JP6754301B2 - ドロップレットデジタルｐｃｒの測定方法および測定装置 - Google Patents

ドロップレットデジタルｐｃｒの測定方法および測定装置 Download PDF

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JP6754301B2
JP6754301B2 JP2017000772A JP2017000772A JP6754301B2 JP 6754301 B2 JP6754301 B2 JP 6754301B2 JP 2017000772 A JP2017000772 A JP 2017000772A JP 2017000772 A JP2017000772 A JP 2017000772A JP 6754301 B2 JP6754301 B2 JP 6754301B2
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droplet
dna
oil
fluorescently labeled
labeled probe
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JP2017000772A
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Japanese (ja)
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JP2018108063A (ja
JP2018108063A5 (enExample
Inventor
淳子 田中
淳子 田中
崇秀 横井
崇秀 横井
釜堀　政男
政男 釜堀
小原　賢信
賢信 小原
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Hitachi Ltd
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Hitachi Ltd
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Priority to JP2017000772A priority Critical patent/JP6754301B2/ja
Priority to US16/476,256 priority patent/US11884973B2/en
Priority to PCT/JP2017/041290 priority patent/WO2018128013A1/ja
Publication of JP2018108063A publication Critical patent/JP2018108063A/ja
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Publication of JP6754301B2 publication Critical patent/JP6754301B2/ja
Priority to US18/529,759 priority patent/US20240102088A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017000772A 2017-01-05 2017-01-05 ドロップレットデジタルｐｃｒの測定方法および測定装置 Active JP6754301B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2017000772A JP6754301B2 (ja) 2017-01-05 2017-01-05 ドロップレットデジタルｐｃｒの測定方法および測定装置
US16/476,256 US11884973B2 (en) 2017-01-05 2017-11-16 PCR measuring method and measurement device
PCT/JP2017/041290 WO2018128013A1 (ja) 2017-01-05 2017-11-16 Ｐｃｒの測定方法および測定装置
US18/529,759 US20240102088A1 (en) 2017-01-05 2023-12-05 Pcr measuring method and measurement device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2017000772A JP6754301B2 (ja) 2017-01-05 2017-01-05 ドロップレットデジタルｐｃｒの測定方法および測定装置

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JP2020107297A Division JP7016382B2 (ja) 2020-06-22 2020-06-22 ドロップレットデジタルｐｃｒの測定方法および測定装置

Publications (3)

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JP2018108063A JP2018108063A (ja) 2018-07-12
JP2018108063A5 JP2018108063A5 (enExample) 2019-03-28
JP6754301B2 true JP6754301B2 (ja) 2020-09-09

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US (2) US11884973B2 (enExample)
JP (1) JP6754301B2 (enExample)
WO (1) WO2018128013A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7066540B2 (ja) * 2018-06-14 2022-05-13 株式会社日立製作所 デジタルｐｃｒの測定方法および測定装置
JP7602238B2 (ja) 2019-03-07 2024-12-18 国立大学法人 長崎大学 Ｔｅｒｔプロモーター変異を検出するプローブセット
JP7164927B2 (ja) * 2019-03-19 2022-11-02 株式会社日立製作所 デジタルｐｃｒ計測装置
JP7245734B2 (ja) * 2019-06-26 2023-03-24 株式会社日立製作所 デジタルｐｃｒの測定方法および測定装置
CN114929894B (zh) * 2020-01-16 2025-11-11 株式会社日立高新技术 Dna检测方法以及dna检测系统
EP4353810A4 (en) 2021-06-07 2025-04-23 Hitachi High-Tech Corporation DNA DETECTION METHOD AND DNA DETECTION SYSTEM
EP4632387A1 (en) 2022-12-07 2025-10-15 Hitachi High-Tech Corporation Sample solution separation method, sample solution separation device, and sample solution separation apparatus
CN116453602B (zh) * 2023-02-17 2024-04-19 祥符实验室 一种基于ddPCR与荧光液滴分选富集的DNA数据库随机读取方法
JP2025034360A (ja) 2023-08-31 2025-03-13 株式会社日立製作所 Ｄｎａ検出方法およびｄｎａ検出キット

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP2005229839A (ja) * 2004-02-17 2005-09-02 Sapporo Breweries Ltd 乳酸菌の検出・識別方法
JP5934657B2 (ja) * 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
US20130017544A1 (en) * 2011-07-11 2013-01-17 Advanced Liquid Logic Inc High Resolution Melting Analysis on a Droplet Actuator
JP6402905B2 (ja) * 2014-08-08 2018-10-10 東洋紡株式会社 核酸増幅の非特異反応を抑制する新規の方法
EP4613872A3 (en) * 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN107532128B (zh) * 2015-04-24 2021-07-02 豪夫迈·罗氏有限公司 使用数字微流体的数字pcr系统和方法
CN108368544B (zh) * 2015-09-29 2023-06-23 生命技术公司 用于执行数字pcr的系统和方法

Also Published As

Publication number Publication date
US11884973B2 (en) 2024-01-30
JP2018108063A (ja) 2018-07-12
WO2018128013A1 (ja) 2018-07-12
US20240102088A1 (en) 2024-03-28
US20190352699A1 (en) 2019-11-21

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