JP7248590B2 - 二分化または多分化オルガノイド - Google Patents

二分化または多分化オルガノイド Download PDF

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JP7248590B2
JP7248590B2 JP2019557794A JP2019557794A JP7248590B2 JP 7248590 B2 JP7248590 B2 JP 7248590B2 JP 2019557794 A JP2019557794 A JP 2019557794A JP 2019557794 A JP2019557794 A JP 2019557794A JP 7248590 B2 JP7248590 B2 JP 7248590B2
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クノーブリヒ ユルゲン
エー．バグリー ジョシュア
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イーエムベーアー－インスティテュート フュール モレクラレ バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング
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JP2019557794A 2017-04-25 2018-04-25 二分化または多分化オルガノイド Active JP7248590B2 (ja)

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EP17168051.5A EP3395942A1 (en) 2017-04-25 2017-04-25 Bi- or multi-differentiated organoid
EP17168051.5 2017-04-25
PCT/EP2018/060559 WO2018197544A1 (en) 2017-04-25 2018-04-25 Bi- or multi-differentiated organoid

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JP2020517283A JP2020517283A (ja) 2020-06-18
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EP (2) EP3395942A1 (https=)
JP (1) JP7248590B2 (https=)
KR (1) KR102692498B1 (https=)
CN (2) CN110573609A (https=)
AU (1) AU2018260183B2 (https=)
CA (1) CA3061412A1 (https=)
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Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
CN106661548B (zh) 2014-05-28 2020-12-11 儿童医院医疗中心 用于经由定向分化将前体细胞转化为胃组织的方法和系统
JP6804438B2 (ja) 2014-10-17 2020-12-23 チルドレンズ ホスピタル メディカル センター 多能性幹細胞を使用するヒト小腸のｉｎ ｖｉｖｏモデル、並びにそれを作製、及び使用する方法
ES2929758T3 (es) 2016-05-05 2022-12-01 Childrens Hospital Med Ct Métodos para la fabricación in vitro de tejido del fondo gástrico y composiciones relacionadas con el mismo
KR102729404B1 (ko) 2016-11-04 2024-11-14 칠드런즈 호스피탈 메디칼 센터 간 유사 장기 조성물 및 이를 제조 및 사용하는 방법
JP7068305B2 (ja) 2016-12-05 2022-05-16 チルドレンズ ホスピタル メディカル センター 結腸オルガノイドならびにその作製方法および使用方法
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
CA3071454A1 (en) * 2017-07-28 2019-01-31 Memorial Sloan-Kettering Cancer Center Establishing topographic organization in three-dimensional tissue culture
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3720452A4 (en) * 2017-12-04 2022-02-23 The Administrators of The Tulane Educational Fund CELL SYSTEMS USING SPHEROIDS AND METHODS FOR THEIR MANUFACTURE AND USE
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
KR102887406B1 (ko) 2018-07-26 2025-11-19 칠드런즈 호스피탈 메디칼 센터 간-담도-췌장 조직 및 이를 제조하는 방법
AU2019339410A1 (en) 2018-09-12 2021-04-15 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
WO2020243633A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
WO2020243613A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
WO2022010901A2 (en) * 2020-07-06 2022-01-13 President And Fellows Of Harvard College Programmable organoids and methods of producing the same via orthogonal differentiation and bioprinting
WO2022154080A1 (ja) * 2021-01-15 2022-07-21 国立大学法人東京大学 複雑な活動と可塑性を示すインビトロ神経回路組織、その神経活動の観察装置およびこれを用いた物質のスクリーニング方法
DE102021132190A1 (de) * 2021-12-07 2023-06-22 Eberhard Karls Universität Tübingen, Körperschaft des öffentlichen Rechts Biokompatible Strukturen zur Verbindung und Kultivierung von biologischem Material
HUP2200189A2 (hu) * 2022-05-30 2023-12-28 Biotalentum Tudasfejlesztoe Kft Új, riportert stabilan expresszáló humán indukált pluripotens õssejt
EP4286513A1 (en) 2022-06-03 2023-12-06 IMBA-Institut für Molekulare Biotechnologie GmbH Triple tissue culture fusion
CN116286342B (zh) * 2022-12-23 2025-10-28 山东大学 一种类人脑皮质芯片、3d打印类人脑皮质的方法及应用
CN116129193B (zh) * 2023-02-27 2024-06-11 重庆大学附属肿瘤医院 一种类器官生长预测方法、系统及设备
CN118530833B (zh) * 2024-04-02 2024-11-29 武汉大学 一种同步共分化的多组织或多类器官模型及其构建方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013509859A (ja) 2009-11-05 2013-03-21 独立行政法人理化学研究所 幹細胞の分化誘導方法
WO2015076388A1 (ja) 2013-11-22 2015-05-28 国立研究開発法人理化学研究所 終脳又はその前駆組織の製造方法
JP2015528289A (ja) 2012-09-03 2015-09-28 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
JP2016506244A (ja) 2012-12-13 2016-03-03 イーエムベーアー−インスティテュート フュール モレクラレ バイオテクロノジー ゲゼルシャフト ミットベシュレンクテル ハフツング 三次元不均一分化組織培養
WO2016141137A1 (en) 2015-03-03 2016-09-09 President And Fellows Of Harvard College Methods of generating functional human tissue
WO2017060884A1 (en) 2015-10-08 2017-04-13 Université Du Luxembourg Means and methods for generating midbrain organoids

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
DE10003521A1 (de) 2000-01-27 2001-08-09 Medigene Ag Vorrichtung zum Herstellen eines dreidimensionalen Matrixkörpers, Multi-Well-Platte, Lösung zum Kultivieren von Säugerkardiomyocyten, Verfahren zum Kultivieren einer Zellkultur, Vorrichtung für die Messung isometrischer Kraftparameter von Zellkulturen sowie Verfahren zum meßbaren Verfolgen von Kontraktionen eines in eine Trägersubstanz eingelagerten Zellgewebes
JP4330995B2 (ja) 2001-11-15 2009-09-16 チルドレンズ メディカル センター コーポレーション 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法
WO2004084950A2 (en) 2003-03-24 2004-10-07 Case Western Reserve University Cell targeting methods and compositions
US20100196923A1 (en) 2007-08-14 2010-08-05 Anthony Atala Pluripotent adult stem cells
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
US20110143433A1 (en) 2008-03-17 2011-06-16 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
WO2012013936A1 (en) * 2010-07-30 2012-02-02 Cambridge Enterprise Limited Corticogenesis of human pluripotent cells
KR101381798B1 (ko) * 2013-02-27 2014-04-07 씨제이제일제당 (주) 신규 박테리오파지 및 이를 포함하는 항균 조성물
WO2014153346A1 (en) * 2013-03-18 2014-09-25 Massachusetts Institute Of Technology Engineering a heterogeneous tissue pluripotent stem cells
EP3498824A1 (en) * 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
US10794802B2 (en) * 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
WO2015069736A1 (en) * 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
US10081792B2 (en) * 2014-12-31 2018-09-25 Wisconsin Alumni Research Foundation Derivation of human microglia from pluripotent stem cells
US10087417B2 (en) * 2015-04-22 2018-10-02 William J. Freed Three-dimensional model of human cortex
US10081791B2 (en) * 2015-05-07 2018-09-25 William J. Freed Modeling connections between dopaminergic neurons and the cerebral cortex
WO2017083705A1 (en) * 2015-11-13 2017-05-18 The Johns Hopkins University Cell culture system and method of use thereof
WO2017117547A1 (en) * 2015-12-31 2017-07-06 President And Fellows Of Harvard College Methods for generating neural tissue and uses thereof
SG11201807487WA (en) * 2016-03-14 2018-09-27 Agency Science Tech & Res Generation of midbrain-specific organoids from human pluripotent stem cells
FR3058892B1 (fr) * 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere
EP3600362B1 (en) * 2017-03-28 2023-12-13 The Board of Trustees of the Leland Stanford Junior University Assembly of functionally integrated human forebrain spheroids and methods of use thereof
KR20240124440A (ko) * 2017-03-29 2024-08-16 이스트만 케미칼 컴파니 위치-선택적으로 치환된 셀룰로스 에스터
GB201900930D0 (en) * 2019-01-23 2019-03-13 Res & Innovation Uk Choroid plexus organoids and methods for productions thereof
WO2020247336A1 (en) * 2019-06-03 2020-12-10 The Regents Of The University Of California Pifithrin analogues and methods of treating rett syndrome

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013509859A (ja) 2009-11-05 2013-03-21 独立行政法人理化学研究所 幹細胞の分化誘導方法
JP2015528289A (ja) 2012-09-03 2015-09-28 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
JP2016506244A (ja) 2012-12-13 2016-03-03 イーエムベーアー−インスティテュート フュール モレクラレ バイオテクロノジー ゲゼルシャフト ミットベシュレンクテル ハフツング 三次元不均一分化組織培養
WO2015076388A1 (ja) 2013-11-22 2015-05-28 国立研究開発法人理化学研究所 終脳又はその前駆組織の製造方法
WO2016141137A1 (en) 2015-03-03 2016-09-09 President And Fellows Of Harvard College Methods of generating functional human tissue
WO2017060884A1 (en) 2015-10-08 2017-04-13 Université Du Luxembourg Means and methods for generating midbrain organoids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LANCASTER M.A. et al., 'Cerebral organoids model human brain development and microcephaly', NATURE (2013), Vol.501, No.7467, pp.373-379, DOI: 10.1038/nature12517

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CN110573609A (zh) 2019-12-13
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