CN114891768B - 新酯酶及其用途 - Google Patents

新酯酶及其用途 Download PDF

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Publication number
CN114891768B
CN114891768B CN202210724621.9A CN202210724621A CN114891768B CN 114891768 B CN114891768 B CN 114891768B CN 202210724621 A CN202210724621 A CN 202210724621A CN 114891768 B CN114891768 B CN 114891768B
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China
Prior art keywords
esterase
polyester
host cell
activity
seq
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CN202210724621.9A
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Chinese (zh)
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CN114891768A (zh
Inventor
V·图尼尔
H·特谢尔
M-L·德鲁索
C·托珀姆
I·安德烈
S·巴比
S·迪奎斯内
A·马蒂
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Carbios SA
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Carbios SA
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Priority to CN202210724621.9A priority Critical patent/CN114891768B/zh
Publication of CN114891768A publication Critical patent/CN114891768A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01001—Carboxylesterase (3.1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • Detergent Compositions (AREA)
CN202210724621.9A 2016-07-12 2017-07-12 新酯酶及其用途 Active CN114891768B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210724621.9A CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP16305899 2016-07-12
EP16305899.3 2016-07-12
CN201780051442.0A CN109642221B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210724621.9A CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
PCT/EP2017/067577 WO2018011281A1 (en) 2016-07-12 2017-07-12 Novel esterases and uses thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201780051442.0A Division CN109642221B (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Publications (2)

Publication Number Publication Date
CN114891768A CN114891768A (zh) 2022-08-12
CN114891768B true CN114891768B (zh) 2024-09-24

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CN202210724621.9A Active CN114891768B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN201780051442.0A Active CN109642221B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A Pending CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

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CN201780051442.0A Active CN109642221B (zh) 2016-07-12 2017-07-12 新酯酶及其用途
CN202210723659.4A Pending CN114891766A (zh) 2016-07-12 2017-07-12 新酯酶及其用途

Country Status (8)

Country Link
US (2) US10590401B2 (cg-RX-API-DMAC7.html)
EP (1) EP3485006B1 (cg-RX-API-DMAC7.html)
JP (2) JP7189866B2 (cg-RX-API-DMAC7.html)
KR (2) KR102489444B1 (cg-RX-API-DMAC7.html)
CN (3) CN114891768B (cg-RX-API-DMAC7.html)
CA (1) CA3030469C (cg-RX-API-DMAC7.html)
MX (1) MX2019000493A (cg-RX-API-DMAC7.html)
WO (1) WO2018011281A1 (cg-RX-API-DMAC7.html)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN116286728B (zh) 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN114891768B (zh) * 2016-07-12 2024-09-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
DE102018210609A1 (de) * 2018-06-28 2020-01-02 Henkel Ag & Co. Kgaa Polyesterase II
EP4424823B1 (en) * 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof
CN112639101B (zh) * 2018-07-27 2025-03-18 卡比奥斯公司 新酯酶及其用途
US11692181B2 (en) * 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
EP3699272A1 (en) 2019-02-21 2020-08-26 Henkel AG & Co. KGaA Improved caring properties on polyester textiles by use of a cutinase
US12492308B2 (en) 2019-03-28 2025-12-09 Carbios Multicomponent thermoplastic product
CN114096664B (zh) * 2019-07-11 2025-03-07 卡比奥斯公司 新型酯酶及其用途
EP3997220B1 (en) * 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
AU2020409729A1 (en) 2019-12-19 2022-06-16 Carbios Process for degrading plastic products
WO2021123328A1 (en) 2019-12-19 2021-06-24 Carbios Foamed plastic compositions
CN111100835B (zh) * 2020-01-07 2021-12-31 中国科学院青岛生物能源与过程研究所 Pet降解生物催化剂及其应用
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
JP2023546501A (ja) 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
US20230399628A1 (en) * 2020-10-27 2023-12-14 Carbios Novel esterases and uses thereof
JP2023546500A (ja) * 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
US20240228731A1 (en) 2021-05-20 2024-07-11 Carbios Process for degrading a plastic product comprising at least one polyester
CN118994721A (zh) 2021-05-20 2024-11-22 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
JP7732636B2 (ja) * 2021-10-13 2025-09-02 国立大学法人静岡大学 タンパク質、ポリヌクレオチド、組換えベクター、形質転換体、ポリエチレンテレフタレート分解用組成物、及びリサイクル品の製造方法
GB202207298D0 (en) * 2022-05-18 2022-06-29 Cambridge Entpr Ltd Improved plastic degrading enzymes
TW202411323A (zh) 2022-06-13 2024-03-16 法商卡爾畢歐斯公司 純化單乙二醇之方法
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
JPWO2024122208A1 (cg-RX-API-DMAC7.html) * 2022-12-08 2024-06-13
US20240286178A1 (en) 2023-01-09 2024-08-29 Protein Evolution Inc Systems and methods for enzymatic degradation of polymers, including copolymers, with a variety of enzymes
FR3144996A1 (fr) 2023-01-18 2024-07-19 Carbiolice Melange maitre enzyme comprenant des proteines
CN116286727B (zh) * 2023-03-06 2025-09-16 山东大学 一种角质酶变体及其制备方法和在塑料降解中的应用
US20240352064A1 (en) 2023-03-21 2024-10-24 Protein Evolution Inc. Methods and compositions for the separation of particles from a fluid
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
KR20260011167A (ko) 2023-05-15 2026-01-22 까르비오 신규 에스테라제 및 이의 용도
EP4743509A2 (en) 2023-07-10 2026-05-20 Carbios TEXTILE WASTE PRE-TREATMENT PROCESS
WO2025206321A1 (ja) * 2024-03-29 2025-10-02 東洋紡株式会社 Ｐｅｆの酵素分解
GB202405531D0 (en) * 2024-04-19 2024-06-05 Prontonera Ltd Process
CN122256301A (zh) * 2025-09-28 2026-06-23 源天生物科技(天津)有限公司 BhrPETase突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2694537A1 (en) * 2011-04-08 2014-02-12 Danisco US Inc. Compositions
FR2988733B1 (fr) 2012-03-27 2016-02-05 Carbios Microorganisme recombinant
EP2861615A1 (en) 2012-06-04 2015-04-22 Carbios Recombinant cellulosome complex and uses thereof
TR201900782T4 (tr) 2012-11-20 2019-02-21 Carbios Plastik ürünlerin geri dönüştürülmesine yönelik yöntem.
US9434940B2 (en) * 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US20160280881A1 (en) 2013-11-05 2016-09-29 Carbios A method for degrading a plastic
JP6315978B2 (ja) * 2013-12-24 2018-04-25 合同会社ミクロバイオ開発研究所 新規なクチナーゼ、クチナーゼをコードする遺伝子、及びクチナーゼを用いたポリエステル又はエステル化合物の分解方法
ES2924896T3 (es) 2014-05-16 2022-10-11 Carbios Procedimiento para reciclar artículos de plástico mixto con PET
EP3778883A1 (en) * 2014-10-21 2021-02-17 Carbios Polypeptide having a polyester degrading activity and uses thereof
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
EP3394264A1 (en) 2015-12-21 2018-10-31 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CN105400750B (zh) * 2015-12-28 2018-08-17 国家海洋局第二海洋研究所 一种深海新型低温耐盐酯酶及应用
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN116286728B (zh) 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN114891768B (zh) * 2016-07-12 2024-09-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ

Also Published As

Publication number Publication date
CN114891768A (zh) 2022-08-12
KR102685555B1 (ko) 2024-07-17
CA3030469C (en) 2024-04-30
US10590401B2 (en) 2020-03-17
JP7189866B2 (ja) 2022-12-14
KR20190028754A (ko) 2019-03-19
US11072784B2 (en) 2021-07-27
CA3030469A1 (en) 2018-01-18
JP7392029B2 (ja) 2023-12-05
EP3485006B1 (en) 2025-10-29
US20200277585A1 (en) 2020-09-03
CN114891766A (zh) 2022-08-12
CN109642221B (zh) 2022-07-08
JP2022101632A (ja) 2022-07-06
US20190225954A1 (en) 2019-07-25
CN109642221A (zh) 2019-04-16
EP3485006A1 (en) 2019-05-22
JP2019527060A (ja) 2019-09-26
EP3485006C0 (en) 2025-10-29
MX2019000493A (es) 2019-04-01
KR20230011505A (ko) 2023-01-20
WO2018011281A1 (en) 2018-01-18
KR102489444B1 (ko) 2023-01-20

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