JP7089582B2 - 新規なプロモーター及びこれを用いたｌ－アミノ酸の生産方法 - Google Patents

新規なプロモーター及びこれを用いたｌ－アミノ酸の生産方法 Download PDF

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Publication number
JP7089582B2
JP7089582B2 JP2020512734A JP2020512734A JP7089582B2 JP 7089582 B2 JP7089582 B2 JP 7089582B2 JP 2020512734 A JP2020512734 A JP 2020512734A JP 2020512734 A JP2020512734 A JP 2020512734A JP 7089582 B2 JP7089582 B2 JP 7089582B2
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polynucleotide
seq
hisg
gene
microorganism
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JP2021517802A (ja
JP2021517802A5 (https=
Inventor
ヨン リ，チ
ソク チャン，チン
チュン キム，ヒョン
フン ユン，ピョン
ヒョン チェ，ソン
チェ，ヨンチョン
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CJ CheilJedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02017—ATP phosphoribosyltransferase (2.4.2.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01031—Phosphoribosyl-ATP diphosphatase (3.6.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020512734A 2018-03-27 2019-03-27 新規なプロモーター及びこれを用いたｌ－アミノ酸の生産方法 Active JP7089582B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2018-0035156 2018-03-27
KR20180035156 2018-03-27
PCT/KR2019/003565 WO2019190192A1 (ko) 2018-03-27 2019-03-27 신규한 프로모터 및 이를 이용한 l-아미노산 생산 방법

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JP2021517802A JP2021517802A (ja) 2021-07-29
JP2021517802A5 JP2021517802A5 (https=) 2021-09-16
JP7089582B2 true JP7089582B2 (ja) 2022-06-22

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JP2020512734A Active JP7089582B2 (ja) 2018-03-27 2019-03-27 新規なプロモーター及びこれを用いたｌ－アミノ酸の生産方法
JP2020512742A Active JP7125477B2 (ja) 2018-03-27 2019-03-27 グリシン生産能が増加された微生物及びこれを用いた発酵組成物の生産方法
JP2022076489A Active JP7607613B2 (ja) 2018-03-27 2022-05-06 グリシン生産能が増加された微生物及びこれを用いた発酵組成物の生産方法

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JP2022076489A Active JP7607613B2 (ja) 2018-03-27 2022-05-06 グリシン生産能が増加された微生物及びこれを用いた発酵組成物の生産方法

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US (2) US10961554B2 (https=)
EP (3) EP3778901B1 (https=)
JP (3) JP7089582B2 (https=)
KR (3) KR102137806B1 (https=)
CN (2) CN111433366B (https=)
AR (2) AR115302A1 (https=)
AU (2) AU2019243242B2 (https=)
BR (2) BR112020003867B1 (https=)
CA (2) CA3073177C (https=)
MY (1) MY202021A (https=)
SG (2) SG11202001730TA (https=)
TW (3) TWI716841B (https=)
WO (2) WO2019190193A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI716841B (zh) * 2018-03-27 2021-01-21 南韓商Ｃｊ第一製糖股份有限公司 新穎啟動子及使用該啟動子製造l-胺基酸的方法
KR102433200B1 (ko) * 2020-12-28 2022-08-19 대상 주식회사 히스티딘에 의한 피드백 억제가 감소된 atp-prt 변이체 및 이를 발현하는 히스티딘 생산 균주
KR102808403B1 (ko) * 2020-12-28 2025-05-20 대상 주식회사 히스티딘에 의한 피드백 억제가 감소된 atp-prt 변이체 및 이를 발현하는 히스티딘 생산 균주
KR102281360B1 (ko) * 2021-01-26 2021-07-22 씨제이제일제당 (주) 신규한 atp 포스포리보실트랜스퍼라제 변이체 및 이를 이용한 l-발린 생산 방법
CN113994003B (zh) * 2021-05-07 2022-10-14 Cj第一制糖株式会社 新型启动子及其用途
KR102377745B1 (ko) * 2021-05-12 2022-03-23 씨제이제일제당 주식회사 신규 프로모터 및 이의 용도
JP7422888B2 (ja) * 2021-05-20 2024-01-26 シージェイ チェイルジェダン コーポレーション 新規なプロモーター及びその用途
JPWO2023013655A1 (https=) * 2021-08-02 2023-02-09
CN116254242B (zh) * 2022-12-21 2024-01-30 江南大学 一种atp磷酸核苷转移酶突变体及产l-组氨酸的谷氨酸棒杆菌
KR20240108882A (ko) * 2022-12-30 2024-07-10 씨제이제일제당 (주) 신규한 폴리뉴클레오티드 및 이를 이용한 l-알라닌 생산 방법
KR20240142905A (ko) * 2023-03-22 2024-10-02 씨제이제일제당 (주) 글리신 생산능이 향상된 미생물 및 이를 이용한 글리신 생산 방법
WO2026019219A1 (ko) * 2024-07-16 2026-01-22 씨제이제일제당 (주) 폴리뉴클레오타이드 변이체 및 이를 이용한 l-히스티딘 생산 방법

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3220929A (en) 1964-02-10 1965-11-30 Kyowa Hakko Kogyo Kk Microbiological production of amino acid by reductive amination
AU746542B2 (en) 1998-03-18 2002-05-02 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
KR100292299B1 (ko) 1999-03-22 2001-06-01 손경식 글루탐산 생산 미생물 및 이를 이용한 글루탐산 생산방법
US6984512B1 (en) * 1999-08-02 2006-01-10 Archer-Daniels-Midland Company Bacterial strains, methods of preparing the same and use thereof in fermentation processes for L-lysine production
CA2380613C (en) * 1999-08-02 2014-03-25 Archer-Daniels-Midland Company Production of l-lysine by corynebacterium strain having mutation in ilvbn operon
DE10044831A1 (de) * 2000-03-01 2002-04-04 Forschungszentrum Juelich Gmbh Verbessertes Verfahren zur mikrobiellen Herstellung von L-Serin sowie ein dazu geeigneter genetisch veränderter Mikroorganismus
WO2005010175A1 (en) 2003-07-29 2005-02-03 Ajinomoto Co., Inc. Method for producing l-lysine or l-threonine using escherichia bacteria having attnuated malic enzyme activity
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
WO2008033001A1 (en) 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR101492134B1 (ko) 2012-05-22 2015-02-12 백광산업 주식회사 코리네형 세균 유래 ｓｏｄ 유전자의 프로모터 변이체 및 상기 프로모터 변이체를 이용한 Ｌ-라이신의 생산 방법
DE102012016716A1 (de) * 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
JP6519476B2 (ja) * 2013-10-23 2019-05-29 味の素株式会社 目的物質の製造法
CN104845923B (zh) * 2014-02-14 2018-03-23 中国科学院微生物研究所 生产l‑组氨酸的方法及其专用重组菌
KR20160072278A (ko) 2014-12-12 2016-06-23 한국외국어대학교 연구산학협력단 합성 글루탐산 탈수소효소 프로모터를 이용한 목적단백질의 생산 방법
KR101904666B1 (ko) * 2017-08-02 2018-11-29 씨제이제일제당 (주) Atp 포스포리보실기 전이효소 변이체 및 이를 이용한 l-히스티딘 생산방법
TWI716841B (zh) * 2018-03-27 2021-01-21 南韓商Ｃｊ第一製糖股份有限公司 新穎啟動子及使用該啟動子製造l-胺基酸的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Definition: Corynebacterium glutamicum strain HA chrmosome, complete genome.，Database GenBank [online]，2017年12月27日，<https://www.ncbi.nlm.nih.gov/nuccore/CP025534>，Accession No.CP025534.1

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Publication number Publication date
EP3778900A1 (en) 2021-02-17
US11661616B2 (en) 2023-05-30
KR102150041B1 (ko) 2020-08-31
AU2019243241A1 (en) 2020-03-12
AU2019243242B2 (en) 2023-04-13
TWI746953B (zh) 2021-11-21
CA3073177C (en) 2022-12-13
EP3778901B1 (en) 2026-05-06
BR112020003467A2 (pt) 2020-08-25
SG11202001753TA (en) 2020-03-30
TW201942360A (zh) 2019-11-01
US10961554B2 (en) 2021-03-30
SG11202001730TA (en) 2020-03-30
AR115301A1 (es) 2020-12-23
AU2019243242A1 (en) 2020-03-12
WO2019190192A1 (ko) 2019-10-03
CA3073180C (en) 2024-03-12
BR112020003867A2 (pt) 2020-10-27
US20200248217A1 (en) 2020-08-06
KR102028554B1 (ko) 2019-10-04
JP2021517802A (ja) 2021-07-29
TW202117002A (zh) 2021-05-01
EP4130281A1 (en) 2023-02-08
CA3073180A1 (en) 2019-10-03
CA3073177A1 (en) 2019-10-03
BR112020003467B8 (pt) 2024-01-16
CN111433366B (zh) 2024-01-09
BR112020003467B1 (pt) 2023-08-15
BR112020003867B1 (pt) 2022-04-12
JP2021515537A (ja) 2021-06-24
KR102137806B1 (ko) 2020-07-27
AR115302A1 (es) 2020-12-23
CN111655860A (zh) 2020-09-11
JP2022105171A (ja) 2022-07-12
EP3778901A1 (en) 2021-02-17
WO2019190193A1 (ko) 2019-10-03
CN111433366A (zh) 2020-07-17
TW201945535A (zh) 2019-12-01
JP7125477B2 (ja) 2022-08-24
KR20190113649A (ko) 2019-10-08
JP7607613B2 (ja) 2024-12-27
US20210002655A1 (en) 2021-01-07
CN111655860B (zh) 2023-10-03
TWI716841B (zh) 2021-01-21
EP3778900A4 (en) 2021-12-22
MY202021A (en) 2024-03-29
EP3778901A4 (en) 2021-12-22
KR20200090693A (ko) 2020-07-29
AU2019243241B2 (en) 2022-12-01

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