JP6626848B2 - 単一の管を付加したプロトコルを用いるタグ化核酸ライブラリの調製 - Google Patents

単一の管を付加したプロトコルを用いるタグ化核酸ライブラリの調製 Download PDF

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JP6626848B2
JP6626848B2 JP2016574392A JP2016574392A JP6626848B2 JP 6626848 B2 JP6626848 B2 JP 6626848B2 JP 2016574392 A JP2016574392 A JP 2016574392A JP 2016574392 A JP2016574392 A JP 2016574392A JP 6626848 B2 JP6626848 B2 JP 6626848B2
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JP2017519774A (ja
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ケイパー フィオナ
ケイパー フィオナ
キャン ゴルドン
キャン ゴルドン
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イラミーナ インコーポレーテッド
イラミーナ インコーポレーテッド
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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JP2016574392A 2014-06-26 2015-06-25 単一の管を付加したプロトコルを用いるタグ化核酸ライブラリの調製 Active JP6626848B2 (ja)

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US201462017786P 2014-06-26 2014-06-26
US62/017,786 2014-06-26
US201462027198P 2014-07-21 2014-07-21
US62/027,198 2014-07-21
PCT/US2015/037653 WO2015200609A1 (en) 2014-06-26 2015-06-25 Library preparation of tagged nucleic acid using single tube add-on protocol

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US11085041B2 (en) 2021-08-10
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