JP7064439B2 - 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット - Google Patents

単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット Download PDF

Info

Publication number
JP7064439B2
JP7064439B2 JP2018543062A JP2018543062A JP7064439B2 JP 7064439 B2 JP7064439 B2 JP 7064439B2 JP 2018543062 A JP2018543062 A JP 2018543062A JP 2018543062 A JP2018543062 A JP 2018543062A JP 7064439 B2 JP7064439 B2 JP 7064439B2
Authority
JP
Japan
Prior art keywords
nucleic acid
barcode
sequence
barcodes
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018543062A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018538006A5 (cg-RX-API-DMAC7.html
JP2018538006A (ja
Inventor
ギャリー ウィジー
Original Assignee
アトレカ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アトレカ インコーポレイテッド filed Critical アトレカ インコーポレイテッド
Publication of JP2018538006A publication Critical patent/JP2018538006A/ja
Publication of JP2018538006A5 publication Critical patent/JP2018538006A5/ja
Application granted granted Critical
Publication of JP7064439B2 publication Critical patent/JP7064439B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/319—Photocleavage, photolysis, photoactivation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Toxicology (AREA)
JP2018543062A 2015-11-04 2016-11-04 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット Active JP7064439B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562250900P 2015-11-04 2015-11-04
US62/250,900 2015-11-04
PCT/US2016/060576 WO2017079593A1 (en) 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells

Publications (3)

Publication Number Publication Date
JP2018538006A JP2018538006A (ja) 2018-12-27
JP2018538006A5 JP2018538006A5 (cg-RX-API-DMAC7.html) 2019-12-12
JP7064439B2 true JP7064439B2 (ja) 2022-05-10

Family

ID=57396822

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018543062A Active JP7064439B2 (ja) 2015-11-04 2016-11-04 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット

Country Status (9)

Country Link
US (2) US11098304B2 (cg-RX-API-DMAC7.html)
EP (1) EP3371309B1 (cg-RX-API-DMAC7.html)
JP (1) JP7064439B2 (cg-RX-API-DMAC7.html)
KR (1) KR20180097536A (cg-RX-API-DMAC7.html)
CN (1) CN108473984A (cg-RX-API-DMAC7.html)
AU (1) AU2016348439B2 (cg-RX-API-DMAC7.html)
CA (1) CA3004310A1 (cg-RX-API-DMAC7.html)
SG (1) SG11201803646YA (cg-RX-API-DMAC7.html)
WO (1) WO2017079593A1 (cg-RX-API-DMAC7.html)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
EP2920725B1 (en) 2012-11-19 2021-11-10 Apton Biosystems, Inc. Digital analysis of molecular analytes using single molecule detection
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
JP6659575B2 (ja) 2014-04-21 2020-03-04 ナテラ， インコーポレイテッド 変異の検出および染色体分節の倍数性
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
IL286474B2 (en) 2014-06-23 2023-11-01 Massachusetts Gen Hospital Genome-wide random identification of DSBS assessed by sequencing (guide-sequence)
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
AU2016319110B2 (en) 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
KR102395450B1 (ko) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. 핵산 라이브러리 정규화를 위한 방법 및 조성물
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) * 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CA3056765C (en) 2017-03-17 2024-04-02 Apton Biosystems, Inc. Sequencing and high resolution imaging
US11530436B2 (en) * 2017-05-23 2022-12-20 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) * 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
JP7581046B2 (ja) 2017-08-23 2024-11-12 ザ ジェネラル ホスピタル コーポレイション 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ
US20200217850A1 (en) * 2017-09-15 2020-07-09 Apton Biosystems, Inc. Heterogeneous single cell profiling using molecular barcoding
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
US11891600B2 (en) * 2017-11-06 2024-02-06 Illumina, Inc. Nucleic acid indexing techniques
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) * 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN111492068B (zh) * 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019200341A1 (en) * 2018-04-13 2019-10-17 The Board Of Trustees Of The Leland Stanford Junior University Nucleic acid quantification using concentration-specific barcodes
AU2019251504B2 (en) 2018-04-14 2025-09-25 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor DNA
WO2019204378A1 (en) * 2018-04-17 2019-10-24 The General Hospital Corporation Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CN118910215A (zh) 2018-05-03 2024-11-08 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
AU2019301750A1 (en) 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
WO2020037213A1 (en) 2018-08-17 2020-02-20 Genentech, Inc. In vitro transcytosis assay
CN112912513A (zh) * 2018-08-28 2021-06-04 贝克顿迪金森公司 使用糖类结合试剂和膜透过性试剂进行样品多重化
KR20210047364A (ko) 2018-09-19 2021-04-29 앱톤 바이오시스템즈, 인코포레이티드 밀집 패킹된 분석물층 및 검출 방법
CN112739824A (zh) * 2018-09-24 2021-04-30 百时美施贵宝公司 条形码化肽-mhc复合物及其用途
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
CN113015740A (zh) 2018-10-05 2021-06-22 德克萨斯大学系统董事会 固相n-末端肽的捕获和释放
CN113195085B (zh) 2018-10-11 2024-04-19 伯克利之光生命科技公司 用于识别优化蛋白产生的系统和方法以及其试剂盒
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
MX2021007777A (es) 2019-01-07 2021-10-13 Pivot Bio Inc Ensayos de colonizacion de plantas mediante el uso de codigos de barras microbianos naturales.
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020167656A1 (en) * 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP3980536A4 (en) * 2019-06-07 2023-11-15 Bio-Rad Laboratories, Inc. DISSOLUTION OF SEVERAL BEADS PER DROP
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
WO2021128036A1 (zh) * 2019-12-25 2021-07-01 苏州绘真生物科技有限公司 用于构建人单细胞tcr测序文库的试剂盒及其应用
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
CN115698284A (zh) * 2020-01-13 2023-02-03 福路伦特生物科学公司 模板乳化期间的逆转录
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
EP4106769A4 (en) * 2020-02-17 2024-03-27 Universal Sequencing Technology Corporation METHOD FOR BARCODING NUCLEIC ACIDS FOR DETECTION AND SEQUENCING
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
EP4159873A4 (en) * 2020-05-29 2024-07-17 Riken Method for treating cell population and method for analyzing genes included in cell population
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022221853A1 (en) * 2021-04-13 2022-10-20 Elegen Corp. Methods and compositions for cell-free cloning
WO2023048300A1 (ja) * 2021-09-27 2023-03-30 国立大学法人東京大学 細胞標識分子及び細胞の分析方法
GB202204903D0 (en) * 2022-04-04 2022-05-18 Univ Oxford Innovation Ltd chimeric artefact detectioin method
GB202211182D0 (en) * 2022-08-01 2022-09-14 Univ Oxford Innovation Ltd Bead-hashing
US20240052419A1 (en) * 2022-08-10 2024-02-15 Guardant Health, Inc. Methods and systems for detecting genetic variants
WO2025166174A1 (en) * 2024-02-01 2025-08-07 Bio-Rad Laboratories, Inc. Methods and compositions for bead deconvolution

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015103339A1 (en) 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
WO2016138490A1 (en) 2015-02-27 2016-09-01 Fluidigm Corporation Single-cell nucleic acids for high-throughput studies

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643722A (en) 1994-05-11 1997-07-01 Trustees Of Boston University Methods for the detection and isolation of proteins
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
EP1510251B1 (en) 2001-02-23 2006-09-06 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
JP4777238B2 (ja) 2004-03-23 2011-09-21 独立行政法人科学技術振興機構 微小液滴の生成方法及び装置
US20070031857A1 (en) * 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
CA2691364C (en) * 2007-06-19 2020-06-16 Stratos Genomics, Inc. High throughput nucleic acid sequencing by expansion
WO2009154303A2 (en) 2008-06-18 2009-12-23 Riken A method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence
EP3002337B1 (en) 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
EP2789694A1 (en) * 2009-04-02 2014-10-15 Fluidigm Corporation Microfluidic device with reaction product recovery system
CN101864477A (zh) * 2009-04-15 2010-10-20 上海聚类生物科技有限公司 一种利用高通量测序单通道复用技术进行microRNA表达谱测定的方法
EP2580351B1 (en) * 2010-06-09 2018-08-29 Keygene N.V. Combinatorial sequence barcodes for high throughput screening
ES2966040T3 (es) 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
DK2652155T3 (en) * 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
SI2702146T1 (sl) * 2011-04-28 2019-06-28 The Board Of Trustees Of The Leland Stanford Junior University Identifikacija polinukleotidov, povezanih z vzorcem
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
CN104080462B (zh) * 2011-12-16 2019-08-09 塔尔盖特基因生物技术有限公司 用于修饰预定的靶核酸序列的组合物和方法
CN104220602B (zh) * 2012-02-01 2018-10-30 合成基因组股份有限公司 用于合成错误最小化核酸分子的材料和方法
AU2013246050B2 (en) * 2012-04-13 2017-03-16 Adaptive Biotechnologies Corp. Detection and quantitation of sample contamination in immune repertoire analysis
SG11201500313YA (en) * 2012-07-24 2015-02-27 Sequenta Inc Single cell analysis using sequence tags
JP6338221B2 (ja) 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
JP6454281B2 (ja) * 2012-11-05 2019-01-16 タカラ バイオ ユーエスエー， インコーポレイテッド バーコード化する核酸
CA2906218A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
BR112015032512A8 (pt) * 2013-06-27 2020-01-07 10X Genomics Inc método de implementação de código de barras de materiais de amostra
US9708657B2 (en) * 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN105579587A (zh) 2013-08-23 2016-05-11 惠氏公司 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
EP3039158B1 (en) * 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
CA2923812C (en) 2013-10-17 2023-10-17 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
CN107075543B (zh) * 2014-04-21 2021-11-16 哈佛学院院长及董事 用于条形码化核酸的系统和方法
EA035092B1 (ru) 2014-05-14 2020-04-27 Барбара Бурвинкель Синтез двухцепочечных нуклеиновых кислот
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
SG10201911069WA (en) * 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016172373A1 (en) * 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015103339A1 (en) 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
WO2016138490A1 (en) 2015-02-27 2016-09-01 Fluidigm Corporation Single-cell nucleic acids for high-throughput studies

Also Published As

Publication number Publication date
SG11201803646YA (en) 2018-05-30
AU2016348439B2 (en) 2023-03-09
AU2016348439A1 (en) 2018-05-10
US20180320171A1 (en) 2018-11-08
EP3371309B1 (en) 2023-07-05
US11098304B2 (en) 2021-08-24
JP2018538006A (ja) 2018-12-27
WO2017079593A1 (en) 2017-05-11
US20210380974A1 (en) 2021-12-09
CN108473984A (zh) 2018-08-31
EP3371309A1 (en) 2018-09-12
KR20180097536A (ko) 2018-08-31
CA3004310A1 (en) 2017-05-11

Similar Documents

Publication Publication Date Title
JP7064439B2 (ja) 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
US11713457B2 (en) Methods and systems for processing polynucleotides
US10584381B2 (en) Methods and systems for processing polynucleotides
US10337061B2 (en) Methods and systems for processing polynucleotides
US10273541B2 (en) Methods and systems for processing polynucleotides
WO2019032760A1 (en) IMPROVED METHOD OF ANALYZING NUCLEIC ACID CONTENT FROM MULTIPLE BIOLOGICAL PARTICLES
US20220325275A1 (en) Methods of Barcoding Nucleic Acid for Detection and Sequencing
US20150133310A1 (en) Nucleic acid sequencing systems and methods
EP4569102A1 (en) Barcoding of nucleic acids from single cells
US12312640B2 (en) Analysis of nucleic acid sequences
Alam et al. Microfluidics in Genomics
CN114568027A (zh) 用于确定细胞轨迹的单细胞分析的方法和设备

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191101

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20191101

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200923

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20201221

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20210120

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210318

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210729

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20211027

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220121

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220228

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220330

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220422

R150 Certificate of patent or registration of utility model

Ref document number: 7064439

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150