JP7070928B2 - Cosmetology composition - Google Patents

Description

The present invention relates to a cosmetological composition, and more particularly to a cosmetological composition containing a pine bark extract and a specific other ingredient as active ingredients.

In recent years, research on anti-aging to prevent skin aging such as wrinkles has been actively conducted, and collagen and hyaluronic acid are attracting attention. It is known that collagen and hyaluronic acid are produced by fibroblasts constituting the dermis of the skin.

As a composition focusing on the production of such collagen and hyaluronic acid, for example, a collagen production promoter (see Patent Document 1) characterized by having β-glucan containing β1,3D glucan, and cardamon. A beauty composition for promoting collagen production (see Patent Document 2) and a composition for promoting hyaluronic acid production (see Patent Document 3), which comprises an extract extracted from a plant of the family Hamazuna. ) Has been proposed.

On the other hand, in modern society, blood circulation (blood flow) is often a problem due to various factors such as lack of exercise and autonomic imbalance due to stress. This disorder of blood flow (decrease in blood flow) causes symptoms such as dullness and swelling of the skin, so measures to improve blood flow are required not only from the viewpoint of health but also from the viewpoint of beauty. There is.

Examples of the composition for improving blood flow include a hisop extract (see Patent Document 4), a composition containing a cassis concentrate and an amino acid or an organic acid (see Patent Document 5), and a chamomile extract (see Patent Document 5). Patent Document 6) and the like have been proposed.

Japanese Unexamined Patent Publication No. 2015-71588 Japanese Unexamined Patent Publication No. 2015-48338 Japanese Unexamined Patent Publication No. 2013-155140 Japanese Unexamined Patent Publication No. 2006-8575 Japanese Unexamined Patent Publication No. 2004-262878 Japanese Unexamined Patent Publication No. 2005-68069

An object of the present invention is to provide a cosmetological composition having a higher skin-beautifying effect by simultaneously protecting fibroblasts and improving blood flow.

The present inventors have diligently investigated and studied beauty from both aspects of fibroblast protection and blood flow improvement, and found that the combination of pine bark extract and other specific components has a high fibroblast protection effect. We have found that a high blood flow improving effect can be obtained at the same time, and have completed the present invention. That is, by combining the pine bark extract with a specific other component having little or little ability to protect fibroblasts and / or improve blood flow, the effect of protecting fibroblasts and improving blood flow can be achieved. We have found that both can be improved synergistically.

That is, the present invention relates to a cosmetological composition comprising a pine bark extract and at least one ingredient selected from the group consisting of the following (a) to (e) as an active ingredient.
(A) At least one plant material selected from long-lived grass, black ginger, and coco palm (b) At least one sweetener selected from malt sugar, trehalose, reduced palatinose, and reduced malt sugar (c) Alanin, aspartic acid, aspartic acid At least one amino acid selected from acid, cysteine, isoleucine, methionine, phenylalanine, proline, tryptophan, and tyrosine (d) chlorogenic acid (e) at least one vitamin selected from vitamin C, niacin, folic acid, and zinc. Minerals

The beauty composition of the present invention has a skin-beautifying effect, a skin-protecting effect, and a blood flow improving effect, and can be a skin-beautifying agent used for skin beauty.

The cosmetological composition of the present invention may be obtained by adding an active ingredient consisting of a pine bark extract and at least one ingredient selected from the group consisting of (a) to (e).

In addition, the cosmetological composition of the present invention is preferably for oral use, preferably tablets, capsules, powders, granules, or liquids.

The present invention also relates to a cosmetological method (excluding medical practice) characterized in that the cosmetological composition of the present invention is ingested.

According to the cosmetological composition of the present invention, fibroblasts that contribute to the production of collagen and hyaluronic acid can be protected to protect the skin from damage such as ultraviolet rays, and wrinkle prevention and anti-aging (anti-aging) can be prevented. The beauty effect of collagen can be obtained, the blood flow can be increased, and the beauty effect such as prevention or improvement of symptoms such as dullness and swelling of the skin can be obtained. In addition, the blood flow improving action makes it possible to widely spread the fibroblast protecting action component, and it becomes possible to obtain a higher skin-beautifying effect.

The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + longevity grass) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition (pine bark extract + longevity grass) of this invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the beauty composition of the present invention (pine bark extract + black ginger) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition of this invention (pine bark extract + black ginger) to pig pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + coconut palm) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + coconut palm) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + malt sugar) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + malt sugar) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the beauty composition of the present invention (pine bark extract + trehalose) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition of the present invention (pine bark extract + trehalose) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + reduced palatinose) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition (pine bark extract + reduced palatinose) of this invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + reduced maltose) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of NO production amount at the time of applying the beauty composition of this invention (pine bark extract + reduced maltose) to pig pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + alanine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + alanine) to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + asparagine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + asparagine) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + aspartic acid) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition (pine bark extract + aspartic acid) of this invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + cysteine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetic composition (pine bark extract + cysteine) of the present invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + isoleucine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + isoleucine) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + methionine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition of the present invention (pine bark extract + methionine) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + phenylalanine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + phenylalanine) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + proline) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + proline) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + tryptophan) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + tryptophan) to porcine pulmonary arterial vascular endothelial cells (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + tyrosine) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetic composition (pine bark extract + tyrosine) of the present invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + chlorogenic acid) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition (pine bark extract + chlorogenic acid) of this invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamin C) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of cell viability. It is a figure which shows the result of the NO production amount at the time of applying the beauty composition (pine bark extract + vitamin C) of this invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + niacin) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition of the present invention (pine bark extract + niacin) to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The upper figure is a diagram showing the results of cell viability against oxidative stress when the cosmetological composition of the present invention (pine bark extract + folic acid) is applied to normal human fibroblasts (NHDF), and the lower figure is a diagram showing the results of the cell viability against oxidative stress. It is a figure which shows the result of the NO production amount at the time of applying the cosmetological composition (pine bark extract + folic acid) of the present invention to a porcine pulmonary arterial vascular endothelial cell (PPAEC). The figure above is a diagram showing the results of cell viability against oxidative stress when the cosmetic composition of the present invention (pine bark extract + zinc (zinc gluconate)) is applied to normal human fibroblasts (NHDF). The figure below shows the results of NO production when the cosmetic composition of the present invention (pine bark extract + zinc (zinc gluconate)) is applied to porcine pulmonary arterial vascular endothelial cells (PPAEC).

The cosmetological composition of the present invention contains a pine bark extract and at least one component selected from the group consisting of the following (a) to (e) (hereinafter, may be referred to as another component) as an active ingredient. It is characterized by that. That is, the following components (a) to (e) used together with the pine bark extract may be one kind (for example, the component (a)) or a combination of two or more kinds. When two or more other components are used in combination, it is preferable to combine the pine bark extract with other components having a high synergistic effect.

The beauty composition of the present invention protects fibroblasts more effectively from oxidative stress by the synergistic effect of the combination of such active ingredients, suppresses a decrease in collagen and hyaluronic acid production, and damages the skin due to ultraviolet rays and the like. It can be reduced to effectively prevent or ameliorate symptoms such as wrinkles and aging. In addition, the cosmetological composition of the present invention can more effectively relax the muscles around the blood vessels, dilate the blood vessels, and increase the blood flow due to the synergistic effect of the combination of the active ingredients, resulting in dullness and swelling of the skin. It is possible to effectively prevent or improve such symptoms. In addition, this improvement in blood flow makes it possible to spread the fibroblast protective action to the end of the body, and a higher skin-beautifying effect can be obtained.

The cosmetological composition of the present invention has a skin-beautifying effect, a skin-protecting effect, and a blood flow improving effect. The beauty composition of the present invention can be a skin-beautifying agent used for skin beauty, a skin-protecting agent used for skin protection, and a blood flow improving agent used for improving blood flow. The cosmetological composition of the present invention can also be used as a collagen production promoter or a hyaluronic acid production promoter.

[Pine bark extract]
The pine bark extract, which is the active ingredient of the cosmetological composition of the present invention, contains proanthocyanidins as one of the main ingredients. Proanthocyanidins are a group of compounds composed of flavan-3-ols and / or flavan-3,4-diols having a degree of polymerization of 2 or more as a constituent unit.

Examples of the raw material of the pine bark extract used in the present invention include French coastal pine (Pinus Martima), Japanese red pine, black pine, red pine, Japanese red pine, Japanese red pine, Japanese red pine, high pine, Ryukyu pine, Utsukushi pine, Daio pine, and white pine. Of these, French coastal pine is preferable. The obtained extract may be concentrated or dried as needed to be in the form of a liquid, paste or powder. Further, as the pine bark extract, a commercially available product can be used, and for example, a pine bark extract manufactured by Toyo Shinyaku Co., Ltd. can be used.

Further, the pine bark extract used in the present invention preferably contains a condensed polymer having a degree of polymerization of 2 or more as proanthocyanidin. In particular, proanthocyanidins containing a large amount of a condensed polymer having a low degree of polymerization are preferable. Examples of the reduced polymer having a low degree of polymerization include a condensed polymer having a degree of polymerization of 2 to 30 (2 to 30-mer) and a polymer having a degree of polymerization of 2 to 10 (2 to 10-mer). A condensed polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable, and a polymer (2 to 4) is more preferable. In the present specification, a polymer having a degree of polymerization of 2 to 4 is referred to as OPC (oligomeric protease). The pine bark extract used in the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and further preferably 40% by mass or more.

The pine bark extract is obtained by extracting the pine bark with water or an organic solvent. When water is used, hot water / hot water is preferably used. Examples of the organic solvent used for extraction include foods or chemicals such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, and hydrous propylene glycol. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more kinds of solvents. In particular, water, ethanol, hydrous ethanol and the like are preferably used.

The method for extracting the pine bark extract from the pine bark is not particularly limited, but for example, an extraction method such as a heating extraction method or a supercritical fluid extraction method can be used. In the extraction method of the pine bark extract, a plurality of extraction methods may be combined.

[Other ingredients]
(A) Plant material In the beauty composition of the present invention, it is preferable to use at least one plant material selected from long-lived grass, black ginger, and coconut as an active ingredient together with the pine bark extract.

These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant materials themselves (including dried products), their crushed products and juices. , Plant material processed products such as extracts can be used. Examples of the crushed product include powder, granules and the like. The juice or extract may be liquid, but it can also be used as a paste or a dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used. As these plant materials, commercially available ones can be used.

Long-lived grass is a perennial plant that belongs to the genus Peucedanum terrestris in the Umbelliferae family. Leaves and roots are preferable as the site used as the plant material of the present invention.

The scientific name of Kaempferia Parviflora is Kaempferia Parviflora, and it is a plant belonging to the genus Kaempferia of the family Zingiberaceae, which grows naturally in Southeast Asia. Rhizome is preferable as the site used as the plant material of the present invention.

Coconut is a plant of the family Palmaceae, and fruits (endosperm) are preferable as the site used as the plant material of the present invention.

(B) Sweetener In the beauty composition of the present invention, it is preferable to use at least one sweetener selected from maltose, trehalose, reduced palatinose, and reduced maltose as an active ingredient together with the pine bark extract. The sweetener of the present invention means a sweetener, and is not limited to the purpose of use as a sweetener.

(C) Amino acid In the beauty composition of the present invention, at least one selected from alanine, asparagine, aspartic acid, cysteine, isoleucine, methionine, phenylalanine, proline, tryptophan, and tyrosine as active ingredients together with the pine bark extract. It is preferable to use amino acids (including salts) of. Examples of the salt include sodium salts, hydrochlorides and the like.

(D) Chlorogenic acid In the beauty composition of the present invention, it is preferable to use chlorogenic acid as an active ingredient together with the pine bark extract.

(E) Vitamin / Minerals In the beauty composition of the present invention, at least one vitamin / mineral selected from vitamin C, niacin, folic acid, and zinc may be used as an active ingredient together with the pine bark extract. preferable. Zinc as a mineral of the present invention includes the form of a compound containing zinc.

The beauty composition of the present invention is not particularly limited as long as it contains a pine bark extract and a predetermined other component and can be distinguished from other products as a product in that it is used for beauty. For example, a cosmetological function such as a skin-beautifying effect, a skin-protecting effect, a blood flow improving effect, a collagen production promoting effect, and a hyaluronic acid production promoting effect in any of the main body, packaging, instruction manual, and advertising material of the product according to the present invention. The indication that there is is included in the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including quasi-drugs), cosmetics, foods for specified health use, foods with nutritional function, foods with functional claims, etc. Can be mentioned. In so-called health foods, "barrier to the skin", "protecting the skin", "for those who are worried about spots", "for those who are worried about wrinkles", "for skin that can withstand stress", "ultraviolet rays" "For skin that is as good as", "I'm happy with beauty", "I'm happy with skin", etc. It is possible to exemplify what is displayed such as "to those who become". The beauty composition of the present invention can be ingested by a person who is concerned about the above effects regardless of gender or age, but the effects of the beauty composition of the present invention can be enjoyed more effectively. , It is desirable for women to take it, and it is more desirable for postmenopausal women to take it.

The cosmetological composition of the present invention can be used for oral or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp, etc., and its form is an ointment, a cream, a gel, a lotion, a milky lotion, a pack, a poultice, etc. Examples thereof include forms such as external skin preparations and injections.

When the cosmetological composition of the present invention is used as an oral preparation, the form thereof includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, and solids. Examples thereof include shaping agents, rounding agents, paste-like agents, cream-like agents, capsule-like agents, gel-like agents, chewable agents, stick-like agents and the like. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, bottled beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and instant granule beverages. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of the pine bark extract and other components (active ingredients) in the beauty composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

In general, when the beauty composition of the present invention is a pharmaceutical product or a supplement (tablet, capsule), it is preferable that the active ingredient is contained in an amount of 0.01 to 100% by mass in terms of dry mass. It is more preferably contained in an amount of 0.1 to 85% by mass, and further preferably contained in an amount of 0.5 to 70% by mass.

When the beauty composition of the present invention is a packaged beverage (liquid), it is preferable that the active ingredient is contained in an amount of 0.01 to 15% by mass in terms of dry mass, and 0.03 to 12.5. It is more preferably contained in an amount of 0.05 to 10% by mass, and further preferably contained in an amount of 0.05 to 10% by mass.

Further, when the beauty composition of the present invention is an instant powdered beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is preferably contained in an amount of 0.5 to 70% by mass, more preferably 1 to 60% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the active ingredient is contained in an amount of 80% by mass or more, and 90% by mass or more, of the entire beauty composition of the present invention (excluding water) in terms of dry mass. It is more preferably contained, more preferably 95% by mass or more, and particularly preferably 100% by mass. Further, when the cosmetological composition of the present invention contains any of the components (a) to (c), it is preferable that the contained component is composed of only the active ingredient in the present invention. That is, when the cosmetological composition of the present invention contains, for example, the component (a) (plant material), it is preferable to configure the cosmetological composition so as not to contain any plant material other than long-lived grass, black ginger, and coconut palm.

The intake amount of the beauty composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the intake amount of the active ingredient per day should be 0.1 mg / day or more. It is preferably ingested, more preferably 1 mg / day or more, further preferably 10 mg / day or more, and particularly preferably 40 mg / day or more. .. The upper limit is not particularly limited, but is, for example, 4 g / day, preferably 2 g / day.

The cosmetological composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake, and can be contained as one day's worth.

The compounding mass ratio of the pine bark extract and other components is preferably in the range of 0.5: 1 to 70: 1, and preferably in the range of 0.75: 1 to 60: 1 in terms of dry mass. Is more preferable, and the range of 1: 1 to 60: 1 is more preferable, and the range of 1: 1 to 50: 1 is particularly preferable. When the blending ratio of the pine bark extract and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The cosmetological composition of the present invention can be produced by a known pharmaceutical method by adding an ingredient other than the active ingredient permitted as an oral preparation or an external preparation, if necessary.

Further, as the beauty composition as an oral preparation of the present invention, in addition to the beauty composition containing the active ingredient, the beauty composition (beauty food) obtained by adding the active ingredient to the food can be mentioned. For example, foods in which the active ingredient content of the present invention is increased as compared with ordinary foods (including natural foods), and foods in which the active ingredient is added to foods that do not normally contain the active ingredient of the present invention. Can be mentioned. The active ingredient may be added separately, simultaneously, or together with other ingredients other than the active ingredient.

The beauty foods of the present invention include, for example, carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, green juices and other beverages; ice cream, ice sherbets, shaved ice and other cold confectionery; soba, udon, harusame, Chinese noodles, Noodles such as instant noodles; candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread and other confectionery; Dairy products such as milk, fermented milk, yogurt; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and their processed foods; seasonings such as sauces and soy sauce; curry, stew, parent and child bowl, Examples include porridge, miscellaneous dishes, Chinese bowls, and retort pouch foods such as bowls, tempura bowls, beef bowls, hayashi rice, omelet rice, oden, marbo dough, dumplings, shumai, hamburgers, meat balls, various sauces, and various soups.

The cosmetological method of the present invention is characterized in that the cosmetological composition of the present invention described above is ingested, but does not include medical practice. The cosmetological composition of the present invention can be ingested regardless of gender or age, but since the effects of the cosmetological composition of the present invention can be more effectively enjoyed, it is desirable to ingest it to women, and it is desirable to ingest it after menopause. It is more desirable to feed it to women. Examples of the method of the present invention include a method of obtaining a cosmetological effect by providing the cosmetological food of the present invention in a restaurant such as a restaurant. Further, as one of the treatments in an esthetic salon or the like, a method of providing the beauty food of the present invention can be mentioned.

Hereinafter, the present invention will be described based on examples.
[Example 1] (Confirmation of skin protection effect)
1. 1. Cell culture (1) Normal human fibroblasts (NHDF) were cultured with 10% FBS-DMEM in a 75 cm ² flask in a 5% CO ₂ incubator at 37 ° C.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm ² flask into each well of 96 well plates at a cell density of 1.0 × 10 ⁴ cells / well.
(3) Pre-cultured at 37 ° C. in a 5% CO ₂ incubator for 24 hours.
(4) After removing the medium from each well, 200 μL of the test substance-containing medium prepared to a predetermined concentration was added, and the cells were cultured in a CO ₂ incubator for 24 hours.

For the combined sample of the pine bark extract and other components, a medium containing a test substance having a double concentration was prepared, and 100 μL was added to obtain the desired concentration. As for control, 10% FBS-DMEM containing 0.25% DMSO was used for the test substance dissolved in DMSO, and 10% FBS-DMEM was used for other test substances. Prepared.

For the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As the other components used together with the pine bark extract, the substances shown in Tables 1 to 5 were used.

(5) After culturing for 24 hours, 50 μL of hydrogen peroxide solution prepared to 2.5 mM in serum-free DMEM was added (final concentration 500 μM). Hydrogen peroxide added to Control was designated as Control (+).

2. 2. Measurement of cell viability against oxidative stress (1) 90 minutes after the addition of hydrogen peroxide solution, the medium was removed from the cultured cells, washed once with PBS 200 μL / well, and diluted 30-fold with serum-free DMEM. 150 μL of Cell Counting Kit-8 solution was added.
(2) After allowing to stand in a 5% CO ₂ incubator at 37 ° C. to develop an appropriate color, the absorbance at 450 nm was measured.
(3) Based on the obtained data, the cell viability (% of control) with respect to control (+) was calculated.

% of control = (Data sample-Data blank) / (Data control-Data blank) x 100

[Example 2] (Confirmation of blood flow improving effect)
1. 1. Cell culture
(1) Pig pulmonary artery vascular endothelial cells (PPAEC) were cultured in 10% FBS-RPMI 1640 using a 75 cm ² flask in a 5% CO ₂ incubator at 37 ° C.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm ² flask into each well of 96 well plates at a cell density of 1.0 × 10 ⁴ cells / well.
(3) Pre-cultured at 37 ° C. in a 5% CO ₂ incubator for 48 hours.
(4) After removing the medium from each well, 100 μL of the test substance-containing medium prepared to a predetermined concentration in 4% FBS-αMEM was added, and the cells were cultured in a CO ₂ incubator for 16 hours.

For the combined sample of the pine bark extract and other components, 50 μL of the test substance-containing medium having a double concentration was added to obtain the desired concentration. In addition, control was prepared using 4% FBS-αMEM containing 0.25% DMSO for the test substance dissolved in DMSO, and 4% FBS-αMEM was used for other test substances. Prepared.

For the pine bark extract, a hot water extract (dry powder) of the bark of French coastal pine was used. As the other components used together with the pine bark extract, the substances shown in Tables 1 to 5 were used.

2. 2. Evaluation of NO production
(1) After culturing for 16 hours, 50 μL of the culture supernatant was collected in a 1.5 mL sample tube, and a sample of N = 3 was mixed.
(2) Centrifuged at 4 ° C. and 2000 g for 3 minutes, and the supernatant was transferred to another 1.5 mL Eppen tube.
(3) Using Nitrate / Nitrite Assay Kit and Fluorometric (manufactured by Cayman), the NO concentration (μM) in the sample of (2) was measured. NO produced from vascular endothelial cells has the effect of relaxing the muscles around the blood vessels and expanding the blood vessels.
(4) “% of control” was calculated based on the calculated NO concentration.

% Of control
= NO concentration of sample (μM) / NO concentration of control (μM) × 100

The results of Examples 1 and 2 are shown in Tables 1 to 5 and FIGS. 1 to 22.

The upper figure of each figure shows the results of "Control (addition of hydrogen peroxide)", "Addition of pine bark extract alone", "Addition of other components alone", and "Addition of pine bark extract + other components" from the left. Represents. The lower figure of each figure shows the results of "control (without addition)", "single addition of pine bark extract", "single addition of other components", and "addition of pine bark extract + other components" from the left. Further, the numerical value in the explanation at the bottom of the graph indicates the sample concentration, and for example, “pine_10” in the graph in the upper figure of FIG. 1 indicates pine bark extract 10 (μg / mL). The concentration unit of the additive component is μg / mL in all graphs. The vertical axis shows "% of control".

For long-lived grass, dried and crushed leaves were used.
For black ginger, a 60% ethanol extract (dry powder) of the rhizome of black ginger was used.
As for coconut, commercially available coconut milk was dried and then oil-free was used.
For maki berries, commercially available dried fruit powder was used.

As shown in Tables 1 to 5 and FIGS. 1 to 22, the cell viability of fibroblasts against oxidative stress by combining the pine bark extract and the specific other components of the present invention (upper figure). And the amount of NO produced from vascular endothelial cells (see the figure below) both increased synergistically. Therefore, according to the cosmetological composition of the present invention, fibroblasts are protected and blood flow is improved, and a very excellent cosmetological effect can be obtained.

[Example 3] (Manufacturing of tablets)
A tablet (500 mg) consisting of the following ingredients was produced.
Reduced maltose 5% by mass
Collagen peptide 15% by mass
Soy protein 15% by mass
Starch 15% by mass
Pine bark extract 8% by mass
Vitamin C 1% by mass
Vitamin B1 1% by mass
Vitamin B2 1% by mass
Vitamin B6 1% by mass
Folic acid 1% by mass
Zinc gluconate 1% by mass
Cellulose 2% by mass
Calcium chloride 2% by mass
Calcium stearate 1% by mass

[Example 4] (Production of capsule)
The following mixture was encapsulated in soft capsules to produce capsules.
Hyaluronic acid 150mg
Maltose 50mg
Reduced maltose 50 mg
Pine bark extract 20 mg
Silicon dioxide 10 mg
Vitamin E 0.1 mg
Niacin 0.5mg
Asparagine 0.25 mg
Aspartic acid 0.25 mg
Magnesium 1 mg

[Example 5] (Production of granules)
The following ingredients were mixed to produce granules by a conventional method.
Fructooligosaccharide 20g
Isomaltooligosaccharide 20 g
Calcium stearate 2g
Silicon dioxide 1g
Pine bark extract 0.4g
Chlorogenic acid 0.1g
Niacin 0.01g
Zinc gluconate 0.001 g

[Example 6] (Manufacturing of instant powder)
The following components were mixed to produce an instant powder (10 g) by a conventional method.
Trehalose 0.1g
Aspartame 0.1g
Sucrose fatty acid ester 2g
Pine bark extract 0.4g
Alanine 0.01g
Arginine 0.02g
Glycine 0.01g
Isoleucine 0.01g
Methionine 0.01g
Phenylalanine 0.01g
Proline 0.01g
Tryptophan 0.01g
Tyrosine 0.01g
Valine 0.02g
Glutamine 0.01g
Zinc gluconate 0.001 g
Iron chloride 0.001g
Fragrance 0.001g
Calcium Stearate Remaining

[Example 7] (Manufacturing of liquid preparation)
A liquid preparation (50 mL) consisting of the following components was produced.
Pig-derived placenta 2000mg
Pine bark extract 400mg
Maltose 150mg
Asparagine 150mg
Aspartic acid 150mg
Isoleucine 50 mg
Proline 50mg
Tryptophan 25 mg
Vitamin C 0.001% by mass
Vitamin B1 0.001% by mass
Vitamin B6 0.001% by mass
Remaining water

Since the cosmetological composition of the present invention has a high cosmetological effect and can be used as an oral preparation or an external preparation, the industrial usefulness of the present invention is high.

Claims (1)

A composition for preventing or improving skin swelling and dullness, which is characterized by containing a pine bark extract and zinc (however, a composition for preventing or improving skin swelling and / or dullness containing collagen). Excludes) .

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