JP6542676B2 - ナノフルイディクスにおける分子の特性解析 - Google Patents

ナノフルイディクスにおける分子の特性解析 Download PDF

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JP6542676B2
JP6542676B2 JP2015558929A JP2015558929A JP6542676B2 JP 6542676 B2 JP6542676 B2 JP 6542676B2 JP 2015558929 A JP2015558929 A JP 2015558929A JP 2015558929 A JP2015558929 A JP 2015558929A JP 6542676 B2 JP6542676 B2 JP 6542676B2
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nucleic acid
molecule
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JP2016510590A5 (https=
JP2016510590A (ja
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ハン カオ、
ハン カオ、
アレックス、 アール． ハスティー、
アレックス、 アール． ハスティー、
アーネスト、 チ‐ツン ラム、
アーネスト、 チ‐ツン ラム、
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バイオナノ ジェノミクス、 インコーポレイテッド
バイオナノ ジェノミクス、 インコーポレイテッド
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015558929A 2013-02-20 2014-02-19 ナノフルイディクスにおける分子の特性解析 Active JP6542676B2 (ja)

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JP2019110164A JP6928032B2 (ja) 2013-02-20 2019-06-13 ナノフルイディクスにおける分子の特性解析

Applications Claiming Priority (3)

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US201361767219P 2013-02-20 2013-02-20
US61/767,219 2013-02-20
PCT/US2014/017226 WO2014130589A1 (en) 2013-02-20 2014-02-19 Characterization of molecules in nanofluidics

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JP2016510590A5 JP2016510590A5 (https=) 2017-03-16
JP6542676B2 true JP6542676B2 (ja) 2019-07-10

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US (4) US9809855B2 (https=)
EP (1) EP2959015B1 (https=)
JP (2) JP6542676B2 (https=)
CN (1) CN105229168B (https=)
AU (1) AU2014219001A1 (https=)
CA (1) CA2901460A1 (https=)
WO (1) WO2014130589A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2959015B1 (en) 2013-02-20 2020-11-04 Bionano Genomics, Inc. Characterization of molecules in nanofluidics
US10844424B2 (en) 2013-02-20 2020-11-24 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
CN106164295B (zh) 2014-02-25 2020-08-11 生物纳米基因公司 减小基因组覆盖测量中的偏差
WO2018148903A1 (zh) * 2017-02-16 2018-08-23 上海亿康医学检验所有限公司 泌尿系统肿瘤的辅助诊断方法
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
CA3177420A1 (en) * 2020-12-15 2022-06-23 Quantum-Si Incorporated Ultrasensitive biosensor methods
EP4639177A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Instrumentation of optical genome mapping systems
WO2024145268A2 (en) 2022-12-25 2024-07-04 Bionano Genomics, Inc. Optical genome mapping cartridges
EP4639178A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Optical genome mapping system

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
CA2454570C (en) 2001-07-25 2016-12-20 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
KR101086611B1 (ko) * 2002-06-20 2011-11-23 프리메라디엑스, 인크. 폴리뉴클레오티드 감지와 정량을 위한 기구
US8346482B2 (en) * 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
US20100216153A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060063184A1 (en) 2004-09-09 2006-03-23 Felix Carolyn A Compositions and methods for the detection of DNA topoisomerase II complexes with DNA
JP2009529887A (ja) * 2006-03-13 2009-08-27 イコニシス インコーポレーテッド 胎児細胞およびガン細胞を診断する非侵襲的方法
EP2049262A2 (en) 2006-07-19 2009-04-22 Bionanomatrix, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
CA2682275C (en) 2007-03-28 2017-05-09 Bionanomatrix, Inc. Methods of macromolecular analysis using nanochannel arrays
LT2557517T (lt) * 2007-07-23 2023-01-10 The Chinese University Of Hong Kong Nukleino rūgščių sekos disbalanso nustatymas
EP2194847A1 (en) 2007-09-14 2010-06-16 Corventis, Inc. Adherent device with multiple physiological sensors
EP2211142A4 (en) 2007-11-12 2017-01-25 Murata Manufacturing Co. Ltd. Operating position detector and apparatus to which the same is applied
CA3060930C (en) 2008-06-06 2022-03-22 Bionanomatrix, Inc. Integrated analysis devices and related fabrication methods and analysis techniques
KR20110025993A (ko) * 2008-06-30 2011-03-14 바이오나노매트릭스, 인크. 단일-분자 전체 게놈 분석용 장치 및 방법
EP2370594B1 (en) 2008-11-18 2014-01-08 BioNano Genomics, Inc. Polynucleotide mapping and sequencing
US20100137154A1 (en) * 2008-12-01 2010-06-03 Ach Robert A Genome analysis using a methyltransferase
EP2881701A1 (en) 2009-05-19 2015-06-10 Bionano Genomics, Inc. Devices and methods for dynamic determination of sample spatial orientation and dynamic repositioning
US9725315B2 (en) 2009-09-28 2017-08-08 Bionano Genomics, Inc. Nanochannel arrays and near-field illumination devices for polymer analysis and related methods
US20110117577A1 (en) 2009-10-20 2011-05-19 Agency For Science, Technology And Research Microfluidic system for trapping and detection of a biological entity in a sample
AU2010310638A1 (en) 2009-10-21 2012-05-03 Bionano Genomics, Inc. Methods and related devices for single molecule whole genome analysis
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US9458489B2 (en) * 2010-03-04 2016-10-04 Massachusetts Institute Of Technology Microfluidics sorter for cell detection and isolation
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
IT1399366B1 (it) 2010-04-08 2013-04-16 Weightpack S P A Camma variabile, particolarmente per macchine per l'applicazione di tappi.
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
CN106048000B (zh) 2010-10-20 2020-05-01 生物纳米基因公司 用于评估生物分子特性的系统和方法
AU2011358564B9 (en) 2011-02-09 2017-07-13 Natera, Inc Methods for non-invasive prenatal ploidy calling
DK2561103T3 (da) * 2011-06-29 2014-10-20 Bgi Diagnosis Co Ltd Ikke-invasiv påvisning af føtal genetisk anomali
WO2013036860A1 (en) 2011-09-08 2013-03-14 Bionano Genomics, Inc. Physical map construction of whole genome and pooled clone mapping in nanochannel array
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2886508T3 (es) 2011-10-06 2021-12-20 Sequenom Inc Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3243908B1 (en) 2011-10-11 2019-01-02 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013109981A1 (en) 2012-01-20 2013-07-25 Sequenom, Inc. Diagnostic processes that factor experimental conditions
WO2013166517A1 (en) 2012-05-04 2013-11-07 Complete Genomics, Inc. Methods for determining absolute genome-wide copy number variations of complex tumors
DK3663409T3 (da) 2012-05-21 2021-12-13 Sequenom Inc Fremgangsmåder og processer til ikke-invasiv bedømmelse af genetiske variationer
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9347127B2 (en) 2012-07-16 2016-05-24 Veeco Instruments, Inc. Film deposition assisted by angular selective etch on a surface
CA2878246C (en) 2012-07-20 2022-01-11 Verinata Health, Inc. Detecting and classifying copy number variation in a cancer genome
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US12385089B2 (en) 2013-02-05 2025-08-12 Bionano Genomics, Inc. Methods for single-molecule analysis
EP2959015B1 (en) 2013-02-20 2020-11-04 Bionano Genomics, Inc. Characterization of molecules in nanofluidics

Also Published As

Publication number Publication date
EP2959015A4 (en) 2016-08-17
EP2959015A1 (en) 2015-12-30
US20180223361A1 (en) 2018-08-09
US11359244B2 (en) 2022-06-14
WO2014130589A1 (en) 2014-08-28
JP2019205434A (ja) 2019-12-05
US20200362413A1 (en) 2020-11-19
US10669586B2 (en) 2020-06-02
JP6928032B2 (ja) 2021-09-01
HK1219511A1 (zh) 2017-04-07
JP2016510590A (ja) 2016-04-11
EP2959015B1 (en) 2020-11-04
US20220340973A1 (en) 2022-10-27
US9809855B2 (en) 2017-11-07
CN105229168B (zh) 2020-07-17
CN105229168A (zh) 2016-01-06
CA2901460A1 (en) 2014-08-28
AU2014219001A1 (en) 2015-10-08
US20160046992A1 (en) 2016-02-18
US12275992B2 (en) 2025-04-15

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