JP7267458B2 - 非天然アミノ酸を含むペプチドの製造方法 - Google Patents

非天然アミノ酸を含むペプチドの製造方法 Download PDF

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JP7267458B2
JP7267458B2 JP2021564050A JP2021564050A JP7267458B2 JP 7267458 B2 JP7267458 B2 JP 7267458B2 JP 2021564050 A JP2021564050 A JP 2021564050A JP 2021564050 A JP2021564050 A JP 2021564050A JP 7267458 B2 JP7267458 B2 JP 7267458B2
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amino acid
peptide
amino
protein
translation
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JPWO2021117848A1 (https=
JPWO2021117848A5 (https=
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早貴子 吉井
康一郎 下村
聡 石野
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Chugai Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
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  • Bioinformatics & Computational Biology (AREA)
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  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2021564050A 2019-12-12 2020-12-11 非天然アミノ酸を含むペプチドの製造方法 Active JP7267458B2 (ja)

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JP2023068770A JP7702985B2 (ja) 2019-12-12 2023-04-19 非天然アミノ酸を含むペプチドの製造方法
JP2025105913A JP2025126268A (ja) 2019-12-12 2025-06-23 非天然アミノ酸を含むペプチドの製造方法

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JP2019224297 2019-12-12
JP2019224297 2019-12-12
PCT/JP2020/046213 WO2021117848A1 (ja) 2019-12-12 2020-12-11 非天然アミノ酸を含むペプチドの製造方法

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JPWO2021117848A1 JPWO2021117848A1 (https=) 2021-06-17
JPWO2021117848A5 JPWO2021117848A5 (https=) 2022-03-08
JP7267458B2 true JP7267458B2 (ja) 2023-05-01

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JP2023068770A Active JP7702985B2 (ja) 2019-12-12 2023-04-19 非天然アミノ酸を含むペプチドの製造方法
JP2025105913A Pending JP2025126268A (ja) 2019-12-12 2025-06-23 非天然アミノ酸を含むペプチドの製造方法

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JP2025105913A Pending JP2025126268A (ja) 2019-12-12 2025-06-23 非天然アミノ酸を含むペプチドの製造方法

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US (1) US20230069218A1 (https=)
EP (1) EP4074829A4 (https=)
JP (3) JP7267458B2 (https=)
KR (1) KR20220113729A (https=)
CN (1) CN114787359A (https=)
WO (1) WO2021117848A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
JPWO2024128195A1 (https=) 2022-12-12 2024-06-20
WO2024214303A1 (ja) 2023-04-14 2024-10-17 中外製薬株式会社 複数種類の標的分子との複合体を形成し得る目的分子のスクリーニング方法、及び当該スクリーニング方法を含む目的分子の製造方法

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
JPH078289A (ja) * 1993-06-29 1995-01-13 Osaka Gas Co Ltd リボソームを用いたペプチドおよびタンパク質の合成法
JP4441170B2 (ja) * 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
CN101048506B (zh) * 2004-10-27 2013-06-26 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
WO2008073184A2 (en) * 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
JP5605602B2 (ja) 2007-03-26 2014-10-15 国立大学法人 東京大学 環状ペプチド化合物の合成方法
JP5818237B2 (ja) * 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647721B1 (en) 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7073250B2 (ja) 2016-03-03 2022-05-23 中外製薬株式会社 チオール基をアミノ基近傍に有するアミノ酸をｎ末端に持つ非環状ペプチド－核酸複合体、そのライブラリー、およびそれから誘導される環状ペプチド－核酸複合体ライブラリーの製造方法
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
RU2731211C1 (ru) * 2017-03-21 2020-08-31 Фуджифилм Корпорэйшн Пептидное соединение и способ его получения, композиция для скринингового использования и способ отбора пептидного соединения
CN110869544B (zh) 2017-06-09 2024-03-08 中外制药株式会社 膜透过性高的环状肽化合物及包含其的文库
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3904568A4 (en) 2018-12-26 2024-03-20 Chugai Seiyaku Kabushiki Kaisha Mutated trna for codon expansion

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
UETA, M. et al.，Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translation, whereas short L31 cleaved by protease 7 reduces both activities，Genes to Cells，2017年，vol.22，p.452-471
UniprotKB-A0A377B8T0，2018年11月07日，UniProt [online], [retrieved on 2021.01.14], Retrieved from the Internet : <URL: https://www.uniprot.org/uniprot/A0A377B8T0>, Accession No. A0A377B8T0
上田雅美等，大腸菌リボソームの翻訳活性は従来の測定値より４０％高い ～Ｌ３１蛋白損傷の影響を排除する～，第３９回日本分子生物学会年会要旨集，2016年，vol.39，Abstract No. 3P-0236

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Publication number Publication date
JP2025126268A (ja) 2025-08-28
WO2021117848A1 (ja) 2021-06-17
KR20220113729A (ko) 2022-08-16
JP2023099039A (ja) 2023-07-11
JPWO2021117848A1 (https=) 2021-06-17
JP7702985B2 (ja) 2025-07-04
US20230069218A1 (en) 2023-03-02
EP4074829A4 (en) 2024-01-10
CN114787359A (zh) 2022-07-22
EP4074829A1 (en) 2022-10-19

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