JP6698696B2 - 核酸分子の検出 - Google Patents

核酸分子の検出 Download PDF

Info

Publication number
JP6698696B2
JP6698696B2 JP2017557123A JP2017557123A JP6698696B2 JP 6698696 B2 JP6698696 B2 JP 6698696B2 JP 2017557123 A JP2017557123 A JP 2017557123A JP 2017557123 A JP2017557123 A JP 2017557123A JP 6698696 B2 JP6698696 B2 JP 6698696B2
Authority
JP
Japan
Prior art keywords
nucleic acid
biological sample
acid molecule
pcr
particular nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017557123A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018514218A5 (enExample
JP2018514218A (ja
Inventor
シュピーケルマン,マイケ
ヴィンター,ニーナ
フロール,インガ
ベルジュ,ガザンフェル
Original Assignee
ミーアディテクト ゲーエムベーハー
ミーアディテクト ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ミーアディテクト ゲーエムベーハー, ミーアディテクト ゲーエムベーハー filed Critical ミーアディテクト ゲーエムベーハー
Publication of JP2018514218A publication Critical patent/JP2018514218A/ja
Publication of JP2018514218A5 publication Critical patent/JP2018514218A5/ja
Application granted granted Critical
Publication of JP6698696B2 publication Critical patent/JP6698696B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017557123A 2015-04-29 2016-04-29 核酸分子の検出 Active JP6698696B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102015106646 2015-04-29
DE102015106646.7 2015-04-29
EP15195182 2015-11-18
EP15195182.9 2015-11-18
PCT/EP2016/059604 WO2016174199A1 (en) 2015-04-29 2016-04-29 Detection of nucleic acid molecules

Publications (3)

Publication Number Publication Date
JP2018514218A JP2018514218A (ja) 2018-06-07
JP2018514218A5 JP2018514218A5 (enExample) 2019-05-09
JP6698696B2 true JP6698696B2 (ja) 2020-05-27

Family

ID=55862785

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017557123A Active JP6698696B2 (ja) 2015-04-29 2016-04-29 核酸分子の検出

Country Status (17)

Country Link
US (1) US10889855B2 (enExample)
EP (1) EP3289095B1 (enExample)
JP (1) JP6698696B2 (enExample)
KR (1) KR102478976B1 (enExample)
CN (1) CN107873058B (enExample)
AU (1) AU2016256581B2 (enExample)
CA (1) CA2982416A1 (enExample)
DK (1) DK3289095T5 (enExample)
EA (1) EA037535B1 (enExample)
ES (1) ES2736850T3 (enExample)
HU (1) HUE045865T2 (enExample)
IL (1) IL255081B (enExample)
MX (1) MX369054B (enExample)
PL (1) PL3289095T3 (enExample)
PT (1) PT3289095T (enExample)
SG (1) SG11201708274VA (enExample)
WO (1) WO2016174199A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7391367B2 (ja) 2016-11-02 2023-12-05 ミーアディテクト ゲーエムベーハー 前段階の精巣胚細胞腫瘍の早期発見
US12065644B2 (en) * 2017-02-16 2024-08-20 Takara Bio Usa, Inc. Methods of preparing nucleic acid libraries and compositions and kits for practicing the same
CN111363840A (zh) * 2020-03-30 2020-07-03 上海市计量测试技术研究院 一种检测基于RNAi转基因植物双链RNA的试剂盒及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101363057B (zh) * 2008-09-09 2011-11-09 浙江理工大学 一种生物样本中miRNA绝对表达量的检测方法

Also Published As

Publication number Publication date
DK3289095T5 (da) 2019-07-29
HUE045865T2 (hu) 2020-01-28
IL255081A0 (en) 2017-12-31
EP3289095B1 (en) 2019-07-03
MX2017013454A (es) 2018-03-15
MX369054B (es) 2019-10-28
US20180163264A1 (en) 2018-06-14
EP3289095A1 (en) 2018-03-07
EA037535B1 (ru) 2021-04-09
PL3289095T3 (pl) 2020-01-31
EA201791987A1 (ru) 2018-05-31
KR102478976B1 (ko) 2022-12-19
WO2016174199A1 (en) 2016-11-03
US10889855B2 (en) 2021-01-12
JP2018514218A (ja) 2018-06-07
KR20180002666A (ko) 2018-01-08
NZ736174A (en) 2024-10-25
CN107873058A (zh) 2018-04-03
SG11201708274VA (en) 2017-11-29
CA2982416A1 (en) 2016-11-03
IL255081B (en) 2020-10-29
AU2016256581A1 (en) 2017-10-26
DK3289095T3 (da) 2019-07-22
PT3289095T (pt) 2019-07-25
CN107873058B (zh) 2022-08-02
AU2016256581B2 (en) 2021-08-19
ES2736850T3 (es) 2020-01-08

Similar Documents

Publication Publication Date Title
CN102154505B (zh) 一种检测miRNA的方法及引物及其应用
KR101987358B1 (ko) 신규한 간암 진단용 바이오 마커 및 이의 용도
CN103834719A (zh) miRNA检测探针及miRNA的扩增检测方法
Zendjabil Preanalytical, analytical and postanalytical considerations in circulating microRNAs measurement
JP6698696B2 (ja) 核酸分子の検出
CN107299129B (zh) 循环核酸作为乳腺癌生物标志物的应用
JP2022088555A (ja) 前段階の精巣胚細胞腫瘍の早期発見
CN103555848B (zh) 小RNA的3’-5’-qPCR定量检测技术
US20150247202A1 (en) Microrna based method for diagnosis of colorectal tumors and of metastasis
CN104120174A (zh) 多个microRNAs复合检测的引物设计方法
HK1251262B (en) Detection of nucleic acid molecules
HK1251262A1 (en) Detection of nucleic acid molecules
HK40013273A (en) Early detection of preliminary stages of testicular germ cell tumors
HK40013273B (en) Early detection of preliminary stages of testicular germ cell tumors
Jäck et al. MicroRNAs and Biomarker Discovery
BR112019005790B1 (pt) Método para detectar rnas pequenos ou proteínas associadas a rnas pequenos

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190319

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190319

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20191218

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200107

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200317

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200331

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200428

R150 Certificate of patent or registration of utility model

Ref document number: 6698696

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250