CN111971380A - 分析物的浓缩 - Google Patents

分析物的浓缩 Download PDF

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Publication number
CN111971380A
CN111971380A CN201880089480.XA CN201880089480A CN111971380A CN 111971380 A CN111971380 A CN 111971380A CN 201880089480 A CN201880089480 A CN 201880089480A CN 111971380 A CN111971380 A CN 111971380A
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CN
China
Prior art keywords
analyte
atps
nucleic acid
phase
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880089480.XA
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English (en)
Chinese (zh)
Inventor
A·E·约翰逊-巴克
M·特瓦里
N·G·沃尔特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Michigan Ann Arbor
Original Assignee
University of Michigan Ann Arbor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Michigan Ann Arbor filed Critical University of Michigan Ann Arbor
Publication of CN111971380A publication Critical patent/CN111971380A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201880089480.XA 2017-12-14 2018-12-13 分析物的浓缩 Pending CN111971380A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762598802P 2017-12-14 2017-12-14
US62/598802 2017-12-14
US201862692001P 2018-06-29 2018-06-29
US62/692001 2018-06-29
PCT/US2018/065421 WO2019118705A1 (en) 2017-12-14 2018-12-13 Concentration of analytes

Publications (1)

Publication Number Publication Date
CN111971380A true CN111971380A (zh) 2020-11-20

Family

ID=66815084

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880089480.XA Pending CN111971380A (zh) 2017-12-14 2018-12-13 分析物的浓缩

Country Status (5)

Country Link
US (1) US20190187031A1 (https=)
EP (1) EP3724317A4 (https=)
JP (1) JP7270992B2 (https=)
CN (1) CN111971380A (https=)
WO (1) WO2019118705A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113324619A (zh) * 2021-06-02 2021-08-31 成都瀚辰光翼科技有限责任公司 一种基于电容检测的液面探测方法及装置

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12065698B2 (en) * 2009-10-02 2024-08-20 Thermo Fisher Scientific Baltics Uab Sample processing apparatus and method
JP2019505884A (ja) * 2015-12-10 2019-02-28 キアゲン ゲーエムベーハー デジタル画像内の少なくとも１つのオブジェクトの全体的明るさを判定するための方法
AU2018231211A1 (en) 2017-03-08 2019-10-03 The Regents Of The University Of Michigan Analyte detection
WO2018183465A1 (en) 2017-03-28 2018-10-04 Chiu Yin To Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
EP4303311A3 (en) 2017-09-18 2024-04-03 Phase Scientific International, Ltd. Method for using aqueous two-phase system for the isolation, purification and/or concentration of short nucleic acid fragments
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
WO2020023503A1 (en) * 2018-07-24 2020-01-30 The Regents Of The University Of Michigan Intramolecular kinetic probes
KR20250047414A (ko) * 2019-07-16 2025-04-03 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
WO2021154935A1 (en) 2020-01-29 2021-08-05 The Regents Of The University Of Michigan Analyte detection
WO2021188308A1 (en) * 2020-03-19 2021-09-23 The Regents Of The University Of Michigan Analyte detection
EP4121530A1 (en) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction
US20240140981A1 (en) * 2020-12-29 2024-05-02 Curative Inc. Nucleic acid purification methods using a water-immiscible solvent wash
JP2024513919A (ja) * 2021-04-06 2024-03-27 エックスゲノムズ コーポレーション 核酸のエピジェネティックな修飾を検出するためのシステム、方法、及び組成物
CN118414544A (zh) 2021-12-21 2024-07-30 生物辐射实验室股份有限公司 通过测量局部荧光比率在高分辨率装置中进行多重检测
WO2024064787A2 (en) * 2022-09-21 2024-03-28 Ultragenyx Pharmaceutical Inc. Method for determination of double-stranded rna impurity
CN119137270A (zh) 2022-11-02 2024-12-13 相达生物科技国际有限公司 使用atps和固相介质从生物样品分离目标分析物的方法
WO2024093522A1 (en) * 2022-11-02 2024-05-10 Phase Scientific International, Ltd. Methods, compositions and kits for concentrating target analytes from a bulk fluid sample
AU2023374887A1 (en) * 2022-11-02 2025-05-22 Phase Scientific International, Ltd. Methods and kits for isolating target nucleic acids below a target size from a sample
CN116103375B (zh) * 2022-11-11 2025-11-14 北京理工大学 基于dna折纸的纳米标尺及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060094033A1 (en) * 2004-05-21 2006-05-04 Carl Abulencia Screening methods and libraries of trace amounts of DNA from uncultivated microorganisms
US20110257378A1 (en) * 2008-11-25 2011-10-20 Ge Healthcare Bio-Sciences Ab Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
WO2017041030A1 (en) * 2015-09-04 2017-03-09 The Regents Of The University Of California Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
WO2017139354A1 (en) * 2016-02-10 2017-08-17 The Regents Of The University Of Michigan Detection of nucleic acids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020081629A1 (en) * 2000-06-30 2002-06-27 Tibotec N.V. Apparatus for the simultaneous transfer of liquid analytes
EP2548022B1 (en) * 2010-03-18 2016-03-16 The Regents Of The University Of Michigan Multiphase microarrays and uses thereof
EP3114482B1 (en) * 2014-03-07 2020-10-07 The Regents of the University of California Devices for integrating analyte extraction, concentration and detection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060094033A1 (en) * 2004-05-21 2006-05-04 Carl Abulencia Screening methods and libraries of trace amounts of DNA from uncultivated microorganisms
US20110257378A1 (en) * 2008-11-25 2011-10-20 Ge Healthcare Bio-Sciences Ab Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
WO2017041030A1 (en) * 2015-09-04 2017-03-09 The Regents Of The University Of California Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
WO2017139354A1 (en) * 2016-02-10 2017-08-17 The Regents Of The University Of Michigan Detection of nucleic acids

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113324619A (zh) * 2021-06-02 2021-08-31 成都瀚辰光翼科技有限责任公司 一种基于电容检测的液面探测方法及装置
CN113324619B (zh) * 2021-06-02 2023-06-06 成都瀚辰光翼科技有限责任公司 一种基于电容检测的液面探测方法及装置

Also Published As

Publication number Publication date
EP3724317A4 (en) 2021-09-15
JP2021506253A (ja) 2021-02-22
JP7270992B2 (ja) 2023-05-11
US20190187031A1 (en) 2019-06-20
WO2019118705A1 (en) 2019-06-20
EP3724317A1 (en) 2020-10-21

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