JP6773268B2 - 多能性幹細胞を所望の細胞型へ分化する方法 - Google Patents

多能性幹細胞を所望の細胞型へ分化する方法 Download PDF

Info

Publication number
JP6773268B2
JP6773268B2 JP2017505381A JP2017505381A JP6773268B2 JP 6773268 B2 JP6773268 B2 JP 6773268B2 JP 2017505381 A JP2017505381 A JP 2017505381A JP 2017505381 A JP2017505381 A JP 2017505381A JP 6773268 B2 JP6773268 B2 JP 6773268B2
Authority
JP
Japan
Prior art keywords
stem cells
transcription factors
pluripotent stem
cells
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017505381A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2016143826A1 (ja
Inventor
実 洪
実 洪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keio University
Original Assignee
Keio University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keio University filed Critical Keio University
Publication of JPWO2016143826A1 publication Critical patent/JPWO2016143826A1/ja
Priority to JP2020075056A priority Critical patent/JP6903355B2/ja
Application granted granted Critical
Publication of JP6773268B2 publication Critical patent/JP6773268B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Neurosurgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2017505381A 2015-03-09 2016-03-09 多能性幹細胞を所望の細胞型へ分化する方法 Active JP6773268B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2020075056A JP6903355B2 (ja) 2015-03-09 2020-04-20 多能性幹細胞を所望の細胞型へ分化する方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015046318 2015-03-09
JP2015046318 2015-03-09
PCT/JP2016/057420 WO2016143826A1 (ja) 2015-03-09 2016-03-09 多能性幹細胞を所望の細胞型へ分化する方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020075056A Division JP6903355B2 (ja) 2015-03-09 2020-04-20 多能性幹細胞を所望の細胞型へ分化する方法

Publications (2)

Publication Number Publication Date
JPWO2016143826A1 JPWO2016143826A1 (ja) 2018-02-15
JP6773268B2 true JP6773268B2 (ja) 2020-10-21

Family

ID=56879159

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2017505381A Active JP6773268B2 (ja) 2015-03-09 2016-03-09 多能性幹細胞を所望の細胞型へ分化する方法
JP2020075056A Active JP6903355B2 (ja) 2015-03-09 2020-04-20 多能性幹細胞を所望の細胞型へ分化する方法
JP2021099810A Active JP7403841B2 (ja) 2015-03-09 2021-06-16 多能性幹細胞を所望の細胞型へ分化する方法
JP2023063437A Pending JP2023085519A (ja) 2015-03-09 2023-04-10 多能性幹細胞を所望の細胞型へ分化する方法
JP2024210817A Pending JP2025032187A (ja) 2015-03-09 2024-12-04 多能性幹細胞を所望の細胞型へ分化する方法

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2020075056A Active JP6903355B2 (ja) 2015-03-09 2020-04-20 多能性幹細胞を所望の細胞型へ分化する方法
JP2021099810A Active JP7403841B2 (ja) 2015-03-09 2021-06-16 多能性幹細胞を所望の細胞型へ分化する方法
JP2023063437A Pending JP2023085519A (ja) 2015-03-09 2023-04-10 多能性幹細胞を所望の細胞型へ分化する方法
JP2024210817A Pending JP2025032187A (ja) 2015-03-09 2024-12-04 多能性幹細胞を所望の細胞型へ分化する方法

Country Status (3)

Country Link
US (3) US10836997B2 (cg-RX-API-DMAC7.html)
JP (5) JP6773268B2 (cg-RX-API-DMAC7.html)
WO (1) WO2016143826A1 (cg-RX-API-DMAC7.html)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016143826A1 (ja) 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
HK1252090A1 (zh) * 2015-08-31 2019-05-17 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
CA2996582A1 (en) 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
JP7189587B2 (ja) * 2015-10-30 2022-12-14 典正 三浦 メチル化関連酵素ｈａｔ１とｋａｔ８の阻害薬
EP3431587B1 (en) * 2016-03-15 2022-10-26 University of Tsukuba Method for producing cardiac precursor cell and myocardial cell from pluripotent stem cell
CN109689860A (zh) 2016-09-12 2019-04-26 哈佛学院院长及董事 转录因子控制干细胞的分化
GB201619876D0 (en) 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
JPWO2018155620A1 (ja) * 2017-02-24 2020-01-09 剛士 田邊 神経系細胞の作製方法
US11136555B2 (en) * 2017-03-01 2021-10-05 Elixirgen Scientific, Inc. Compositions and methods for differentiation of human pluripotent stem cells into desired cell types
WO2019108894A1 (en) 2017-12-01 2019-06-06 President And Fellows Of Harvard College Methods and compositions for the production of oligodendrocyte progenitor cells
WO2019195675A1 (en) 2018-04-06 2019-10-10 President And Fellows Of Harvard College Methods of identifying combinations of transcription factors
TWI887234B (zh) 2019-04-14 2025-06-21 美國公爵大學 治療杜興氏（ｄｕｃｈｅｎｎｅ）肌肉失養症之大型突變熱點之ａａｖ載體介導之刪除
WO2020223674A2 (en) * 2019-05-01 2020-11-05 Board Of Regents Of The University Of Texas System Methods and compositions for regulating adipogenesis
MX2021014456A (es) * 2019-05-27 2022-01-06 Univ Muenster Westfaelische Wilhelms Generacion rapida y deterministica de microglia a partir de celulas madre pluripotentes humanas.
MX2021014681A (es) 2019-05-31 2022-04-06 Harvard College Células progenitoras de oligodendrocitos inducidas por la caja hmg relacionada con sry 9.
CA3151336A1 (en) * 2019-08-19 2021-02-25 Duke University Compositions and methods for identifying regulators of cell type fate specification
WO2021105234A1 (en) * 2019-11-25 2021-06-03 Asgard Therapeutics Ab Compositions for reprogramming cells into dendritic cells type 2 competent for antigen presentation, methods and uses thereof
KR20230010625A (ko) 2020-03-11 2023-01-19 비트 바이오 리미티드 간 세포 생성 방법
CN113652402A (zh) * 2020-05-12 2021-11-16 再康医药科技(上海)有限公司 一种诱导胶质细胞转分化为功能性神经元的方法及其应用
CN112626023B (zh) * 2021-01-12 2023-08-04 中国人民解放军海军军医大学 脊髓olig2+神经祖细胞诱导及自我更新培养体系、诱导方法及应用
CA3217861A1 (en) * 2021-05-07 2022-11-10 Astellas Institute For Regenerative Medicine Methods of generating mature hepatocytes
WO2022239724A1 (en) 2021-05-10 2022-11-17 Okinawa Institute Of Science And Technology School Corporation A modified rhabdovirus having a reduced toxicity
JP7306746B2 (ja) * 2021-09-16 2023-07-11 株式会社ユニバーサルエンターテインメント 遊技機
JP7268911B2 (ja) * 2021-09-16 2023-05-08 株式会社ユニバーサルエンターテインメント 遊技機
JP7306745B2 (ja) * 2021-09-16 2023-07-11 株式会社ユニバーサルエンターテインメント 遊技機
WO2024091801A2 (en) * 2022-10-13 2024-05-02 President And Fellows Of Harvard College Methods and compositions for inducing cell differentiation
WO2024218715A1 (en) 2023-04-19 2024-10-24 Btl Healthcare Technologies A.S. Culture media based on protein hydrolysate and a process for preparing thereof
US12668774B2 (en) 2023-10-12 2026-06-30 Btl Healthcare Technologies A.S. Product, system and method of cell cultivation
WO2025254221A1 (ja) * 2024-06-07 2025-12-11 国立大学法人九州大学 形質転換幹細胞、神経細胞、及び、形質転換幹細胞由来の神経細胞の作製方法
WO2026023241A1 (ja) * 2024-07-26 2026-01-29 株式会社リコー 神経系細胞の製造方法及び神経系細胞誘導剤
JP7755342B1 (ja) * 2024-10-02 2025-10-16 株式会社Dioseve 卵子様細胞を製造する方法、及び卵子様細胞

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7344857B2 (en) * 1996-09-27 2008-03-18 California Institute Of Technology Neurogenin
AU2001268255A1 (en) * 2000-06-05 2001-12-17 The Burnham Institute Methods of differentiating and protecting cells by modulating the p38/mef2 pathway
JP2004016109A (ja) 2002-06-17 2004-01-22 Nagoya Industrial Science Research Inst 軟骨細胞様細胞、及びその作製方法
US7427603B2 (en) 2002-09-26 2008-09-23 The Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
JP4706208B2 (ja) * 2004-08-27 2011-06-22 株式会社アイル 造血幹細胞の製造方法
EP2543738A3 (en) * 2006-03-07 2013-04-24 The Trustees Of The University Of Pennsylvania Random RNAi libraries, methods of generating same, and screening methods utilizing same
JP5745423B2 (ja) * 2009-10-28 2015-07-08 公益財団法人ヒューマンサイエンス振興財団 幹細胞から肝細胞への分化誘導方法
CA2796251C (en) * 2010-04-13 2019-05-14 Cellular Dynamics International, Inc. Hepatocyte production by forward programming
WO2012109208A2 (en) 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming
WO2013025963A2 (en) * 2011-08-17 2013-02-21 President And Fellows Of Harvard College Conversion of somatic cells into functional spinal motor neurons, and methods and uses thereof
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
WO2013163171A1 (en) * 2012-04-24 2013-10-31 Kaufman Dan S Method for developing natural killer cells from stem cells
JP5970245B2 (ja) 2012-06-06 2016-08-17 公益財団法人ヒューマンサイエンス振興財団 幹細胞から肝細胞への分化誘導方法
CN104955942B (zh) * 2012-10-29 2018-10-02 学校法人埼玉医科大学 分化多能性干细胞的制造方法
EP2970912B1 (en) * 2013-03-13 2019-03-06 Wisconsin Alumni Research Foundation Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
SG11201507829SA (en) 2013-03-21 2015-12-30 Univ Kyoto Pluripotent stem cell for neuronal differentiation induction
WO2016143826A1 (ja) 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法

Also Published As

Publication number Publication date
JP2025032187A (ja) 2025-03-11
US10836997B2 (en) 2020-11-17
JP7403841B2 (ja) 2023-12-25
JP2023085519A (ja) 2023-06-20
US20210024887A1 (en) 2021-01-28
WO2016143826A1 (ja) 2016-09-15
US11891621B2 (en) 2024-02-06
JP2020182452A (ja) 2020-11-12
JP2021151252A (ja) 2021-09-30
US20240124836A1 (en) 2024-04-18
JPWO2016143826A1 (ja) 2018-02-15
JP6903355B2 (ja) 2021-07-14
US20180127714A1 (en) 2018-05-10

Similar Documents

Publication Publication Date Title
JP6903355B2 (ja) 多能性幹細胞を所望の細胞型へ分化する方法
Li et al. Long non-coding RNA LncKdm2b regulates cortical neuronal differentiation by cis-activating Kdm2b
US20210123016A1 (en) Regulators of human pluripotent stem cells and uses thereof
Zhou et al. Epigenetic modification cooperates with Zeb1 transcription factor to regulate Bmp4 to promote chicken PGCs formation
JP7718819B2 (ja) 成体幹細胞の拡大と誘導のインビトロでの誘発
JP2024504412A (ja) 機能性核酸分子および方法
Wang et al. RIP-Seq of EZH2 Identifies TCONS-00036665 as a Regulator of Myogenesis in Pigs
KR20220041206A (ko) 종양발생을 억제하면서 유도 만능 줄기 세포를 생성하기 위한 pcbp1의 용도
US20250188419A1 (en) Methods and compositions for stem cell differentiation
EP3957721A1 (en) Rapid production of human neuronal cultures with single cell resolution
EP2483395B1 (en) A nuclear receptor and mutant thereof and the use of the same in the reprogramming of cells
CN119497750A (zh) 产生小胶质细胞的方法和组合物
EP3196295A1 (en) Method of producing renal cells from fibroblasts
Qi et al. Self-balanced regulation by the long non-coding RNA Lockd on the cell cycle progression of cortical neural progenitor cells through counteracting cis and trans roles
Alvarez-Dominguez et al. Regulation of eukaryotic cell differentiation by long non-coding RNAs
CN112121179A (zh) 一种组合物及其在治疗脊髓性肌萎缩症中的应用
Schneider et al. Large-scale dissection suggests that ultraconserved elements are dispensable for mouse embryonic stem cell survival and fitness
Celotto Deciphering the transcriptional states of Müller glia and their progeny in the regenerating zebrafish retina
Vila Torondel Characterization of striatal development in Huntington’s Disease and its implication to the adult phenotype
Pal Studies on LncRNA Mrhl in mouse embryonic stem cells and neuronal lineage development
HK40122965A (en) Methods and compositions for producing microglia
KR101636531B1 (ko) Six 1을 포함하는 전사인자를 이용한 근육 분화능을 갖는 근육줄기세포의 제조방법
CN120796379A (zh) 一种基于h1融合蛋白的体细胞重编程方法及其应用
CN119082248A (zh) 应激颗粒诱导剂在制备脐带间充质干细胞衰老抑制剂中的应用
CN119506223A (zh) 一种星形母细胞瘤细胞模型及其构建方法和应用

Legal Events

Date Code Title Description
AA64 Notification of invalidation of claim of internal priority (with term)

Free format text: JAPANESE INTERMEDIATE CODE: A241764

Effective date: 20171116

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171201

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190304

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190304

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200225

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200420

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200903

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200915

R150 Certificate of patent or registration of utility model

Ref document number: 6773268

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250