AU2017229370B2 - Multiplexed immunohistochemistry using recombinant antibodies with epitope tags - Google Patents

Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Download PDF

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Publication number
AU2017229370B2
AU2017229370B2 AU2017229370A AU2017229370A AU2017229370B2 AU 2017229370 B2 AU2017229370 B2 AU 2017229370B2 AU 2017229370 A AU2017229370 A AU 2017229370A AU 2017229370 A AU2017229370 A AU 2017229370A AU 2017229370 B2 AU2017229370 B2 AU 2017229370B2
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Australia
Prior art keywords
epitope
antibody
tag
seq
tagged
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AU2017229370A
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AU2017229370A1 (en
Inventor
Michael Farrell
Antony HUBBARD
Eric May
Lei Tang
Tsu-Shuen Tsao
Wenjun Zhang
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2017229370A 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Active AU2017229370B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Publications (2)

Publication Number Publication Date
AU2017229370A1 AU2017229370A1 (en) 2018-08-16
AU2017229370B2 true AU2017229370B2 (en) 2021-03-11

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Family Applications (1)

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AU2017229370A Active AU2017229370B2 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Country Status (6)

Country Link
US (3) US11112413B2 (enExample)
EP (1) EP3426684B1 (enExample)
JP (3) JP6952706B2 (enExample)
AU (1) AU2017229370B2 (enExample)
CA (1) CA3016555A1 (enExample)
WO (1) WO2017155996A1 (enExample)

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CA3002674A1 (en) 2015-10-30 2017-05-04 Aleta Biotherapeutics Inc. Compositions and methods for treatment of cancer
MX2018005348A (es) 2015-10-30 2018-08-14 Aleta Biotherapeutics Inc Composiciones y metodos para transduccion de tumores.
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
WO2019094095A1 (en) * 2017-11-07 2019-05-16 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
CN113710365B (zh) * 2019-04-03 2023-12-19 万迈医疗仪器有限公司 用于在免疫测定中重复使用半抗原涂覆的探针的方法
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
WO2023162042A1 (ja) 2022-02-22 2023-08-31 日本電信電話株式会社 センサー付きハイドロゲル流路型デバイス
JP2025532064A (ja) 2022-09-22 2025-09-29 アジレント・テクノロジーズ・インク 免疫組織化学（ｉｈｃ）プロトコールにおける使用のためおよびがんを診断するための抗ヒトｉｃｏｓ抗体

Citations (2)

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WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
WO2010051635A1 (en) * 2008-11-06 2010-05-14 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments

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US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
AU763354B2 (en) 1998-02-27 2003-07-17 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US7632652B2 (en) 2003-06-24 2009-12-15 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced in situ detection of immunohistochemical epitopes and nucleic acid sequences
AU2006239315B2 (en) 2005-04-28 2012-03-01 Ventana Medical Systems, Inc. Enzymes conjugated to antiobodies via a PEG heterobifuctional linker
EP3095467B1 (en) 2005-11-23 2020-05-06 Ventana Medical Systems, Inc. Antibody-enzyme conjugate
DK2078197T3 (en) 2006-11-01 2016-05-23 Ventana Med Syst Inc Haptenes, hapten conjugates, compositions thereof and processes for their preparation and use
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
EP2993478B1 (en) 2009-10-19 2022-08-24 Ventana Medical Systems, Inc. Device and method for slide caching
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
AU2011274369A1 (en) 2010-07-02 2012-12-06 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CA3062786C (en) * 2010-07-09 2022-04-19 Janssen Vaccines & Prevention B.V. Anti-human respiratory syncytial virus (rsv) antibodies and methods of use
CA2940439C (en) 2014-02-24 2024-03-19 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
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AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
WO2010051635A1 (en) * 2008-11-06 2010-05-14 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments

Also Published As

Publication number Publication date
CA3016555A1 (en) 2017-09-14
US20220057408A1 (en) 2022-02-24
JP6952706B2 (ja) 2021-10-20
AU2017229370A1 (en) 2018-08-16
WO2017155996A1 (en) 2017-09-14
US11112413B2 (en) 2021-09-07
JP7174126B2 (ja) 2022-11-17
US11988673B2 (en) 2024-05-21
US20240230667A1 (en) 2024-07-11
JP2023024990A (ja) 2023-02-21
EP3426684A1 (en) 2019-01-16
US20190094242A1 (en) 2019-03-28
JP2019512224A (ja) 2019-05-16
JP2022003064A (ja) 2022-01-11
EP3426684B1 (en) 2024-10-09

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