CA2901460A1 - Characterization of molecules in nanofluidics - Google Patents

Characterization of molecules in nanofluidics Download PDF

Info

Publication number
CA2901460A1
CA2901460A1 CA2901460A CA2901460A CA2901460A1 CA 2901460 A1 CA2901460 A1 CA 2901460A1 CA 2901460 A CA2901460 A CA 2901460A CA 2901460 A CA2901460 A CA 2901460A CA 2901460 A1 CA2901460 A1 CA 2901460A1
Authority
CA
Canada
Prior art keywords
sample
label
molecules
molecule
labeling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2901460A
Other languages
English (en)
French (fr)
Inventor
Han Cao
Alex R. Hastie
Ernest Tsz-Tsun Lam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bionano Genomics Inc
Original Assignee
Bionano Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionano Genomics Inc filed Critical Bionano Genomics Inc
Publication of CA2901460A1 publication Critical patent/CA2901460A1/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physiology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2901460A 2013-02-20 2014-02-19 Characterization of molecules in nanofluidics Pending CA2901460A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361767219P 2013-02-20 2013-02-20
US61/767,219 2013-02-20
PCT/US2014/017226 WO2014130589A1 (en) 2013-02-20 2014-02-19 Characterization of molecules in nanofluidics

Publications (1)

Publication Number Publication Date
CA2901460A1 true CA2901460A1 (en) 2014-08-28

Family

ID=51391777

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2901460A Pending CA2901460A1 (en) 2013-02-20 2014-02-19 Characterization of molecules in nanofluidics

Country Status (7)

Country Link
US (4) US9809855B2 (https=)
EP (1) EP2959015B1 (https=)
JP (2) JP6542676B2 (https=)
CN (1) CN105229168B (https=)
AU (1) AU2014219001A1 (https=)
CA (1) CA2901460A1 (https=)
WO (1) WO2014130589A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015130696A1 (en) 2014-02-25 2015-09-03 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
CN105229168B (zh) 2013-02-20 2020-07-17 生物纳米基因有限公司 纳米流体中分子的表征
US10844424B2 (en) 2013-02-20 2020-11-24 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
WO2018148903A1 (zh) * 2017-02-16 2018-08-23 上海亿康医学检验所有限公司 泌尿系统肿瘤的辅助诊断方法
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
JP2023554621A (ja) * 2020-12-15 2023-12-28 クアンタム-エスアイ インコーポレイテッド 超高感度バイオセンサ法
WO2024145268A2 (en) 2022-12-25 2024-07-04 Bionano Genomics, Inc. Optical genome mapping cartridges
EP4639178A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Optical genome mapping system
WO2024145270A1 (en) 2022-12-25 2024-07-04 Bionano Genomics, Inc. Instrumentation of optical genome mapping systems

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
WO2003010289A2 (en) * 2001-07-25 2003-02-06 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
CN101348763B (zh) * 2002-06-20 2012-07-18 普里梅拉蒂斯有限公司 用于多聚核苷酸检测和定量的设备
US8346482B2 (en) 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
US20100216153A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060063184A1 (en) 2004-09-09 2006-03-23 Felix Carolyn A Compositions and methods for the detection of DNA topoisomerase II complexes with DNA
AU2007226604A1 (en) * 2006-03-13 2007-09-20 Ikonisys, Inc. A non-invasive method for diagnosing fetal cells and cancer cells
EP3978121A1 (en) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
EP2604344A3 (en) * 2007-03-28 2014-07-16 BioNano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
PL2557520T3 (pl) * 2007-07-23 2021-10-11 The Chinese University Of Hong Kong Określanie zaburzenia równowagi sekwencji kwasu nukleinowego
EP2194847A1 (en) 2007-09-14 2010-06-16 Corventis, Inc. Adherent device with multiple physiological sensors
WO2009063778A1 (ja) 2007-11-12 2009-05-22 Murata Manufacturing Co., Ltd. 操作位置検出器およびその応用装置
CA2727095C (en) 2008-06-06 2020-01-07 Bionano Genomics, Inc. Integrated analysis devices and related fabrication methods and analysis techniques
US8628919B2 (en) * 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
CN108467887A (zh) 2008-11-18 2018-08-31 生物纳米基因公司 多核苷酸作图和测序
US20100137154A1 (en) * 2008-12-01 2010-06-03 Ach Robert A Genome analysis using a methyltransferase
KR20120039547A (ko) 2009-05-19 2012-04-25 바이오나노 제노믹스, 인크. 샘플 위치 및 배향의 동적 결정 및 동적 위치 전환을 위한 장치 및 방법
WO2011038327A1 (en) 2009-09-28 2011-03-31 Bionanomatrix, Inc. Nanochannel arrays and near-field illumination devices for polymer analysis and related methods
US20110117577A1 (en) 2009-10-20 2011-05-19 Agency For Science, Technology And Research Microfluidic system for trapping and detection of a biological entity in a sample
EP2491138A1 (en) 2009-10-21 2012-08-29 Bionano Genomics, Inc. Methods and related devices for single molecule whole genome analysis
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
SG183468A1 (en) * 2010-03-04 2012-09-27 Univ Singapore Microfluidics sorter for cell detection and isolation
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
IT1399366B1 (it) 2010-04-08 2013-04-16 Weightpack S P A Camma variabile, particolarmente per macchine per l'applicazione di tappi.
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
SG10201508373UA (en) 2010-10-20 2015-11-27 Bionano Genomics Inc Systems and methods for assessing biomolecule characteristics
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
WO2014014498A1 (en) 2012-07-20 2014-01-23 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
PL2561103T3 (pl) * 2011-06-29 2015-02-27 Bgi Diagnosis Co Ltd Nieinwazyjna detekcja anomalii genetycznych płodu
WO2013036860A1 (en) 2011-09-08 2013-03-14 Bionano Genomics, Inc. Physical map construction of whole genome and pooled clone mapping in nanochannel array
WO2013052907A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6073902B2 (ja) 2011-10-06 2017-02-01 セクエノム, インコーポレイテッド 遺伝的変異の非侵襲的評価のための方法およびプロセス
ES2624686T3 (es) 2011-10-11 2017-07-17 Sequenom, Inc. Métodos y procesos para la evaluación no invasiva de variaciones genéticas
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2861856C (en) 2012-01-20 2020-06-02 Sequenom, Inc. Diagnostic processes that factor experimental conditions
WO2013166517A1 (en) 2012-05-04 2013-11-07 Complete Genomics, Inc. Methods for determining absolute genome-wide copy number variations of complex tumors
EP3978621B1 (en) 2012-05-21 2023-08-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9347127B2 (en) 2012-07-16 2016-05-24 Veeco Instruments, Inc. Film deposition assisted by angular selective etch on a surface
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN105143462A (zh) 2013-02-05 2015-12-09 生物纳米基因有限公司 用于单分子分析的方法
CN105229168B (zh) 2013-02-20 2020-07-17 生物纳米基因有限公司 纳米流体中分子的表征

Also Published As

Publication number Publication date
US12275992B2 (en) 2025-04-15
US11359244B2 (en) 2022-06-14
JP2019205434A (ja) 2019-12-05
US10669586B2 (en) 2020-06-02
US20200362413A1 (en) 2020-11-19
US20220340973A1 (en) 2022-10-27
HK1219511A1 (zh) 2017-04-07
CN105229168A (zh) 2016-01-06
JP6542676B2 (ja) 2019-07-10
EP2959015A4 (en) 2016-08-17
EP2959015A1 (en) 2015-12-30
JP2016510590A (ja) 2016-04-11
AU2014219001A1 (en) 2015-10-08
US20180223361A1 (en) 2018-08-09
EP2959015B1 (en) 2020-11-04
JP6928032B2 (ja) 2021-09-01
WO2014130589A1 (en) 2014-08-28
CN105229168B (zh) 2020-07-17
US20160046992A1 (en) 2016-02-18
US9809855B2 (en) 2017-11-07

Similar Documents

Publication Publication Date Title
US12275992B2 (en) Characterization of molecules in nanofluidics
EP2462245B1 (en) Device and methods for epigenetic analysis
US11773429B2 (en) Reduction of bias in genomic coverage measurements
RU2753883C2 (ru) Набор зондов для анализа образцов днк и способы их использования
JP2005527220A (ja) 単一のポリマー分析を使用する方法および装置
US20150322515A1 (en) Methods and compositions for detecting target snp
US10844424B2 (en) Reduction of bias in genomic coverage measurements
Su et al. Single-molecule counting of point mutations by transient DNA binding
US20130337450A1 (en) Detection of quantitative genetic differences
Loh et al. Electric single-molecule hybridization detector for short DNA fragments
HK1219511B (zh) 纳米流体中分子的表徵
JP2007020526A (ja) 特異的結合を利用した標的分子の高感度検出法、そのキット
HK1231128A1 (en) Reduction of bias in genomic coverage measurements
HK1231128B (zh) 减小基因组覆盖测量中的偏差

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190219

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 11TH ANNIV.) - STANDARD

Year of fee payment: 11

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241211

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20241211

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20241211

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20250314

B12 Application deemed to be withdrawn, abandoned or lapsed

Free format text: ST27 STATUS EVENT CODE: N-2-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION

Effective date: 20250714

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 12TH ANNIV.) - STANDARD

Year of fee payment: 12

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20251209

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20251209