JP7122009B2 - 産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド - Google Patents

産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド Download PDF

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JP7122009B2
JP7122009B2 JP2019568657A JP2019568657A JP7122009B2 JP 7122009 B2 JP7122009 B2 JP 7122009B2 JP 2019568657 A JP2019568657 A JP 2019568657A JP 2019568657 A JP2019568657 A JP 2019568657A JP 7122009 B2 JP7122009 B2 JP 7122009B2
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seq
amino acid
transaminase
sequence
polypeptide
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JP2020523023A (ja
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スコット ジェイ． ノヴィック，
ニッキ デラス，
オスカー アルビゾ，
ラヴィ デイビッド ガルシア，
シャーリーン チン，
デイビッド エントウィッスル，
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コデクシス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019568657A 2017-06-14 2018-05-23 産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド Active JP7122009B2 (ja)

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JP2022010034A JP7212972B2 (ja) 2017-06-14 2022-01-26 産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド

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US201762519297P 2017-06-14 2017-06-14
US62/519,297 2017-06-14
PCT/US2018/034069 WO2018231462A1 (en) 2017-06-14 2018-05-23 Engineered transaminase polypeptides for industrial biocatalysis

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JP2022010034A Active JP7212972B2 (ja) 2017-06-14 2022-01-26 産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド

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US (3) US11359183B2 (enExample)
EP (1) EP3638688A4 (enExample)
JP (2) JP7122009B2 (enExample)
CN (1) CN111032682B (enExample)
CA (1) CA3064574A1 (enExample)
IL (1) IL271114A (enExample)
WO (1) WO2018231462A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109456952B (zh) * 2018-11-30 2020-08-04 江南大学 一种可催化西他沙星五元环关键中间体的ω-转氨酶突变体
JP2023521772A (ja) * 2020-04-10 2023-05-25 コデクシス， インコーポレイテッド 操作されたトランスアミナーゼポリペプチド
CN112094856B (zh) * 2020-09-23 2023-06-27 国药集团威奇达药业有限公司 一种转氨酶突变体及其在西格列汀合成中的应用
CA3193755A1 (en) * 2020-09-28 2022-03-31 Codexis, Inc. Engineered biocatalysts and methods for synthesizing chiral amines
CN112430632B (zh) * 2020-11-18 2022-09-13 上海合全药物研发有限公司 2-((反式)-4-氨基环己烷基)异丙醇的制备方法
CN114645027B (zh) * 2020-12-21 2024-12-10 上海合全药物研发有限公司 一种来源于巨大芽孢杆菌的氨基转移酶突变体及其应用
CN112501145A (zh) * 2021-02-04 2021-03-16 凯莱英医药集团（天津）股份有限公司 转氨酶突变体及其应用
CN113481254B (zh) * 2021-06-29 2023-05-26 台州酶易生物技术有限公司 西他列汀中间体的制备方法
JP2024534364A (ja) * 2021-09-15 2024-09-20 リヒター ゲデオン ニュイルヴァーノシャン ミューコェデー レースヴェーニュタールシャシャーグ （１ｒ，４ｒ）－４－置換シクロヘキサン－１－アミンを生成する方法
CN115851649B (zh) * 2022-04-21 2024-12-03 凯莱英医药集团（天津）股份有限公司 转氨酶突变体及其应用
CN115807046A (zh) * 2022-09-13 2023-03-17 华中科技大学 一种转氨酶催化合成卡巴拉汀药物中间体的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010081053A2 (en) 2009-01-08 2010-07-15 Codexis, Inc. Transaminase polypeptides
JP2016501544A (ja) 2012-12-21 2016-01-21 コデクシス， インコーポレイテッド キラルアミンを合成するための操作された生体触媒および方法
JP2016516744A (ja) 2013-03-27 2016-06-09 ファイザー アイルランド ファーマシューティカルズ プレガバリンの調製のための方法および中間体

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4600692A (en) 1983-02-10 1986-07-15 Purification Engineering, Inc. Immobilized cells for preparing phenylalanine
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4518692A (en) 1983-09-01 1985-05-21 Genetics Institute, Inc. Production of L-amino acids by transamination
US4826766A (en) 1985-09-23 1989-05-02 Genetics Institute, Inc. Production of amino acids using coupled aminotransferases
US5316943A (en) 1988-06-14 1994-05-31 Kidman Gene E Racemic conversion of using a transaminase
US5169780A (en) 1989-06-22 1992-12-08 Celgene Corporation Enantiomeric enrichment and stereoselective synthesis of chiral amines
US5300437A (en) 1989-06-22 1994-04-05 Celgene Corporation Enantiomeric enrichment and stereoselective synthesis of chiral amines
US4950606A (en) 1989-06-22 1990-08-21 Celgene Corporation Enantiomeric enrichment and stereoselective synthesis of chiral amines
JPH06510023A (ja) * 1991-04-18 1994-11-10 ユニバーシティ　オブ　ジョージア　リサーチ　ファウンデーション，インコーポレイテッド キヌレニナーゼの阻害剤
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
KR970703426A (ko) 1994-06-03 1997-07-03 제임스 쉐한 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME)
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO1998031816A1 (en) 1997-01-17 1998-07-23 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69841075D1 (de) 1997-04-23 2009-10-01 Kaneka Corp Deoxynukleinsäure welche ein Polypeptid mit stereoselektiver Transaminase Aktivität kodiert, sowie die Deoxynukleinsäure enthaltende Transformanden
US6197558B1 (en) 1997-05-19 2001-03-06 Nsc Technologies Transaminase biotransformation process
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
KR20010034731A (ko) 1998-04-02 2001-04-25 텔루스 제너틱 리소시즈, 인크. 유전자 서열 중에 유전자 병변이 있는 식물을 얻는 방법
EP1073670A1 (en) 1998-05-01 2001-02-07 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
AU4441699A (en) 1998-06-17 2000-01-05 Maxygen, Inc. Method for producing polynucleotides with desired properties
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6605430B1 (en) 1998-08-12 2003-08-12 Maxygen, Inc. DNA shuffling of monooxygenase genes for production of industrial chemicals
CN1230546C (zh) 1998-10-06 2005-12-07 马克·阿龙·埃马尔法尔布 丝状真菌宿主领域的转化系统
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
IL137868A0 (en) 1999-01-19 2001-10-31 Maxygen Inc Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
AU3719500A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6703240B1 (en) 1999-04-13 2004-03-09 Maxygar, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
SG121902A1 (en) 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
WO2001075767A2 (en) 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
ATE500323T1 (de) 2000-04-03 2011-03-15 Maxygen Inc Subtilisin-variante
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
ES2564570T3 (es) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Métodos, sistemas y software para la identificación de biomoléculas funcionales
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
US20050009151A1 (en) 2003-07-10 2005-01-13 Pharmacia Corporation Methods for the stereospecific and enantiomeric enrichment of beta-amino acids
WO2005017135A1 (en) 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
EP2118281A2 (en) 2007-02-12 2009-11-18 Codexis, Inc. Structure-activity relationships
WO2008127646A2 (en) 2007-04-11 2008-10-23 Dsm Ip Assets B.V. Transaminase-based processes for preparation of pregabalin
EP2250594B1 (en) 2008-02-12 2017-04-19 Codexis, Inc. Method of generating an optimized, diverse population of variants
US8504498B2 (en) 2008-02-12 2013-08-06 Codexis, Inc. Method of generating an optimized, diverse population of variants
ES2575005T3 (es) 2008-06-13 2016-06-23 Codexis, Inc. Método de síntesis de variantes de polinucleótidos
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
CN102405281B (zh) 2009-02-26 2015-05-13 科德克希思公司 转氨酶生物催化剂
US8921079B2 (en) 2009-06-22 2014-12-30 Codexis, Inc. Transaminase reactions
EP2462115B1 (en) 2009-08-05 2016-01-06 Infinity Pharmaceuticals, Inc. Enzymatic transamination of cyclopamine analogs
NZ628085A (en) * 2009-11-07 2015-12-24 Astute Medical Inc Methods and compositions for diagnosis and prognosis of renal injury and renal failure
US9593326B2 (en) 2009-12-09 2017-03-14 Codexis, Inc. Protein variant generation by region shuffling
EP2582799B1 (en) 2010-06-17 2017-12-20 Codexis, Inc. Biocatalysts and methods for the synthesis of (s)-3-(1-aminoethyl)-phenol
US8932836B2 (en) 2010-08-16 2015-01-13 Codexis, Inc. Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
US20120141420A1 (en) * 2010-11-30 2012-06-07 Sygnis Bioscience Gmbh & Co. Kg Targeted delivery of g-csf for the treatment of amyotrophic lateral sclerosis
CA2838744C (en) 2011-06-24 2020-10-13 Merck Sharp & Dohme Corp. Immobilized transaminases and process for making and using immobilized transaminase
DK2828385T3 (en) * 2012-03-23 2018-03-12 Codexis Inc BIOCATALIZATORS AND METHODS FOR SYNTHETIZING DERIVATIVES AND TRYPTAMIN AND TRYPTAMINE ANALOGS
US20150133698A1 (en) 2012-04-20 2015-05-14 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
SG11201506748WA (en) 2013-02-28 2015-09-29 Codexis Inc Engineered transaminase polypeptides for industrial biocatalysis
KR20160026977A (ko) 2013-06-12 2016-03-09 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 트랜스아미나제 생물촉매
BR112017020561B1 (pt) 2015-04-16 2024-01-16 F. Hoffmann-La Roche Ag Transaminase mutante, proteína de fusão, método de produção de amina e uso da transaminase mutante
JO3771B1 (ar) * 2015-12-10 2021-01-31 Novartis Ag متوسطات لتحضير ساكوبيتريل وتحضيرها
EP3510170B1 (en) * 2016-09-12 2021-10-13 Technische Hochschule Wildau Convertible adapters

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010081053A2 (en) 2009-01-08 2010-07-15 Codexis, Inc. Transaminase polypeptides
JP2016501544A (ja) 2012-12-21 2016-01-21 コデクシス， インコーポレイテッド キラルアミンを合成するための操作された生体触媒および方法
JP2016516744A (ja) 2013-03-27 2016-06-09 ファイザー アイルランド ファーマシューティカルズ プレガバリンの調製のための方法および中間体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ACS Catalysis，2016年10月04日，Vol.6，pp.7749-7759

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CN111032682B (zh) 2024-04-12
JP2020523023A (ja) 2020-08-06
IL271114A (en) 2020-01-30
US12297462B2 (en) 2025-05-13
US20200109378A1 (en) 2020-04-09
US20220282229A1 (en) 2022-09-08
EP3638688A1 (en) 2020-04-22
EP3638688A4 (en) 2021-07-07
US11359183B2 (en) 2022-06-14
WO2018231462A1 (en) 2018-12-20
CA3064574A1 (en) 2018-12-20
US20250250550A1 (en) 2025-08-07
JP7212972B2 (ja) 2023-01-26
JP2022062113A (ja) 2022-04-19
CN111032682A (zh) 2020-04-17

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