KR102478976B1 - 핵산 분자의 검출 - Google Patents

핵산 분자의 검출 Download PDF

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KR102478976B1
KR102478976B1 KR1020177032038A KR20177032038A KR102478976B1 KR 102478976 B1 KR102478976 B1 KR 102478976B1 KR 1020177032038 A KR1020177032038 A KR 1020177032038A KR 20177032038 A KR20177032038 A KR 20177032038A KR 102478976 B1 KR102478976 B1 KR 102478976B1
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mir
nucleic acid
acid molecule
biological sample
pcr
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KR20180002666A (ko
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메이케 스피커만
니나 윈터
잉가 플로르
가잔페르 벨제
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미르데텍트 게엠베하
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
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    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020177032038A 2015-04-29 2016-04-29 핵산 분자의 검출 Active KR102478976B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102015106646 2015-04-29
DE102015106646.7 2015-04-29
EP15195182 2015-11-18
EP15195182.9 2015-11-18
PCT/EP2016/059604 WO2016174199A1 (en) 2015-04-29 2016-04-29 Detection of nucleic acid molecules

Publications (2)

Publication Number Publication Date
KR20180002666A KR20180002666A (ko) 2018-01-08
KR102478976B1 true KR102478976B1 (ko) 2022-12-19

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US (1) US10889855B2 (enExample)
EP (1) EP3289095B1 (enExample)
JP (1) JP6698696B2 (enExample)
KR (1) KR102478976B1 (enExample)
CN (1) CN107873058B (enExample)
AU (1) AU2016256581B2 (enExample)
CA (1) CA2982416A1 (enExample)
DK (1) DK3289095T5 (enExample)
EA (1) EA037535B1 (enExample)
ES (1) ES2736850T3 (enExample)
HU (1) HUE045865T2 (enExample)
IL (1) IL255081B (enExample)
MX (1) MX369054B (enExample)
PL (1) PL3289095T3 (enExample)
PT (1) PT3289095T (enExample)
SG (1) SG11201708274VA (enExample)
WO (1) WO2016174199A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7391367B2 (ja) 2016-11-02 2023-12-05 ミーアディテクト ゲーエムベーハー 前段階の精巣胚細胞腫瘍の早期発見
US12065644B2 (en) * 2017-02-16 2024-08-20 Takara Bio Usa, Inc. Methods of preparing nucleic acid libraries and compositions and kits for practicing the same
CN111363840A (zh) * 2020-03-30 2020-07-03 上海市计量测试技术研究院 一种检测基于RNAi转基因植物双链RNA的试剂盒及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101363057B (zh) * 2008-09-09 2011-11-09 浙江理工大学 一种生物样本中miRNA绝对表达量的检测方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Data Analysis Guide, EXIQON, for the miRCURY LNA Universal RT microRNA ready-to-use PCR panels using Exiqon GenEx software version 2.5, 2013.
Karlheinz Semmelmann et al., Qiagen Inc, Scientific article, 2013.
M. Spiekermann et al., Andrology, 2015, 3:78-84.
Yongxin Chen et al., BMC Genomics, 2009, 10:407.

Also Published As

Publication number Publication date
DK3289095T5 (da) 2019-07-29
HUE045865T2 (hu) 2020-01-28
IL255081A0 (en) 2017-12-31
EP3289095B1 (en) 2019-07-03
MX2017013454A (es) 2018-03-15
MX369054B (es) 2019-10-28
US20180163264A1 (en) 2018-06-14
EP3289095A1 (en) 2018-03-07
EA037535B1 (ru) 2021-04-09
JP6698696B2 (ja) 2020-05-27
PL3289095T3 (pl) 2020-01-31
EA201791987A1 (ru) 2018-05-31
WO2016174199A1 (en) 2016-11-03
US10889855B2 (en) 2021-01-12
JP2018514218A (ja) 2018-06-07
KR20180002666A (ko) 2018-01-08
NZ736174A (en) 2024-10-25
CN107873058A (zh) 2018-04-03
SG11201708274VA (en) 2017-11-29
CA2982416A1 (en) 2016-11-03
IL255081B (en) 2020-10-29
AU2016256581A1 (en) 2017-10-26
DK3289095T3 (da) 2019-07-22
PT3289095T (pt) 2019-07-25
CN107873058B (zh) 2022-08-02
AU2016256581B2 (en) 2021-08-19
ES2736850T3 (es) 2020-01-08

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