CN110573609A - 双分化或多分化类器官 - Google Patents

双分化或多分化类器官 Download PDF

Info

Publication number
CN110573609A
CN110573609A CN201880027801.3A CN201880027801A CN110573609A CN 110573609 A CN110573609 A CN 110573609A CN 201880027801 A CN201880027801 A CN 201880027801A CN 110573609 A CN110573609 A CN 110573609A
Authority
CN
China
Prior art keywords
tissue
cells
ventral
differentiation
tissues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880027801.3A
Other languages
English (en)
Chinese (zh)
Inventor
J.诺布里奇
J.A.巴格利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imba Molikul Institute Of Biotechnology
Original Assignee
Imba Molikul Institute Of Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imba Molikul Institute Of Biotechnology filed Critical Imba Molikul Institute Of Biotechnology
Priority to CN202411042018.8A priority Critical patent/CN119101651A/zh
Publication of CN110573609A publication Critical patent/CN110573609A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0697Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0619Neurons
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082Supracellular entities, e.g. tissue, organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/41Hedgehog proteins; Cyclopamine (inhibitor)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/08Coculture with; Conditioned medium produced by cells of the nervous system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880027801.3A 2017-04-25 2018-04-25 双分化或多分化类器官 Pending CN110573609A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202411042018.8A CN119101651A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17168051.5A EP3395942A1 (en) 2017-04-25 2017-04-25 Bi- or multi-differentiated organoid
EP17168051.5 2017-04-25
PCT/EP2018/060559 WO2018197544A1 (en) 2017-04-25 2018-04-25 Bi- or multi-differentiated organoid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202411042018.8A Division CN119101651A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Publications (1)

Publication Number Publication Date
CN110573609A true CN110573609A (zh) 2019-12-13

Family

ID=58672346

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201880027801.3A Pending CN110573609A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官
CN202411042018.8A Pending CN119101651A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202411042018.8A Pending CN119101651A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Country Status (9)

Country Link
US (1) US20210095260A1 (https=)
EP (2) EP3395942A1 (https=)
JP (1) JP7248590B2 (https=)
KR (1) KR102692498B1 (https=)
CN (2) CN110573609A (https=)
AU (1) AU2018260183B2 (https=)
CA (1) CA3061412A1 (https=)
SG (1) SG11201909470TA (https=)
WO (1) WO2018197544A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116129193A (zh) * 2023-02-27 2023-05-16 重庆大学附属肿瘤医院 一种类器官生长预测方法、系统及设备
CN118530833A (zh) * 2024-04-02 2024-08-23 武汉大学 一种同步共分化的多组织或多类器官模型及其构建方法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
CN106661548B (zh) 2014-05-28 2020-12-11 儿童医院医疗中心 用于经由定向分化将前体细胞转化为胃组织的方法和系统
JP6804438B2 (ja) 2014-10-17 2020-12-23 チルドレンズ ホスピタル メディカル センター 多能性幹細胞を使用するヒト小腸のin vivoモデル、並びにそれを作製、及び使用する方法
ES2929758T3 (es) 2016-05-05 2022-12-01 Childrens Hospital Med Ct Métodos para la fabricación in vitro de tejido del fondo gástrico y composiciones relacionadas con el mismo
KR102729404B1 (ko) 2016-11-04 2024-11-14 칠드런즈 호스피탈 메디칼 센터 간 유사 장기 조성물 및 이를 제조 및 사용하는 방법
JP7068305B2 (ja) 2016-12-05 2022-05-16 チルドレンズ ホスピタル メディカル センター 結腸オルガノイドならびにその作製方法および使用方法
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
CA3071454A1 (en) * 2017-07-28 2019-01-31 Memorial Sloan-Kettering Cancer Center Establishing topographic organization in three-dimensional tissue culture
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3720452A4 (en) * 2017-12-04 2022-02-23 The Administrators of The Tulane Educational Fund CELL SYSTEMS USING SPHEROIDS AND METHODS FOR THEIR MANUFACTURE AND USE
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
KR102887406B1 (ko) 2018-07-26 2025-11-19 칠드런즈 호스피탈 메디칼 센터 간-담도-췌장 조직 및 이를 제조하는 방법
AU2019339410A1 (en) 2018-09-12 2021-04-15 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
WO2020243633A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
WO2020243613A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
WO2022010901A2 (en) * 2020-07-06 2022-01-13 President And Fellows Of Harvard College Programmable organoids and methods of producing the same via orthogonal differentiation and bioprinting
WO2022154080A1 (ja) * 2021-01-15 2022-07-21 国立大学法人東京大学 複雑な活動と可塑性を示すインビトロ神経回路組織、その神経活動の観察装置およびこれを用いた物質のスクリーニング方法
DE102021132190A1 (de) * 2021-12-07 2023-06-22 Eberhard Karls Universität Tübingen, Körperschaft des öffentlichen Rechts Biokompatible Strukturen zur Verbindung und Kultivierung von biologischem Material
HUP2200189A2 (hu) * 2022-05-30 2023-12-28 Biotalentum Tudasfejlesztoe Kft Új, riportert stabilan expresszáló humán indukált pluripotens õssejt
EP4286513A1 (en) 2022-06-03 2023-12-06 IMBA-Institut für Molekulare Biotechnologie GmbH Triple tissue culture fusion
CN116286342B (zh) * 2022-12-23 2025-10-28 山东大学 一种类人脑皮质芯片、3d打印类人脑皮质的方法及应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009024448A1 (en) * 2007-08-20 2009-02-26 Universite Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
WO2014176606A1 (en) * 2013-04-26 2014-10-30 Memorial Sloan-Kettering Center Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
WO2015069736A1 (en) * 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
US20150330970A1 (en) * 2012-12-13 2015-11-19 Imba - Institut Fur Molekulare Biotechnologie Gmbh Three dimensional heterogeneously differentiated tissue culture
CN105209610A (zh) * 2013-02-27 2015-12-30 Cj第一制糖株式会社 新颖噬菌体和包括所述新颖噬菌体的抗细菌组成物
WO2017060884A1 (en) * 2015-10-08 2017-04-13 Université Du Luxembourg Means and methods for generating midbrain organoids

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
DE10003521A1 (de) 2000-01-27 2001-08-09 Medigene Ag Vorrichtung zum Herstellen eines dreidimensionalen Matrixkörpers, Multi-Well-Platte, Lösung zum Kultivieren von Säugerkardiomyocyten, Verfahren zum Kultivieren einer Zellkultur, Vorrichtung für die Messung isometrischer Kraftparameter von Zellkulturen sowie Verfahren zum meßbaren Verfolgen von Kontraktionen eines in eine Trägersubstanz eingelagerten Zellgewebes
JP4330995B2 (ja) 2001-11-15 2009-09-16 チルドレンズ メディカル センター コーポレーション 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法
WO2004084950A2 (en) 2003-03-24 2004-10-07 Case Western Reserve University Cell targeting methods and compositions
US20100196923A1 (en) 2007-08-14 2010-08-05 Anthony Atala Pluripotent adult stem cells
US20110143433A1 (en) 2008-03-17 2011-06-16 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
JP5787370B2 (ja) 2009-11-05 2015-09-30 国立研究開発法人理化学研究所 幹細胞の分化誘導方法
WO2012013936A1 (en) * 2010-07-30 2012-02-02 Cambridge Enterprise Limited Corticogenesis of human pluripotent cells
EP2893000B1 (en) * 2012-09-03 2019-04-10 Novo Nordisk A/S Generation of pancreatic endoderm from pluripotent stem cells using small molecules
WO2014153346A1 (en) * 2013-03-18 2014-09-25 Massachusetts Institute Of Technology Engineering a heterogeneous tissue pluripotent stem cells
US10794802B2 (en) * 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
JP6499084B2 (ja) 2013-11-22 2019-04-10 国立研究開発法人理化学研究所 終脳又はその前駆組織の製造方法
US10081792B2 (en) * 2014-12-31 2018-09-25 Wisconsin Alumni Research Foundation Derivation of human microglia from pluripotent stem cells
JP6807853B2 (ja) 2015-03-03 2021-01-06 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的ヒト組織の作製方法
US10087417B2 (en) * 2015-04-22 2018-10-02 William J. Freed Three-dimensional model of human cortex
US10081791B2 (en) * 2015-05-07 2018-09-25 William J. Freed Modeling connections between dopaminergic neurons and the cerebral cortex
WO2017083705A1 (en) * 2015-11-13 2017-05-18 The Johns Hopkins University Cell culture system and method of use thereof
WO2017117547A1 (en) * 2015-12-31 2017-07-06 President And Fellows Of Harvard College Methods for generating neural tissue and uses thereof
SG11201807487WA (en) * 2016-03-14 2018-09-27 Agency Science Tech & Res Generation of midbrain-specific organoids from human pluripotent stem cells
FR3058892B1 (fr) * 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere
EP3600362B1 (en) * 2017-03-28 2023-12-13 The Board of Trustees of the Leland Stanford Junior University Assembly of functionally integrated human forebrain spheroids and methods of use thereof
KR20240124440A (ko) * 2017-03-29 2024-08-16 이스트만 케미칼 컴파니 위치-선택적으로 치환된 셀룰로스 에스터
GB201900930D0 (en) * 2019-01-23 2019-03-13 Res & Innovation Uk Choroid plexus organoids and methods for productions thereof
WO2020247336A1 (en) * 2019-06-03 2020-12-10 The Regents Of The University Of California Pifithrin analogues and methods of treating rett syndrome

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009024448A1 (en) * 2007-08-20 2009-02-26 Universite Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
US20150330970A1 (en) * 2012-12-13 2015-11-19 Imba - Institut Fur Molekulare Biotechnologie Gmbh Three dimensional heterogeneously differentiated tissue culture
CN105209610A (zh) * 2013-02-27 2015-12-30 Cj第一制糖株式会社 新颖噬菌体和包括所述新颖噬菌体的抗细菌组成物
WO2014176606A1 (en) * 2013-04-26 2014-10-30 Memorial Sloan-Kettering Center Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
WO2015069736A1 (en) * 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
US20160272940A1 (en) * 2013-11-08 2016-09-22 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
WO2017060884A1 (en) * 2015-10-08 2017-04-13 Université Du Luxembourg Means and methods for generating midbrain organoids

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FIKRI BIREY等: "Assembly of functionally integrated human forebrain spheroids", NATURE, pages 54 - 59 *
HEIKE FRANKE等: "Dopaminergic neurons develop axonal projections to their target areas in organotypic co-cultures of the ventral mesencephalon and the striatum/prefrontal cortex", NEUROCHEMISTRY INTERNATIONAL, vol. 42, no. 5, pages 431 - 439 *
JOSHUA A BAGLEY等: "Fused dorsal-ventral cerebral organoids model complex interactions between diverse brain regions", NAT METHODS ., vol. 14, no. 7, pages 743 - 751 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116129193A (zh) * 2023-02-27 2023-05-16 重庆大学附属肿瘤医院 一种类器官生长预测方法、系统及设备
CN116129193B (zh) * 2023-02-27 2024-06-11 重庆大学附属肿瘤医院 一种类器官生长预测方法、系统及设备
CN118530833A (zh) * 2024-04-02 2024-08-23 武汉大学 一种同步共分化的多组织或多类器官模型及其构建方法
CN118530833B (zh) * 2024-04-02 2024-11-29 武汉大学 一种同步共分化的多组织或多类器官模型及其构建方法

Also Published As

Publication number Publication date
EP3615661B1 (en) 2026-02-11
JP2020517283A (ja) 2020-06-18
CA3061412A1 (en) 2018-11-01
SG11201909470TA (en) 2019-11-28
EP3615661C0 (en) 2026-02-11
EP3395942A1 (en) 2018-10-31
AU2018260183A1 (en) 2019-10-31
CN119101651A (zh) 2024-12-10
EP3615661A1 (en) 2020-03-04
AU2018260183B2 (en) 2024-09-19
WO2018197544A1 (en) 2018-11-01
JP7248590B2 (ja) 2023-03-29
KR102692498B1 (ko) 2024-08-05
US20210095260A1 (en) 2021-04-01
KR20190141231A (ko) 2019-12-23

Similar Documents

Publication Publication Date Title
KR102692498B1 (ko) 이중 또는 다중으로 분화된 오르가노이드
Bagley et al. Fused cerebral organoids model interactions between brain regions
AU2014280843B2 (en) Renal progenitor cells
KR102656200B1 (ko) 혈관 오가노이드, 상기 오가노이드의 제조 및 사용 방법
KR102555627B1 (ko) 신장 오가노이드를 형성시키기 위한 다능성 줄기세포의 분화
Ribatti et al. Epithelial-mesenchymal interactions: a fundamental developmental biology mechanism
US20210348121A1 (en) Human Polarised Three-Dimensional Cellular Aggregates
Völkner et al. Mouse retinal organoid growth and maintenance in longer-term culture
Behtaj et al. The role of PGS/PCL scaffolds in promoting differentiation of human embryonic stem cells into retinal ganglion cells
JPWO2004104184A1 (ja) 内胚葉系幹細胞の調製
Olmsted et al. Fully characterized mature human iPS-and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity
WO2023233002A1 (en) Triple tissue culture fusion
Eintracht et al. Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells
EP3872168A1 (en) Chondrocyte differentiation
Bagley et al. Fused dorsal-ventral cerebral organoids model human cortical interneuron migration
HK40014216A (en) Bi- or multi-differentiated organoid
Ferreira Integrating 3D Bioprinting and Pluripotent Stem Cell Technologies for Articular Cartilage Tissue Engineering
WO2025070803A1 (en) Axioloid-based modular bottom-up in vitro model of human post-gastrulation development
Alsehli et al. Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids
Ludwig Packaging Photoreceptors for Cell Replacement Therapy: Optimizing Form for Function
Carré Vers un modèle complexe d'épithélium bronchique in vitro en 3D: caractérisation à haut débit de cultures multicellulaires et fabrication de tissus biomimétiques structurés par bio-impression 3D
Moosajee et al. Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells [version 1; peer review: 2 approved]
Li et al. Chemical reprogramming of fibroblasts into retinal pigment epithelium cells for vision restoration
WO2024242069A1 (ja) 神経堤細胞の製造方法
SAVIOJA ATTACHMENT AND DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELL DERIVED RETINAL PIGMENT EPITHELIUM CELLS ON DIFFERENT COATING MATERIALS

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40014216

Country of ref document: HK