JP6914326B2 - 折り紙ベースの試料分離装置 - Google Patents
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4005—Concentrating samples by transferring a selected component through a membrane
- G01N2001/4011—Concentrating samples by transferring a selected component through a membrane being a ion-exchange membrane
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Description
Claims (14)
- 複数のベースユニットを含むベース、
前記ベースの少なくとも一部領域に位置して試料の吸着を防止し、貯蔵空間または移動経路を区分するコーティング層、
前記コーティング層により領域が設定され、前記複数のベースユニットに位置し、前記試料から分離しようとする収集対象物または前記試料に含まれた前記収集対象物でない分離対象物を貯蔵するかまたは移動させる複数のリザーバ、
前記複数のリザーバのうち一部のリザーバと結合してイオンを選択的に透過させる選択的イオン透過層、及び
前記複数のベースユニットが折り畳まれて形成する前記収集対象物の移動経路の中間に位置して前記分離対象物を濾過する複数の濾過層、
を含む試料分離装置であって、
折り畳まれるように形成される前記選択的イオン透過膜に電界を印加すると、イオン濃度分極(ICP)が発生し、前記複数のベースユニットの中一部のベースユニットに枯渇領域と濃縮領域が明らかに区分されるように分離が起こり、
前記分離対象物の大きさより小さい異なる微細孔の大きさを有する前記濾過層は、前記収集対象物の移動経路の中間に位置することにより、前記収集対象物の移動経路である前記ベースユニットの個数を減らし、前記ベースユニットにおける前記収集対象物が濃縮される位置である濃縮領域が形成される位置を調整できることを特徴とする、試料分離装置。 - 前記ベースの一側末端に位置した第1末端ベースユニットは前記リザーバとして第1末端リザーバを含み、前記ベースの他側末端に位置した第2末端ベースユニットは前記リザーバとして第2末端リザーバを含み、前記第1末端リザーバと前記第2末端リザーバは各々前記選択的イオン透過層と連結されることを特徴とする、請求項1に記載の試料分離装置。
- 前記選択的イオン透過層は、前記第1末端ベースユニットと隣接した第3末端ベースユニットに位置する前記リザーバである第3末端リザーバ、および前記第2末端ベースユニットと隣接した第4末端ベースユニットに位置する前記リザーバである第4末端リザーバに位置することを特徴とする、請求項2に記載の試料分離装置。
- 前記複数のベースユニットのうち末端に位置しないベースユニットと直接的に連結され、前記ベースが折り畳まれる時に外部に少なくとも一部位置し、サンプルを注入するためのインジェクションベースユニットをさらに含むことを特徴とする、請求項1に記載の試料分離装置。
- 前記インジェクションベースユニットの少なくとも一面には、
前記インジェクションベースユニットの少なくとも一部領域に位置して処理対象試料の吸着を防止し、貯蔵空間または移動経路を区分するインジェクションベースユニットコーティング層、
前記複数のベースユニットのうち末端に位置しないベースユニットに含まれたリザーバと連結され、処理対象試料を投入するために濡らされるインジェクションリザーバ、および
前記コーティング層により領域が区分され、前記ベースユニットのリザーバと前記インジェクションリザーバを連結する処理対象試料の移動経路であるサンプルインジェクションチャネルを含むことを特徴とする、請求項4に記載の試料分離装置。 - 前記ベースは多孔質メンブレンを含み、前記ベースは前記複数のベースユニットが折り畳まれるように形成されていることを特徴とする、請求項1に記載の試料分離装置。
- スリップレイヤベース、前記スリップレイヤベースの少なくとも一部領域に位置するスリップレイヤコーティング層、および前記スリップレイヤコーティング層により領域が定義され、測定しようとする試料を少なくとも一部吸着して貯蔵するかまたは移動経路を提供するスリップレイヤリザーバを含み、前記ベースに含まれる少なくとも一部のベースユニットの間に挿入または除去が可能となるように備えられたスリップレイヤユニットをさらに含み、
前記スリップレイヤリザーバは、試料の投入のために処理対象となる試料または導電性液体で濡らされることを特徴とする、請求項1に記載の試料分離装置。 - 前記複数のリザーバは、処理対象となる試料または導電性液体で濡らされることを特徴とする、請求項1に記載の試料分離装置。
- 前記ベースユニットは、必要に応じて少なくとも一つを切り取って前記試料のターゲット物質または分離対象物質を選択的に得ることができるようにすることを特徴とする、請求項1に記載の試料分離装置。
- 前記コーティング層は、試料の吸着を防止するための疎水性物質を含み、物理的切断方式であるカッティングによって一定パターンの前記疎水性物質のコーティング膜を形成した後に前記ベースの両面に付着されるか、または前記疎水性物質を前記パターンに応じて塗布するパターニングによって形成されることを特徴とする、請求項1に記載の試料分離装置。
- 前記濾過層は多孔性メンブレンを含み、
前記濾過層の微細孔(Pore)の大きさは、前記収集対象物の大きさより大きく、前記分離対象物の大きさより小さいことを特徴とする、請求項1に記載の試料分離装置。 - 前記濾過層を圧着して前記微細孔の大きさを調節し、
前記濾過層は前記濾過層に加えられた圧力の強さまたは前記微細孔の大きさを表示する表示部をさらに含むことを特徴とする、請求項11に記載の試料分離装置。 - 前記複数の濾過層は前記複数のベースユニットが形成する前記収集対象物の移動経路上に位置して2以上の分離対象物を段階的に分離することを特徴とする、請求項1に記載の試料分離装置。
- 前記濾過層は前記複数のベースユニットのうち末端に位置しないベースユニットと直接的に連結され、
前記複数のベースユニットが折り畳まれる過程で前記収集対象物の移動経路の中間に前記複数の濾過層が挿入されるように形成されることを特徴とする、請求項1に記載の試料分離装置。
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KR10-2016-0124951 | 2016-09-28 | ||
KR1020160124951A KR101830758B1 (ko) | 2016-09-28 | 2016-09-28 | 시료 분리 장치 |
KR10-2017-0105825 | 2017-08-22 | ||
PCT/KR2017/009121 WO2018062686A1 (ko) | 2016-09-28 | 2017-08-22 | 종이접기 기반의 시료 분리 장치 |
KR1020170105825A KR101968319B1 (ko) | 2017-08-22 | 2017-08-22 | 여과층을 이용한 종이접기 기반의 시료 분리 장치 |
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KR102125414B1 (ko) * | 2018-10-25 | 2020-06-22 | 주식회사 켈스 | 대용량 생체 시료 농축 장치 및 그 제조 방법 |
KR102536164B1 (ko) * | 2020-10-30 | 2023-05-26 | 주식회사 엘지화학 | 시료 내 이온성 화합물의 분리 및 농축 시스템 및 이를 이용한 이온성 화합물의 분석 방법 |
CN117330552B (zh) * | 2023-11-27 | 2024-02-02 | 中国科学院烟台海岸带研究所 | 分子印迹传感纸芯片检测膝沟藻毒素1,4的方法 |
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JP2003066005A (ja) * | 2001-08-23 | 2003-03-05 | Kikuchi Jun | 細胞の電気泳動方法及び装置 |
EA010662B1 (ru) | 2004-08-13 | 2008-10-30 | Эгомедикаль Технологиз Аг | Тест-система для определения концентрации анализируемого вещества в физиологической или водной жидкости |
JP2006094822A (ja) | 2004-09-30 | 2006-04-13 | Matsushita Electric Ind Co Ltd | 微生物の分離法 |
CN101578520B (zh) | 2006-10-18 | 2015-09-16 | 哈佛学院院长等 | 基于形成图案的多孔介质的横向流动和穿过生物测定装置、及其制备方法和使用方法 |
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BRPI0913784A2 (pt) | 2008-09-30 | 2015-10-20 | Menai Medical Technologies Ltd | "sistema de medição de amostra, placa de amostragem, dispositivo de medição, adaptador, carregador de dados, método de produção da placa de amostragem, método de produção de uma folha contínua, folha contínua, aparelho, método para testar uma condição médica, e, kit de diagnóstico para testar uma condição médica" |
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JP2012018039A (ja) | 2010-07-07 | 2012-01-26 | Sony Corp | マイクロ流路と核酸ハイブリダイゼーションのためのマイクロチップとカラム及び装置並びに方法 |
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US20160146755A1 (en) | 2014-11-25 | 2016-05-26 | Board Of Regents, The University Of Texas System | Devices, systems, and methods for electrophoresis |
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