JP6850944B2 - ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法並びに増殖の早いヒト間葉系幹細胞の細胞集団 - Google Patents

ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法並びに増殖の早いヒト間葉系幹細胞の細胞集団 Download PDF

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JP6850944B2
JP6850944B2 JP2016538462A JP2016538462A JP6850944B2 JP 6850944 B2 JP6850944 B2 JP 6850944B2 JP 2016538462 A JP2016538462 A JP 2016538462A JP 2016538462 A JP2016538462 A JP 2016538462A JP 6850944 B2 JP6850944 B2 JP 6850944B2
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mesenchymal stem
cells
stem cells
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JPWO2016017795A1 (ja
JPWO2016017795A6 (ja
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有未 伊谷
有未 伊谷
岡野 栄之
栄之 岡野
洋 馬渕
洋 馬渕
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Purec Co Ltd
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JP2016538462A 2014-08-01 2015-07-31 ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法並びに増殖の早いヒト間葉系幹細胞の細胞集団 Active JP6850944B2 (ja)

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JP2019190347A JP6932390B2 (ja) 2014-08-01 2019-10-17 ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法、増殖の早いヒト間葉系幹細胞の細胞集団、並びに、増殖の早いヒト間葉系幹細胞を特異的に認識するモノクローナル抗体

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JP2014157367 2014-08-01
PCT/JP2015/071770 WO2016017795A1 (ja) 2014-08-01 2015-07-31 ヒト間葉系幹細胞の品質評価方法、及び、そのためのモノクローナル抗体

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JP2019190347A Active JP6932390B2 (ja) 2014-08-01 2019-10-17 ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法、増殖の早いヒト間葉系幹細胞の細胞集団、並びに、増殖の早いヒト間葉系幹細胞を特異的に認識するモノクローナル抗体
JP2021040712A Pending JP2021100414A (ja) 2014-08-01 2021-03-12 ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法、増殖の早いヒト間葉系幹細胞の細胞集団、並びに、増殖の早いヒト間葉系幹細胞を特異的に認識するモノクローナル抗体

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JP2021040712A Pending JP2021100414A (ja) 2014-08-01 2021-03-12 ヒト間葉系幹細胞の品質評価方法、ヒト間葉系幹細胞の分離、選別及び培養方法、増殖の早いヒト間葉系幹細胞の細胞集団、並びに、増殖の早いヒト間葉系幹細胞を特異的に認識するモノクローナル抗体

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025075210A1 (ja) 2023-10-02 2025-04-10 PuREC株式会社 Aiを用いた画像解析による幹細胞の品質管理方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11293065B2 (en) 2016-03-14 2022-04-05 Aelan Cell Technologies, Inc. Compositions and methods for the quality control of stem cell preparations
WO2020205732A1 (en) * 2019-03-29 2020-10-08 The Johns Hopkins University Grid of responses indicating drug sensitivity
CN112094804B (zh) * 2019-06-18 2024-05-14 中国医学科学院基础医学研究所 一种异质性干细胞群、其制备方法及用途
US12460182B2 (en) 2019-08-19 2025-11-04 Cellaxia Inc. Cell-containing pharmaceutical composition
WO2021144995A1 (ja) * 2020-01-16 2021-07-22 PuREC株式会社 高純度間葉系幹細胞
US20240018588A1 (en) * 2020-12-07 2024-01-18 Samsung Life Public Welfare Foundation Method for selecting mesenchymal stem cells having improved self-maintenance ability, and mesenchymal stem cells selected thereby
TW202246491A (zh) * 2021-01-29 2022-12-01 國立大學法人北海道大學 椎間盤再生用組成物
WO2022177032A1 (ja) 2021-02-19 2022-08-25 PuREC株式会社 骨疾患治療用医薬組成物
JP7368679B2 (ja) * 2021-09-01 2023-10-25 イミュニティリサーチ株式会社 細胞集団同定システム、方法、およびプログラム
CN116486909A (zh) * 2022-01-14 2023-07-25 天士力干细胞产业平台有限公司 一种干细胞质量评价系统
WO2023189485A1 (ja) 2022-03-31 2023-10-05 株式会社サイト-ファクト 間葉系幹細胞の品質評価方法
CN120322547A (zh) * 2023-10-26 2025-07-15 京东方科技集团股份有限公司 细胞培养方法、筛选方法以及间充质干细胞
WO2025187668A1 (ja) * 2024-03-06 2025-09-12 国立研究開発法人産業技術総合研究所 骨分化能判定方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004094641A2 (en) 2003-04-16 2004-11-04 Wyeth A novel method of modulating bone-realted activity
CA2628221A1 (en) * 2005-10-31 2007-05-10 Oncomed Pharmaceuticals, Inc. Anti-frizzled receptor antibodies for treating cancer
PE20071309A1 (es) * 2006-02-17 2008-02-13 Wyeth Corp Anticuerpos para la modulacion de formacion de huesos
WO2009011546A2 (en) 2007-07-16 2009-01-22 Catholic University Industry Academic Cooperation Foundation Method for promoting the self-renewal of adult stem cells using mesenchymal stromal cells
JP2009060840A (ja) * 2007-09-06 2009-03-26 Keio Gijuku ヒト間葉系幹細胞濃縮方法
US20130209415A1 (en) 2010-06-03 2013-08-15 The Board Of Trustees Of The Leland Stanford Junior University Purified compositions of cardiovascular progenitor cells
US20120087868A1 (en) 2010-10-08 2012-04-12 Gabriele Todd Nanoparticle-loaded cells
JP2013066414A (ja) * 2011-09-22 2013-04-18 National Institute Of Advanced Industrial Science & Technology 胃前駆細胞の表面マーカー
JP6463029B2 (ja) * 2013-08-02 2019-01-30 有未 伊谷 ヒト間葉系幹細胞を特異的に認識するモノクローナル抗体並びにこれを用いたヒト間葉系幹細胞の分離及び/または品質評価を行う方法
JP5924750B2 (ja) 2014-05-01 2016-05-25 iHeart Japan株式会社 Cd82陽性心筋前駆細胞

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025075210A1 (ja) 2023-10-02 2025-04-10 PuREC株式会社 Aiを用いた画像解析による幹細胞の品質管理方法
KR20260042258A (ko) 2023-10-02 2026-03-30 퓨렉 가부시키가이샤 Ai 를 이용한 화상 해석에 의한 줄기세포의 품질 관리 방법

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US20210230553A1 (en) 2021-07-29
CA2954245C (en) 2023-07-25
CA2954245A1 (en) 2016-02-04
JPWO2016017795A1 (ja) 2017-06-22
EP3176253A4 (en) 2018-03-21
SG10202005439XA (en) 2020-07-29
EP3176253B1 (en) 2022-08-24
US11441123B2 (en) 2022-09-13
AU2015297347B2 (en) 2021-05-27
JP2020072662A (ja) 2020-05-14
WO2016017795A1 (ja) 2016-02-04
US20170204374A1 (en) 2017-07-20
JP6932390B2 (ja) 2021-09-08
ES2927112T3 (es) 2022-11-02
EP3176253A1 (en) 2017-06-07
JP2021100414A (ja) 2021-07-08
SG11201610795VA (en) 2017-02-27

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