MX2021014456A - Generacion rapida y deterministica de microglia a partir de celulas madre pluripotentes humanas. - Google Patents

Generacion rapida y deterministica de microglia a partir de celulas madre pluripotentes humanas.

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Publication number
MX2021014456A
MX2021014456A MX2021014456A MX2021014456A MX2021014456A MX 2021014456 A MX2021014456 A MX 2021014456A MX 2021014456 A MX2021014456 A MX 2021014456A MX 2021014456 A MX2021014456 A MX 2021014456A MX 2021014456 A MX2021014456 A MX 2021014456A
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Mexico
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microglia
stem cells
present
rapid
pluripotent stem
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MX2021014456A
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English (en)
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Matthias Pawlowski
Anna Martina Speicher
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Univ Muenster Westfaelische Wilhelms
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Publication of MX2021014456A publication Critical patent/MX2021014456A/es

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Abstract

La presente invención se relaciona con un método para la producción de microglía a partir de células madre que comprende los pasos de a) inserción dirigida de una secuencia de nucleótidos que codifica para una proteína reguladora transcripcional en un primer sitio de puerto genómico seguro; y b) inserción dirigida en la secuencia codificadora del factor de transcripción PU.1 (SEQ ID NO: 1) en un segundo sitio de puerto genómico seguro, donde el gen se une operativamente a un promotor inducible, el cual es regulado por la proteína reguladora transcripcional; expresión de PU.1 (SEQ ID NO: 2); y cultivar las células madre recibidas de los pasos a) y b) con exposición a al menos un factor de crecimiento o molécula pequeña que imita la señalización durante al menos una etapa del desarrollo embriónico de la microglía o proliferación, diferenciación o polarización de microglía adulta. Además, la presente invención se relaciona con la microglía obtenida por los métodos de la presente invención y varios usos de la misma.
MX2021014456A 2019-05-27 2020-05-27 Generacion rapida y deterministica de microglia a partir de celulas madre pluripotentes humanas. MX2021014456A (es)

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PCT/EP2020/064649 WO2020239807A1 (en) 2019-05-27 2020-05-27 Rapid and deterministic generation of microglia from human pluripotent stem cells

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CN117813385A (zh) * 2021-07-06 2024-04-02 庆应义塾 小胶质细胞祖细胞及小胶质细胞的制造方法、所制造的小胶质细胞祖细胞和小胶质细胞
WO2023102471A1 (en) * 2021-12-01 2023-06-08 Yale University Human cortical organoids with engineered microglia-like cells
WO2023135318A1 (en) * 2022-01-17 2023-07-20 Westfälische Wilhelms-Universität Münster Method for expression of a transgene of interest from neural precursor cells
JP2023140135A (ja) * 2022-03-22 2023-10-04 株式会社リコー ミクログリアの製造方法

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WO2016210313A1 (en) * 2015-06-24 2016-12-29 Whitehead Institute For Biomedical Research Culture medium for generating microglia from pluripotent stem cells and related methods
EP3423564A4 (en) * 2016-03-03 2019-08-21 New York Stem Cell Foundation, Inc. MICROGLIAL CELLS DERIVED FROM PLURIPOTENT STEM CELLS AND METHODS OF PREPARATION AND USE THEREOF
WO2018185709A1 (en) * 2017-04-05 2018-10-11 Centro De Neurociencias E Biologia Celular Compositions for reprogramming cells into dendritic cells or antigen presenting cells, methods and uses thereof
WO2018204262A1 (en) * 2017-05-01 2018-11-08 President And Fellows Of Harvard College Transcription factors controlling differentiation of stem cells
CN110205295B (zh) * 2019-06-25 2021-10-29 中国科学院动物研究所 一种多能干细胞诱导产生小胶质细胞的方法及试剂盒
CN117813385A (zh) * 2021-07-06 2024-04-02 庆应义塾 小胶质细胞祖细胞及小胶质细胞的制造方法、所制造的小胶质细胞祖细胞和小胶质细胞

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SG11202112475QA (en) 2021-12-30
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JP2022534260A (ja) 2022-07-28
WO2020239807A1 (en) 2020-12-03

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