, niacin, folic acid, biotin, calcium, manganese, zinc, copper, and chromium. Calcium, manganese, zinc, copper, and chromium as minerals of the present invention include the form of compounds containing these metals.

(E) Cosmetological Ingredients In the cosmetological composition of the present invention, it is preferable to use at least one cosmetological ingredient selected from hyaluronic acid, placenta, and collagen as an active ingredient together with the pine bark extract.

As the hyaluronic acid, for example, one extracted from an animal tissue such as a chicken comb or one produced by a microorganism such as a lactic acid bacterium can be used. Since the comb has been used for food for a long time and there is no problem in terms of safety, a crushed comb may be used instead of hyaluronic acid.

As the placenta, an extract of placenta derived from mammals such as humans, pigs and horses can be used. As the extraction method, a conventionally known method can be used, and the shape thereof such as liquid or powder is not particularly limited.

Collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein or collagen molecule is treated with protease to provide a telopeptide moiety. Includes atelocollagen from which. As the collagen derived from animals, collagen derived from fish is particularly preferable. The average molecular weight (weight average molecular weight) of collagen is not particularly limited, but is preferably 500 to 100,000, more preferably 1,000 to 50,000, for example.

The beauty composition of the present invention is not particularly limited as long as it contains a pine bark extract and a predetermined other component and can be distinguished from other products as a product in that it is used for beauty. For example, the scope of the present invention includes a product indicating that any of the main body, packaging, instruction manual, and advertising material of the product according to the present invention has a function of a cosmetic effect such as a skin protection effect. For example, so-called health foods such as pharmaceuticals (including non-pharmaceutical products), cosmetics, foods for specified health use, foods with nutritional function, foods with functional claims, etc. Can be mentioned. In so-called health foods, "barrier to the skin", "protect the skin", "for those who are worried about spots", "for those who are worried about wrinkles", "for skin that can withstand stress", "ultraviolet rays" It is possible to exemplify those displaying "for skin that is as good as", "happy for beauty", "happy for skin", and the like.

The cosmetological composition of the present invention can be used for oral or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp, etc., and its form is an ointment, a cream, a gel, a lotion, a milky lotion, a pack, a poultice, etc. Examples include the form of an external preparation for the skin and an injection.

When the cosmetological composition of the present invention is used as an oral preparation, the forms thereof include, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, and solids. Examples thereof include shaping agents, rounding agents, paste-like agents, cream-like agents, capsule-like agents, gel-like agents, chewable agents, stick-like agents and the like. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and instant granule beverages. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of the pine bark extract and other components (active ingredients) in the beauty composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

In general, when the beauty composition of the present invention is a pharmaceutical product or supplement (tablet, capsule), it is preferable that the active ingredient is contained in an amount of 0.01 to 100% by mass in terms of dry mass. It is more preferably 0.1 to 85% by mass, and even more preferably 0.5 to 70% by mass.

When the beauty composition of the present invention is a packaged beverage (liquid agent), it is preferable that the active ingredient is contained in an amount of 0.01 to 15% by mass in terms of dry mass, and 0.03 to 12.5. It is more preferably contained in an amount of 0.05 to 10% by mass, and further preferably contained in an amount of 0.05 to 10% by mass.

When the beauty composition of the present invention is an instant powdered beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is preferable that it is contained in an amount of 0.5 to 70% by mass, and more preferably 1 to 60% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the active ingredient is contained in an amount of 80% or more, and 90% or more, of the entire cosmetological composition (excluding water) of the present invention in terms of dry mass. It is more preferable that the content is 95% or more, and 100% is particularly preferable. Further, when the beauty composition of the present invention contains any of the components (a) to (c), it is preferable that the contained component is composed of only the active ingredient in the present invention. That is, when the beauty composition of the present invention contains, for example, component (a) (plant material), it does not contain plant materials other than barley, sweet potato, long-lived grass, mugwort, kudzu, black ginger, terminaria, rice, and coconut. It is preferable to configure it as follows.

The intake amount of the cosmetological composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the intake amount of the active ingredient per day should be 0.1 mg / day or more. It is preferably ingested, more preferably 1 mg / day or more, further preferably 10 mg / day or more, and particularly preferably 40 mg / day or more. .. The upper limit is not particularly limited, but is, for example, 4 g / day, preferably 2 g / day.

The cosmetological composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake, and can be contained as one day's worth.

The compounding mass ratio of the pine bark extract and other components is preferably in the range of 0.5: 1 to 70: 1, preferably in the range of 0.75: 1 to 60: 1 in terms of dry mass. Is more preferable, and the range of 1: 1 to 60: 1 is more preferable, and the range of 1: 1 to 50: 1 is particularly preferable. When the blending ratio of the pine bark extract and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The cosmetological composition of the present invention can be produced by a known pharmaceutical method by adding an ingredient other than the active ingredient permitted as an oral preparation or an external preparation, if necessary.

Further, as the beauty composition as an oral preparation of the present invention, in addition to beauty foods containing an active ingredient, beauty foods obtained by adding an active ingredient to a food can be mentioned, for example, usually. Examples thereof include foods in which the content of the active ingredient of the present invention is increased as compared with the food of the present invention, and foods in which the active ingredient is added to foods that do not normally contain the active ingredient of the present invention. The active ingredient may be added separately, simultaneously, or together with other ingredients other than the active ingredient.

The beauty foods of the present invention include, for example, carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, green juices and other beverages; ice cream, ice sherbets, shaved ice and other cold confectionery; buckwheat, udon, harusame, Chinese noodles, etc. , Instant noodles and other noodles; Candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread and other confectionery; Dairy products such as processed milk, fermented milk, yogurt; fats and oils such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and their processed foods; seasonings such as sauces and soy sauce; curry, stew, parent and child bowl, Examples include porridge, miscellaneous dishes, Chinese bowls, and retort pouch foods such as bowls, tempura bowls, beef bowls, hayashi rice, omelet rice, oden, marbo dough, dumplings, shumai, hamburgers, meat balls, various sauces, and various soups.

The cosmetological method of the present invention is characterized in that the cosmetological composition of the present invention described above is ingested, but does not include medical practice. Examples of the method of the present invention include a method of obtaining a beauty effect by providing the beauty food of the present invention in a restaurant such as a restaurant. Further, as one of the treatments in an aesthetic salon or the like, a method of providing the beauty food of the present invention can be mentioned.

Hereinafter, the present invention will be described based on examples.
[Example 1]
1. 1. Cell culture (1) Normal human fibroblasts (NHDF) were cultured in 10% FBS-DMEM using a 75 cm ² _{flask in a 5% CO 2 incubator at 37 ° C.}
(2) Cells suspended by trypsin treatment were seeded from a ^{75 cm 2} flask into each well of a 96-well plate at ^{a cell density of 1.0 × 10 4 cells / well.}
(3) Pre-cultured at 37 ° C. in a 5% CO ₂ incubator for 24 hours.
(4) After removing the medium from each well, 200 μL of the test substance-containing medium prepared to a predetermined concentration was added, and the cells were cultured in a _{CO 2 incubator for 24 hours.}

For the combined sample of the pine bark extract and other components, a medium containing a test substance having a double concentration was prepared, and 100 μL was added to obtain the desired concentration. Regarding control, 10% FBS-DMEM containing 0.25% DMSO was used for the test substance dissolved in DMSO, and 10% FBS-DMEM was used for other test substances. Prepared.

As the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As the other components used together with the pine bark extract, the substances shown in Tables 1 to 5 were used.

(5) After culturing for 24 hours, 50 μL of hydrogen peroxide solution prepared to 2.5 mM in serum-free DMEM was added (final concentration 500 μM). The control added with hydrogen peroxide was designated as control (+).

2. Measurement of cytostatic inhibition rate due to oxidative stress (1) 90 minutes after the addition of hydrogen peroxide solution, the medium was removed from the cultured cells, washed once with PBS 200 μL / well, and diluted 30-fold with serum-free DMEM. 150 μL of the Cell Counting Kit-8 solution was added.
(2) After allowing to stand in a 5% CO ₂ incubator at 37 ° C. to develop an appropriate color, the absorbance at 450 nm was measured.
(3) Based on the obtained data, the cell damage suppression rate (% of control) with respect to control (+) was calculated.

% of control = (Data sample-Data blank) / (Data control-Data blank) x 100

The results are shown in Tables 1 to 5 and FIGS. 1 to 14. Each figure shows the results of "control (addition of hydrogen peroxide)", "single addition of pine bark extract", "single addition of other components", and "addition of pine bark extract + other components" from the left. Further, the numerical value in the explanation at the bottom of the graph indicates the sample concentration, and for example, “pine_10” in the upper left graph of FIG. 1 indicates pine bark extract 10 (μg / mL). The concentration unit of the additive component is μg / mL in all graphs. The vertical axis indicates "% of control".

For barley, dried and crushed young leaves were used.
For sweet potatoes, dried and crushed powder of young leaves was used.
For long-lived grass, dried and crushed leaves were used.
For mugwort, finely ground powder of fresh leaves was used.
For kudzu, hot water extract of flowers (dry powder) was used.
For black ginger, a 60% ethanol extract (dry powder) of the rhizome of black ginger was used.
For rice bran, a commercially available product was used.
For maki berries, commercially available dried fruit powder was used.

As shown in Tables 1 to 5 and FIGS. 1 to 14, the combination of the pine bark extract and the specific other components of the present invention synergistically increases the suppression rate of fibroblast damage due to oxidative stress. did. Therefore, according to the cosmetological composition of the present invention, fibroblasts are protected and a high cosmetological effect can be obtained.

[Example 2] (Production of tablets)
A tablet (500 mg) composed of the following ingredients was produced.
Reduced maltose 5% by mass
Collagen peptide 15% by mass
Soy protein 15% by mass
Starch 15% by mass
Pine bark extract 8% by mass
Vitamin C 1% by mass
Vitamin B1 1% by mass
Vitamin B2 1% by mass
Vitamin B6 1% by mass
Folic acid 1% by mass
Zinc gluconate 1% by mass
Cellulose 2% by mass
Calcium chloride 2% by mass
Calcium stearate 1% by mass

[Example 3] (Manufacturing of capsules)
The following mixture was encapsulated in soft capsules to produce capsules.
Hyaluronic acid 150mg
Maltose 50 mg
Reduced maltose 50 mg
Pine bark extract 20 mg
Silicon dioxide 10 mg
Vitamin E 0.1 mg
Niacin 0.5 mg
Asparagine 0.25 mg
Aspartic acid 0.25 mg
Magnesium 1 mg

[Example 4] (Production of granules)
The following ingredients were mixed to produce granules by a conventional method.
Fructooligosaccharide 20g
Isomaltooligosaccharide 20 g
Calcium stearate 2g
Silicon dioxide 1g
Pine bark extract 0.4g
Chlorogenic acid 0.1g
Niacin 0.01g
Zinc gluconate 0.001 g

[Example 5] (Production of instant powder)
The following ingredients were mixed to produce an instant powder (10 g) by a conventional method.
Trehalose 0.1g
Aspartame 0.1g
Sucrose fatty acid ester 2g
Pine bark extract 0.4g
Alanine 0.01g
Arginine 0.02g
Glycine 0.01g
Isoleucine 0.01g
Methionine 0.01g
Phenylalanine 0.01g
Proline 0.01g
Tryptophan 0.01g
Tyrosine 0.01g
Valine 0.02g
Glutamine 0.01g
Zinc gluconate 0.001 g
Iron chloride 0.001g
Fragrance 0.001g
Calcium stearate balance

[Example 6] (Manufacturing of liquid preparation)
A liquid preparation (50 mL) consisting of the following components was produced.
Pig-derived placenta 2000mg
Pine bark extract 400 mg
Maltose 150mg
Asparagine 150 mg
Aspartic acid 150 mg
Isoleucine 50 mg
Proline 50 mg
Tryptophan 25 mg
Vitamin C 0.001% by mass
Vitamin B1 0.001% by mass
Vitamin B6 0.001% by mass
Remaining water

Since the cosmetological composition of the present invention has a high cosmetological effect and can be used as an oral preparation or an external preparation, the industrial usefulness of the present invention is high.

Claims (3)

A cosmetological composition characterized by containing a French coast pine bark extract and kudzu flower. The cosmetological composition according to claim 1, which has a protective action on the skin. The beauty composition according to claim 2, wherein the cosmetological composition is a skin protective agent used for skin protection.

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