JP6616822B2 - バクテリオファージ・ラムダ・インテグラーゼの変異体 - Google Patents

バクテリオファージ・ラムダ・インテグラーゼの変異体 Download PDF

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JP6616822B2
JP6616822B2 JP2017506963A JP2017506963A JP6616822B2 JP 6616822 B2 JP6616822 B2 JP 6616822B2 JP 2017506963 A JP2017506963 A JP 2017506963A JP 2017506963 A JP2017506963 A JP 2017506963A JP 6616822 B2 JP6616822 B2 JP 6616822B2
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nucleic acid
sequence
seq
lambda integrase
amino acid
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JP2017525355A5 (enExample
JP2017525355A (ja
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ガデッシー、ファリッド
シャウ、ジャ・ウェイ
ドローゲ、ピーター
マキジャ、ハルシャ
ビジャヤ・チャンドラ、シュリー・ハルシャ
ピーター、サブリナ
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エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2017506963A 2014-08-08 2015-08-11 バクテリオファージ・ラムダ・インテグラーゼの変異体 Active JP6616822B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1414130.3A GB201414130D0 (en) 2014-08-08 2014-08-08 Mutants of the bacteriophage lambda integrase
GB1414130.3 2014-08-08
PCT/SG2015/050255 WO2016022075A1 (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

Publications (3)

Publication Number Publication Date
JP2017525355A JP2017525355A (ja) 2017-09-07
JP2017525355A5 JP2017525355A5 (enExample) 2018-09-13
JP6616822B2 true JP6616822B2 (ja) 2019-12-04

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JP2017506963A Active JP6616822B2 (ja) 2014-08-08 2015-08-11 バクテリオファージ・ラムダ・インテグラーゼの変異体

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Country Link
US (2) US10344301B2 (enExample)
EP (1) EP3177717A4 (enExample)
JP (1) JP6616822B2 (enExample)
CN (1) CN108064279B (enExample)
GB (1) GB201414130D0 (enExample)
SG (2) SG11201700955SA (enExample)
WO (1) WO2016022075A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
PL3638776T3 (pl) 2017-06-14 2026-02-23 Technische Universität Dresden Sposoby i środki do zmiany genetycznej genomów z wykorzystaniem projektowanych enzymów rekombinujących dna
US12566178B2 (en) 2019-07-29 2026-03-03 Larimar Therapeutics, Inc. Methods of quantifying frataxin and frataxin fusion proteins
DK4004022T3 (da) * 2019-07-29 2026-04-07 Univ Indiana Trustees Materialer og fremgangsmåder til behandling af friedreichs ataksi
US11988675B2 (en) 2019-11-25 2024-05-21 Larimar Therapeutics, Inc. Methods for quantifying frataxin activity
BR112022021823A2 (pt) 2020-04-30 2023-01-17 Larimar Therapeutics Inc Métodos para tratar doenças associadas à mielina e doenças associadas à mitocôndria
US12390509B2 (en) 2020-12-12 2025-08-19 Larimar Therapeutics, Inc. Pharmaceutical composition comprising frataxin fusion protein and methods of use thereof
CN117178056A (zh) * 2021-03-12 2023-12-05 南洋理工大学 用于生产无缝dna载体的方法
CN119317717A (zh) * 2022-05-31 2025-01-14 南洋理工大学 产生用于λ整合酶介导的重组的真核宿主细胞或细胞系的方法
CN118546903A (zh) * 2023-02-24 2024-08-27 尧唐(上海)生物科技有限公司 能介导大片段dna整合到哺乳动物基因组的酶及其应用
WO2024181932A1 (en) * 2023-03-02 2024-09-06 Nanyang Technological University Lambda integrase mutants and methods of their use
KR20260044268A (ko) 2023-06-26 2026-04-01 유니버시티 오브 하와이 진화한 인테그라제 및 이를 이용한 게놈 편집 방법
CN117417916A (zh) * 2023-10-25 2024-01-19 南方科技大学 酪氨酸整合酶、核酸分子、载体、宿主细胞、应用、遗传操作工具

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4489424B2 (ja) * 2001-05-30 2010-06-23 グラクソ グループ リミテッド 染色体に基くプラットホーム
CA2522166C (en) * 2001-05-30 2011-05-03 Chromos Molecular Systems, Inc. Lambda integrase mutein for use in recombination
CN103396904B (zh) * 2008-11-11 2015-12-23 宝洁公司 包含丝氨酸蛋白酶变体的组合物和方法
SG170652A1 (en) * 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase

Also Published As

Publication number Publication date
US11414681B2 (en) 2022-08-16
US20170327847A1 (en) 2017-11-16
WO2016022075A1 (en) 2016-02-11
US10344301B2 (en) 2019-07-09
CN108064279A (zh) 2018-05-22
EP3177717A4 (en) 2017-12-27
EP3177717A1 (en) 2017-06-14
SG10202005089XA (en) 2020-07-29
SG11201700955SA (en) 2017-03-30
GB201414130D0 (en) 2014-09-24
JP2017525355A (ja) 2017-09-07
CN108064279B (zh) 2022-03-11
US20200063165A1 (en) 2020-02-27

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