CA3073177C - A novel promoter and a method for producing l-amino acid using the same - Google Patents

A novel promoter and a method for producing l-amino acid using the same Download PDF

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Publication number
CA3073177C
CA3073177C CA3073177A CA3073177A CA3073177C CA 3073177 C CA3073177 C CA 3073177C CA 3073177 A CA3073177 A CA 3073177A CA 3073177 A CA3073177 A CA 3073177A CA 3073177 C CA3073177 C CA 3073177C
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polynucleotide
hisg
microorganism
gene
atcc13869
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CA3073177A1 (en
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Ji Yeon Lee
Jin Sook Chang
Hyung Joon Kim
Byoung Hoon Yoon
Sun Hyoung Choi
Yunjung Choi
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CJ CheilJedang Corp
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1077Pentosyltransferases (2.4.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/14Glutamic acid; Glutamine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/02Pentosyltransferases (2.4.2)
    • C12Y204/02017ATP phosphoribosyltransferase (2.4.2.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y306/00Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01031Phosphoribosyl-ATP diphosphatase (3.6.1.31)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/55Vector systems having a special element relevant for transcription from bacteria

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3073177A 2018-03-27 2019-03-27 A novel promoter and a method for producing l-amino acid using the same Active CA3073177C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2018-0035156 2018-03-27
KR20180035156 2018-03-27
PCT/KR2019/003565 WO2019190192A1 (ko) 2018-03-27 2019-03-27 신규한 프로모터 및 이를 이용한 l-아미노산 생산 방법

Publications (2)

Publication Number Publication Date
CA3073177A1 CA3073177A1 (en) 2019-10-03
CA3073177C true CA3073177C (en) 2022-12-13

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Family Applications (2)

Application Number Title Priority Date Filing Date
CA3073177A Active CA3073177C (en) 2018-03-27 2019-03-27 A novel promoter and a method for producing l-amino acid using the same
CA3073180A Active CA3073180C (en) 2018-03-27 2019-03-27 Microorganism having increased glycine productivity and method for producing fermented composition using the same

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3073180A Active CA3073180C (en) 2018-03-27 2019-03-27 Microorganism having increased glycine productivity and method for producing fermented composition using the same

Country Status (13)

Country Link
US (2) US10961554B2 (https=)
EP (3) EP3778901B1 (https=)
JP (3) JP7089582B2 (https=)
KR (3) KR102137806B1 (https=)
CN (2) CN111433366B (https=)
AR (2) AR115302A1 (https=)
AU (2) AU2019243242B2 (https=)
BR (2) BR112020003867B1 (https=)
CA (2) CA3073177C (https=)
MY (1) MY202021A (https=)
SG (2) SG11202001730TA (https=)
TW (3) TWI716841B (https=)
WO (2) WO2019190193A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI716841B (zh) * 2018-03-27 2021-01-21 南韓商Cj第一製糖股份有限公司 新穎啟動子及使用該啟動子製造l-胺基酸的方法
KR102433200B1 (ko) * 2020-12-28 2022-08-19 대상 주식회사 히스티딘에 의한 피드백 억제가 감소된 atp-prt 변이체 및 이를 발현하는 히스티딘 생산 균주
KR102808403B1 (ko) * 2020-12-28 2025-05-20 대상 주식회사 히스티딘에 의한 피드백 억제가 감소된 atp-prt 변이체 및 이를 발현하는 히스티딘 생산 균주
KR102281360B1 (ko) * 2021-01-26 2021-07-22 씨제이제일제당 (주) 신규한 atp 포스포리보실트랜스퍼라제 변이체 및 이를 이용한 l-발린 생산 방법
CN113994003B (zh) * 2021-05-07 2022-10-14 Cj第一制糖株式会社 新型启动子及其用途
KR102377745B1 (ko) * 2021-05-12 2022-03-23 씨제이제일제당 주식회사 신규 프로모터 및 이의 용도
JP7422888B2 (ja) * 2021-05-20 2024-01-26 シージェイ チェイルジェダン コーポレーション 新規なプロモーター及びその用途
JPWO2023013655A1 (https=) * 2021-08-02 2023-02-09
CN116254242B (zh) * 2022-12-21 2024-01-30 江南大学 一种atp磷酸核苷转移酶突变体及产l-组氨酸的谷氨酸棒杆菌
KR20240108882A (ko) * 2022-12-30 2024-07-10 씨제이제일제당 (주) 신규한 폴리뉴클레오티드 및 이를 이용한 l-알라닌 생산 방법
KR20240142905A (ko) * 2023-03-22 2024-10-02 씨제이제일제당 (주) 글리신 생산능이 향상된 미생물 및 이를 이용한 글리신 생산 방법
WO2026019219A1 (ko) * 2024-07-16 2026-01-22 씨제이제일제당 (주) 폴리뉴클레오타이드 변이체 및 이를 이용한 l-히스티딘 생산 방법

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
US3220929A (en) 1964-02-10 1965-11-30 Kyowa Hakko Kogyo Kk Microbiological production of amino acid by reductive amination
AU746542B2 (en) 1998-03-18 2002-05-02 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
KR100292299B1 (ko) 1999-03-22 2001-06-01 손경식 글루탐산 생산 미생물 및 이를 이용한 글루탐산 생산방법
US6984512B1 (en) * 1999-08-02 2006-01-10 Archer-Daniels-Midland Company Bacterial strains, methods of preparing the same and use thereof in fermentation processes for L-lysine production
CA2380613C (en) * 1999-08-02 2014-03-25 Archer-Daniels-Midland Company Production of l-lysine by corynebacterium strain having mutation in ilvbn operon
DE10044831A1 (de) * 2000-03-01 2002-04-04 Forschungszentrum Juelich Gmbh Verbessertes Verfahren zur mikrobiellen Herstellung von L-Serin sowie ein dazu geeigneter genetisch veränderter Mikroorganismus
WO2005010175A1 (en) 2003-07-29 2005-02-03 Ajinomoto Co., Inc. Method for producing l-lysine or l-threonine using escherichia bacteria having attnuated malic enzyme activity
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc L−アミノ酸を生産する微生物及びl−アミノ酸の製造法
WO2008033001A1 (en) 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR101492134B1 (ko) 2012-05-22 2015-02-12 백광산업 주식회사 코리네형 세균 유래 sod 유전자의 프로모터 변이체 및 상기 프로모터 변이체를 이용한 L-라이신의 생산 방법
DE102012016716A1 (de) * 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
JP6519476B2 (ja) * 2013-10-23 2019-05-29 味の素株式会社 目的物質の製造法
CN104845923B (zh) * 2014-02-14 2018-03-23 中国科学院微生物研究所 生产l‑组氨酸的方法及其专用重组菌
KR20160072278A (ko) 2014-12-12 2016-06-23 한국외국어대학교 연구산학협력단 합성 글루탐산 탈수소효소 프로모터를 이용한 목적단백질의 생산 방법
KR101904666B1 (ko) * 2017-08-02 2018-11-29 씨제이제일제당 (주) Atp 포스포리보실기 전이효소 변이체 및 이를 이용한 l-히스티딘 생산방법
TWI716841B (zh) * 2018-03-27 2021-01-21 南韓商Cj第一製糖股份有限公司 新穎啟動子及使用該啟動子製造l-胺基酸的方法

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Publication number Publication date
EP3778900A1 (en) 2021-02-17
US11661616B2 (en) 2023-05-30
KR102150041B1 (ko) 2020-08-31
AU2019243241A1 (en) 2020-03-12
AU2019243242B2 (en) 2023-04-13
TWI746953B (zh) 2021-11-21
EP3778901B1 (en) 2026-05-06
BR112020003467A2 (pt) 2020-08-25
SG11202001753TA (en) 2020-03-30
TW201942360A (zh) 2019-11-01
US10961554B2 (en) 2021-03-30
SG11202001730TA (en) 2020-03-30
AR115301A1 (es) 2020-12-23
AU2019243242A1 (en) 2020-03-12
WO2019190192A1 (ko) 2019-10-03
CA3073180C (en) 2024-03-12
BR112020003867A2 (pt) 2020-10-27
US20200248217A1 (en) 2020-08-06
KR102028554B1 (ko) 2019-10-04
JP2021517802A (ja) 2021-07-29
TW202117002A (zh) 2021-05-01
EP4130281A1 (en) 2023-02-08
CA3073180A1 (en) 2019-10-03
CA3073177A1 (en) 2019-10-03
JP7089582B2 (ja) 2022-06-22
BR112020003467B8 (pt) 2024-01-16
CN111433366B (zh) 2024-01-09
BR112020003467B1 (pt) 2023-08-15
BR112020003867B1 (pt) 2022-04-12
JP2021515537A (ja) 2021-06-24
KR102137806B1 (ko) 2020-07-27
AR115302A1 (es) 2020-12-23
CN111655860A (zh) 2020-09-11
JP2022105171A (ja) 2022-07-12
EP3778901A1 (en) 2021-02-17
WO2019190193A1 (ko) 2019-10-03
CN111433366A (zh) 2020-07-17
TW201945535A (zh) 2019-12-01
JP7125477B2 (ja) 2022-08-24
KR20190113649A (ko) 2019-10-08
JP7607613B2 (ja) 2024-12-27
US20210002655A1 (en) 2021-01-07
CN111655860B (zh) 2023-10-03
TWI716841B (zh) 2021-01-21
EP3778900A4 (en) 2021-12-22
MY202021A (en) 2024-03-29
EP3778901A4 (en) 2021-12-22
KR20200090693A (ko) 2020-07-29
AU2019243241B2 (en) 2022-12-01

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