JP6870178B2 - 卵細胞質内注入法 - Google Patents
卵細胞質内注入法 Download PDFInfo
- Publication number
- JP6870178B2 JP6870178B2 JP2020024006A JP2020024006A JP6870178B2 JP 6870178 B2 JP6870178 B2 JP 6870178B2 JP 2020024006 A JP2020024006 A JP 2020024006A JP 2020024006 A JP2020024006 A JP 2020024006A JP 6870178 B2 JP6870178 B2 JP 6870178B2
- Authority
- JP
- Japan
- Prior art keywords
- injection
- tip
- pipette
- injection pipette
- egg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
- B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
- B25J7/00—Micromanipulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Robotics (AREA)
- Mechanical Engineering (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Microscoopes, Condenser (AREA)
- Manipulator (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019027726 | 2019-02-19 | ||
| JP2019027726 | 2019-02-19 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020130181A JP2020130181A (ja) | 2020-08-31 |
| JP2020130181A5 JP2020130181A5 (enExample) | 2020-11-12 |
| JP6870178B2 true JP6870178B2 (ja) | 2021-05-12 |
Family
ID=72145002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020024006A Active JP6870178B2 (ja) | 2019-02-19 | 2020-02-17 | 卵細胞質内注入法 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP6870178B2 (enExample) |
| WO (1) | WO2020171038A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112553057A (zh) * | 2020-12-11 | 2021-03-26 | 东蕴医疗科技(上海)有限公司 | 一种用于压电式单精子注射的显微操作针及其制作方法 |
| CN115181663B (zh) * | 2022-06-29 | 2025-12-19 | 北京理工大学 | 一种基于声场和磁场混合驱动的注射微机器人及控制方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4664097A (en) * | 1984-05-09 | 1987-05-12 | The Wistar Institute Of Anatomy & Biology | Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion |
| JPH0690770A (ja) * | 1991-03-29 | 1994-04-05 | Shimadzu Corp | マイクロマニピュレータ用微小器具 |
| JP2002204945A (ja) * | 2000-10-16 | 2002-07-23 | Ngk Insulators Ltd | マイクロピペット、分注装置及びバイオチップの製造方法 |
| JP2004325836A (ja) * | 2003-04-25 | 2004-11-18 | Suruga Seiki Kk | マイクロマニピュレータ |
| JP2005074586A (ja) * | 2003-09-01 | 2005-03-24 | Seoul National Univ Industry Foundation | 改選された卵細胞質内精子注入法(Animprovedmethodofintracytoplasmicsperminjection) |
| JP5051617B2 (ja) * | 2007-12-17 | 2012-10-17 | 富士電機株式会社 | 遠心分離方法及び遠心分離装置 |
| EP3492926B1 (en) * | 2016-07-26 | 2021-06-23 | Konica Minolta, Inc. | Pipette tip, liquid delivery method and liquid delivery system |
| JP6862624B2 (ja) * | 2018-03-13 | 2021-04-21 | プライムテック株式会社 | 卵細胞質内注入法 |
-
2020
- 2020-02-17 JP JP2020024006A patent/JP6870178B2/ja active Active
- 2020-02-18 WO PCT/JP2020/006168 patent/WO2020171038A1/ja not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JP2020130181A (ja) | 2020-08-31 |
| WO2020171038A1 (ja) | 2020-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Giandomenico et al. | Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development | |
| Yoshida et al. | Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) | |
| Cheers et al. | Rapid microinjection of fertilized eggs | |
| US20080213899A1 (en) | Rotationally Oscillating Injector | |
| JP6870178B2 (ja) | 卵細胞質内注入法 | |
| JP6862624B2 (ja) | 卵細胞質内注入法 | |
| Chambers | New apparatus and methods for the dissection and injection of living cells | |
| Bogliotti et al. | Laser-assisted cytoplasmic microinjection in livestock zygotes | |
| JP2019154307A5 (enExample) | ||
| CN1441652A (zh) | 微流控通道胚胎和/或卵母细胞处理,分析和生物评价 | |
| JP2020130181A5 (enExample) | ||
| Ward et al. | Intracytoplasmic sperm injection in mice | |
| Lane et al. | Microinjection of Xenopus tropicalis embryos | |
| CN105631240A (zh) | 一种基于微管吸持法的批量测量细胞透明带杨氏模量方法 | |
| CN112553057A (zh) | 一种用于压电式单精子注射的显微操作针及其制作方法 | |
| Ogura | Cloning mice | |
| CN110628591A (zh) | 一种胚胎活检样本的转移方法 | |
| Kline | Quantitative microinjection of mouse oocytes and eggs | |
| CN217838941U (zh) | 单精子注射针和单精子显微注射系统 | |
| Shull et al. | Manipulation of single neural stem cells and neurons in brain slices using robotic microinjection | |
| KR100505124B1 (ko) | 개선된 난세포질내 정자 주입법 | |
| WO2009088384A1 (en) | Controlled flow rate micro-pipettes | |
| CN211339458U (zh) | 一种体外卵胞浆内单精子显微注射操作装置 | |
| CN223974091U (zh) | 一种剥离针管装置 | |
| Khoo et al. | Fate-mapping technique: targeted whole-embryo electroporation of DNA constructs into the germ layers of mouse embryos 7-7.5 days post-coitum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200219 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200928 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200928 |
|
| A871 | Explanation of circumstances concerning accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A871 Effective date: 20200928 |
|
| A975 | Report on accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A971005 Effective date: 20201015 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20201117 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210115 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210316 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210317 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6870178 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |