JP7189866B2 - 新規エステラーゼ及びその使用 - Google Patents

新規エステラーゼ及びその使用 Download PDF

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Publication number
JP7189866B2
JP7189866B2 JP2019501519A JP2019501519A JP7189866B2 JP 7189866 B2 JP7189866 B2 JP 7189866B2 JP 2019501519 A JP2019501519 A JP 2019501519A JP 2019501519 A JP2019501519 A JP 2019501519A JP 7189866 B2 JP7189866 B2 JP 7189866B2
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Japan
Prior art keywords
esterase
polyester
variant
seq
host cell
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Active
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JP2019501519A
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English (en)
Japanese (ja)
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JP2019527060A5 (cg-RX-API-DMAC7.html
JP2019527060A (ja
Inventor
トゥルニエ，ヴァンサン
テキシエ，エレーヌ
デルソー，マリー－ロール
トップハム，クリストファー
アンドレ，イザベル
バルベ，ソフィー
ドゥケーヌ，ソフィー
マルティ，アラン
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Carbios SA
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Carbios SA
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Publication of JP2019527060A5 publication Critical patent/JP2019527060A5/ja
Priority to JP2022069469A priority Critical patent/JP7392029B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01001—Carboxylesterase (3.1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • Detergent Compositions (AREA)
JP2019501519A 2016-07-12 2017-07-12 新規エステラーゼ及びその使用 Active JP7189866B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022069469A JP7392029B2 (ja) 2016-07-12 2022-04-20 新規エステラーゼ及びその使用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16305899 2016-07-12
EP16305899.3 2016-07-12
PCT/EP2017/067577 WO2018011281A1 (en) 2016-07-12 2017-07-12 Novel esterases and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022069469A Division JP7392029B2 (ja) 2016-07-12 2022-04-20 新規エステラーゼ及びその使用

Publications (3)

Publication Number Publication Date
JP2019527060A JP2019527060A (ja) 2019-09-26
JP2019527060A5 JP2019527060A5 (cg-RX-API-DMAC7.html) 2020-07-16
JP7189866B2 true JP7189866B2 (ja) 2022-12-14

Family

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Family Applications (2)

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JP2019501519A Active JP7189866B2 (ja) 2016-07-12 2017-07-12 新規エステラーゼ及びその使用
JP2022069469A Active JP7392029B2 (ja) 2016-07-12 2022-04-20 新規エステラーゼ及びその使用

Family Applications After (1)

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JP2022069469A Active JP7392029B2 (ja) 2016-07-12 2022-04-20 新規エステラーゼ及びその使用

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US (2) US10590401B2 (cg-RX-API-DMAC7.html)
EP (1) EP3485006B1 (cg-RX-API-DMAC7.html)
JP (2) JP7189866B2 (cg-RX-API-DMAC7.html)
KR (2) KR102489444B1 (cg-RX-API-DMAC7.html)
CN (3) CN114891768B (cg-RX-API-DMAC7.html)
CA (1) CA3030469C (cg-RX-API-DMAC7.html)
MX (1) MX2019000493A (cg-RX-API-DMAC7.html)
WO (1) WO2018011281A1 (cg-RX-API-DMAC7.html)

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CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
DE102018210609A1 (de) * 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
EP4424823B1 (en) * 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof
CN112639101B (zh) * 2018-07-27 2025-03-18 卡比奥斯公司 新酯酶及其用途
US11692181B2 (en) * 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
EP3699272A1 (en) 2019-02-21 2020-08-26 Henkel AG & Co. KGaA Improved caring properties on polyester textiles by use of a cutinase
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EP3997220B1 (en) * 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
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JP2023546501A (ja) 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
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US20240228731A1 (en) 2021-05-20 2024-07-11 Carbios Process for degrading a plastic product comprising at least one polyester
CN118994721A (zh) 2021-05-20 2024-11-22 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
JP7732636B2 (ja) * 2021-10-13 2025-09-02 国立大学法人静岡大学 タンパク質、ポリヌクレオチド、組換えベクター、形質転換体、ポリエチレンテレフタレート分解用組成物、及びリサイクル品の製造方法
GB202207298D0 (en) * 2022-05-18 2022-06-29 Cambridge Entpr Ltd Improved plastic degrading enzymes
TW202411323A (zh) 2022-06-13 2024-03-16 法商卡爾畢歐斯公司 純化單乙二醇之方法
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
JPWO2024122208A1 (cg-RX-API-DMAC7.html) * 2022-12-08 2024-06-13
US20240286178A1 (en) 2023-01-09 2024-08-29 Protein Evolution Inc Systems and methods for enzymatic degradation of polymers, including copolymers, with a variety of enzymes
FR3144996A1 (fr) 2023-01-18 2024-07-19 Carbiolice Melange maitre enzyme comprenant des proteines
CN116286727B (zh) * 2023-03-06 2025-09-16 山东大学 一种角质酶变体及其制备方法和在塑料降解中的应用
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KR20260011167A (ko) 2023-05-15 2026-01-22 까르비오 신규 에스테라제 및 이의 용도
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Also Published As

Publication number Publication date
CN114891768A (zh) 2022-08-12
KR102685555B1 (ko) 2024-07-17
CA3030469C (en) 2024-04-30
US10590401B2 (en) 2020-03-17
KR20190028754A (ko) 2019-03-19
US11072784B2 (en) 2021-07-27
CA3030469A1 (en) 2018-01-18
JP7392029B2 (ja) 2023-12-05
EP3485006B1 (en) 2025-10-29
US20200277585A1 (en) 2020-09-03
CN114891766A (zh) 2022-08-12
CN114891768B (zh) 2024-09-24
CN109642221B (zh) 2022-07-08
JP2022101632A (ja) 2022-07-06
US20190225954A1 (en) 2019-07-25
CN109642221A (zh) 2019-04-16
EP3485006A1 (en) 2019-05-22
JP2019527060A (ja) 2019-09-26
EP3485006C0 (en) 2025-10-29
MX2019000493A (es) 2019-04-01
KR20230011505A (ko) 2023-01-20
WO2018011281A1 (en) 2018-01-18
KR102489444B1 (ko) 2023-01-20

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