JP7071754B2 - Joint function improving composition - Google Patents

Description

The present invention relates to a composition capable of improving joint function, and more particularly to a composition containing glucosamine and a specific other ingredient as active ingredients.

As the population ages, the number of people suffering from joint pain such as knee and elbow pain is increasing, and there is a demand for medicines and the like to relieve such joint pain. As a material effective in improving arthritis, which is one of the causes of arthralgia, for example, amino sugars such as glucosamine are attracting attention (see Patent Document 1).

Japanese Patent Application Laid-Open No. 2003-325136

An object of the present invention is to provide a composition capable of protecting cartilage and improving joint function.

The present inventors have diligently investigated and studied the cartilage existing in the joint part in order to improve the joint function, and found that a high cartilage protective effect can be obtained by combining glucosamine with other specific components. , The present invention has been completed. That is, they have found that the combination of glucosamine with certain other components that have little or no cartilage protection ability can protect cartilage more effectively.

That is, the present invention relates to a composition characterized by containing glucosamine and at least one component selected from the group consisting of the following (a) to (f) as an active ingredient.
(A) At least one plant material selected from barley, long-lived grass, kudzu, rice, makiberry, and ginger (b) At least one functional additive selected from aspartame, sucrose fatty acid ester, and calcium stearate ( c) At least one amino acid selected from alanine, asparagine, glutamic acid, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine, valine, and S-adenosylmethionine (d) selected from gallic acid and chlorogenic acid. At least one polyphenol (e) At least one selected from vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D3, sodium pantothenate, niacin, biotin, vitamin K, β-carotene, calcium and selenium. Vitamins and minerals (f) At least one cartilage component selected from proteoglycan and type II collagen

The composition of the present invention can be used as a joint function improving agent having a cartilage protective action.

The composition of the present invention may be obtained by adding an active ingredient consisting of glucosamine and at least one ingredient selected from the group consisting of (a) to (f).

The composition of the present invention is preferably for oral use, preferably tablets, capsules, powders, granules, or liquids.

The present invention also relates to a method for improving joint function (excluding medical practice), which comprises ingesting the composition of the present invention.

According to the composition of the present invention, cartilage can be protected and joint function can be improved.

It is a figure which shows the expression level of MMP3 when the composition (glucosamine + plant material) of this invention is applied to a chondrocyte (NHAc-kn), and the upper left figure shows the thing which used barley as a plant material, and the upper right figure. Shows the one using long-lived grass as the plant material, the lower left figure shows the one using kudzu as the plant material, and the lower right figure shows the one using rice (rice bran) as the plant material. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + plant material) of this invention is applied to a chondrocyte (NHAc-kn), and the upper left figure shows the thing which used makiberry as a plant material, and the upper right figure. Shows the one using red ginger (Zingiber officinale var. Rubra) as the plant material, and the lower left figure shows the one using ginger (Zingiber officinale) as the plant material. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + a functional additive) is applied to a cartilage cell (NHAc-kn), and the upper left figure is a figure which used aspartame as a functional additive. The upper right figure shows the one using sucrose fatty acid ester as a functional additive, and the lower left figure shows the one using calcium stearate as a functional additive. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + amino acid) is applied to cartilage cells (NHAc-kn), the upper left figure shows the thing which used alanine as an amino acid, and the upper right figure is an amino acid. The lower left figure shows the one using glutamic acid as an amino acid, and the lower right figure shows the one using histidine as an amino acid. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + amino acid) of this invention is applied to cartilage cells (NHAc-kn), the upper left figure shows the thing which used isoleucine as an amino acid, and the upper right figure is an amino acid. The lower left figure shows the one using phenylalanine as an amino acid, and the lower right figure shows the one using proline as an amino acid. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + amino acid) of this invention is applied to a chondrocytosis (NHAc-kn), the upper left figure shows the thing which used serine as an amino acid, and the upper right figure is an amino acid. The lower left figure shows the one using valine as an amino acid, and the lower right figure shows the one using S-adenosylmethionine (SAMe) as an amino acid. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + polyphenol) of this invention is applied to a chondrocyte (NHAc-kn), the figure on the left shows the thing which used gallic acid as a polyphenol, and the figure on the right shows. The one using chlorogenic acid as a polyphenol is shown. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + vitamins, minerals) is applied to a cartilage cell (NHAc-kn), and the upper left figure shows the thing which used vitamin A as a vitamins. The upper right figure shows the one using vitamin B1 as vitamins, the lower left figure shows the one using vitamin B2 as vitamins, and the lower right figure shows the one using vitamin B12 as vitamins. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + vitamins, minerals) is applied to a cartilage cell (NHAc-kn), and the upper left figure shows the thing which used vitamin C as a vitamins. The upper right figure shows the one using vitamin D3 as a vitamin, the lower left figure shows the one using sodium pantothenate as a vitamin, and the lower right figure shows the one using niacin as a vitamin. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + vitamins, minerals) is applied to a cartilage cell (NHAc-kn), and the upper left figure shows the thing which used biotin as a vitamins. The upper right figure shows the one using vitamin K as vitamins, and the lower left figure shows the one using β-carotene as vitamins. It is a figure which shows the expression level of MMP3 when the composition of this invention (glucosamine + vitamins, minerals) is applied to cartilage cells (NHAc-kn), and the upper left figure is the one which used fossil coral Ca as a mineral. The upper right figure shows the one using shell calcined Ca as minerals, the lower left figure shows the one using shell unburned Ca as minerals, and the lower right figure shows the one using milk Ca as minerals. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + vitamin / minerals) of this invention is applied to a chondrocyte (NHAc-kn), and shows the thing which used selenium as a mineral. It is a figure which shows the expression level of MMP3 when the composition (glucosamine + cartilage component) of this invention is applied to a chondrocyte (NHAc-kn), and the left figure shows the thing which used proteoglycan as a cartilage component, and the right figure. Shows the one using type II collagen as a cartilage component.

The composition of the present invention is characterized by containing glucosamine and at least one component (hereinafter, may be referred to as another component) selected from the group consisting of the following (a) to (f) as an active ingredient. do. The following components (a) to (f) used together with glucosamine may be used alone (for example, component (a)) or in combination of two or more. When two or more other components are used in combination, it is preferable to combine glucosamine and other components having a high synergistic effect.

The composition of the present invention (joint function improving composition) can protect cartilage by suppressing the destruction of chondrocytes, and can more effectively improve (including prevention) joint pain and improve joint function. Since it can be fully exerted, it can be used as a joint function improving agent. In addition, the joint function improving composition of the present invention can protect the cartilage of the whole body and improve the pain of the joints of the whole body, but in particular, it protects the cartilage of the hip joint, the hip joint and the knee joint, and the pain in the affected portion. Can be improved, thereby improving joint function.

[Glucosamine]
As the glucosamine of the present invention, glucosamine, a salt thereof, and N-acetylglucosamine can be used. Examples of the salt include hydrochloride, sulfate, phosphate and the like. Such glucosamine may be of biological origin or may be chemically synthesized.

[Other ingredients]
(A) Plant material In the joint function improving composition of the present invention, it is preferable to use at least one plant material selected from barley, long-lived grass, kudzu, rice, makiberry, and ginger as an active ingredient together with glucosamine. ..

These plant materials may be any part of the plant such as leaves, stems, roots, flowers, fruits, trunks, branches, etc. In addition to the plant materials themselves (including dried products), crushed products and juices thereof. , Plant material processed products such as extracts can be used. Examples of the crushed product include powder, granules and the like. The squeezed juice and the extract may be liquid, but can also be used as a paste or a dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water), ethanol, or hydrous ethanol can be used. As these plant materials, commercially available ones can be used.

As the barley, two-row barley, six-row barley and the like can be used. As the site used as the plant material of the present invention, stems and leaves are preferable, and young leaves are particularly preferable.

Long-lived grass is a perennial plant that belongs to the genus Peucedanum terrestris in the Umbelliferae family. Leaves and roots are preferable as the site used as the plant material of the present invention.

Kuzu is a climbing perennial plant of the genus Pueraria montana in the family Leguminosae. As the site used as the plant material of the present invention, flowers and roots are preferable, and flowers are particularly preferable. As the kudzu flower, the one collected in any process from the bud to the fully opened flower may be used, or the one collected in each process may be mixed and used. The type of kudzu is not particularly limited, and examples thereof include Pueraria thomsonii, Pueraria lobata, and Pueraria thunbergiana.

Rice is a plant of the genus Oryza in the family Gramineae. As the site used as the plant material of the present invention, the hull or embryo of brown rice is preferable, and specifically, rice bran obtained as a by-product when milling brown rice is preferably used. The type of rice is not particularly limited, and examples thereof include japonica rice and indica rice.

Makiberry is a plant of the family Portonoki, which originates in Patagonia, Chile, and its academic name is Aristotellia chilensis. Fruits are preferable as the site used as the plant material of the present invention.

Ginger is a plant belonging to the genus Ginger of the family Zingiberaceae, and examples thereof include Sanshu ginger and Huang ginger whose scientific name is Zingiber officinale. In addition, red ginger (local name is Jahe merah) whose scientific name is Zingiber officinale var. Rubra cultivated in Indonesia and India can also be preferably used. Rhizome is preferable as the site used as the plant material of the present invention.

(B) Functional Additives In the joint function improving composition of the present invention, at least one functional additive selected from aspartame, sucrose fatty acid ester, and calcium stearate may be used as an active ingredient together with glucosamine. preferable. Aspartame can be used as a sweetener and the like, and sucrose fatty acid ester and calcium stearate can be used as a lubricant and the like.

(C) Amino Acids In the joint function improving composition of the present invention, alanine, asparagine, glutamic acid, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine, valine, and S-adenosylmethionine are used as active ingredients together with glucosamine. It is preferable to use at least one amino acid (including a salt) selected from. Examples of the salt include sodium salts, hydrochlorides and the like.
(D) Polyphenol In the joint function improving composition of the present invention, it is preferable to use at least one polyphenol selected from gallic acid and chlorogenic acid as an active ingredient together with glucosamine.

(E) Vitamin / Minerals In the joint function improving composition of the present invention, vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D3, sodium pantothenate, niacin, biotin as active ingredients together with glucosamine. , Vitamin K, β-carotene, calcium and at least one vitamin / mineral selected from selenium is preferably used. In addition, calcium and selenium as minerals of this invention include the form of the compound containing these metals.

(F) Cartilage component In the joint function improving composition of the present invention, it is preferable to use at least one cartilage component selected from proteoglycan and type II collagen as an active ingredient together with glucosamine.

Proteoglycan is a complex polysaccharide in which a protein is used as a core and glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bonded, and is abundant in animal tissues, especially cartilage tissue. It is also known that proteoglycan exists in the living body in a structure in which a core protein is further bound to hyaluronic acid, and its molecular weight is as large as tens of thousands to tens of millions. Specific examples of the proteoglycan used in the present invention include animal-derived proteoglycans, and commercially available products can be used.

Type II collagen is collagen that is abundantly contained in cartilage. For example, collagen extracted from cartilage of chicken, pig or the like can be used, and a commercially available product can be used.

The joint function improving composition of the present invention can be used as a joint function improving agent having a cartilage protective action, and the joint function improving agent contains glucosamine and other predetermined components and is used for improving joint function. In the above, there is no particular limitation as long as the product can be distinguished from other products. For example, the scope of the present invention includes a product indicating that any of the main body, packaging, instruction manual, and advertising material of the product according to the present invention has a function of improving joint function. For example, so-called health foods such as pharmaceuticals (including quasi-drugs), cosmetics, foods for specified health use, foods with nutritional function, foods with functional claims, etc. Can be mentioned. In so-called health foods, "for troubles in the knees and lower back", "for healthy walking", "for light movements", "adjusting joint function", "smoothing joint movement", "joint function" Examples of those displaying "improvement", "alleviating the trouble of joint movement", "helping joint movement", "supporting joint movement", etc. can be exemplified. The joint function improving composition of the present invention can be ingested by a person who is concerned about the above effects regardless of gender or age, but the effect of the joint function improving composition of the present invention is more effectively enjoyed. Therefore, it is desirable that it is taken by healthy people excluding the sick, and it is particularly desirable that it is taken by elderly people who have the above-mentioned problems.

The joint function improving composition of the present invention can be used for oral use or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp, etc., and its form is an ointment, a cream, a gel, a lotion, a milky lotion, a pack, a poultice, etc. Examples thereof include forms such as external skin preparations and injections.

When the joint function improving composition of the present invention is used as an oral preparation, the form thereof is, for example, a tablet, a capsule, a powder, a granule, a liquid, a granule, a rod, a plate, or a block. , Solids, rounds, pastes, creams, capsules, gels, chewables, sticks and the like. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, bottled beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and instant granule beverages. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of glucosamine and other components (active ingredients) in the joint function improving composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the joint function improving composition of the present invention is a drug or a supplement (tablet, capsule), the active ingredient is contained in an amount of 0.01 to 100% by mass in terms of dry mass. It is more preferably 0.1 to 85% by mass, and even more preferably 0.5 to 70% by mass.

When the joint function improving composition of the present invention is a packaged beverage (liquid preparation), it is preferable that the active ingredient is contained in an amount of 0.01 to 15% by mass in terms of dry mass, preferably 0.03 to 12%. It is more preferably contained in an amount of 5.5% by mass, and further preferably contained in an amount of 0.05 to 10% by mass.

Further, when the joint function improving composition of the present invention is an instant powdered beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is more preferable that it is contained in an amount of 0.5 to 70% by mass, and it is more preferable that it is contained in an amount of 1 to 60% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the active ingredient is contained in an amount of 80% by mass or more of the entire joint function improving composition (excluding water) of the present invention in terms of dry mass, preferably 90% by mass. It is more preferably contained in an amount of% or more, further preferably contained in an amount of 95% by mass or more, and particularly preferably 100% by mass. Further, when the joint function improving composition of the present invention contains any of the components (a) to (f), it is preferable that the contained component is composed of only the active ingredient in the present invention. That is, when the joint function improving composition of the present invention contains, for example, component (a) (plant material), it should be configured so as not to contain plant materials other than barley, long-lived grass, kudzu, rice, makiberry, and ginger. Is preferable.

The intake amount of the joint function improving composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the daily intake of the active ingredient should be 500 mg / day or more. It is preferably ingested, more preferably 800 mg / day or more, and even more preferably 1000 mg / day or more. The upper limit is not particularly limited, but is, for example, 5 g / day, preferably 4 g / day. The daily intake of glucosamine hydrochloride is preferably 500 to 4000 mg / day, more preferably 1000 to 3000 mg / day, and 1500 to 2500 mg / day. It is more preferable to take it so as to be 1850 to 2250 mg / day, and it is particularly preferable to take it so as to be.

The joint function improving composition of the present invention may be contained in one container or, for example, divided into a plurality of containers of 2 to 3 and contained as one day's worth so that the daily intake becomes the above-mentioned intake. can.

The compounding mass ratio of glucosamine and other components is preferably in the range of 0.5: 1 to 70: 1, and more preferably in the range of 0.75: 1 to 60: 1 in terms of dry mass. It is more preferably in the range of 1: 1 to 60: 1, and particularly preferably in the range of 1: 1 to 50: 1. When the blending ratio of glucosamine and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The joint function improving composition of the present invention can be produced by a known method by adding an ingredient other than the active ingredient permitted as an oral preparation or an external preparation, if necessary.

Further, as the joint function improving composition as an oral preparation of the present invention, in addition to the joint function improving composition containing an active ingredient, a joint function improving composition obtained by adding an active ingredient to a food (joint function). (Improved foods) can be mentioned, for example, foods having an increased active ingredient content of the present invention as compared with ordinary foods (including natural foods), and foods that do not normally contain the active ingredient of the present invention. On the other hand, foods to which an active ingredient is added can be mentioned. The active ingredient may be added separately, simultaneously, or together with other ingredients other than the active ingredient.

Examples of the joint function improving food of the present invention include beverages such as carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, and green juice; cold confectionery such as ice cream, ice sherbet, and shaved ice; buckwheat noodles, udon noodles, and Chinese noodles. Noodles such as noodles and instant noodles; Confectionery such as candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread; Dairy products such as processed milk, fermented milk, yogurt; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and their processed foods; seasonings such as sauces and soy sauce; curry, stew, parent and child bowl , Porridge, miscellaneous cooking, Chinese bowl, katsudon, tempura bowl, beef bowl, hayashi rice, omelet rice, oden, marbo dough, dumplings, shumai, hamburger, meat balls, various sauces, various soups and other retort pouch foods. ..

The method for improving joint function of the present invention is characterized by ingesting the joint function improving composition of the present invention described above, but does not include medical practice. It is desirable that the joint function improving composition of the present invention be ingested by a healthy person other than a sick person, and particularly preferably by an elderly person having the above-mentioned troubles. Examples of the method of the present invention include a method of obtaining a joint function improving effect by providing the joint function improving food of the present invention in a restaurant such as a restaurant.

Hereinafter, the present invention will be described based on examples.
[Example 1]
(1) Chondrocytes (NHAc-kn) were prepared in 10% FBS-DMEM so as to have 5 × 10 ⁴ cells / mL, and 200 μL of each was seeded into 96 well plates, and finally 1 × 10 ⁴ cells. Adjusted to / well.
(2) After pre-culturing for 1 to 2 days, samples were added as follows. The concentration of each sample was adjusted to 4 times the desired final concentration.

The sample (other components) insoluble in water was dissolved in DMSO and then adjusted to 4-fold concentration sample in 1% DMSO-10% FBS-DMEM. Ca samples other than calcium chloride were dissolved in 1N HCl and then adjusted to 4-fold sample in 1/50 N HCl-10% FBS-DMEM. The IL1b solution was adjusted at a ratio of 10 μg / mL IL1b to 2 μL with respect to 15 mL of 10% FBS-DMEM. As glucosamine, a commercially available glucosamine hydrochloride was used.

(3) Approximately 18 hours after the addition of the sample, cells were collected by CellAmp Direct RNA Prep Kit for RT-PCR (Takara). Then, the expression level of MMP3 was measured using One Step SYBR (registered trademark) PrimeScript ^TM RT-PCR Kit II. RPLP0 was also measured as an internal standard. When the MMP-3 expression level is high, it indicates that the articular chondrocyte destruction is progressing, and when the MMP-3 expression level is low, the articular chondrocyte destruction is suppressed and the articular chondrocyte is protected. It is understood that it has been done.

The results are shown in Tables 4 to 10 and FIGS. 1 to 13.

From the left, each figure shows "Control", "IL1b addition", "Glucosamine single addition (for IL1b addition)", "Glucosamine single addition (for IL1b addition)", "Glucosamine + etc. (for IL1b addition)". Represents the result of "addition of ingredients". Further, the numerical value in the explanation at the bottom of the graph indicates the sample concentration, and for example, “G_100” in the upper left graph of FIG. 1 indicates glucosamine 100 (μg / mL). The concentration unit of the additive component is μg / mL in all graphs.

For barley, dried and crushed young leaves were used.
For long-lived grass, finely ground powder of fresh leaves was used.
For kudzu, hot water extract (dry powder) of flowers was used.
For rice bran, a commercially available product was used.
For maki berries, commercially available dried fruit powder was used.
For red ginger (Zingiber officinale var. Rubra), a hot water extract (dry powder) of rhizome was used.
For ginger (Zingiber officinale), a hot water extract (dry powder) of rhizome was used.
For lemon verbena, dried and crushed leaves were used.

As shown in Tables 4 to 10 and FIGS. 1 to 13, the combination of glucosamine and a specific other component of the present invention synergistically reduced the expression level of MMP-3. Therefore, according to the joint function improving composition of the present invention, inflammation can be suppressed, articular chondrocytes can be protected (destruction of articular chondrocytes can be suppressed), and joint pain can be more effectively improved.

[Example 2] (Manufacturing of tablets)
A tablet (3000 mg with 6 tablets) consisting of the following ingredients was produced.

Glucosamine hydrochloride 2000mg
Proteoglycan 5 mg
Fossil coral Ca 150mg
Calcium stearate 50 mg
β-carotene 10 mg
Vitamin B1 5 mg
Vitamin B2 5 mg
Vitamin B12 5 mg
Vitamin D3 2.5 mg
Long-lived grass powder 300 mg
Proline 30 mg
Crystalline cellulose 50 mg
Sucrose fatty acid ester 30 mg
Maltose balance

The above tablets are divided into 6 tablets 2 to 3 times a day and taken by chewing or without chewing with water.

[Example 3] (Production of capsule)
The following mixture was encapsulated in soft capsules to produce 6 capsules.

Glucosamine hydrochloride 1500mg
Proteoglycan 5 mg
Rice bran extract 20mg
Makiberry powder 100mg
Calcined shell Ca 120mg
Vitamin E 30 mg
Niacin 5 mg
Chlorogenic acid 10 mg
Proline 20 mg
Selenium 5 mg
Natriu pantothenate 10 mg
Maltose balance

The above tablets are taken in 6 tablets 2 to 3 times a day with water.

[Example 4] (Production of granules)
The following components were mixed to prepare granules (3000 mg) by a conventional method.

Glucosamine hydrochloride 1800mg
Proteoglycan 5 mg
Seashell unbaked Ca 200mg
Sucrose fatty acid ester 50 mg
Selenium 5 mg
Vitamin E 50 mg
Barley young leaf powder 800 mg
Niacin 10 mg
Biotin 10 mg
Proline 5 mg
Starch residue

[Example 5] (Manufacturing of instant powder)
The following components were mixed to produce an instant powder (6 g) by a conventional method.

Glucosamine hydrochloride 2500mg
Proteoglycan 5 mg
Seashell unbaked Ca 200mg
Barley young leaf powder 1000 mg
Long-lived grass powder 500 mg
Ginger powder 250mg
Selenium 5 mg
Vitamin E 300mg
Vitamin K 200mg
Niacin 50mg
Biotin 50 mg
Proline 30 mg
Valine 30 mg
Leucine 20 mg
Isoleucine 20 mg
Aspartame 300mg
Oligosaccharide 500mg
Dextrin rest

[Example 6] (Manufacturing of liquid preparation)
A liquid preparation (50 mL) consisting of the following components was produced.

Glucosamine hydrochloride 2000mg
Type II collagen 25 mg
Milk Ca 200mg
Aspartame 800mg
Red ginger extract 300mg
Ginger extract 200 mg
Kuzuhana extract 150mg
Proline 100 mg
Asparagine 50mg
Valine 25 mg
Leucine 20 mg
Isoleucine 20 mg
Gallic acid 10 mg
Vitamin C 5 mg
Fragrance Appropriate amount of water balance

Since the composition of the present invention has a high joint function improving effect and can be used as an oral preparation or an external preparation, the industrial usefulness of the present invention is high.

Claims (2)

An oral composition for improving joint function , which comprises glucosamine hydrochloride and alanine as active ingredients. An oral composition for smoothing knee movement, which comprises glucosamine hydrochloride and alanine as active ingredients .

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