JP7246372B2 - 炭水化物センサー - Google Patents

炭水化物センサー Download PDF

Info

Publication number
JP7246372B2
JP7246372B2 JP2020506917A JP2020506917A JP7246372B2 JP 7246372 B2 JP7246372 B2 JP 7246372B2 JP 2020506917 A JP2020506917 A JP 2020506917A JP 2020506917 A JP2020506917 A JP 2020506917A JP 7246372 B2 JP7246372 B2 JP 7246372B2
Authority
JP
Japan
Prior art keywords
seq
lactose
domain
gfp
sensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020506917A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020537489A (ja
JP2020537489A5 (cg-RX-API-DMAC7.html
Inventor
カリーヌ・カロン
スティーブン・チャールズ・トロウェル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2017903148A external-priority patent/AU2017903148A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of JP2020537489A publication Critical patent/JP2020537489A/ja
Publication of JP2020537489A5 publication Critical patent/JP2020537489A5/ja
Application granted granted Critical
Publication of JP7246372B2 publication Critical patent/JP7246372B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/04—Dairy products
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plasma & Fusion (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020506917A 2017-08-08 2018-08-07 炭水化物センサー Active JP7246372B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2017903148A AU2017903148A0 (en) 2017-08-08 Carbohydrate sensors
AU2017903148 2017-08-08
PCT/AU2018/050824 WO2019028504A1 (en) 2017-08-08 2018-08-07 CARBON HYDRATES SENSORS

Publications (3)

Publication Number Publication Date
JP2020537489A JP2020537489A (ja) 2020-12-24
JP2020537489A5 JP2020537489A5 (cg-RX-API-DMAC7.html) 2021-09-16
JP7246372B2 true JP7246372B2 (ja) 2023-03-27

Family

ID=65273001

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020506917A Active JP7246372B2 (ja) 2017-08-08 2018-08-07 炭水化物センサー

Country Status (13)

Country Link
US (1) US11662349B2 (cg-RX-API-DMAC7.html)
EP (1) EP3665481B1 (cg-RX-API-DMAC7.html)
JP (1) JP7246372B2 (cg-RX-API-DMAC7.html)
KR (1) KR102660777B1 (cg-RX-API-DMAC7.html)
CN (1) CN111164426B (cg-RX-API-DMAC7.html)
AR (1) AR112932A1 (cg-RX-API-DMAC7.html)
AU (1) AU2018315053B2 (cg-RX-API-DMAC7.html)
CA (1) CA3071994A1 (cg-RX-API-DMAC7.html)
IL (1) IL272468A (cg-RX-API-DMAC7.html)
PL (1) PL3665481T3 (cg-RX-API-DMAC7.html)
SG (1) SG11202001121WA (cg-RX-API-DMAC7.html)
WO (1) WO2019028504A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA202001115B (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
US11952612B2 (en) 2016-11-14 2024-04-09 Commonwealth Scientific And Industrial Research Organisation Protease sensor molecules
SG11202001121WA (en) 2017-08-08 2020-03-30 Commw Scient Ind Res Org Carbohydrate sensors
CN119955899A (zh) 2018-10-03 2025-05-09 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物、方法和试剂盒
EP4605544A1 (en) * 2022-10-21 2025-08-27 PPB Technology Pty Ltd Method of detecting an analyte in a sample
CN116083465B (zh) * 2023-01-03 2024-01-05 态创生物科技(广州)有限公司 乳糖负传感质粒及负反馈乳糖生物传感器

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000003727A1 (en) 1998-07-17 2000-01-27 University Of Maryland, Baltimore Engineered proteins for analyte sensing
JP2008516607A (ja) 2004-10-14 2008-05-22 カーネギー インスチチューション オブ ワシントン 高感度ｆｒｅｔセンサーの開発およびその使用方法
JP2009520462A (ja) 2005-11-16 2009-05-28 カーネギー インスチチューション オブ ワシントン 多量体バイオセンサーおよびそれを用いる方法
WO2017087912A2 (en) 2015-11-20 2017-05-26 Duke University Ratiometric biosensors and non-geometrically modulated fret

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2191110B (en) 1986-06-06 1989-12-06 Plessey Co Plc Chromatographic separation device
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5196524A (en) 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5580523A (en) 1994-04-01 1996-12-03 Bard; Allen J. Integrated chemical synthesizers
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
US6228604B1 (en) 1996-04-10 2001-05-08 Loma Linda University Secreted renilla luciferase
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP1064360B1 (en) 1998-03-27 2008-03-05 Prolume, Ltd. Luciferases, gfp fluorescent proteins, their nucleic acids and the use thereof in diagnostics
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
EP1194693B1 (en) 1999-06-28 2006-10-25 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US20040214227A1 (en) 1999-12-22 2004-10-28 Erik Joly Bioluminescence resonance energy transfer (bret) fusion molecule and method of use
ATE320000T1 (de) 1999-12-22 2006-03-15 Perkinelmer Biosignal Inc Ein biolumineszenz resonanz energie transfer (bret) system mit breitem spektralen resolution zwischen donator- und akzeptor-emission wellenlänge und ihre anwendung
WO2002010433A2 (en) 2000-05-03 2002-02-07 Expressive Constructs, Inc. Method and device for detecting bacterial contamination
US20030049799A1 (en) * 2000-10-23 2003-03-13 Schwartz John Jacob Engineered stimulus-responsive switches
US6949377B2 (en) 2001-03-05 2005-09-27 Ho Winston Z Chemiluminescence-based microfluidic biochip
WO2003015923A1 (en) 2001-08-20 2003-02-27 Biomicro Systems, Inc. Fluid mixing in low aspect ratio chambers
US7338760B2 (en) 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
AU2003222253A1 (en) 2002-03-05 2003-09-22 Monogram Biosciences, Inc. Multiplex analysis using membrane-bound sensitizers
EP1413584B1 (en) 2002-10-21 2005-03-02 AXXAM S.r.l. Photoprotein with improved bioluminescence
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US7262859B2 (en) 2004-10-13 2007-08-28 U.S. Genomics, Inc. Systems and methods for measurement optimization
WO2006105616A1 (en) 2005-04-08 2006-10-12 Commonwealth Scientific And Industrial Research Organisation Method for microfluidic mixing and mixing device
US8227572B2 (en) 2005-08-19 2012-07-24 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
WO2007033385A2 (en) 2005-09-13 2007-03-22 Fluidigm Corporation Microfluidic assay devices and methods
WO2007092909A2 (en) 2006-02-07 2007-08-16 Expressive Constructs, Inc. Molecular interaction sensors
DE102007002580A1 (de) 2007-01-11 2008-07-17 C-Lecta Gmbh Proteolytische Abspaltung von Protein-Tags in der rekombinanten Protein-Herstellung
US9034402B2 (en) 2007-04-16 2015-05-19 Solae, Llc Protein hydrolysate compositions having improved sensory characteristics and physical properties
EP2149050A2 (en) 2007-04-27 2010-02-03 The Regents Of The University Of California Device and methods for detection of airborne agents
US9714941B2 (en) 2007-07-31 2017-07-25 Massachusetts Institute Of Technology Bio-sensing nanodevice
EP2205357B1 (en) 2007-10-02 2020-07-15 Abbott Rapid Diagnostics International Unlimited Company Assay device and method
JP5737673B2 (ja) 2008-11-10 2015-06-17 国立大学法人名古屋大学 アレルゲンのエピトープ又はその候補の検出方法及びその利用
US8268977B2 (en) 2008-11-20 2012-09-18 The Board Of Trustees Of The Leland Stanford Junior University Strongly quenching oligomeric excimer/quencher pairs for detection schemes
KR101834574B1 (ko) 2009-01-29 2018-03-05 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 Ｇ 단백질 커플링 수용체 활성화 측정
EP2221606A3 (en) 2009-02-11 2012-06-06 Samsung Electronics Co., Ltd. Integrated bio-chip and method of fabricating the integrated bio-chip
US9841426B2 (en) 2009-03-11 2017-12-12 Marker Gene Technologies, Inc Intracellular organelle peptide targeted enzyme substrates
WO2010138794A2 (en) * 2009-05-29 2010-12-02 The University Of North Carolina At Chapel Hill Regulation of sodium channels by plunc proteins
JP2011038922A (ja) 2009-08-12 2011-02-24 Sony Corp 光検出用チップおよび該光検出用チップを用いた光検出装置
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
WO2011091037A2 (en) 2010-01-19 2011-07-28 President And Fellows Of Harvard College Rapid pathogen diagnostic device and method
WO2012034023A2 (en) * 2010-09-10 2012-03-15 University Of Delaware Recombinant clostridia that fix co2 and co and uses thereof
US8828678B2 (en) 2010-11-16 2014-09-09 Enzo Life Sciences, Inc. Self-immolative probes for enzyme activity detection
WO2012149047A1 (en) 2011-04-29 2012-11-01 Sequenom, Inc. Multimer glycosylated nucleic acid binding protein conjugates and uses thereof
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
RU2642794C2 (ru) 2012-07-02 2018-01-26 Дед Си Уоркс Лтд., Погружные плоские устройства для проведения прямого осмоса
US9193990B2 (en) 2013-03-15 2015-11-24 Richard B. Thompson Bioluminescent metal ion assay
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
WO2014207515A1 (en) 2013-06-25 2014-12-31 Tubitak (Turkiye Bilimsel Ve Teknolojik Arastirma Kurumu) Fluorescence method for detecting nuclease activity
EP3022316B1 (en) 2013-07-18 2017-11-29 Ecole Polytechnique Fédérale de Lausanne (EPFL) Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample
ES2899191T3 (es) 2014-04-03 2022-03-10 AllerGenis LLC Péptidos y procedimientos de detección de alergia alimentaria
EP3259365B1 (en) 2015-02-16 2020-04-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Sensors, methods and kits for detecting nicotinamide adenine dinucleotides
SG11202001121WA (en) 2017-08-08 2020-03-30 Commw Scient Ind Res Org Carbohydrate sensors
JP2020531022A (ja) 2017-08-24 2020-11-05 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション ヒドロラーゼを検出するためのｂｒｅｔセンサー分子

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000003727A1 (en) 1998-07-17 2000-01-27 University Of Maryland, Baltimore Engineered proteins for analyte sensing
JP2008516607A (ja) 2004-10-14 2008-05-22 カーネギー インスチチューション オブ ワシントン 高感度ｆｒｅｔセンサーの開発およびその使用方法
JP2009520462A (ja) 2005-11-16 2009-05-28 カーネギー インスチチューション オブ ワシントン 多量体バイオセンサーおよびそれを用いる方法
WO2017087912A2 (en) 2015-11-20 2017-05-26 Duke University Ratiometric biosensors and non-geometrically modulated fret

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Applied and Environmental Microbiology, 2011, Vol.77 No.2 Page.471-478
Scientific Reports, 2017 April, Vol.7, no.45994, DOI: 10.1038/srep45994
The EMBO Journal vol. 1 1 no.8 pp.3031 - 3038, 1992

Also Published As

Publication number Publication date
EP3665481B1 (en) 2023-10-11
CA3071994A1 (en) 2019-02-14
US20200319195A1 (en) 2020-10-08
CN111164426A (zh) 2020-05-15
AU2018315053A1 (en) 2020-02-27
SG11202001121WA (en) 2020-03-30
JP2020537489A (ja) 2020-12-24
EP3665481C0 (en) 2023-10-11
KR102660777B1 (ko) 2024-04-24
AU2018315053B2 (en) 2022-04-28
EP3665481A1 (en) 2020-06-17
ZA202001115B (en) 2021-08-25
IL272468A (en) 2020-03-31
WO2019028504A1 (en) 2019-02-14
EP3665481A4 (en) 2021-08-11
CN111164426B (zh) 2023-12-08
PL3665481T3 (pl) 2024-02-05
US11662349B2 (en) 2023-05-30
AR112932A1 (es) 2020-01-08
KR20200052284A (ko) 2020-05-14

Similar Documents

Publication Publication Date Title
JP7246372B2 (ja) 炭水化物センサー
US20220356508A1 (en) In vitro and cell based assays for measuring the activity of botulinum neurotoxins
AU2017358067B2 (en) Protease sensor molecules
AU2018321580B2 (en) BRET sensor molecules for detecting hydrolases
US20080213796A1 (en) GFP-SNAP25 Fluorescence Release Assay for Botulinum Toxin Protease Activity
CN116287106B (zh) 增强萤火虫萤光素酶atp生物发光法检测性能的方法及试剂
Dacres et al. Comparison of enhanced bioluminescence energy transfer donors for protease biosensors
Gutiérrez-del-Río et al. Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
KR101234583B1 (ko) 형광단백질 ｈｉｓ-ｍＢＦＰ를 이용한 ＮＡＤＰＨ 정량법 및 그의 용도
Goodwin et al. E. coli sabotages the in vivo production of O-linked β-N-acetylglucosamine-modified proteins
Wu et al. A novel screening system based on VanX‐mediated autolysis—Application to Gaussia luciferase
Tate et al. Louis Perdios1, 2, Tom D. Bunney1, Sean C. Warren3, Christopher Dunsby3, Paul MW French3
JP2007325546A (ja) 分割ルシフェラーゼ

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210805

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210805

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220822

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221122

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230213

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230314

R150 Certificate of patent or registration of utility model

Ref document number: 7246372

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150