CN108064279B - 噬菌体λ整合酶的突变体 - Google Patents

噬菌体λ整合酶的突变体 Download PDF

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CN108064279B
CN108064279B CN201580054345.8A CN201580054345A CN108064279B CN 108064279 B CN108064279 B CN 108064279B CN 201580054345 A CN201580054345 A CN 201580054345A CN 108064279 B CN108064279 B CN 108064279B
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nucleic acid
sequence
integrase
seq
recombination
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CN108064279A (zh
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F·葛达西
箫嘉玮
P·德罗格
H·玛坎加
S·H·维贾雅钱德拉
S·彼得
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Agency for Science Technology and Research Singapore
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201580054345.8A 2014-08-08 2015-08-11 噬菌体λ整合酶的突变体 Active CN108064279B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1414130.3 2014-08-08
GBGB1414130.3A GB201414130D0 (en) 2014-08-08 2014-08-08 Mutants of the bacteriophage lambda integrase
PCT/SG2015/050255 WO2016022075A1 (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

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CN108064279A CN108064279A (zh) 2018-05-22
CN108064279B true CN108064279B (zh) 2022-03-11

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US (2) US10344301B2 (enExample)
EP (1) EP3177717A4 (enExample)
JP (1) JP6616822B2 (enExample)
CN (1) CN108064279B (enExample)
GB (1) GB201414130D0 (enExample)
SG (2) SG10202005089XA (enExample)
WO (1) WO2016022075A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
CA3149188A1 (en) * 2019-07-29 2021-02-04 The Trustees Of Indiana University Materials and methods for treating friedreich's ataxia
CA3162628A1 (en) 2019-11-25 2021-06-03 Larimar Therapeutics, Inc. Methods for quantifying frataxin activity
KR20230005333A (ko) 2020-04-30 2023-01-09 라리마 테라퓨틱스, 인코포레이티드 미엘린 관련 질환 및 미토콘드리아 관련 질환을 치료하기 위한 방법
US12390509B2 (en) 2020-12-12 2025-08-19 Larimar Therapeutics, Inc. Pharmaceutical composition comprising frataxin fusion protein and methods of use thereof
WO2022191779A1 (en) * 2021-03-12 2022-09-15 Nanyang Technological University Method for the production of seamless dna vectors
CN119317717A (zh) * 2022-05-31 2025-01-14 南洋理工大学 产生用于λ整合酶介导的重组的真核宿主细胞或细胞系的方法
CN118546903A (zh) * 2023-02-24 2024-08-27 尧唐(上海)生物科技有限公司 能介导大片段dna整合到哺乳动物基因组的酶及其应用
WO2024181932A1 (en) * 2023-03-02 2024-09-06 Nanyang Technological University Lambda integrase mutants and methods of their use
WO2025006468A2 (en) 2023-06-26 2025-01-02 University Of Hawaii Evolved integrases and methods of using the same for genome editing

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097059A2 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
CA2522166C (en) * 2001-05-30 2011-05-03 Chromos Molecular Systems, Inc. Lambda integrase mutein for use in recombination
BR122013014156A2 (pt) * 2008-11-11 2015-07-14 Danisco Us Inc Composição de limpeza compreendendo variantes de subtilisina, bem como processo de limpeza
SG170652A1 (en) 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097059A2 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Identifying Determinants of Recombination Specificity: Construction and Characterization of Chimeric Bacteriophage Integrases;Ezra Yagi et al.,;《Journal of Molecular Biology》;19950915;第252卷(第2期);163-177 *
Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization;Yvonne Tay et al.,;《Nucleic Acids Research》;20100301;第38卷(第4期);e25 *

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US11414681B2 (en) 2022-08-16
JP6616822B2 (ja) 2019-12-04
WO2016022075A1 (en) 2016-02-11
US20170327847A1 (en) 2017-11-16
JP2017525355A (ja) 2017-09-07
SG10202005089XA (en) 2020-07-29
EP3177717A1 (en) 2017-06-14
CN108064279A (zh) 2018-05-22
SG11201700955SA (en) 2017-03-30
GB201414130D0 (en) 2014-09-24
US10344301B2 (en) 2019-07-09
US20200063165A1 (en) 2020-02-27
EP3177717A4 (en) 2017-12-27

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