JP6952706B2 - エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 - Google Patents

エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Download PDF

Info

Publication number
JP6952706B2
JP6952706B2 JP2018546669A JP2018546669A JP6952706B2 JP 6952706 B2 JP6952706 B2 JP 6952706B2 JP 2018546669 A JP2018546669 A JP 2018546669A JP 2018546669 A JP2018546669 A JP 2018546669A JP 6952706 B2 JP6952706 B2 JP 6952706B2
Authority
JP
Japan
Prior art keywords
antibody
epitope
epitope tag
seq
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018546669A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019512224A5 (enExample
JP2019512224A (ja
Inventor
マイケル ファレル，
マイケル ファレル，
エリック メイ，
エリック メイ，
レイ タン，
レイ タン，
ウェンチュン チャン，
ウェンチュン チャン，
アントニー ハバード，
アントニー ハバード，
ツー−シュエン ツァオ，
ツー−シュエン ツァオ，
Original Assignee
ヴェンタナ メディカル システムズ， インク．
ヴェンタナ メディカル システムズ， インク．
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ヴェンタナ メディカル システムズ， インク．, ヴェンタナ メディカル システムズ， インク． filed Critical ヴェンタナ メディカル システムズ， インク．
Publication of JP2019512224A publication Critical patent/JP2019512224A/ja
Publication of JP2019512224A5 publication Critical patent/JP2019512224A5/ja
Priority to JP2021156935A priority Critical patent/JP7174126B2/ja
Application granted granted Critical
Publication of JP6952706B2 publication Critical patent/JP6952706B2/ja
Priority to JP2022177332A priority patent/JP2023024990A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546669A 2016-03-08 2017-03-07 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Active JP6952706B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021156935A JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021156935A Division JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Publications (3)

Publication Number Publication Date
JP2019512224A JP2019512224A (ja) 2019-05-16
JP2019512224A5 JP2019512224A5 (enExample) 2020-04-16
JP6952706B2 true JP6952706B2 (ja) 2021-10-20

Family

ID=58361148

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018546669A Active JP6952706B2 (ja) 2016-03-08 2017-03-07 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2021156935A Active JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2021156935A Active JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Country Status (6)

Country Link
US (3) US11112413B2 (enExample)
EP (1) EP3426684B1 (enExample)
JP (3) JP6952706B2 (enExample)
AU (1) AU2017229370B2 (enExample)
CA (1) CA3016555A1 (enExample)
WO (1) WO2017155996A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3002674A1 (en) 2015-10-30 2017-05-04 Aleta Biotherapeutics Inc. Compositions and methods for treatment of cancer
MX2018005348A (es) 2015-10-30 2018-08-14 Aleta Biotherapeutics Inc Composiciones y metodos para transduccion de tumores.
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
WO2019094095A1 (en) * 2017-11-07 2019-05-16 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
CN113710365B (zh) * 2019-04-03 2023-12-19 万迈医疗仪器有限公司 用于在免疫测定中重复使用半抗原涂覆的探针的方法
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
WO2023162042A1 (ja) 2022-02-22 2023-08-31 日本電信電話株式会社 センサー付きハイドロゲル流路型デバイス
JP2025532064A (ja) 2022-09-22 2025-09-29 アジレント・テクノロジーズ・インク 免疫組織化学（ｉｈｃ）プロトコールにおける使用のためおよびがんを診断するための抗ヒトｉｃｏｓ抗体

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1564604A (en) 1925-06-12 1925-12-08 Chas S Hodges Water wheel
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
AU763354B2 (en) 1998-02-27 2003-07-17 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US7632652B2 (en) 2003-06-24 2009-12-15 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced in situ detection of immunohistochemical epitopes and nucleic acid sequences
AU2006239315B2 (en) 2005-04-28 2012-03-01 Ventana Medical Systems, Inc. Enzymes conjugated to antiobodies via a PEG heterobifuctional linker
EP3095467B1 (en) 2005-11-23 2020-05-06 Ventana Medical Systems, Inc. Antibody-enzyme conjugate
DK2078197T3 (en) 2006-11-01 2016-05-23 Ventana Med Syst Inc Haptenes, hapten conjugates, compositions thereof and processes for their preparation and use
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
US20120034223A1 (en) * 2008-11-06 2012-02-09 Hall Christopher J Methods of improving the therapeutic efficacy and utility of antibody fragments
EP2993478B1 (en) 2009-10-19 2022-08-24 Ventana Medical Systems, Inc. Device and method for slide caching
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
AU2011274369A1 (en) 2010-07-02 2012-12-06 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CA3062786C (en) * 2010-07-09 2022-04-19 Janssen Vaccines & Prevention B.V. Anti-human respiratory syncytial virus (rsv) antibodies and methods of use
CA2940439C (en) 2014-02-24 2024-03-19 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
EP4215898A1 (en) 2015-04-20 2023-07-26 Ventana Medical Systems, Inc. Inkjet deposition of reagents for histological samples
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Also Published As

Publication number Publication date
CA3016555A1 (en) 2017-09-14
US20220057408A1 (en) 2022-02-24
AU2017229370B2 (en) 2021-03-11
AU2017229370A1 (en) 2018-08-16
WO2017155996A1 (en) 2017-09-14
US11112413B2 (en) 2021-09-07
JP7174126B2 (ja) 2022-11-17
US11988673B2 (en) 2024-05-21
US20240230667A1 (en) 2024-07-11
JP2023024990A (ja) 2023-02-21
EP3426684A1 (en) 2019-01-16
US20190094242A1 (en) 2019-03-28
JP2019512224A (ja) 2019-05-16
JP2022003064A (ja) 2022-01-11
EP3426684B1 (en) 2024-10-09

Similar Documents

Publication Publication Date Title
JP7174126B2 (ja) エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
US12126880B2 (en) Digitally enhanced microscopy for multiplexed histology
JP6832930B2 (ja) Ｐｄ−ｌ１陽性腫瘍組織における免疫細胞を同定する方法
US20190018018A1 (en) Signaling conjugates and methods of use
WO2018055014A1 (en) Methods and systems for scoring extracellular matrix biomarkers in tumor samples
US20230151361A1 (en) Peptide nucleic acid conjugates
WO2024137817A1 (en) Materials and methods for evaluation of antigen presentation machinery components and uses thereof
JP2025532064A (ja) 免疫組織化学（ｉｈｃ）プロトコールにおける使用のためおよびがんを診断するための抗ヒトｉｃｏｓ抗体

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200306

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200306

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210316

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210527

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210907

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210928

R150 Certificate of patent or registration of utility model

Ref document number: 6952706

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250