JP6870178B2 - 卵細胞質内注入法 - Google Patents

卵細胞質内注入法 Download PDF

Info

Publication number
JP6870178B2
JP6870178B2 JP2020024006A JP2020024006A JP6870178B2 JP 6870178 B2 JP6870178 B2 JP 6870178B2 JP 2020024006 A JP2020024006 A JP 2020024006A JP 2020024006 A JP2020024006 A JP 2020024006A JP 6870178 B2 JP6870178 B2 JP 6870178B2
Authority
JP
Japan
Prior art keywords
injection
tip
pipette
injection pipette
egg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020024006A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020130181A (ja
JP2020130181A5 (enExample
Inventor
正樹 岩元
正樹 岩元
径子 高山
径子 高山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PRIMETECH CORPORATION
Original Assignee
PRIMETECH CORPORATION
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PRIMETECH CORPORATION filed Critical PRIMETECH CORPORATION
Publication of JP2020130181A publication Critical patent/JP2020130181A/ja
Publication of JP2020130181A5 publication Critical patent/JP2020130181A5/ja
Application granted granted Critical
Publication of JP6870178B2 publication Critical patent/JP6870178B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B25HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25JMANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J7/00Micromanipulators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/32Micromanipulators structurally combined with microscopes

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Robotics (AREA)
  • Mechanical Engineering (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Microscoopes, Condenser (AREA)
  • Manipulator (AREA)
JP2020024006A 2019-02-19 2020-02-17 卵細胞質内注入法 Active JP6870178B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019027726 2019-02-19
JP2019027726 2019-02-19

Publications (3)

Publication Number Publication Date
JP2020130181A JP2020130181A (ja) 2020-08-31
JP2020130181A5 JP2020130181A5 (enExample) 2020-11-12
JP6870178B2 true JP6870178B2 (ja) 2021-05-12

Family

ID=72145002

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020024006A Active JP6870178B2 (ja) 2019-02-19 2020-02-17 卵細胞質内注入法

Country Status (2)

Country Link
JP (1) JP6870178B2 (enExample)
WO (1) WO2020171038A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112553057A (zh) * 2020-12-11 2021-03-26 东蕴医疗科技(上海)有限公司 一种用于压电式单精子注射的显微操作针及其制作方法
CN115181663B (zh) * 2022-06-29 2025-12-19 北京理工大学 一种基于声场和磁场混合驱动的注射微机器人及控制方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664097A (en) * 1984-05-09 1987-05-12 The Wistar Institute Of Anatomy & Biology Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion
JPH0690770A (ja) * 1991-03-29 1994-04-05 Shimadzu Corp マイクロマニピュレータ用微小器具
JP2002204945A (ja) * 2000-10-16 2002-07-23 Ngk Insulators Ltd マイクロピペット、分注装置及びバイオチップの製造方法
JP2004325836A (ja) * 2003-04-25 2004-11-18 Suruga Seiki Kk マイクロマニピュレータ
JP2005074586A (ja) * 2003-09-01 2005-03-24 Seoul National Univ Industry Foundation 改選された卵細胞質内精子注入法(Animprovedmethodofintracytoplasmicsperminjection)
JP5051617B2 (ja) * 2007-12-17 2012-10-17 富士電機株式会社 遠心分離方法及び遠心分離装置
EP3492926B1 (en) * 2016-07-26 2021-06-23 Konica Minolta, Inc. Pipette tip, liquid delivery method and liquid delivery system
JP6862624B2 (ja) * 2018-03-13 2021-04-21 プライムテック株式会社 卵細胞質内注入法

Also Published As

Publication number Publication date
JP2020130181A (ja) 2020-08-31
WO2020171038A1 (ja) 2020-08-27

Similar Documents

Publication Publication Date Title
Giandomenico et al. Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development
Yoshida et al. Piezo-actuated mouse intracytoplasmic sperm injection (ICSI)
Cheers et al. Rapid microinjection of fertilized eggs
US20080213899A1 (en) Rotationally Oscillating Injector
JP6870178B2 (ja) 卵細胞質内注入法
JP6862624B2 (ja) 卵細胞質内注入法
Chambers New apparatus and methods for the dissection and injection of living cells
Bogliotti et al. Laser-assisted cytoplasmic microinjection in livestock zygotes
JP2019154307A5 (enExample)
CN1441652A (zh) 微流控通道胚胎和/或卵母细胞处理,分析和生物评价
JP2020130181A5 (enExample)
Ward et al. Intracytoplasmic sperm injection in mice
Lane et al. Microinjection of Xenopus tropicalis embryos
CN105631240A (zh) 一种基于微管吸持法的批量测量细胞透明带杨氏模量方法
CN112553057A (zh) 一种用于压电式单精子注射的显微操作针及其制作方法
Ogura Cloning mice
CN110628591A (zh) 一种胚胎活检样本的转移方法
Kline Quantitative microinjection of mouse oocytes and eggs
CN217838941U (zh) 单精子注射针和单精子显微注射系统
Shull et al. Manipulation of single neural stem cells and neurons in brain slices using robotic microinjection
KR100505124B1 (ko) 개선된 난세포질내 정자 주입법
WO2009088384A1 (en) Controlled flow rate micro-pipettes
CN211339458U (zh) 一种体外卵胞浆内单精子显微注射操作装置
CN223974091U (zh) 一种剥离针管装置
Khoo et al. Fate-mapping technique: targeted whole-embryo electroporation of DNA constructs into the germ layers of mouse embryos 7-7.5 days post-coitum

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200219

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200928

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200928

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20200928

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20201015

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201117

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210115

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210316

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210317

R150 Certificate of patent or registration of utility model

Ref document number: 6870178

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250