JP7033591B2 - 治療用オリゴヌクレオチドの捕捉および検出 - Google Patents

治療用オリゴヌクレオチドの捕捉および検出 Download PDF

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JP7033591B2
JP7033591B2 JP2019524385A JP2019524385A JP7033591B2 JP 7033591 B2 JP7033591 B2 JP 7033591B2 JP 2019524385 A JP2019524385 A JP 2019524385A JP 2019524385 A JP2019524385 A JP 2019524385A JP 7033591 B2 JP7033591 B2 JP 7033591B2
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nucleoside
oligonucleotide
modified
capture probe
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JP2019534025A (ja
JP2019534025A5 (enExample
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マス オーボー イェンスン
ラース ヨーンソン
ルカス キールピンスキ
モーデン リンドウ
ヨーナス ヴィケソー
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ロシュ イノベーション センター コペンハーゲン エーエス
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
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    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step

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  • Chemical & Material Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2019524385A 2016-11-11 2017-11-09 治療用オリゴヌクレオチドの捕捉および検出 Expired - Fee Related JP7033591B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16198340 2016-11-11
EP16198340.8 2016-11-11
PCT/EP2017/078695 WO2018087200A1 (en) 2016-11-11 2017-11-09 Therapeutic oligonucleotides capture and detection

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JP2019534025A JP2019534025A (ja) 2019-11-28
JP2019534025A5 JP2019534025A5 (enExample) 2020-01-16
JP7033591B2 true JP7033591B2 (ja) 2022-03-10

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US (1) US20190284621A1 (enExample)
EP (1) EP3538671A1 (enExample)
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RU2019101298A (ru) 2016-07-01 2020-08-03 Ф. Хоффманн-Ля Рош Аг Антисмысловые олигонуклеотиды для модулирования экспрессии htra1
EP3568481A1 (en) * 2017-01-13 2019-11-20 Roche Innovation Center Copenhagen A/S Antisense oligonucleotides for modulating relb expression
US20190055564A1 (en) * 2017-06-01 2019-02-21 F. Hoffmann-La Roche Ag Antisense oligonucleotides for modulating htra1 expression
WO2021095829A1 (ja) * 2019-11-14 2021-05-20 積水メディカル株式会社 オリゴヌクレオチドの検出又は定量方法
WO2021144587A1 (en) * 2020-01-16 2021-07-22 Dnae Diagnostics Limited Compositions, kits and methods for isolating target polynucleotides
WO2022099216A1 (en) * 2020-11-09 2022-05-12 Children's Medical Center Corporation Systems and methods for high throughput screening of molecular interactions
GB202217984D0 (en) * 2022-11-30 2023-01-11 Qbiotix Ltd Assays

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003533971A (ja) 1999-11-12 2003-11-18 アイシス・ファーマシューティカルス・インコーポレーテッド オリゴヌクレオチドを定量するための方法
JP2008545430A (ja) 2005-05-31 2008-12-18 アプレラ コーポレイション 短い核酸のマルチプレックス増幅
JP2009509565A (ja) 2005-10-03 2009-03-12 アプレラ コーポレイション 核酸を増幅するための組成物、方法およびキット
WO2013153911A1 (ja) 2012-04-12 2013-10-17 国立大学法人東京大学 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法
WO2014110272A1 (en) 2013-01-09 2014-07-17 The Penn State Research Foundation Low sequence bias single-stranded dna ligation
JP2015511819A (ja) 2012-02-27 2015-04-23 セルラー リサーチ， インコーポレイテッド 分子計数のための組成物およびキット

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
KR100414936B1 (ko) 1997-09-12 2004-01-13 엑시콘 에이/에스 이환 및 삼환 뉴클레오시드, 뉴클레오타이드 및올리고뉴클레오타이드 동족체
NZ513402A (en) 1999-02-12 2003-06-30 Sankyo Co Novel nucleosides and oligonucleotide analogues
PT1178999E (pt) 1999-05-04 2007-06-26 Santaris Pharma As Análogos de l-ribo-lna
DK2752488T3 (da) 2002-11-18 2020-04-20 Roche Innovation Ct Copenhagen As Antisense-design
WO2007025281A2 (en) * 2005-08-24 2007-03-01 Applera Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
ES2516815T3 (es) 2006-01-27 2014-10-31 Isis Pharmaceuticals, Inc. Análogos de ácidos nucleicos bicíclicos modificados en la posición 6
CA2651453C (en) 2006-05-11 2014-10-14 Isis Pharmaceuticals, Inc. 5'-modified bicyclic nucleic acid analogs
US7666854B2 (en) 2006-05-11 2010-02-23 Isis Pharmaceuticals, Inc. Bis-modified bicyclic nucleic acid analogs
EP1914317A1 (en) 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length
WO2008150729A2 (en) 2007-05-30 2008-12-11 Isis Pharmaceuticals, Inc. N-substituted-aminomethylene bridged bicyclic nucleic acid analogs
EP2173760B2 (en) 2007-06-08 2015-11-04 Isis Pharmaceuticals, Inc. Carbocyclic bicyclic nucleic acid analogs
AU2008272918B2 (en) 2007-07-05 2012-09-13 Isis Pharmaceuticals, Inc. 6-disubstituted bicyclic nucleic acid analogs
US8546556B2 (en) 2007-11-21 2013-10-01 Isis Pharmaceuticals, Inc Carbocyclic alpha-L-bicyclic nucleic acid analogs
WO2010036698A1 (en) 2008-09-24 2010-04-01 Isis Pharmaceuticals, Inc. Substituted alpha-l-bicyclic nucleosides
US9012421B2 (en) 2009-08-06 2015-04-21 Isis Pharmaceuticals, Inc. Bicyclic cyclohexose nucleic acid analogs
US8846637B2 (en) 2010-06-08 2014-09-30 Isis Pharmaceuticals, Inc. Substituted 2′-amino and 2′-thio-bicyclic nucleosides and oligomeric compounds prepared therefrom
CN104136451A (zh) 2011-09-07 2014-11-05 玛瑞纳生物技术有限公司 具有构象限制的单体的核酸化合物的合成和用途
US9221864B2 (en) 2012-04-09 2015-12-29 Isis Pharmaceuticals, Inc. Tricyclic nucleic acid analogs

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003533971A (ja) 1999-11-12 2003-11-18 アイシス・ファーマシューティカルス・インコーポレーテッド オリゴヌクレオチドを定量するための方法
JP2008545430A (ja) 2005-05-31 2008-12-18 アプレラ コーポレイション 短い核酸のマルチプレックス増幅
JP2009509565A (ja) 2005-10-03 2009-03-12 アプレラ コーポレイション 核酸を増幅するための組成物、方法およびキット
JP2015511819A (ja) 2012-02-27 2015-04-23 セルラー リサーチ， インコーポレイテッド 分子計数のための組成物およびキット
WO2013153911A1 (ja) 2012-04-12 2013-10-17 国立大学法人東京大学 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法
WO2014110272A1 (en) 2013-01-09 2014-07-17 The Penn State Research Foundation Low sequence bias single-stranded dna ligation

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JP2019534025A (ja) 2019-11-28
US20190284621A1 (en) 2019-09-19
EP3538671A1 (en) 2019-09-18

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