JP6501714B2 - 光学調査装置 - Google Patents
光学調査装置 Download PDFInfo
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- JP6501714B2 JP6501714B2 JP2015546140A JP2015546140A JP6501714B2 JP 6501714 B2 JP6501714 B2 JP 6501714B2 JP 2015546140 A JP2015546140 A JP 2015546140A JP 2015546140 A JP2015546140 A JP 2015546140A JP 6501714 B2 JP6501714 B2 JP 6501714B2
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Description
光学アセンブリは、励起光およびルミネセンス光の両方の空間プロファイルおよび/またはスペクトルプロファイルを定め、寄生光が検出器に到達することを防ぐための構成部品を含んでよい。例えば、対応する光路に沿って所与の光線のサイズを制限する空間フィルタがアセンブリ内のいくつかの箇所に提供されうる。励起光線のスペクトルコンテンツを排除するスペクトルプロファイルを有するスペクトルフィルタもまたルミネセンス光路に配置されうる。
一実施形態において、光学アセンブリは、励起光線およびルミネセンス光の少なくとも1つの空間プロファイルおよびスペクトルプロファイルの少なくとも1つを定めるための構成部品を含む。
本発明の典型的な実施形態は、光学調査装置に関する。
図1Aを参照すると、サンプル22内の蛍光体による蛍光発光を検出するための例示的な光学調査装置20の要素が図示的に示される。光モジュールは、様々な励起光路およびルミネセンス光路がサンプルの同じ側にあるエピ蛍光光学構成に基づく。少なくとも1つの共通光学部品が、サンプルに励起光を投射し蛍光発光を集光するために用いられる。図2〜5は、以下で更に詳述するように、それらの装置を具現化する光学アセンブリを示す。上述したように、サンプル22は、図6A、6B、および6Cに示すように、異なる励起スペクトルプロファイルおよび蛍光発光スペクトルプロファイルを有する複数の様々な蛍光体を含んでよい。
光学調査装置20は、第1に、複数の光源または光源アセンブリ24を含む。光源アセンブリは通常、光源と、光源に付随する追加部品とを含む。当業者は、選択された光源と併用するために追加部品が必要であるかを容易に判断することができるので、「光源」および「光源アセンブリ」という用語は本明細書において相互置換的に用いられる。
検出器アセンブリは通常、検出器と、検出器に付随する追加部品とを含む。当業者は、選択された検出器と併用するために追加の部品が必要であるかを容易に判断できるので、本明細書において「検出器」および「検出器アセンブリ」という用語は相互置換的に用いられる。
高感度、低ノイズ:低検出に関する要件は、一般的にpワットの集光された蛍光に制限し、PMTは固有利得(1M〜10M)および低ノイズを提供する。
光学調査装置20は更に、光源アセンブリ24、サンプル22、および検出器57の間で光を方向付ける光学アセンブリ25を含む。具体的には、光学アセンブリ25はまず、対応する光源アセンブリ24からサンプル22上の共通励起点23への、励起光線28の各々の様々な励起光路を定める。光学アセンブリ25は更に、サンプル22上の励起点23から検出器57へのルミネセンス光48のルミネセンス光路を定める。
図2〜5、7および7Aを参照すると、一例として、上述した光学調査装置20を具現化しうる光学アセンブリが示される。好適には、光学アセンブリは、製造を容易にし、光学部品の精密なアライメントの必要性を最低限にするか、またはなくすように設計される。
例1
上述したように、上述した例示的な実施形態に係る光学調査装置は、例えば図12に示すような試験装置を介してリアルタイムPCRシステムのために役立ちうる。
特徴(検出極限(LOD)および数値化極限(LOQ))となる蛍光形態は、青色、緑色、赤色、および近赤外(NIR)励起チャネルおよびペンタバンド検出フィルタに関して取得された。
この実験で用いられた光学設計の場合、色素の励起スペクトルが他の光学チャネルの励起帯域と重なり合うと相互干渉が生じ、1つの特定の色素の蛍光反応を分離することが困難になる。上に示す結果から、光学チャネル間の相互干渉の発生が見られる。相互干渉を数値化(および最終的に補償)するために、利用可能な励起波長ごとに各色素について校正曲線が得られた。
以下であった。
同じDFCD内の2つのターゲットの一般的な多重検出に関するプロトコルおよび結果がこの例で説明される。
計器によって作成されたデータは、以下に示すアルゴリズムを用いてオフラインで処理された。実行された50PCRサイクルの中で、PCR増幅の最初の45サイクルのみがデータ処理に必要であった。
データ分析の前にデータセットからバックグラウンドを除去するために線形回帰(傾斜およびy切片)が用いられた。このバックグラウンドは、例えば冷却された色素の残余蛍光、DFCD透過率および/または散乱における差分、DFCD材料または試薬によるバックグラウンド蛍光、バックグラウンドノイズ、標識プローブの変質などの複数の現象によって生じうる。
Claims (11)
- 各々が個々のスペクトルコンテンツを有する複数の励起光線によって励起されるサンプル内の被検体によって生じるルミネセンス光を検出するための調査装置であって、
前記被検体を励起するために前記サンプルに投射される前記複数の励起光線の1つを各々が発する複数の光源と、
前記サンプルによって生じる前記ルミネセンス光を検出するための少なくとも1つの検出器と、
光学アセンブリとを備え、
前記光学アセンブリは、前記サンプルに励起光を投射し前記サンプルからのルミネセンス光を集光する共通のサンプル側光学部品と、
前記共通のサンプル側光学部品から前記少なくとも1つの検出器への主軸を含み、前記複数の光源は前記主軸の周囲を取り巻くように配置され、前記光学アセンブリはさらに、
前記周囲を取り巻く光源から前記サンプル上の共通励起点へ前記励起光線の各々について異なる一定の励起光路と、
前記サンプル上の共通励起点から後方に伝播する前記少なくとも1つの検出器への前記ルミネセンス光の共有ルミネセンス光路と、
前記励起光線を前記主軸に近い前記共通のサンプル側光学部品内に前記共通励起点の方へ内側に方向換えするために前記光源からの前記励起光路に配置された内側方向換えアセンブリを有し、
前記励起光路および前記ルミネセンス光路は前記サンプルの同じ側に存在し、前記共通のサンプル側の光学部品はすべての前記励起光路内と前記ルミネセンス光路内に存在し、前記内側方向替えアセンブリは、前記励起光線を受け取り、前記共通のサンプル側光学部品の方へ前記励起光線を方向換えするために協働する外側反射部品と内側反射部品とを備える、調査装置。 - 前記光学アセンブリは更に、前記共有ルミネセンス光路に提供されたフィルタを備え、前記フィルタは、固定フィルタおよび作動式フィルタの1つであり、シングルバンドパスフィルタおよびマルチバンドパスフィルタの1つである、請求項1に記載の調査装置。
- 前記光学アセンブリは、単一の筐体内に含まれる、請求項1および2のいずれか1項に記載の調査装置。
- 前記光学アセンブリは、前記励起光線および前記ルミネセンス光の少なくとも1つの空間プロファイルおよびスペクトルプロファイルの少なくとも1つを定める構成部品を含む、請求項1〜3のいずれか1項に記載の調査装置。
- 前記構成部品は、対応する光路に沿って所与の光線のサイズを制限する空間フィルタである、請求項4に記載の調査装置。
- 前記光学アセンブリはさらに、スペクトルフィルタを含み、前記スペクトルフィルタは、前記励起光線のスペクトルコンテンツを排除するスペクトルプロファイルを有し、前記ルミネセンス光路に配置される、請求項5に記載の調査装置。
- 前記光学アセンブリは、検出のために前記フィルタされたルミネセンス光を出力する検出器側光学部品を含む、請求項1〜6のいずれか1項に記載の調査装置。
- 前記光学アセンブリは、前記サンプル側光学部品に向けて前記励起光線を導くための導波管を備える、請求項1〜7のいずれか1項に記載の調査装置。
- 前記サンプル内の被検体による前記ルミネセンス光は、少なくとも1つの光学現象の結果生じ、前記光学現象は、蛍光発光、燐光発光、生物発光、時間分解発光、および蛍光偏光の1つである、請求項1〜8のうちいずれか1項に記載の調査装置。
- 請求項1〜9のいずれか1項に記載の光学調査装置を含む、サンプルを光学検査するための試験装置。
- 前記試験装置は、ポリメラーゼ連鎖反応(PCR)、リアルタイムポリメラーゼ連鎖反応(rtPCR)、等温増幅再結合ポリメラーゼ増幅(RPA)の1つを実行するためのシステムによって具現化される、請求項10に記載の試験装置。
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