JP6952706B2 - エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 - Google Patents

エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Download PDF

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JP6952706B2
JP6952706B2 JP2018546669A JP2018546669A JP6952706B2 JP 6952706 B2 JP6952706 B2 JP 6952706B2 JP 2018546669 A JP2018546669 A JP 2018546669A JP 2018546669 A JP2018546669 A JP 2018546669A JP 6952706 B2 JP6952706 B2 JP 6952706B2
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antibody
epitope
epitope tag
seq
tag
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JP2019512224A5 (enExample
JP2019512224A (ja
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マイケル ファレル，
マイケル ファレル，
エリック メイ，
エリック メイ，
レイ タン，
レイ タン，
ウェンチュン チャン，
ウェンチュン チャン，
アントニー ハバード，
アントニー ハバード，
ツー−シュエン ツァオ，
ツー−シュエン ツァオ，
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ヴェンタナ メディカル システムズ， インク．
ヴェンタナ メディカル システムズ， インク．
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Publication of JP2019512224A publication Critical patent/JP2019512224A/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546669A 2016-03-08 2017-03-07 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Active JP6952706B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021156935A JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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JP2021156935A Division JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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JP2019512224A JP2019512224A (ja) 2019-05-16
JP2019512224A5 JP2019512224A5 (enExample) 2020-04-16
JP6952706B2 true JP6952706B2 (ja) 2021-10-20

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JP2021156935A Active JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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US (3) US11112413B2 (enExample)
EP (1) EP3426684B1 (enExample)
JP (3) JP6952706B2 (enExample)
AU (1) AU2017229370B2 (enExample)
CA (1) CA3016555A1 (enExample)
WO (1) WO2017155996A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017075537A1 (en) 2015-10-30 2017-05-04 Aleta Biotherapeutics Inc. Compositions and methods for treatment of cancer
JP2018536434A (ja) 2015-10-30 2018-12-13 アレタ・バイオセラピューティクス・インコーポレイテッドＡｌｅｔａ Ｂｉｏｔｈｅｒａｐｅｕｔｉｃｓ Ｉｎｃ． 腫瘍形質導入のための組成物及び方法
EP3426684B1 (en) * 2016-03-08 2024-10-09 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
WO2019094095A1 (en) * 2017-11-07 2019-05-16 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
CN117696135A (zh) * 2019-04-03 2024-03-15 万迈医疗仪器有限公司 用于在免疫测定中重复使用半抗原涂覆的探针的方法
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
US20250153164A1 (en) 2022-02-22 2025-05-15 Nippon Telegraph And Telephone Corporation Hydrogel channel device with sensor
EP4590709A1 (en) 2022-09-22 2025-07-30 Agilent Technologies, Inc. Anti-human icos antibodies for use in immunohistochemistry (ihc) protocols and for diagnosing cancer

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1564604A (en) 1925-06-12 1925-12-08 Chas S Hodges Water wheel
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
EP1056541B1 (en) 1998-02-27 2017-10-25 Ventana Medical Systems, Inc. System and method of dispensing reagent
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
DE602004022165D1 (de) 2003-06-24 2009-09-03 Ventana Med Syst Inc Enzymkatalysierte metalldeposition zum verstärkten und nukleinsäuresequenzen
EP3144675A1 (en) 2005-04-28 2017-03-22 Ventana Medical Systems, Inc. Enzymes conjugated to antibodies via a peg heterobifuctional linker
CN101535244B (zh) 2005-11-23 2014-07-23 文塔納医疗系统公司 分子缀合物
ES2465465T3 (es) 2006-11-01 2014-06-05 Ventana Medical Systems, Inc. Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
EP2355849B1 (en) * 2008-11-06 2018-05-02 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments
SG10201403755RA (en) 2009-10-19 2014-10-30 Ventana Med Syst Inc Imaging System And Techniques
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
WO2012003476A2 (en) 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CN106188284B (zh) * 2010-07-09 2020-05-08 扬森疫苗与预防公司 抗人呼吸道合胞病毒(rsv)抗体以及使用方法
ES2713438T3 (es) 2014-02-24 2019-05-21 Ventana Med Syst Inc Amplificación de señal de análogos de quinona metidas
CA2980876C (en) 2015-04-20 2020-04-28 Ventana Medical Systems, Inc. Inkjet deposition of reagents for histological samples
EP3426684B1 (en) * 2016-03-08 2024-10-09 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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JP2023024990A (ja) 2023-02-21
US11112413B2 (en) 2021-09-07
EP3426684A1 (en) 2019-01-16
CA3016555A1 (en) 2017-09-14
US20220057408A1 (en) 2022-02-24
AU2017229370A1 (en) 2018-08-16
US20240230667A1 (en) 2024-07-11
JP2022003064A (ja) 2022-01-11
JP7174126B2 (ja) 2022-11-17
JP2019512224A (ja) 2019-05-16
US11988673B2 (en) 2024-05-21
AU2017229370B2 (en) 2021-03-11
WO2017155996A1 (en) 2017-09-14
EP3426684B1 (en) 2024-10-09
US20190094242A1 (en) 2019-03-28

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