JP6837667B2 - 誘導ラマン散乱顕微鏡装置および誘導ラマン散乱計測方法 - Google Patents
誘導ラマン散乱顕微鏡装置および誘導ラマン散乱計測方法 Download PDFInfo
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Description
所定光周波数の第1光パルスを第1繰り返し周波数で出力する第1光パルス生成部と、
前記第1光パルスとは異なる光周波数の第2光パルスを整数倍すると前記第1繰り返し周波数となる第2繰り返し周波数で出力する第2光パルス生成部と、
前記第1光パルスと前記第2光パルスとを同期させて試料に照射した際の透過光および/または散乱光に含まれる前記第1繰り返し周波数の前記所定光周波数の光パルスの光強度を検出する光強度検出部と、
を備える誘導ラマン散乱顕微鏡装置であって、
前記第2光パルス生成部は、
複数の光周波数の光を含む所定光パルスを出力する光源部と、
前記所定光パルスを分光すると共に分光した光パルスのうち予め定めた所定数の異なる光周波数の光パルスを前記第2繰り返し周波数で出力する分光調整部と、
前記分光調整部からの光パルスを結合する光結合部と、
を備える、
ことを特徴とする。
所定光周波数の第1光パルスを第1繰り返し周波数で試料に照射すると共に前記第1光パルスとは異なる光周波数の第2光パルスを整数倍すると前記第1繰り返し周波数となる第2繰り返し周波数で前記第1光パルスに同期させて照射し、前記第1光パルスと前記第2光パルスとの照射に対して前記試料の透過光および/または散乱光に含まれる前記第1繰り返し周波数の前記所定光周波数の光パルスを検出光パルス列として検出する誘導ラマン散乱計測方法であって、
前記第2光パルスは、複数の光周波数の光を含む所定光パルスを分光すると共に分光した光パルスのうち予め定めた所定数の異なる光周波数の光パルスを前記第2繰り返し周波数で出力するように調整し、前記調整された光パルスを結合することにより生成される、ことを特徴とする。
Claims (7)
- 所定光周波数の第1光パルスを第1繰り返し周波数で出力する第1光パルス生成部と、
前記第1光パルスとは異なる光周波数の第2光パルスを整数倍すると前記第1繰り返し周波数となる第2繰り返し周波数で出力する第2光パルス生成部と、
前記第1光パルスと前記第2光パルスとを同期させて試料に照射した際の透過光および/または散乱光に含まれる前記第1繰り返し周波数の前記所定光周波数の光パルスの光強度を検出する光強度検出部と、
を備える誘導ラマン散乱顕微鏡装置であって、
前記第2光パルス生成部は、
複数の光周波数の光を含む所定光パルスを出力する光源部と、
前記所定光パルスを分光すると共に分光した光パルスのうち予め定めた所定数の異なる光周波数の光パルスを前記第2繰り返し周波数で出力する分光調整部と、
前記分光調整部からの光パルスを結合する光結合部と、
を備える、
ことを特徴とする誘導ラマン散乱顕微鏡装置。 - 請求項1記載の誘導ラマン散乱顕微鏡装置であって、
前記光源部は、前記所定光パルスを、前記所定数倍すると前記第2繰り返し周波数となる繰り返し周波数で出力する光源である、
誘導ラマン散乱顕微鏡装置。 - 請求項1記載の誘導ラマン散乱顕微鏡装置であって、
前記光源部は、前記所定光パルスが前記第2繰り返し周波数で所定連続数だけ連続する所定光パルス列を、前記所定数に前記所定連続数を乗じた数との積が前記第2繰り返し周波数となる繰り返し周波数で出力する光源部である、
誘導ラマン散乱顕微鏡装置。 - 請求項3記載の誘導ラマン散乱顕微鏡装置であって、
前記光源部は、
前記第2繰り返し周波数で前記所定光パルスを出力する光源と、
前記光源からの前記第2繰り返し周波数の前記所定光パルスの光パルス列から、前記所定数を前記所定連続数を乗じた数との積が前記第2繰り返し周波数となる繰り返し周波数で前記所定光パルス列が出力されるように残余の光パルスの強度を値0とする光強度変調部と、
を備える、
誘導ラマン散乱顕微鏡装置。 - 請求項1ないし4のうちのいずれか1つの請求項に記載の誘導ラマン散乱顕微鏡装置であって、
前記第2光パルス生成部は、前記光結合部からの光パルスの強度を増幅して出力する強度増幅部を備える、
誘導ラマン散乱顕微鏡装置。 - 請求項1ないし5のうちのいずれか1つの請求項に記載の誘導ラマン散乱顕微鏡装置であって、
前記光強度検出部からの検出信号に対して、前記第2繰り返し周波数の1/2の周波数をカットオフ周波数とするローパスフィルタと、前記第2繰り返し周波数の1/5〜1/10の周波数をカットオフ周波数とするハイパスフィルタと、少なくとも前記第1繰り返し周波数のノッチフィルタと、を施すフィルタ処理部と、
前記フィルタ処理部からの出力に対して、所定サンプリング周期でデジタイズして強度変調を計測する強度変調計測部と、
を備える、
誘導ラマン散乱顕微鏡装置。 - 所定光周波数の第1光パルスを第1繰り返し周波数で試料に照射すると共に前記第1光パルスとは異なる光周波数の第2光パルスを整数倍すると前記第1繰り返し周波数となる第2繰り返し周波数で前記第1光パルスに同期させて照射し、前記第1光パルスと前記第2光パルスとの照射に対して前記試料の透過光および/または散乱光に含まれる前記第1繰り返し周波数の前記所定光周波数の光パルスを検出光パルス列として検出する誘導ラマン散乱計測方法であって、
前記第2光パルスは、複数の光周波数の光を含む所定光パルスを分光すると共に分光した光パルスのうち予め定めた所定数の異なる光周波数の光パルスを前記第2繰り返し周波数で出力するように調整し、前記調整された光パルスを結合することにより生成される、
ことを特徴とする誘導ラマン散乱計測方法。
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JP5697584B2 (ja) * | 2011-11-25 | 2015-04-08 | キヤノン株式会社 | 誘導ラマン散乱計測装置および誘導ラマン散乱計測方法 |
JP5697699B2 (ja) | 2013-03-08 | 2015-04-08 | キヤノン株式会社 | ラマン散乱計測装置およびラマン散乱計測方法 |
FR3003949B1 (fr) * | 2013-03-26 | 2015-05-01 | Univ Aix Marseille | Dispositif et methode de detection raman stimulee. |
EP2982968A4 (en) * | 2013-04-05 | 2016-11-30 | Nikon Corp | CELL OBSERVATION METHOD, CELL OBSERVATION DEVICE, CELL OBSERVATION PROGRAM, CELL SHEET MANUFACTURING METHOD, AND CELL SHEET MANUFACTURING DEVICE |
US20150204790A1 (en) | 2014-01-23 | 2015-07-23 | Canon Kabushiki Kaisha | Stimulated raman scattering measurement apparatus |
JP2015158482A (ja) * | 2014-01-23 | 2015-09-03 | キヤノン株式会社 | 誘導ラマン散乱計測装置 |
EP3123128B1 (en) * | 2014-03-24 | 2020-04-29 | Optiqgain Ltd. | A system for a stimulated raman scattering (srs) spectrophotometer and a method of use thereof |
JP5917664B2 (ja) * | 2014-11-25 | 2016-05-18 | キヤノン株式会社 | 誘導ラマン散乱計測装置および誘導ラマン散乱計測方法 |
CN108139267B (zh) * | 2015-10-09 | 2021-06-08 | 徕卡显微系统复合显微镜有限公司 | Srs成像的动态锁定检测带宽 |
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2017
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- 2017-02-09 US US16/076,389 patent/US11340171B2/en active Active
- 2017-02-09 WO PCT/JP2017/004728 patent/WO2017138606A1/ja active Application Filing
- 2017-02-09 JP JP2017566996A patent/JP6837667B2/ja active Active
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WO2017138606A1 (ja) | 2017-08-17 |
US11340171B2 (en) | 2022-05-24 |
EP3415898A4 (en) | 2019-09-11 |
EP3415898A1 (en) | 2018-12-19 |
US20190137401A1 (en) | 2019-05-09 |
JPWO2017138606A1 (ja) | 2018-12-06 |
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