JP6449266B2 - 流体ピックアップを備えるマイクロ流体システム - Google Patents

流体ピックアップを備えるマイクロ流体システム Download PDF

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JP6449266B2
JP6449266B2 JP2016521890A JP2016521890A JP6449266B2 JP 6449266 B2 JP6449266 B2 JP 6449266B2 JP 2016521890 A JP2016521890 A JP 2016521890A JP 2016521890 A JP2016521890 A JP 2016521890A JP 6449266 B2 JP6449266 B2 JP 6449266B2
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sample
fluid
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JP2016528027A5 (https=
JP2016528027A (ja
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アンソニー・ジェイ・マカレヴィツ・ジュニア
リュック・ブッス
ステファノ・シアフィーノ
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
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    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50853—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates with covers or lids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Colloid Chemistry (AREA)
  • Accessories For Mixers (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micromachines (AREA)
  • Sampling And Sample Adjustment (AREA)
JP2016521890A 2013-06-21 2014-06-23 流体ピックアップを備えるマイクロ流体システム Active JP6449266B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361838063P 2013-06-21 2013-06-21
US61/838,063 2013-06-21
PCT/US2014/043715 WO2014205447A2 (en) 2013-06-21 2014-06-23 Microfluidic system with fluid pickups

Related Child Applications (1)

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JP2018228253A Division JP6790044B2 (ja) 2013-06-21 2018-12-05 流体ピックアップを備えるマイクロ流体システム

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JP2016528027A JP2016528027A (ja) 2016-09-15
JP2016528027A5 JP2016528027A5 (https=) 2017-07-27
JP6449266B2 true JP6449266B2 (ja) 2019-01-09

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JP2016521890A Active JP6449266B2 (ja) 2013-06-21 2014-06-23 流体ピックアップを備えるマイクロ流体システム
JP2018228253A Active JP6790044B2 (ja) 2013-06-21 2018-12-05 流体ピックアップを備えるマイクロ流体システム
JP2020184645A Active JP7110300B2 (ja) 2013-06-21 2020-11-04 流体ピックアップを備えるマイクロ流体システム

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JP2020184645A Active JP7110300B2 (ja) 2013-06-21 2020-11-04 流体ピックアップを備えるマイクロ流体システム

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Country Link
US (3) US9409174B2 (https=)
EP (1) EP3011302B1 (https=)
JP (3) JP6449266B2 (https=)
CN (2) CN105452839B (https=)
AU (1) AU2014284148B2 (https=)
SG (1) SG11201510439PA (https=)
WO (1) WO2014205447A2 (https=)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040002147A1 (en) 1999-12-29 2004-01-01 Desantis Grace Nitrilases
CA3081363A1 (en) 2001-02-21 2002-09-06 Basf Enzymes Llc Enzymes having alpha amylase activity and methods of use thereof
EP2319919B1 (en) 2001-06-21 2015-08-12 BASF Enzymes LLC Nitrilases
AU2003243157C1 (en) 2002-04-19 2008-09-04 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7547534B2 (en) 2002-06-14 2009-06-16 Verenium Corporation Methods for making a composition to treat a wood, a pulp or a paper
DK2194133T3 (en) 2003-03-06 2016-02-29 Basf Enzymes Llc Amylases, nucleic acids encoding them, and methods of making and using the same
US7592434B2 (en) 2003-04-04 2009-09-22 Verenium Corporation Pectate lyases, nucleic encoding them and methods for making and using them
CN103484486B (zh) 2003-07-02 2018-04-24 维莱尼姆公司 葡聚糖酶，编码它们的核酸以及制备和使用它们的方法
EP1668113B1 (en) 2003-08-11 2013-06-19 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
CN101432292B (zh) 2004-06-16 2013-03-13 维莱尼姆公司 对叶绿素进行酶促脱色的组合物和方法
EP2886658A1 (en) 2005-03-10 2015-06-24 BASF Enzymes LLC Lyase enzymes, nucleic acids encoding them and methods for making and using them
MX300732B (es) 2005-03-15 2012-06-28 Verenium Corp Celulasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
WO2007094852A2 (en) 2006-02-10 2007-08-23 Verenium Corporation Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP2548955A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
MX2008011477A (es) 2006-03-07 2008-09-23 Cargill Inc Aldolasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
BRPI0708614A2 (pt) 2006-03-07 2011-06-07 Verenium Corp aldolases, ácidos nucléicos codificando as mesmas e métodos para produzir e usar as mesmas
BRPI0714876B1 (pt) 2006-08-04 2022-04-19 Verenium Corporation Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos
AR062947A1 (es) 2006-09-21 2008-12-17 Verenium Corp Fosfolipasas acidos nucleicos que las codifican y metodos para prepararlas y usarlas
PL2069490T5 (pl) 2006-12-21 2018-09-28 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
NZ578309A (en) 2007-01-30 2012-06-29 Verenium Corp Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
EP2115153B1 (en) 2007-03-01 2013-06-05 BP Corporation North America Inc. Nitrilases, nucleic acids encoding them and methods for making and using them
WO2009045627A2 (en) 2007-10-03 2009-04-09 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
US9409174B2 (en) 2013-06-21 2016-08-09 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups
EP3708256B1 (en) 2015-04-22 2022-11-30 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
WO2016196210A2 (en) 2015-05-29 2016-12-08 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
WO2017078630A1 (en) * 2015-11-04 2017-05-11 Nitto Denko Corporation Apparatus and system for biofluid sample dispensing and/or assay
CN108700609A (zh) * 2015-12-30 2018-10-23 生物辐射实验室股份有限公司 具有自动校准的液滴测定系统
US10875026B2 (en) 2016-02-22 2020-12-29 Biofire Defense, Llc Devices and methods for rapid PCR
JP6622147B2 (ja) * 2016-06-16 2019-12-18 株式会社エンプラス エマルション生成部材、およびこれを用いたサンプル採取管、ならびに採血管
US11090652B2 (en) * 2016-07-28 2021-08-17 Biofire Defense, Llc Self-contained nucleic acid processing
USD803173S1 (en) * 2016-09-07 2017-11-21 EMULATE, Inc. Perfusion module lid without pressure features
GB201617869D0 (en) * 2016-10-21 2016-12-07 Blacktrace Holdings Limited A microfluidic device
CN108148743A (zh) * 2016-12-06 2018-06-12 中山百慧生物科技有限公司 一种液滴数字pcr芯片及相应检测方法和检测系统
CN108148744A (zh) * 2016-12-06 2018-06-12 中山百慧生物科技有限公司 一种液滴数字pcr芯片及相应检测方法和检测系统
CN110505918B (zh) * 2017-02-13 2022-06-14 生物辐射实验室股份有限公司 用于形成乳状液阵列的系统、方法以及装置
CN106984370B (zh) * 2017-05-08 2019-09-20 北京旌准医疗科技有限公司 一种基于微流控芯片的自动进样系统
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
GB2562762B (en) * 2017-05-24 2022-07-13 Univ Heriot Watt Microfluidic mixing
KR101951405B1 (ko) * 2017-06-28 2019-05-08 주식회사 파나진 시료 시약 반응 분석용 키트, 이를 이용한 표적 핵산 검출 장치 및 방법
EP3645167A4 (en) 2017-06-28 2021-02-24 Bio-rad Laboratories, Inc. SYSTEM AND METHOD FOR DROPLET DETECTION
US20190064173A1 (en) 2017-08-22 2019-02-28 10X Genomics, Inc. Methods of producing droplets including a particle and an analyte
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
US11045805B2 (en) 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
US11883825B2 (en) * 2017-11-02 2024-01-30 Memed Diagnostics Ltd. Cartridge and system for analyzing body liquid
JP6967427B2 (ja) * 2017-11-06 2021-11-17 Nok株式会社 ガスケットおよび密封構造
US11618023B2 (en) * 2017-12-06 2023-04-04 Samplix Aps Microfluidic device and a method for provision of emulsion droplets
KR101951670B1 (ko) 2017-12-13 2019-02-25 한국과학기술원 미세액적 내 유전자 검출을 위한 광학장치
EP3501651B1 (en) * 2017-12-22 2024-03-06 IMEC vzw Microfluidic routing
GB2572408B8 (en) * 2018-03-29 2022-06-22 Univ Heriot Watt Fluidic device
US20210031201A1 (en) * 2018-04-15 2021-02-04 Optofluidic Bioassay, Llc Differential pressure assisted drainage system
KR101891968B1 (ko) * 2018-05-30 2018-08-27 티엔에스(주) 하이브리드 유전자칩
KR101891558B1 (ko) * 2018-06-15 2018-08-27 티엔에스(주) 하이브리드 유전자칩용 유전자 분석 장치
CN113841053B (zh) * 2018-08-09 2024-05-28 港大科桥有限公司 用于将流体样本自动化处理成微流体液滴以用于体外诊断的系统
CN108728347A (zh) * 2018-08-27 2018-11-02 中国农业科学院农业质量标准与检测技术研究所 一种基因检测装置
CN109351368B (zh) * 2018-10-23 2021-04-30 深圳市博瑞生物科技有限公司 微流控芯片
CN109351369B (zh) * 2018-10-23 2021-01-26 深圳市博瑞生物科技有限公司 微流控液滴生成芯片
SG11202012943UA (en) * 2019-01-31 2021-01-28 Illumina Inc Thermoformed, injection molded, and/or overmolded microfluidic structures and techniques for making the same
EP3953044A4 (en) * 2019-04-08 2022-06-01 Technion Research & Development Foundation Limited MULTIPLEXED NETWORK OF NANOLITER DROPLET NETWORK DEVICES
WO2021020547A1 (ja) * 2019-08-01 2021-02-04 日本ケミファ株式会社 試薬カートリッジ
JP2023506664A (ja) * 2019-10-18 2023-02-17 モーニングバード メディア コーポレイション マルチヘッド押出機を備えた３次元プリンタ及びその内部で使用される組成物
CN111304060A (zh) * 2020-03-30 2020-06-19 西安天隆科技有限公司 一种微液滴生成芯片
CN115335141A (zh) * 2020-04-01 2022-11-11 默克专利股份有限公司 乳化装置
CN111826273B (zh) * 2020-07-22 2023-03-21 上海逢伙泰企业管理有限公司 一种用于核酸检测的自动化全封闭微流控芯片
WO2022060548A1 (en) * 2020-09-18 2022-03-24 Microgem International Plc Method and system for sample collection, storage, preparation and detection
DE102020211697A1 (de) * 2020-09-18 2022-03-24 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung
WO2023164124A1 (en) * 2022-02-25 2023-08-31 Redbud Labs, Inc. Sample tube connectors and methods for attaching a microfluidics device to a sample tube
CN114672412A (zh) * 2022-05-30 2022-06-28 季华实验室 一种微滴式数字聚合酶链反应芯片
EP4431185A1 (en) * 2023-03-17 2024-09-18 CSEM Centre Suisse d'Electronique et de Microtechnique SA - Recherche et Développement Liquid handling cover for a microfluidic device

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US20020092767A1 (en) * 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US6902703B2 (en) * 1999-05-03 2005-06-07 Ljl Biosystems, Inc. Integrated sample-processing system
US20030215957A1 (en) 1998-02-20 2003-11-20 Tony Lemmo Multi-channel dispensing system
WO2000049382A2 (en) * 1999-02-16 2000-08-24 The Perkin-Elmer Corporation Bead dispensing system
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
AU6932300A (en) 1999-08-25 2001-03-19 Caliper Technologies Corporation Dilutions in high throughput systems with a single vacuum source
NL1017374C2 (nl) * 2001-02-15 2002-08-16 Univ Delft Tech Inrichting voor het uitvoeren van een reactie, alsmede een werkwijze voor het uitvoeren van een reactie in de inrichting.
WO2002072423A1 (en) * 2001-03-09 2002-09-19 Biomicro Systems, Inc. Microplate lid
US20020187557A1 (en) * 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US6877528B2 (en) * 2002-04-17 2005-04-12 Cytonome, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
KR20050088476A (ko) 2002-12-30 2005-09-06 더 리전트 오브 더 유니버시티 오브 캘리포니아 병원균 검출과 분석을 위한 방법과 기구
EP1874457A1 (en) * 2005-04-26 2008-01-09 Aviza Technology, Ltd. Microfluidic structures and how to make them
US8257964B2 (en) * 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
WO2008143959A1 (en) * 2007-05-15 2008-11-27 Wako Pure Chemical Industries, Ltd. Pressure manifold to equalize pressure in integration pcr-ce microfluidic devices
ES2453107T3 (es) 2007-06-11 2014-04-04 Wako Pure Chemical Industries, Ltd. Procedimiento de amplificación de ácidos nucleicos usando una PCR sobre microchip con detección integrada de CE en tiempo real
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
CA2738578C (en) * 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US9157550B2 (en) * 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
NL2002862C2 (en) * 2009-05-08 2010-11-09 Friesland Brands Bv Microfluidic apparatus and method for generating a dispersion.
WO2011002957A2 (en) * 2009-07-01 2011-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
FR2951153A1 (fr) 2009-10-09 2011-04-15 Corning Inc Dispositif microfluidique
WO2012007787A1 (en) 2010-07-15 2012-01-19 Indian Statistical Institute Architectural layout for dilution with reduced wastage in digital microfluidic based lab-on-a-chip
DE102010047384B4 (de) * 2010-10-02 2012-06-28 Karlsruher Institut für Technologie Vorrichtung und Verfahren zur Erzeugung oder zur Ablage eines Fluidstroms aus Fluidsegmenten und ihre Verwendung
CN103429331B (zh) * 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
CN102389730A (zh) * 2011-08-23 2012-03-28 东南大学 一种双重乳液制备芯片
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US9409174B2 (en) 2013-06-21 2016-08-09 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups

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