JP6858986B2 - Cosmetology composition - Google Patents

Description

The present invention relates to a cosmetological composition, and more particularly to a cosmetological composition containing ceramides and specific other ingredients as active ingredients.

The stratum corneum of the skin is located in the outermost shell of the skin and serves as a barrier function of the skin. The skin barrier function is a function that retains skin moisture by suppressing the amount of evapotranspiration through the skin and prevents foreign substances from entering the living body from the outside, and this skin barrier function is fully exerted. Otherwise, dry skin, rough skin, dermatitis, etc. will occur. In the skin, in order to exert this barrier function, the metabolism of the epidermis, that is, the turnover of the skin (epidermis) is performed.

However, when the skin is affected by external factors such as ultraviolet rays or ages, the activity and proliferative ability of epidermal cells decrease, and the turnover rate of the skin is delayed accordingly.

Therefore, improving and improving the skin barrier function is one of the important issues from a cosmetic point of view.

As pharmaceuticals and cosmetics for improving / improving such skin barrier function, for example, a skin barrier function enhancer containing at least one selected from touki and its extract (see Patent Document 1), carrot and its extract. A skin barrier function enhancer containing one or more selected from (see Patent Document 2), one or more selected from (A) nicotinic acid amide, calcium pantothenate, and sage extract, and (B). For improving skin barrier function, which is a combination of a fatty acid menthol ester and one or more selected from (C) diisopropyl sebacate, mentoxypropanediol, and bisethoxydiglycolcyclohexane-1,4-dicarboxylic acid. Cosmetics (see Patent Document 3) and the like have been proposed.

Japanese Unexamined Patent Publication No. 2015-105269 Japanese Unexamined Patent Publication No. 2015-105268 Japanese Unexamined Patent Publication No. 2013-184936

An object of the present invention is to provide a cosmetological composition capable of promoting skin turnover and enhancing the barrier function of the skin by activating epidermal keratinocytes more effectively.

The present inventors have diligently investigated and studied the activation of epidermal keratinocytes in order to enhance the barrier function of the skin. As a result, the combination of ceramides and specific other components results in high activation of epidermal keratinocytes. We have found that an effect can be obtained, and have completed the present invention. That is, it was found that epidermal keratinocytes can be synergistically activated by combining ceramides with specific other components having little or little ability to activate epidermal keratinocytes.

That is, the present invention relates to a cosmetological composition characterized by containing ceramides and at least one ingredient selected from the group consisting of the following (a) to (d) as an active ingredient.
(A) At least one sweetener selected from aspartame, reduced palatinose, and reduced maltose (b) At least one amino acid selected from cysteine, leucine, lysine, methionine, and tyrosine (c) Selected from iron and molybdenum At least one kind of minerals (d) At least one kind of beauty ingredient selected from collagen and proteoglycan

The cosmetological composition of the present invention has a skin barrier function improving / improving effect, and can be a skin barrier function improving / improving agent used for improving / improving the skin barrier function.

The cosmetological composition of the present invention may be obtained by adding an active ingredient consisting of ceramides and at least one ingredient selected from the group consisting of (a) to (d).

The cosmetological composition of the present invention is preferably for oral use, preferably tablets, capsules, powders, granules, or liquids.

The present invention also relates to a cosmetological method (excluding medical practice) characterized in that the cosmetological composition of the present invention is ingested.

According to the cosmetological composition of the present invention, by activating epidermal keratinocytes more effectively, skin turnover can be promoted and the barrier function of the skin can be enhanced more effectively.

It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + sweetener) of this invention is applied to a normal human epidermal keratinocyte (NHEK), and the figure on the left shows the thing which used aspartame as a sweetener. The figure on the right shows the one using reduced palatinose as a sweetener. It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + sweetener) of this invention is applied to a normal human epidermal keratinocyte (NHEK), and shows the thing which used reduced maltose as a sweetener. It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + amino acid) of this invention is applied to a normal human epidermal keratinocyte (NHEK), and the figure on the left shows the thing which used cysteine as an amino acid. The figure on the right shows the one using leucine as an amino acid. It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + amino acid) of this invention is applied to a normal human epidermal keratinocyte (NHEK), and the figure on the left shows lysine (lysine-hydrochloride) as an amino acid. The one used is shown, and the figure on the right shows the one using methionine as an amino acid. It is a figure which shows the cell activation activity rate at the time of applying the cosmetological composition (ceramides + amino acid) of this invention to a normal human epidermal keratinocyte (NHEK), and shows the thing which used tyrosine as an amino acid. It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + minerals) of this invention is applied to normal human epidermal keratinocytes (NHEK), and the figure on the left shows iron (iron chloride) as a mineral. The one used is shown, and the figure on the right shows the one using molybdenum (molybdenum chloride) as a mineral. It is a figure which shows the cell activation activity rate when the beauty composition (ceramides + beauty component) of this invention is applied to a normal human epidermal keratinocyte (NHEK), and the figure on the left shows the thing which used collagen as a beauty component. The figure on the right shows the one using proteoglycan as a beauty ingredient.

The cosmetological composition of the present invention contains ceramides and at least one component (hereinafter, may be referred to as another component) selected from the group consisting of the following (a) to (d) as an active ingredient. It is a feature. The following components (a) to (d) used together with ceramides may be used alone (for example, component (a)) or in combination of two or more. When two or more other components are used in combination, it is preferable to combine ceramides and other components having a high synergistic effect.

The cosmetological composition of the present invention can promote skin turnover and more effectively enhance the barrier function of the skin by activating epidermal keratinocytes more effectively. The cosmetological composition of the present invention has a skin barrier function improving / improving action, a skin turnover promoting action, an epidermal keratinocyte activating action, and the like. Therefore, the cosmetological composition of the present invention can be used as a skin barrier function improving / improving agent, a skin turnover promoter, and an epidermal keratinocyte activator.

[Ceramides]
The ceramides used in the present invention include human ceramides such as ceramide 1, ceramide 2, and ceramide 3, as well as animal-derived ceramides extracted from the brain and spinal cord of cows, horses, pigs, etc., wheat, rice, and soybeans. , Plant-derived ceramide extracted from spinal cord and the like can be used. Further, the ceramides used in the present invention may be sugar ceramides, and specific examples thereof include those to which monosaccharides such as galactosylceramide and glucosylceramide are bound, and those to which oligosaccharides are bound. it can.

[Other ingredients]
(A) Sweetener In the beauty composition of the present invention, it is preferable to use at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose as an active ingredient together with ceramides. The sweetener of the present invention means one that exhibits sweetness, and is not limited to the purpose of use as a sweetener.

(B) Amino Acids In the beauty composition of the present invention, it is preferable to use at least one amino acid (including a salt) selected from cysteine, leucine, lysine, methionine, and tyrosine as an active ingredient together with ceramides. Examples of the salt include sodium salts, hydrochlorides and the like.

(C) Minerals In the beauty composition of the present invention, it is preferable to use at least one mineral selected from iron and molybdenum as an active ingredient together with ceramides. In addition, iron and molybdenum as minerals of this invention include the form of the compound containing iron and molybdenum, respectively.

(D) Cosmetological Ingredients In the cosmetological composition of the present invention, it is preferable to use at least one cosmetological ingredient selected from collagen and proteoglycan as an active ingredient together with ceramides.

Collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein or collagen molecule is treated with protease to provide a telopeptide moiety. Includes atelocollagen from which. As the collagen derived from animals, collagen derived from fish is particularly preferable. The average molecular weight (weight average molecular weight) of collagen is not particularly limited, but is preferably 500 to 100,000, more preferably 1,000 to 50,000, for example.

Proteoglycan is a complex polysaccharide in which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bonded to a protein as a core, and is abundantly present in animal tissues, especially cartilage tissues. It is also known that proteoglycan exists in the living body in a structure in which a core protein is further bound to hyaluronic acid, and its molecular weight is as large as tens of thousands to tens of millions. Specific examples of the proteoglycan used in the present invention include animal-derived proteoglycans, and commercially available products can be used.

The beauty composition of the present invention is not particularly limited as long as it contains ceramides and other predetermined components and can be distinguished from other products as a product in that it is used for beauty, for example. , The main body of the product, the packaging, the instruction manual, or the advertisement according to the present invention has a cosmetic effect such as a skin barrier function improving / improving effect, a skin turnover promoting effect, and an epidermal keratinocyte activating effect. The indication to that effect is included in the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including non-pharmaceutical products), cosmetics, foods for specified health use, foods with nutritional function, foods with functional claims, etc. Can be mentioned. Examples of so-called health foods include those displaying "barrier to the skin", "protecting the skin", "enhancing the barrier function of the skin", "increasing the moisturizing power of the skin" and the like. The beauty composition of the present invention can be ingested by a person who is concerned about the above effects regardless of gender or age, but the effects of the beauty composition of the present invention can be enjoyed more effectively. , It is desirable for women to take it, it is more desirable for women in their thirties and beyond, and it is especially desirable for postmenopausal women to take it.

The cosmetological composition of the present invention can be used for oral use or external use. The external preparation is not particularly limited as long as it is applied to the skin, scalp, etc., and its form is an ointment, a cream, a gel, a lotion, a milky lotion, a pack, a poultice, etc. Examples include the form of an external preparation for the skin and an injection.

When the cosmetological composition of the present invention is used as an oral preparation, the forms thereof include, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, and solids. Examples thereof include shaping agents, rounding agents, paste-like agents, cream-like agents, capsule-like agents, gel-like agents, chewable-like agents, stick-like agents and the like. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and instant granule beverages. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of the ceramides and other components (active ingredients) in the beauty composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

In general, when the beauty composition of the present invention is a pharmaceutical product or supplement (tablet, capsule), it is preferable that the active ingredient is contained in an amount of 0.01 to 100% by mass in terms of dry mass. It is more preferably 0.1 to 85% by mass, and even more preferably 0.5 to 70% by mass.

When the cosmetological composition of the present invention is a packaged beverage (liquid preparation), it is preferable that the active ingredient is contained in an amount of 0.01 to 15% by mass in terms of dry mass, and 0.03 to 12.5. It is more preferably contained in an amount of 0.05 to 10% by mass, and further preferably contained in an amount of 0.05 to 10% by mass.

When the beauty composition of the present invention is an instant powdered beverage (powder) or an instant granule beverage (granule), the active ingredient is contained in an amount of 0.1 to 80% by mass in terms of dry mass. It is preferable that it is contained in an amount of 0.5 to 70% by mass, and more preferably 1 to 60% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the active ingredient is contained in an amount of 80% by mass or more, and 90% by mass or more, of the entire cosmetological composition of the present invention (excluding water) in terms of dry mass. It is more preferably contained, more preferably 95% by mass or more, and particularly preferably 100% by mass. Further, when the cosmetological composition of the present invention contains any of the components (a) to (d), it is preferable that the contained component is composed of only the active ingredient in the present invention. That is, when the cosmetological composition of the present invention contains, for example, the component (a) (sweetener), it is preferable that the cosmetological composition does not contain a sweetener other than aspartame, reduced palatinose, and reduced maltose.

The intake amount of the cosmetological composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the intake amount of the active ingredient per day should be 0.5 mg / day or more. It is preferably taken, more preferably 1 mg / day or more, and particularly preferably 1.5 mg / day or more. The upper limit is not particularly limited, but is, for example, 4 g / day, preferably 2 g / day.

The cosmetological composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake, and can be contained as one day's worth.

The compounding mass ratio of the ceramides and other components is preferably in the range of 0.5: 1 to 70: 1, and more preferably in the range of 0.75: 1 to 60: 1 in terms of dry mass. It is more preferably in the range of 1: 1 to 60: 1, and particularly preferably in the range of 1: 1 to 50: 1. When the blending ratio of the ceramides and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The cosmetological composition of the present invention can be produced by a known formulation method by adding an ingredient other than the active ingredient permitted as an oral preparation or an external preparation, if necessary.

Further, as the beauty composition as an oral preparation of the present invention, in addition to the beauty composition containing the active ingredient, the beauty composition (beauty food) obtained by adding the active ingredient to the food can be mentioned. For example, foods in which the active ingredient content of the present invention is increased as compared with ordinary foods (including natural foods), and foods in which the active ingredient is added to foods that do not normally contain the active ingredient of the present invention. Can be mentioned. The active ingredient may be added separately, simultaneously, or together with other ingredients other than the active ingredient.

The beauty foods of the present invention include, for example, carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, green juice and other beverages; ice cream, ice sherbets, shaved ice and other cold confectionery; buckwheat, udon, harusame, Chinese noodles, etc. Noodles such as instant noodles; Confectionery such as candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread; Dairy products such as milk, fermented milk and yogurt; fats and oils such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream and dressing and their processed foods; seasonings such as sauces and soy sauce; Examples include porridge, miscellaneous dishes, Chinese bowls, and retort pouch foods such as bowls, tempura bowls, beef bowls, hayashi rice, omelet rice, oden, marbo dough, dumplings, shumai, hamburgers, meat balls, various sauces, and various soups.

The cosmetological method of the present invention is characterized in that the cosmetological composition of the present invention described above is ingested, but does not include medical practice. Since the effects of the cosmetological composition of the present invention can be enjoyed more effectively, it is desirable to ingest it to women, more preferably to women in their thirties or older, and particularly to ingest it to postmenopausal women. desirable. Examples of the method of the present invention include a method of obtaining a beauty effect by providing the beauty food of the present invention in a restaurant such as a restaurant. Further, as one of the treatments in an aesthetic salon or the like, a method of providing the beauty food of the present invention can be mentioned.

Hereinafter, the present invention will be described based on examples.
[Example 1]
1. 1. Cell culture (1) Normal human epidermal keratinocytes (NHEK) were cultured in a special medium in _{a 5% CO 2} incubator at ^{37 ° C. using a 75 cm 2 flask.}
(2) Cells suspended by trypsin treatment were seeded from a ^{75 cm 2} flask into each well of a 96-well plate at ^{a cell density of 1.0 × 10 4 cells / well.}
(3) Pre-cultured at 37 ° C. in a 5% CO ₂ incubator for 24 hours.
(4) After removing the medium from each well, 200 μL of the test substance-containing medium prepared to a predetermined concentration was added, and the cells were cultured in a _{CO 2 incubator for 48 hours.}

For the combined sample of ceramides and other components, a medium containing a test substance having a double concentration was prepared, and 100 μL of each was added to obtain the desired concentration. The control was prepared by dissolving the test substance in DMSO using a medium containing 0.5% DMSO, and preparing the other test substances using a 0.25% DMSO medium. ..

As for ceramides, rice-derived glucosylceramide was used. As the other components used together with the ceramides, the substances shown in Tables 1 to 4 were used.

2. Cell activation activity measurement (1) After culturing for 48 hours, the medium was removed from the cultured cells, washed once with PBS 200 μL / well, and 150 μL of Cell Counting Kit-8 solution diluted 30-fold with serum-free DMEM was added. ..
(2) After allowing to stand in a 5% CO ₂ incubator at 37 ° C. to develop an appropriate color, the absorbance at 450 nm was measured.
(3) Based on the obtained data, the cell activation activity rate (% of control) with respect to control was calculated.

% of control = (Data sample-Data blank) / (Data control-Data blank) × 100

The results are shown in Tables 1 to 4 and FIGS. 1 to 7.

Each figure shows the results of "control (without addition)", "single addition of ceramides", "single addition of other components", and "addition of ceramides + other components" from the left. Further, the numerical value in the explanation at the bottom of the graph indicates the sample concentration, and for example, “Gcel_1000” in the upper left graph of FIG. 1 indicates ceramides (glucosylceramide) 1000 (μg / mL). The concentration unit of the additive component is μg / mL in all graphs. The vertical axis indicates "% of control".

As shown in Tables 1 to 4 and FIGS. 1 to 7, the combination of ceramides and the specific other components of the present invention synergistically increased the epidermal keratinocyte activation activity. This indicates that epidermal keratinocytes have proliferated. Therefore, according to the cosmetological composition of the present invention, skin turnover can be promoted and the barrier function of the skin can be enhanced more effectively.

[Example 2] (Production of tablets)
The following ingredients were mixed and tableted to produce a tablet (300 mg). Take this tablet by swallowing 2 tablets a day with water.

Weight ratio Reduced palatinose 40.0
Collagen peptide 25.0
Rice-derived glucosylceramide 3.3
Pig Placenta 14.0
Cellulose 10.0
Niacin 2.0
Calcium Stearate 3.2
Silicon dioxide 2.0
Vitamin B2 0.3
Vitamin B6 0.2

[Example 3] (Manufacturing of capsules)
A mixture of the following components was encapsulated in soft capsules to produce a capsule (600 mg). This capsule is taken 1 capsule a day.

Weight ratio Reduced maltose 20.0
Rice-derived glucosylceramide 4.0
Collagen peptide 25.0
Proteoglycan 25.0
Vitamin C 10.0
Cysteine 5.0
Methionine 5.0
Iron chloride 6.0

[Example 4] (Production of granules)
The following ingredients were mixed to prepare granules (1200 mg) by a conventional method.

Weight ratio Aspartame 5.0
Rice-derived glucosylceramide 0.2
Cellulose 20.0
Calcium Stearate 20.0
Collagen peptide 15.0
Proteoglycan 15.0
Vitamin C 10.0
Tyrosine 2.8
Leucine 2.0

[Example 5] (Production of instant powder)
The following ingredients were mixed to produce an instant powder (5000 mg) by a conventional method.

Weight ratio Aspartame 5.0
Rice-derived glucosylceramide 0.05
Cellulose 20.0
Calcium Stearate 20.0
Collagen peptide 15.0
Proteoglycan 15.0
Vitamin C 10.0
Vitamin B1 4.0
Vitamin B2 3.0
Vitamin E 3.0
Tyrosine 2.8
Leucine 2.0

[Example 6] (Manufacturing of liquid preparation)
A liquid preparation (50 mL) consisting of the following components was produced.

Weight ratio Reduced maltose 5.0
Aspartame 1.0
Rice-derived glucosylceramide 0.01
Collagen peptide 7.0
Proteoglycan 5.0
Vitamin C 2.0
Vitamin B1 0.5
Vitamin B2 0.5
Vitamin E 0.2
Tyrosine 0.1
Leucine 0.1
Lysine-hydrochloride 1.0
Water balance

Since the cosmetological composition of the present invention has a high cosmetological effect and can be used as an oral preparation or an external preparation, the industrial usefulness of the present invention is high.

Claims (2)

A beauty composition characterized by containing rice-derived glucosylceramide and aspartame (however, a composition containing γ-aminobutyric acid , collagen, tea polyphenol, glutathione, metallic sulfur protein, lycopene, vitamin A, vitamin). Excluding sunscreen beverages containing E, Vitamin C, Vitamin B2, and Vitamin B6 ). The beauty composition according to claim 1, wherein the composition is a skin moisturizing composition used to enhance the moisturizing power of the skin.

Images