JP7270992B2 - 分析物の濃縮 - Google Patents

分析物の濃縮 Download PDF

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Publication number
JP7270992B2
JP7270992B2 JP2020532561A JP2020532561A JP7270992B2 JP 7270992 B2 JP7270992 B2 JP 7270992B2 JP 2020532561 A JP2020532561 A JP 2020532561A JP 2020532561 A JP2020532561 A JP 2020532561A JP 7270992 B2 JP7270992 B2 JP 7270992B2
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Japan
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analyte
atps
minutes
phase
analytes
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Japanese (ja)
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JP2021506253A (ja
JP2021506253A5 (https=
Inventor
エドモンド ジョンソン－バック，アレキサンダー
テワリ，マニーシュ
ジー． ウォルター，ニルス
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University of Michigan System
University of Michigan Ann Arbor
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University of Michigan System
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2020532561A 2017-12-14 2018-12-13 分析物の濃縮 Active JP7270992B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762598802P 2017-12-14 2017-12-14
US62/598,802 2017-12-14
US201862692001P 2018-06-29 2018-06-29
US62/692,001 2018-06-29
PCT/US2018/065421 WO2019118705A1 (en) 2017-12-14 2018-12-13 Concentration of analytes

Publications (3)

Publication Number Publication Date
JP2021506253A JP2021506253A (ja) 2021-02-22
JP2021506253A5 JP2021506253A5 (https=) 2021-12-23
JP7270992B2 true JP7270992B2 (ja) 2023-05-11

Family

ID=66815084

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020532561A Active JP7270992B2 (ja) 2017-12-14 2018-12-13 分析物の濃縮

Country Status (5)

Country Link
US (1) US20190187031A1 (https=)
EP (1) EP3724317A4 (https=)
JP (1) JP7270992B2 (https=)
CN (1) CN111971380A (https=)
WO (1) WO2019118705A1 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12065698B2 (en) * 2009-10-02 2024-08-20 Thermo Fisher Scientific Baltics Uab Sample processing apparatus and method
JP2019505884A (ja) * 2015-12-10 2019-02-28 キアゲン ゲーエムベーハー デジタル画像内の少なくとも１つのオブジェクトの全体的明るさを判定するための方法
AU2018231211A1 (en) 2017-03-08 2019-10-03 The Regents Of The University Of Michigan Analyte detection
WO2018183465A1 (en) 2017-03-28 2018-10-04 Chiu Yin To Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
EP4303311A3 (en) 2017-09-18 2024-04-03 Phase Scientific International, Ltd. Method for using aqueous two-phase system for the isolation, purification and/or concentration of short nucleic acid fragments
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
WO2020023503A1 (en) * 2018-07-24 2020-01-30 The Regents Of The University Of Michigan Intramolecular kinetic probes
KR20250047414A (ko) * 2019-07-16 2025-04-03 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
WO2021154935A1 (en) 2020-01-29 2021-08-05 The Regents Of The University Of Michigan Analyte detection
WO2021188308A1 (en) * 2020-03-19 2021-09-23 The Regents Of The University Of Michigan Analyte detection
EP4121530A1 (en) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction
US20240140981A1 (en) * 2020-12-29 2024-05-02 Curative Inc. Nucleic acid purification methods using a water-immiscible solvent wash
JP2024513919A (ja) * 2021-04-06 2024-03-27 エックスゲノムズ コーポレーション 核酸のエピジェネティックな修飾を検出するためのシステム、方法、及び組成物
CN113324619B (zh) * 2021-06-02 2023-06-06 成都瀚辰光翼科技有限责任公司 一种基于电容检测的液面探测方法及装置
CN118414544A (zh) 2021-12-21 2024-07-30 生物辐射实验室股份有限公司 通过测量局部荧光比率在高分辨率装置中进行多重检测
WO2024064787A2 (en) * 2022-09-21 2024-03-28 Ultragenyx Pharmaceutical Inc. Method for determination of double-stranded rna impurity
CN119137270A (zh) 2022-11-02 2024-12-13 相达生物科技国际有限公司 使用atps和固相介质从生物样品分离目标分析物的方法
WO2024093522A1 (en) * 2022-11-02 2024-05-10 Phase Scientific International, Ltd. Methods, compositions and kits for concentrating target analytes from a bulk fluid sample
AU2023374887A1 (en) * 2022-11-02 2025-05-22 Phase Scientific International, Ltd. Methods and kits for isolating target nucleic acids below a target size from a sample
CN116103375B (zh) * 2022-11-11 2025-11-14 北京理工大学 基于dna折纸的纳米标尺及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011116256A2 (en) 2010-03-18 2011-09-22 The Regents Of The University Of Michigan Multiphase microarrays and uses thereof
WO2017041030A1 (en) 2015-09-04 2017-03-09 The Regents Of The University Of California Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
JP2017513015A (ja) 2014-03-07 2017-05-25 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 被分析物の抽出、濃縮及び検出を一体化するためのデバイス
WO2017139354A1 (en) 2016-02-10 2017-08-17 The Regents Of The University Of Michigan Detection of nucleic acids

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020081629A1 (en) * 2000-06-30 2002-06-27 Tibotec N.V. Apparatus for the simultaneous transfer of liquid analytes
US20060094033A1 (en) * 2004-05-21 2006-05-04 Carl Abulencia Screening methods and libraries of trace amounts of DNA from uncultivated microorganisms
EP2350113A4 (en) * 2008-11-25 2013-01-02 Ge Healthcare Bio Sciences Ab FOR AN AQUEOUS TWO-PHASE EXTRACTION REINFORCED DEPOSITION PROCESS FOR CLEANING THERAPEUTIC PROTEINS
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011116256A2 (en) 2010-03-18 2011-09-22 The Regents Of The University Of Michigan Multiphase microarrays and uses thereof
JP2017513015A (ja) 2014-03-07 2017-05-25 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 被分析物の抽出、濃縮及び検出を一体化するためのデバイス
WO2017041030A1 (en) 2015-09-04 2017-03-09 The Regents Of The University Of California Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
WO2017139354A1 (en) 2016-02-10 2017-08-17 The Regents Of The University Of Michigan Detection of nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Azevedo AM et al，Partitioning of human antibodies in polyethylene glycol-sodium citrate aqueous two-phase systems，Separation and Purification Technology，2009年，Vol 65 No 1，14-21
Johnson-Buck A et al，Kinetic fingerprinting to identify and count single nucleic acids，Nature Biotechnology，2015年，Vol 33 No 7，730-732

Also Published As

Publication number Publication date
EP3724317A4 (en) 2021-09-15
JP2021506253A (ja) 2021-02-22
CN111971380A (zh) 2020-11-20
US20190187031A1 (en) 2019-06-20
WO2019118705A1 (en) 2019-06-20
EP3724317A1 (en) 2020-10-21

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