AU2017229370B2 - Multiplexed immunohistochemistry using recombinant antibodies with epitope tags - Google Patents

Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Download PDF

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Publication number
AU2017229370B2
AU2017229370B2 AU2017229370A AU2017229370A AU2017229370B2 AU 2017229370 B2 AU2017229370 B2 AU 2017229370B2 AU 2017229370 A AU2017229370 A AU 2017229370A AU 2017229370 A AU2017229370 A AU 2017229370A AU 2017229370 B2 AU2017229370 B2 AU 2017229370B2
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Australia
Prior art keywords
epitope
antibody
tag
seq
tagged
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AU2017229370A
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AU2017229370A1 (en
Inventor
Michael Farrell
Antony HUBBARD
Eric May
Lei Tang
Tsu-Shuen Tsao
Wenjun Zhang
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2017229370A 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Active AU2017229370B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Publications (2)

Publication Number Publication Date
AU2017229370A1 AU2017229370A1 (en) 2018-08-16
AU2017229370B2 true AU2017229370B2 (en) 2021-03-11

Family

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Family Applications (1)

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AU2017229370A Active AU2017229370B2 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Country Status (6)

Country Link
US (3) US11112413B2 (enExample)
EP (1) EP3426684B1 (enExample)
JP (3) JP6952706B2 (enExample)
AU (1) AU2017229370B2 (enExample)
CA (1) CA3016555A1 (enExample)
WO (1) WO2017155996A1 (enExample)

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WO2017075537A1 (en) 2015-10-30 2017-05-04 Aleta Biotherapeutics Inc. Compositions and methods for treatment of cancer
JP2018536434A (ja) 2015-10-30 2018-12-13 アレタ・バイオセラピューティクス・インコーポレイテッドＡｌｅｔａ Ｂｉｏｔｈｅｒａｐｅｕｔｉｃｓ Ｉｎｃ． 腫瘍形質導入のための組成物及び方法
EP3426684B1 (en) * 2016-03-08 2024-10-09 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
WO2019094095A1 (en) * 2017-11-07 2019-05-16 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
CN117696135A (zh) * 2019-04-03 2024-03-15 万迈医疗仪器有限公司 用于在免疫测定中重复使用半抗原涂覆的探针的方法
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
US20250153164A1 (en) 2022-02-22 2025-05-15 Nippon Telegraph And Telephone Corporation Hydrogel channel device with sensor
EP4590709A1 (en) 2022-09-22 2025-07-30 Agilent Technologies, Inc. Anti-human icos antibodies for use in immunohistochemistry (ihc) protocols and for diagnosing cancer

Citations (2)

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WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
WO2010051635A1 (en) * 2008-11-06 2010-05-14 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments

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US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
EP1056541B1 (en) 1998-02-27 2017-10-25 Ventana Medical Systems, Inc. System and method of dispensing reagent
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
DE602004022165D1 (de) 2003-06-24 2009-09-03 Ventana Med Syst Inc Enzymkatalysierte metalldeposition zum verstärkten und nukleinsäuresequenzen
EP3144675A1 (en) 2005-04-28 2017-03-22 Ventana Medical Systems, Inc. Enzymes conjugated to antibodies via a peg heterobifuctional linker
CN101535244B (zh) 2005-11-23 2014-07-23 文塔納医疗系统公司 分子缀合物
ES2465465T3 (es) 2006-11-01 2014-06-05 Ventana Medical Systems, Inc. Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
SG10201403755RA (en) 2009-10-19 2014-10-30 Ventana Med Syst Inc Imaging System And Techniques
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
WO2012003476A2 (en) 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CN106188284B (zh) * 2010-07-09 2020-05-08 扬森疫苗与预防公司 抗人呼吸道合胞病毒(rsv)抗体以及使用方法
ES2713438T3 (es) 2014-02-24 2019-05-21 Ventana Med Syst Inc Amplificación de señal de análogos de quinona metidas
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EP3426684B1 (en) * 2016-03-08 2024-10-09 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
WO2010051635A1 (en) * 2008-11-06 2010-05-14 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments

Also Published As

Publication number Publication date
JP2023024990A (ja) 2023-02-21
US11112413B2 (en) 2021-09-07
EP3426684A1 (en) 2019-01-16
CA3016555A1 (en) 2017-09-14
JP6952706B2 (ja) 2021-10-20
US20220057408A1 (en) 2022-02-24
AU2017229370A1 (en) 2018-08-16
US20240230667A1 (en) 2024-07-11
JP2022003064A (ja) 2022-01-11
JP7174126B2 (ja) 2022-11-17
JP2019512224A (ja) 2019-05-16
US11988673B2 (en) 2024-05-21
WO2017155996A1 (en) 2017-09-14
EP3426684B1 (en) 2024-10-09
US20190094242A1 (en) 2019-03-28

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