JP6773268B2 - 多能性幹細胞を所望の細胞型へ分化する方法 - Google Patents

多能性幹細胞を所望の細胞型へ分化する方法 Download PDF

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JP6773268B2
JP6773268B2 JP2017505381A JP2017505381A JP6773268B2 JP 6773268 B2 JP6773268 B2 JP 6773268B2 JP 2017505381 A JP2017505381 A JP 2017505381A JP 2017505381 A JP2017505381 A JP 2017505381A JP 6773268 B2 JP6773268 B2 JP 6773268B2
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JPWO2016143826A1 (ja
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実 洪
実 洪
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Keio University
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JP2021099810A Active JP7403841B2 (ja) 2015-03-09 2021-06-16 多能性幹細胞を所望の細胞型へ分化する方法
JP2023063437A Pending JP2023085519A (ja) 2015-03-09 2023-04-10 多能性幹細胞を所望の細胞型へ分化する方法
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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016143826A1 (ja) 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
JP7370529B2 (ja) 2015-08-31 2023-10-30 剛士 田邊 多能性幹細胞製造システム、幹細胞の誘導方法、幹細胞の浮遊培養方法、幹細胞の浮遊培養器、人工多能性幹細胞の作製方法、及び動物細胞から特定の体細胞を作製する方法
HK1252090A1 (zh) * 2015-08-31 2019-05-17 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
WO2017073692A1 (ja) * 2015-10-30 2017-05-04 国立大学法人鳥取大学 メチル化関連酵素ｈａｔ１とｋａｔ８の阻害薬
US20190078053A1 (en) * 2016-03-15 2019-03-14 Keio University Method for producing cardiac precursor cell and myocardial cell from pluripotent stem cell
US11845960B2 (en) 2016-09-12 2023-12-19 President And Fellows Of Harvard College Transcription factors controlling differentiation of stem cells
GB201619876D0 (en) 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
US20190352604A1 (en) * 2017-02-24 2019-11-21 Koji Tanabe Nerve cell production method
JP2018143239A (ja) * 2017-03-01 2018-09-20 エリクサジェン，エルエルシー． 多能性幹細胞を所望の細胞型へ効率的に分化する方法
EP3717654A4 (en) 2017-12-01 2021-11-10 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR PRODUCING OLIGODENDROCYTE PRECURSOR CELLS
US11788131B2 (en) 2018-04-06 2023-10-17 President And Fellows Of Harvard College Methods of identifying combinations of transcription factors
WO2020223674A2 (en) * 2019-05-01 2020-11-05 Board Of Regents Of The University Of Texas System Methods and compositions for regulating adipogenesis
MX2021014456A (es) * 2019-05-27 2022-01-06 Univ Muenster Westfaelische Wilhelms Generacion rapida y deterministica de microglia a partir de celulas madre pluripotentes humanas.
WO2020243392A1 (en) 2019-05-31 2020-12-03 President And Fellows Of Harvard College Sox9-induced oligodendrocyte progenitor cells
KR20220047623A (ko) * 2019-08-19 2022-04-18 듀크 유니버시티 세포 유형 운명 특정화의 조절인자를 확인하기 위한 조성물 및 방법
JP7770028B2 (ja) * 2019-11-25 2025-11-14 アスガード セラピューティクス エービー 細胞を抗原提示に有能な樹状細胞２型に初期化するための組成物、その方法及び使用
JP2023516484A (ja) 2020-03-11 2023-04-19 ビット バイオ リミテッド 肝細胞作製方法
CN113652402A (zh) * 2020-05-12 2021-11-16 再康医药科技(上海)有限公司 一种诱导胶质细胞转分化为功能性神经元的方法及其应用
CN112626023B (zh) * 2021-01-12 2023-08-04 中国人民解放军海军军医大学 脊髓olig2+神经祖细胞诱导及自我更新培养体系、诱导方法及应用
TW202309268A (zh) * 2021-05-07 2023-03-01 安斯泰來再生醫藥協會 產生成熟肝細胞的方法
WO2022239724A1 (en) 2021-05-10 2022-11-17 Okinawa Institute Of Science And Technology School Corporation A modified rhabdovirus having a reduced toxicity
JP7306745B2 (ja) * 2021-09-16 2023-07-11 株式会社ユニバーサルエンターテインメント 遊技機
JP7268911B2 (ja) * 2021-09-16 2023-05-08 株式会社ユニバーサルエンターテインメント 遊技機
JP7306746B2 (ja) * 2021-09-16 2023-07-11 株式会社ユニバーサルエンターテインメント 遊技機
WO2024091801A2 (en) * 2022-10-13 2024-05-02 President And Fellows Of Harvard College Methods and compositions for inducing cell differentiation
WO2025254221A1 (ja) * 2024-06-07 2025-12-11 国立大学法人九州大学 形質転換幹細胞、神経細胞、及び、形質転換幹細胞由来の神経細胞の作製方法
JP7755342B1 (ja) * 2024-10-02 2025-10-16 株式会社Dioseve 卵子様細胞を製造する方法、及び卵子様細胞

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7344857B2 (en) * 1996-09-27 2008-03-18 California Institute Of Technology Neurogenin
WO2001094549A2 (en) * 2000-06-05 2001-12-13 The Burnham Institute Methods of differentiating and protecting cells by modulating the p38/mef2 pathway
JP2004016109A (ja) * 2002-06-17 2004-01-22 Nagoya Industrial Science Research Inst 軟骨細胞様細胞、及びその作製方法
AU2003273332A1 (en) 2002-09-26 2004-04-19 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
JP4706208B2 (ja) * 2004-08-27 2011-06-22 株式会社アイル 造血幹細胞の製造方法
CA2644995A1 (en) * 2006-03-07 2007-09-13 The Trustees Of The University Of Pennsylvania Random rnai libraries, methods of generating same, and screening methods utilizing same
EP2495320B1 (en) * 2009-10-28 2019-02-27 Japan Health Sciences Foundation Method for induction of differentiation of stem cells into hepatocytes
US8481317B2 (en) 2010-04-13 2013-07-09 Cellular Dynamics International, Inc. Hepatocyte production by forward programming
WO2012109208A2 (en) 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming
US9770471B2 (en) 2011-08-17 2017-09-26 President And Fellows Of Harvard College Conversion of somatic cells into functional spinal motor neurons, and methods and uses thereof
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
EP2841563B1 (en) * 2012-04-24 2019-06-12 Dan S. Kaufman Method for developing natural killer cells from stem cells
JP5970245B2 (ja) * 2012-06-06 2016-08-17 公益財団法人ヒューマンサイエンス振興財団 幹細胞から肝細胞への分化誘導方法
WO2014069479A1 (ja) * 2012-10-29 2014-05-08 学校法人埼玉医科大学 分化多能性幹細胞の製造方法
US9938499B2 (en) * 2013-03-13 2018-04-10 Wisconsin Alumni Research Foundation Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
US10519421B2 (en) 2013-03-21 2019-12-31 Kyoto University Induction of motor neurons from pluripotent stem cells
WO2016143826A1 (ja) 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法

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JP6903355B2 (ja) 2021-07-14
WO2016143826A1 (ja) 2016-09-15
JP2020182452A (ja) 2020-11-12
JP7403841B2 (ja) 2023-12-25
US11891621B2 (en) 2024-02-06
JP2023085519A (ja) 2023-06-20
US20210024887A1 (en) 2021-01-28
JP2025032187A (ja) 2025-03-11
JP2021151252A (ja) 2021-09-30
US20180127714A1 (en) 2018-05-10
US20240124836A1 (en) 2024-04-18
US10836997B2 (en) 2020-11-17

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