JPWO2021117848A1 - - Google Patents
Info
- Publication number
- JPWO2021117848A1 JPWO2021117848A1 JP2021564050A JP2021564050A JPWO2021117848A1 JP WO2021117848 A1 JPWO2021117848 A1 JP WO2021117848A1 JP 2021564050 A JP2021564050 A JP 2021564050A JP 2021564050 A JP2021564050 A JP 2021564050A JP WO2021117848 A1 JPWO2021117848 A1 JP WO2021117848A1
- Authority
- JP
- Japan
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023068770A JP7702985B2 (ja) | 2019-12-12 | 2023-04-19 | 非天然アミノ酸を含むペプチドの製造方法 |
| JP2025105913A JP2025126268A (ja) | 2019-12-12 | 2025-06-23 | 非天然アミノ酸を含むペプチドの製造方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019224297 | 2019-12-12 | ||
| JP2019224297 | 2019-12-12 | ||
| PCT/JP2020/046213 WO2021117848A1 (ja) | 2019-12-12 | 2020-12-11 | 非天然アミノ酸を含むペプチドの製造方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023068770A Division JP7702985B2 (ja) | 2019-12-12 | 2023-04-19 | 非天然アミノ酸を含むペプチドの製造方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2021117848A1 true JPWO2021117848A1 (https=) | 2021-06-17 |
| JPWO2021117848A5 JPWO2021117848A5 (https=) | 2022-03-08 |
| JP7267458B2 JP7267458B2 (ja) | 2023-05-01 |
Family
ID=76329976
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021564050A Active JP7267458B2 (ja) | 2019-12-12 | 2020-12-11 | 非天然アミノ酸を含むペプチドの製造方法 |
| JP2023068770A Active JP7702985B2 (ja) | 2019-12-12 | 2023-04-19 | 非天然アミノ酸を含むペプチドの製造方法 |
| JP2025105913A Pending JP2025126268A (ja) | 2019-12-12 | 2025-06-23 | 非天然アミノ酸を含むペプチドの製造方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023068770A Active JP7702985B2 (ja) | 2019-12-12 | 2023-04-19 | 非天然アミノ酸を含むペプチドの製造方法 |
| JP2025105913A Pending JP2025126268A (ja) | 2019-12-12 | 2025-06-23 | 非天然アミノ酸を含むペプチドの製造方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230069218A1 (https=) |
| EP (1) | EP4074829A4 (https=) |
| JP (3) | JP7267458B2 (https=) |
| KR (1) | KR20220113729A (https=) |
| CN (1) | CN114787359A (https=) |
| WO (1) | WO2021117848A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW202446781A (zh) | 2011-12-28 | 2024-12-01 | 日商中外製藥股份有限公司 | 胜肽化合物之製造方法 |
| EP4166664A1 (en) | 2015-03-13 | 2023-04-19 | Chugai Seiyaku Kabushiki Kaisha | Modified aminoacyl-trna synthetase and use thereof |
| JP7187323B2 (ja) | 2017-01-31 | 2022-12-12 | 中外製薬株式会社 | 無細胞翻訳系におけるペプチドの合成方法 |
| JPWO2024128195A1 (https=) | 2022-12-12 | 2024-06-20 | ||
| WO2024214303A1 (ja) | 2023-04-14 | 2024-10-17 | 中外製薬株式会社 | 複数種類の標的分子との複合体を形成し得る目的分子のスクリーニング方法、及び当該スクリーニング方法を含む目的分子の製造方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH078289A (ja) * | 1993-06-29 | 1995-01-13 | Osaka Gas Co Ltd | リボソームを用いたペプチドおよびタンパク質の合成法 |
| JP4441170B2 (ja) * | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
| CN101048506B (zh) * | 2004-10-27 | 2013-06-26 | 斯克利普斯研究院 | 体内掺入非天然氨基酸的正交翻译组分 |
| WO2008073184A2 (en) * | 2006-10-18 | 2008-06-19 | The Scripps Research Institute | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
| JP5605602B2 (ja) | 2007-03-26 | 2014-10-15 | 国立大学法人 東京大学 | 環状ペプチド化合物の合成方法 |
| JP5818237B2 (ja) * | 2010-09-09 | 2015-11-18 | 国立大学法人 東京大学 | Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法 |
| EP2647721B1 (en) | 2010-12-03 | 2019-02-27 | The University of Tokyo | Peptide with safer secondary structure, peptide library, and production methods for same |
| TW202446781A (zh) | 2011-12-28 | 2024-12-01 | 日商中外製藥股份有限公司 | 胜肽化合物之製造方法 |
| EP4166664A1 (en) | 2015-03-13 | 2023-04-19 | Chugai Seiyaku Kabushiki Kaisha | Modified aminoacyl-trna synthetase and use thereof |
| JP7073250B2 (ja) | 2016-03-03 | 2022-05-23 | 中外製薬株式会社 | チオール基をアミノ基近傍に有するアミノ酸をｎ末端に持つ非環状ペプチド－核酸複合体、そのライブラリー、およびそれから誘導される環状ペプチド－核酸複合体ライブラリーの製造方法 |
| JP7187323B2 (ja) | 2017-01-31 | 2022-12-12 | 中外製薬株式会社 | 無細胞翻訳系におけるペプチドの合成方法 |
| RU2731211C1 (ru) * | 2017-03-21 | 2020-08-31 | Фуджифилм Корпорэйшн | Пептидное соединение и способ его получения, композиция для скринингового использования и способ отбора пептидного соединения |
| CN110869544B (zh) | 2017-06-09 | 2024-03-08 | 中外制药株式会社 | 膜透过性高的环状肽化合物及包含其的文库 |
| KR20250007021A (ko) | 2017-06-09 | 2025-01-13 | 추가이 세이야쿠 가부시키가이샤 | N-치환 아미노산을 포함하는 펩타이드의 합성 방법 |
| EP3904568A4 (en) | 2018-12-26 | 2024-03-20 | Chugai Seiyaku Kabushiki Kaisha | Mutated trna for codon expansion |
-
2020
- 2020-12-11 JP JP2021564050A patent/JP7267458B2/ja active Active
- 2020-12-11 US US17/783,076 patent/US20230069218A1/en active Pending
- 2020-12-11 WO PCT/JP2020/046213 patent/WO2021117848A1/ja not_active Ceased
- 2020-12-11 EP EP20899836.9A patent/EP4074829A4/en active Pending
- 2020-12-11 CN CN202080085341.7A patent/CN114787359A/zh active Pending
- 2020-12-11 KR KR1020227022165A patent/KR20220113729A/ko active Pending
-
2023
- 2023-04-19 JP JP2023068770A patent/JP7702985B2/ja active Active
-
2025
- 2025-06-23 JP JP2025105913A patent/JP2025126268A/ja active Pending
Non-Patent Citations (3)
| Title |
|---|
| UETA, M. ET AL.: "Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translatio", GENES TO CELLS, vol. 22, JPN6021002266, 2017, pages 452 - 471, XP055835088, ISSN: 0004916292, DOI: 10.1111/gtc.12488 * |
| UNIPROTKB-A0A377B8T0, JPN6021002269, 7 November 2018 (2018-11-07), ISSN: 0004916294 * |
| 上田雅美等: "大腸菌リボソームの翻訳活性は従来の測定値より４０％高い 〜Ｌ３１蛋白損傷の影響を排除する〜", 第３９回日本分子生物学会年会要旨集, vol. 39, JPN6021002268, 2016, pages 3 - 0236, ISSN: 0004916293 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2025126268A (ja) | 2025-08-28 |
| WO2021117848A1 (ja) | 2021-06-17 |
| KR20220113729A (ko) | 2022-08-16 |
| JP2023099039A (ja) | 2023-07-11 |
| JP7702985B2 (ja) | 2025-07-04 |
| US20230069218A1 (en) | 2023-03-02 |
| EP4074829A4 (en) | 2024-01-10 |
| CN114787359A (zh) | 2022-07-22 |
| JP7267458B2 (ja) | 2023-05-01 |
| EP4074829A1 (en) | 2022-10-19 |
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