CN114787359A - 产生含有非天然氨基酸的肽的方法 - Google Patents

产生含有非天然氨基酸的肽的方法 Download PDF

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Publication number
CN114787359A
CN114787359A CN202080085341.7A CN202080085341A CN114787359A CN 114787359 A CN114787359 A CN 114787359A CN 202080085341 A CN202080085341 A CN 202080085341A CN 114787359 A CN114787359 A CN 114787359A
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amino acid
protein
group
peptide
ribosomes
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Pending
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Chinese (zh)
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吉井早贵子
下村康一郎
石野聪
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Chugai Pharmaceutical Co Ltd
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Chugai Pharmaceutical Co Ltd
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
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  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN202080085341.7A 2019-12-12 2020-12-11 产生含有非天然氨基酸的肽的方法 Pending CN114787359A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-224297 2019-12-12
JP2019224297 2019-12-12
PCT/JP2020/046213 WO2021117848A1 (ja) 2019-12-12 2020-12-11 非天然アミノ酸を含むペプチドの製造方法

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Publication Number Publication Date
CN114787359A true CN114787359A (zh) 2022-07-22

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CN202080085341.7A Pending CN114787359A (zh) 2019-12-12 2020-12-11 产生含有非天然氨基酸的肽的方法

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US (1) US20230069218A1 (https=)
EP (1) EP4074829A4 (https=)
JP (3) JP7267458B2 (https=)
KR (1) KR20220113729A (https=)
CN (1) CN114787359A (https=)
WO (1) WO2021117848A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
JPWO2024128195A1 (https=) 2022-12-12 2024-06-20
WO2024214303A1 (ja) 2023-04-14 2024-10-17 中外製薬株式会社 複数種類の標的分子との複合体を形成し得る目的分子のスクリーニング方法、及び当該スクリーニング方法を含む目的分子の製造方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004173627A (ja) * 2002-11-28 2004-06-24 Inst Of Physical & Chemical Res Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
CN101535338A (zh) * 2006-10-18 2009-09-16 斯克利普斯研究院 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质
CN102827827A (zh) * 2004-10-27 2012-12-19 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
WO2018174078A1 (ja) * 2017-03-21 2018-09-27 富士フイルム株式会社 ペプチド化合物およびその製造方法、スクリーニング用組成物、並びにペプチド化合物の選択方法

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JPH078289A (ja) * 1993-06-29 1995-01-13 Osaka Gas Co Ltd リボソームを用いたペプチドおよびタンパク質の合成法
JP5605602B2 (ja) 2007-03-26 2014-10-15 国立大学法人 東京大学 環状ペプチド化合物の合成方法
JP5818237B2 (ja) * 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647721B1 (en) 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7073250B2 (ja) 2016-03-03 2022-05-23 中外製薬株式会社 チオール基をアミノ基近傍に有するアミノ酸をｎ末端に持つ非環状ペプチド－核酸複合体、そのライブラリー、およびそれから誘導される環状ペプチド－核酸複合体ライブラリーの製造方法
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
CN110869544B (zh) 2017-06-09 2024-03-08 中外制药株式会社 膜透过性高的环状肽化合物及包含其的文库
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3904568A4 (en) 2018-12-26 2024-03-20 Chugai Seiyaku Kabushiki Kaisha Mutated trna for codon expansion

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JP2004173627A (ja) * 2002-11-28 2004-06-24 Inst Of Physical & Chemical Res Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
CN102827827A (zh) * 2004-10-27 2012-12-19 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
CN101535338A (zh) * 2006-10-18 2009-09-16 斯克利普斯研究院 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质
WO2018174078A1 (ja) * 2017-03-21 2018-09-27 富士フイルム株式会社 ペプチド化合物およびその製造方法、スクリーニング用組成物、並びにペプチド化合物の選択方法

Non-Patent Citations (4)

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MASAMI UETA等: ""Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translation, whereas short L31 cleaved by protease 7 reduces both activities"", 《GENES CELLS》, vol. 22, no. 5, pages 454 *
井健: ""使用无细胞翻译体系合成尿激酶原突变体蛋白"", 《北京师范大学学报(自然科学版)》, vol. 44, no. 02, 15 April 2008 (2008-04-15), pages 185 *
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Publication number Publication date
JP2025126268A (ja) 2025-08-28
WO2021117848A1 (ja) 2021-06-17
KR20220113729A (ko) 2022-08-16
JP2023099039A (ja) 2023-07-11
JPWO2021117848A1 (https=) 2021-06-17
JP7702985B2 (ja) 2025-07-04
US20230069218A1 (en) 2023-03-02
EP4074829A4 (en) 2024-01-10
JP7267458B2 (ja) 2023-05-01
EP4074829A1 (en) 2022-10-19

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