JP6698708B2 - 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 - Google Patents

分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 Download PDF

Info

Publication number
JP6698708B2
JP6698708B2 JP2017564364A JP2017564364A JP6698708B2 JP 6698708 B2 JP6698708 B2 JP 6698708B2 JP 2017564364 A JP2017564364 A JP 2017564364A JP 2017564364 A JP2017564364 A JP 2017564364A JP 6698708 B2 JP6698708 B2 JP 6698708B2
Authority
JP
Japan
Prior art keywords
sequence
tag
tagged
polynucleotides
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017564364A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018522543A (ja
JP2018522543A5 (OSRAM
Inventor
アン モンガン
アン モンガン
リチャード チェン
リチャード チェン
ドゥミトル ブリンザ
ドゥミトル ブリンザ
ケリー ブラムレット
ケリー ブラムレット
Original Assignee
ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ライフ テクノロジーズ コーポレーション, ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2018522543A publication Critical patent/JP2018522543A/ja
Publication of JP2018522543A5 publication Critical patent/JP2018522543A5/ja
Application granted granted Critical
Publication of JP6698708B2 publication Critical patent/JP6698708B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
JP2017564364A 2015-06-09 2016-06-09 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 Active JP6698708B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201562172836P 2015-06-09 2015-06-09
US62/172,836 2015-06-09
US201562207177P 2015-08-19 2015-08-19
US62/207,177 2015-08-19
US201562248978P 2015-10-30 2015-10-30
US62/248,978 2015-10-30
US201662304530P 2016-03-07 2016-03-07
US62/304,530 2016-03-07
US201662310647P 2016-03-18 2016-03-18
US62/310,647 2016-03-18
US201662311276P 2016-03-21 2016-03-21
US62/311,276 2016-03-21
US201662323142P 2016-04-15 2016-04-15
US62/323,142 2016-04-15
PCT/US2016/036763 WO2016201142A1 (en) 2015-06-09 2016-06-09 Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging

Publications (3)

Publication Number Publication Date
JP2018522543A JP2018522543A (ja) 2018-08-16
JP2018522543A5 JP2018522543A5 (OSRAM) 2019-07-04
JP6698708B2 true JP6698708B2 (ja) 2020-05-27

Family

ID=56292898

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017564364A Active JP6698708B2 (ja) 2015-06-09 2016-06-09 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体

Country Status (5)

Country Link
US (3) US10344336B2 (OSRAM)
EP (2) EP3653728B1 (OSRAM)
JP (1) JP6698708B2 (OSRAM)
CN (1) CN107849600A (OSRAM)
WO (1) WO2016201142A1 (OSRAM)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170009288A1 (en) 2014-02-03 2017-01-12 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
ES2911421T3 (es) 2015-12-08 2022-05-19 Twinstrand Biosciences Inc Adaptadores, métodos y composiciones mejorados para secuenciación dúplex
KR102461735B1 (ko) 2016-09-23 2022-11-01 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
CA3038661C (en) 2016-09-27 2025-05-06 Psomagen, Inc. METHOD AND SYSTEM FOR PREPARING AND SEQUENCING CRISPR-BASED BANK
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
EP3692161A4 (en) 2016-11-11 2022-05-25 Psomagen, Inc. METHODS AND SYSTEM FOR FRAGMENT ARRANGEMENT AND SEQUENCE IDENTIFICATION
US11352662B2 (en) 2017-01-20 2022-06-07 Sequenom, Inc. Sequence adapter manufacture and use
US10892037B2 (en) 2017-05-16 2021-01-12 Life Technologies Corporation Methods for compression of molecular tagged nucleic acid sequence data
CN110997934B (zh) * 2017-05-31 2025-02-28 生捷科技控股公司 具有电子检测系统的寡核苷酸探针阵列
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
US11702654B2 (en) * 2017-06-20 2023-07-18 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
EP3682025A1 (en) * 2017-09-14 2020-07-22 H. Hoffnabb-La Roche Ag Novel method for generating circular single-stranded dna libraries
EP3684947A1 (en) 2017-09-20 2020-07-29 Life Technologies Corporation Methods for detection of fusions using compressed molecular tagged nucleic acid sequence data
WO2019152862A1 (en) * 2018-02-02 2019-08-08 Apdn (B.V.I.) Inc. Systems and methods for tracking the origin of cannabis products and cannabis derivative products
GB201804642D0 (en) * 2018-03-22 2018-05-09 Inivata Ltd Methods of labelling nucleic acids
GB201804641D0 (en) * 2018-03-22 2018-05-09 Inivata Ltd Methods of sequencing nucleic acids and error correction of sequence reads
EP3775198A4 (en) 2018-04-02 2022-01-05 Grail, Inc. METHYLATION MARKERS AND TARGETED METHYLATION PROBE PANELS
CN108486230B (zh) * 2018-05-18 2022-02-08 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的试剂盒及其制备方法
CN108959851B (zh) * 2018-06-12 2022-03-18 哈尔滨工程大学 一种Illumina高通量测序数据误差校正方法
JP7491576B2 (ja) 2018-06-12 2024-05-28 キージーン ナムローゼ フェンノートシャップ 核酸増幅法
AU2019319711B2 (en) 2018-08-08 2025-08-28 Inivata Ltd. Method of sequencing using variable replicate multiplex PCR
EP3837380A4 (en) * 2018-08-17 2022-05-11 Cellecta, Inc. MULTIPLEX PREPARATION OF BARCODE GENE-SPECIFIC DNA FRAGMENTS
CA3100035A1 (en) 2018-08-27 2020-03-05 Regeneron Pharmaceuticals, Inc. Use of raman spectroscopy in downstream purification
CN113286881A (zh) 2018-09-27 2021-08-20 格里尔公司 甲基化标记和标靶甲基化探针板
GB2588716B (en) * 2018-12-07 2023-11-01 Element Biosciences Inc Flow cell device and use thereof
DK3899037T3 (da) * 2018-12-19 2023-11-06 Illumina Inc Fremgangsmåder til forbedring af polynukleotidklyngeklonalitetsprioritet
AU2019402900A1 (en) * 2018-12-20 2021-07-15 Alveo Technologies, Inc. Isothermal amplification with electrical detection
AU2019401601A1 (en) 2018-12-20 2021-07-15 Alveo Technologies, Inc. Handheld impedance-based diagnostic test system for detecting analytes
EP3942557A1 (en) 2019-03-22 2022-01-26 Life Technologies Corporation Methods for partner agnostic gene fusion detection
EP3748013A1 (en) * 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing a nucleic acid sequence
EP3748014A1 (en) * 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing nucleic acid sequence, apparatus for analyzing nucleic acid sequence, and program for analyzing nucleic acid sequence
EP3748015A1 (en) * 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing nucleic acid sequence, apparatus for analyzing nucleic acid sequence, and program for analyzing nucleic acid sequence
EP4017620B1 (en) * 2019-08-21 2024-05-01 Life Technologies Corporation System and method for sequencing
EP4022092A1 (en) * 2019-08-30 2022-07-06 Life Technologies Corporation Compositions and methods for oncology precision assays
US20220356500A1 (en) * 2019-12-30 2022-11-10 Yuandian Biolabs Co., Ltd. Method for preparing nucleic acid sequences using enzyme
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
WO2021146168A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
CN115768558A (zh) 2020-01-13 2023-03-07 福路伦特生物科学公司 用于单细胞基因谱分析的方法和系统
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
CN111254198A (zh) * 2020-03-09 2020-06-09 苏州宏元生物科技有限公司 一种尿路上皮癌诊断和预后评估试剂盒
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111466508A (zh) * 2020-04-10 2020-07-31 四川长虹电器股份有限公司 射频解冻装置、冰箱及射频解冻方法
EP4168576A2 (en) 2020-06-18 2023-04-26 Resolve BioSciences GmbH Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
US11959074B2 (en) 2020-11-14 2024-04-16 Life Technologies Corporation System and method for automated repeat sequencing
US11427855B1 (en) 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
EP4424845A1 (en) * 2023-03-01 2024-09-04 Personal Genomics Taiwan, Inc. Method, kit and cartridge for detecting nucleic acid molecule
WO2024196764A2 (en) * 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Assay for recombinase accessible chromatin and related compositions and methods
WO2025085618A1 (en) * 2023-10-20 2025-04-24 Illumina, Inc. Methods for appending adaptors onto polynucleotides

Family Cites Families (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
CA2116215C (en) 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5273881A (en) 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US6444806B1 (en) 1996-04-30 2002-09-03 Hisamitsu Pharmaceutical Co., Inc. Conjugates and methods of forming conjugates of oligonucleotides and carbohydrates
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
DE69826697T2 (de) 1997-07-07 2006-02-16 Medical Research Council In vitro selektionsmethode
FI103809B (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
CA2342838A1 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6830884B1 (en) 1998-12-15 2004-12-14 Molecular Staging Inc. Method of amplification
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
DE60136166D1 (de) 2000-02-07 2008-11-27 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
EP1790736A3 (en) 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2002222737A1 (en) 2001-12-08 2003-06-23 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
EP1319718A1 (en) 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US20040038331A1 (en) 2002-08-23 2004-02-26 Reddy M. Parameswara Solid phase synthesis of biomolecule conjugates
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP1709203A2 (en) 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
US20080003571A1 (en) 2005-02-01 2008-01-03 Mckernan Kevin Reagents, methods, and libraries for bead-based sequencing
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
AU2006242387B2 (en) 2005-04-29 2011-01-06 Synthetic Genomics, Inc. Amplification and cloning of single DNA molecules using rolling circle amplification
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
DK2292788T3 (da) 2005-06-23 2012-07-23 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
ES2420831T3 (es) 2005-07-25 2013-08-27 Alere San Diego, Inc. Procedimientos para multiplexar la ampliación de la recombinasa polimerasa
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
JP5237099B2 (ja) 2005-09-29 2013-07-17 キージーン ナムローゼ フェンノートシャップ 変異させた集団のハイスループットスクリーニング
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
WO2008039674A2 (en) 2006-09-20 2008-04-03 Luminescent Technologies, Inc. Photo-mask and wafer image reconstruction
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2129803A4 (en) 2007-02-27 2010-11-03 Univ Northwestern APPLICATION OF MOLECULES TO NANOPARTICLES
CA2700838A1 (en) 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8271206B2 (en) * 2008-04-21 2012-09-18 Softgenetics Llc DNA sequence assembly methods of short reads
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
GB2461127B (en) 2008-06-25 2010-07-14 Ion Torrent Systems Inc Methods and apparatus for measuring analytes using large scale FET arrays
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8546128B2 (en) 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9394567B2 (en) * 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
SG171760A1 (en) 2008-11-19 2011-07-28 Amyris Inc Compositions and methods for the assembly of polynucleotides
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
ES3022638T3 (en) 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US20120045368A1 (en) 2010-08-18 2012-02-23 Life Technologies Corporation Chemical Coating of Microwell for Electrochemical Detection Device
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
WO2012058459A2 (en) 2010-10-27 2012-05-03 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
CN103370425B (zh) 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドＦｏｕｎｄａｔｉｏｎ Ｍｅｄｉｃｉｎｅ， Ｉｎｃ． 腫瘍試料の多重遺伝子分析の最適化
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US20130090860A1 (en) 2010-12-30 2013-04-11 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
US9617296B2 (en) 2011-04-08 2017-04-11 Life Technologies Corporation Methods and kits for breaking emulsions
CN110016499B (zh) * 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
US9809904B2 (en) * 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
EP3260557A1 (en) 2011-05-27 2017-12-27 Life Technologies Corporation Methods for manipulating biomolecules
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
WO2013130512A2 (en) * 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
GB2513024B (en) * 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US9862995B2 (en) * 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
WO2013142389A1 (en) * 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US20150126376A1 (en) 2012-06-14 2015-05-07 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20160115532A1 (en) 2012-08-10 2016-04-28 Sequenta, Inc. High sensitivity mutation detection using sequence tags
ES2769241T5 (es) 2012-09-04 2023-05-30 Guardant Health Inc Sistemas y métodos para detectar variación en el número de copias
US20140066317A1 (en) * 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN103045726B (zh) * 2012-11-20 2015-11-25 南方科技大学 对多个混合dna或rna序列进行基因测序的方法及装置
SG10202009015SA (en) 2012-12-10 2020-10-29 Resolution Bioscience Inc Methods for targeted genomic analysis
US20160186267A1 (en) * 2013-02-21 2016-06-30 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
US20140255929A1 (en) * 2013-03-11 2014-09-11 Sequenta, Inc. Mosaic tags for labeling templates in large-scale amplifications
EP3249057B1 (en) 2013-03-14 2019-05-01 Life Technologies Corporation Matrix arrays and methods for making same
ITMI20130496A1 (it) 2013-03-29 2014-09-30 Atlas Copco Blm Srl Gruppo e disposizione di montaggio di una spina di un connettore circolare a baionetta su un pannello o una parete, in particolare un pannello o una parete di un corpo scatolare di contenimento di un dispositivo elettrico o elettronico
US20140357499A1 (en) * 2013-05-30 2014-12-04 Washington University METHODS OF LOW ERROR AMPLICON SEQUENCING (LEA-Seq) AND THE USE THEREOF
WO2015031559A1 (en) 2013-08-30 2015-03-05 Tensor Surgical, Inc Passive retrieving interosseous suture passing device
GB2518668B (en) 2013-09-28 2017-07-12 Design Blue Ltd Flexible pads and shield systems
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
WO2015108663A1 (en) 2014-01-16 2015-07-23 Illumina, Inc. Amplicon preparation and sequencing on solid supports
US20170009288A1 (en) 2014-02-03 2017-01-12 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
CN106687805B (zh) 2014-07-02 2020-05-29 生命技术公司 半导体传感器的表面处理
US12398391B2 (en) 2014-07-15 2025-08-26 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体

Also Published As

Publication number Publication date
US20190323091A1 (en) 2019-10-24
EP3653728B1 (en) 2023-02-01
US11124842B2 (en) 2021-09-21
US20160362748A1 (en) 2016-12-15
JP2018522543A (ja) 2018-08-16
US20210363596A1 (en) 2021-11-25
EP3653728A1 (en) 2020-05-20
US12338496B2 (en) 2025-06-24
WO2016201142A1 (en) 2016-12-15
US10344336B2 (en) 2019-07-09
EP3307908B1 (en) 2019-09-11
CN107849600A (zh) 2018-03-27
EP3307908A1 (en) 2018-04-18

Similar Documents

Publication Publication Date Title
JP6698708B2 (ja) 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
US11725241B2 (en) Compositions and methods for identification of a duplicate sequencing read
EP3377625B1 (en) Method for controlled dna fragmentation
US10072283B2 (en) Direct capture, amplification and sequencing of target DNA using immobilized primers
KR102709499B1 (ko) 단일 세포 전체 게놈 라이브러리 및 이의 제조를 위한 조합 인덱싱 방법
CN109689888B (zh) 无细胞核酸标准品及其用途
EP3715468A1 (en) Methods and compositions for preparing sequencing libraries
EP4180539A1 (en) Single end duplex dna sequencing
US11136576B2 (en) Method for controlled DNA fragmentation
EP4267763B1 (en) Compositions and methods for highly sensitive detection of target sequences in multiplex reactions
CN114450420A (zh) 用于肿瘤学精确测定的组合物和方法
HK40027672B (zh) 用於甲基化测序的单细胞全基因组文库

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171225

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190603

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190603

TRDD Decision of grant or rejection written
A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20200325

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200330

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200428

R150 Certificate of patent or registration of utility model

Ref document number: 6698708

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250