JP7007367B2 - 微生物の存在を判定し、前記微生物を同定するための方法、システム、及びコンピュータプログラム製品 - Google Patents

微生物の存在を判定し、前記微生物を同定するための方法、システム、及びコンピュータプログラム製品 Download PDF

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JP7007367B2
JP7007367B2 JP2019510638A JP2019510638A JP7007367B2 JP 7007367 B2 JP7007367 B2 JP 7007367B2 JP 2019510638 A JP2019510638 A JP 2019510638A JP 2019510638 A JP2019510638 A JP 2019510638A JP 7007367 B2 JP7007367 B2 JP 7007367B2
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image
petri dish
processed
processed image
colonies
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JP2019531065A5 (enExample
JP2019531065A (ja
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ギヨーム スートゥラ，
フィリピーヌ バルラス，
デルフィーヌ アルシェニー，
ジェラルディーヌ デュラン，
コリーヌ フルシロン，
ジャン－フランソワ ゴルセ，
ギヨーム ペラン，
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Biomerieux SA
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Biomerieux SA
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/20—Image enhancement or restoration using local operators
    • G06T5/30—Erosion or dilatation, e.g. thinning
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    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
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    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
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    • G06T7/0016—Biomedical image inspection using an image reference approach involving temporal comparison
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    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/162—Segmentation; Edge detection involving graph-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
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    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20036—Morphological image processing
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    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
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    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2019510638A 2016-09-02 2017-08-30 微生物の存在を判定し、前記微生物を同定するための方法、システム、及びコンピュータプログラム製品 Expired - Fee Related JP7007367B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP16187153.8A EP3290524A1 (en) 2016-09-02 2016-09-02 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms
EP16187153.8 2016-09-02
EP16306113.8 2016-09-05
EP16306113 2016-09-05
PCT/EP2017/071775 WO2018041900A1 (en) 2016-09-02 2017-08-30 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms

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JP2019531065A JP2019531065A (ja) 2019-10-31
JP2019531065A5 JP2019531065A5 (enExample) 2020-10-08
JP7007367B2 true JP7007367B2 (ja) 2022-01-24

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US (1) US10968473B2 (enExample)
EP (1) EP3507378B1 (enExample)
JP (1) JP7007367B2 (enExample)
CN (1) CN109661473B (enExample)
ES (1) ES2823151T3 (enExample)
WO (1) WO2018041900A1 (enExample)

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CN111286523A (zh) * 2020-02-20 2020-06-16 中国水产科学研究院黄海水产研究所 一种细菌的三原色鉴别及计数方法
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
CN116263955A (zh) * 2023-01-18 2023-06-16 浙江泰林医学工程有限公司 一种自动菌落计数方法及系统
CN116128845B (zh) * 2023-02-10 2024-05-07 甘肃省科学院生物研究所 一种基于随机漫步的菌落自动计数方法及菌落计数器
CN117557583B (zh) * 2024-01-10 2024-03-15 江苏锡澄环境科学研究院有限公司 基于图像处理的电泳线废水处理效果视觉检测方法
CN117953495B (zh) * 2024-03-27 2024-06-04 北京大学口腔医学院 一种口腔微生物菌群群落分割方法
CN118196087B (zh) * 2024-05-15 2024-08-02 江苏海枫达生物科技有限公司 一种基于图像采集的微生物菌落检测系统及其方法
CN119842475A (zh) * 2025-03-18 2025-04-18 浙江鹰旸医药研发有限公司 一种微生物智能培养识别分析系统及方法

Citations (5)

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JP2002125696A (ja) 2000-10-19 2002-05-08 National Institute Of Advanced Industrial & Technology 媒体中の生細胞の検出および該媒体のｐＨ測定のための方法
JP2003116593A (ja) 2001-10-17 2003-04-22 Hakuju Inst For Health Science Co Ltd 微生物の判定方法およびその装置
JP2014513960A (ja) 2011-05-06 2014-06-19 ビオメリュー バイオ画像化方法及びシステム
JP2015536140A (ja) 2012-11-07 2015-12-21 バイオメリュー バイオイメージング法
JP2016052256A (ja) 2014-09-02 2016-04-14 株式会社エヌテック 微生物検出方法、微生物検出装置及びプログラム

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US5694478A (en) * 1994-12-15 1997-12-02 Minnesota Mining And Manufacturing Company Method and apparatus for detecting and identifying microbial colonies
US20020086289A1 (en) * 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
CN1536090A (zh) * 2003-04-07 2004-10-13 中国人民解放军军事医学科学院卫生学 食源性致病菌快速检测基因芯片及其应用
US20040253660A1 (en) * 2003-06-12 2004-12-16 Giles Scientific, Inc. Automated microbiological testing apparatus and method
US20080153125A1 (en) * 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
CA2715566C (en) * 2008-02-19 2019-06-04 Becton, Dickinson And Company Systems and methods for presumptive identification of microorganism type in a culture
JP5439096B2 (ja) 2009-09-08 2014-03-12 株式会社日立ハイテクノロジーズ 細菌分類装置および細菌検査前処理装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002125696A (ja) 2000-10-19 2002-05-08 National Institute Of Advanced Industrial & Technology 媒体中の生細胞の検出および該媒体のｐＨ測定のための方法
JP2003116593A (ja) 2001-10-17 2003-04-22 Hakuju Inst For Health Science Co Ltd 微生物の判定方法およびその装置
JP2014513960A (ja) 2011-05-06 2014-06-19 ビオメリュー バイオ画像化方法及びシステム
JP2015536140A (ja) 2012-11-07 2015-12-21 バイオメリュー バイオイメージング法
JP2016052256A (ja) 2014-09-02 2016-04-14 株式会社エヌテック 微生物検出方法、微生物検出装置及びプログラム

Also Published As

Publication number Publication date
EP3507378A1 (en) 2019-07-10
WO2018041900A1 (en) 2018-03-08
CN109661473A (zh) 2019-04-19
ES2823151T3 (es) 2021-05-06
JP2019531065A (ja) 2019-10-31
EP3507378B1 (en) 2020-07-29
US20190203257A1 (en) 2019-07-04
US10968473B2 (en) 2021-04-06
CN109661473B (zh) 2022-06-07

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