ES2823151T3 - Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos - Google Patents

Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos Download PDF

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Publication number
ES2823151T3
ES2823151T3 ES17758553T ES17758553T ES2823151T3 ES 2823151 T3 ES2823151 T3 ES 2823151T3 ES 17758553 T ES17758553 T ES 17758553T ES 17758553 T ES17758553 T ES 17758553T ES 2823151 T3 ES2823151 T3 ES 2823151T3
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Prior art keywords
petri dish
image
processed
colonies
pixels
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ES17758553T
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English (en)
Spanish (es)
Inventor
Guillaume Sutra
Philippine Barlas
Delphine Archeny
Géraldine Durand
Corine Fulchiron
Jean-François Gorse
Guillaume Perrin
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Biomerieux SA
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Biomerieux SA
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Priority claimed from EP16187153.8A external-priority patent/EP3290524A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/20—Image enhancement or restoration using local operators
    • G06T5/30—Erosion or dilatation, e.g. thinning
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G06T7/0014—Biomedical image inspection using an image reference approach
    • G06T7/0016—Biomedical image inspection using an image reference approach involving temporal comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/162—Segmentation; Edge detection involving graph-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20036—Morphological image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Biomedical Technology (AREA)
  • Multimedia (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
ES17758553T 2016-09-02 2017-08-30 Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos Active ES2823151T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16187153.8A EP3290524A1 (en) 2016-09-02 2016-09-02 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms
EP16306113 2016-09-05
PCT/EP2017/071775 WO2018041900A1 (en) 2016-09-02 2017-08-30 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms

Publications (1)

Publication Number Publication Date
ES2823151T3 true ES2823151T3 (es) 2021-05-06

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ES17758553T Active ES2823151T3 (es) 2016-09-02 2017-08-30 Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos

Country Status (6)

Country Link
US (1) US10968473B2 (enExample)
EP (1) EP3507378B1 (enExample)
JP (1) JP7007367B2 (enExample)
CN (1) CN109661473B (enExample)
ES (1) ES2823151T3 (enExample)
WO (1) WO2018041900A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111286523A (zh) * 2020-02-20 2020-06-16 中国水产科学研究院黄海水产研究所 一种细菌的三原色鉴别及计数方法
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
CN116263955A (zh) * 2023-01-18 2023-06-16 浙江泰林医学工程有限公司 一种自动菌落计数方法及系统
CN116128845B (zh) * 2023-02-10 2024-05-07 甘肃省科学院生物研究所 一种基于随机漫步的菌落自动计数方法及菌落计数器
CN117557583B (zh) * 2024-01-10 2024-03-15 江苏锡澄环境科学研究院有限公司 基于图像处理的电泳线废水处理效果视觉检测方法
CN117953495B (zh) * 2024-03-27 2024-06-04 北京大学口腔医学院 一种口腔微生物菌群群落分割方法
CN118196087B (zh) * 2024-05-15 2024-08-02 江苏海枫达生物科技有限公司 一种基于图像采集的微生物菌落检测系统及其方法
CN119842475A (zh) * 2025-03-18 2025-04-18 浙江鹰旸医药研发有限公司 一种微生物智能培养识别分析系统及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5694478A (en) * 1994-12-15 1997-12-02 Minnesota Mining And Manufacturing Company Method and apparatus for detecting and identifying microbial colonies
US20020086289A1 (en) * 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
JP2002125696A (ja) 2000-10-19 2002-05-08 National Institute Of Advanced Industrial & Technology 媒体中の生細胞の検出および該媒体のｐＨ測定のための方法
JP2003116593A (ja) 2001-10-17 2003-04-22 Hakuju Inst For Health Science Co Ltd 微生物の判定方法およびその装置
CN1536090A (zh) * 2003-04-07 2004-10-13 中国人民解放军军事医学科学院卫生学 食源性致病菌快速检测基因芯片及其应用
US20040253660A1 (en) * 2003-06-12 2004-12-16 Giles Scientific, Inc. Automated microbiological testing apparatus and method
US20080153125A1 (en) * 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
CA2715566C (en) * 2008-02-19 2019-06-04 Becton, Dickinson And Company Systems and methods for presumptive identification of microorganism type in a culture
JP5439096B2 (ja) 2009-09-08 2014-03-12 株式会社日立ハイテクノロジーズ 細菌分類装置および細菌検査前処理装置
EP2520923A1 (en) * 2011-05-06 2012-11-07 bioMérieux Bio-imaging method and system
EP2731051A1 (en) * 2012-11-07 2014-05-14 bioMérieux Bio-imaging method
JP6223932B2 (ja) 2014-09-02 2017-11-01 株式会社エヌテック 微生物検出方法、微生物検出装置及びプログラム

Also Published As

Publication number Publication date
EP3507378A1 (en) 2019-07-10
WO2018041900A1 (en) 2018-03-08
CN109661473A (zh) 2019-04-19
JP2019531065A (ja) 2019-10-31
EP3507378B1 (en) 2020-07-29
US20190203257A1 (en) 2019-07-04
US10968473B2 (en) 2021-04-06
JP7007367B2 (ja) 2022-01-24
CN109661473B (zh) 2022-06-07

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