JP6443811B2 - Ｍｒａｐ２ノックアウト - Google Patents

Ｍｒａｐ２ノックアウト Download PDF

Info

Publication number
JP6443811B2
JP6443811B2 JP2016525449A JP2016525449A JP6443811B2 JP 6443811 B2 JP6443811 B2 JP 6443811B2 JP 2016525449 A JP2016525449 A JP 2016525449A JP 2016525449 A JP2016525449 A JP 2016525449A JP 6443811 B2 JP6443811 B2 JP 6443811B2
Authority
JP
Japan
Prior art keywords
mrap2
mammal
cells
cell
mice
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016525449A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016523561A5 (enExample
JP2016523561A (ja
Inventor
ジョセフ エイ． マイズウブ
ジョセフ エイ． マイズウブ
浅井　真人
真人 浅井
Original Assignee
エフストック リミテッド ライアビリティ カンパニー
エフストック リミテッド ライアビリティ カンパニー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エフストック リミテッド ライアビリティ カンパニー, エフストック リミテッド ライアビリティ カンパニー filed Critical エフストック リミテッド ライアビリティ カンパニー
Publication of JP2016523561A publication Critical patent/JP2016523561A/ja
Publication of JP2016523561A5 publication Critical patent/JP2016523561A5/ja
Application granted granted Critical
Publication of JP6443811B2 publication Critical patent/JP6443811B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2016525449A 2013-07-10 2014-07-09 Ｍｒａｐ２ノックアウト Active JP6443811B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361844666P 2013-07-10 2013-07-10
US61/844,666 2013-07-10
PCT/US2014/045934 WO2015006437A1 (en) 2013-07-10 2014-07-09 Mrap2 knockouts

Publications (3)

Publication Number Publication Date
JP2016523561A JP2016523561A (ja) 2016-08-12
JP2016523561A5 JP2016523561A5 (enExample) 2017-08-10
JP6443811B2 true JP6443811B2 (ja) 2018-12-26

Family

ID=52280563

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016525449A Active JP6443811B2 (ja) 2013-07-10 2014-07-09 Ｍｒａｐ２ノックアウト

Country Status (8)

Country Link
US (2) US20160143256A1 (enExample)
EP (1) EP3019005B1 (enExample)
JP (1) JP6443811B2 (enExample)
KR (1) KR102297747B1 (enExample)
CN (1) CN105555132B (enExample)
AU (1) AU2014287250A1 (enExample)
CA (1) CA2917961A1 (enExample)
WO (1) WO2015006437A1 (enExample)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
CN107250148B (zh) 2014-12-03 2021-04-16 安捷伦科技有限公司 具有化学修饰的指导rna
ES2884838T3 (es) 2015-04-06 2021-12-13 Univ Leland Stanford Junior ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
RU2733841C2 (ru) * 2016-03-28 2020-10-07 Торэй Индастриз, Инк. Иммуноиндуцирующее средство
US20190112370A1 (en) * 2016-03-28 2019-04-18 Toray Industries, Inc. Pharmaceutical composition for treatment and/or prevention of cancers
KR101863653B1 (ko) * 2016-06-02 2018-06-05 전남대학교 산학협력단 락토페린 발현용 넉-인 벡터 및 이를 이용한 넉인 방법
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
CN115232812B (zh) * 2021-07-12 2025-10-24 南京启真基因工程有限公司 一种构建mrap2基因突变的重症早发性肥胖症模型猪核移植供体细胞的方法
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US6060642A (en) * 1997-08-15 2000-05-09 The Regents Of The University Of California Serotonin 5-HT6 receptor knockout mouse
EP1353549A2 (en) * 2000-06-01 2003-10-22 PHARMACIA & UPJOHN COMPANY Mice heterozygous for wfs1 gene as mouse models for depression
WO2005108415A2 (en) * 2004-04-30 2005-11-17 Biogen Idec Ma Inc. Membrane associated molecules
ES2626025T3 (es) 2005-10-18 2017-07-21 Precision Biosciences Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas
US20100120627A1 (en) * 2006-08-02 2010-05-13 Abdelmajid Belouchi Genemap of the human genes associated with psoriasis
CA3186126A1 (en) * 2011-09-21 2013-03-28 Sangamo Biosciences, Inc. Methods and compositions for regulation of transgene expression

Also Published As

Publication number Publication date
EP3019005B1 (en) 2019-02-20
CN105555132A (zh) 2016-05-04
KR20160042884A (ko) 2016-04-20
WO2015006437A1 (en) 2015-01-15
AU2014287250A1 (en) 2016-02-18
US20190313614A1 (en) 2019-10-17
JP2016523561A (ja) 2016-08-12
EP3019005A1 (en) 2016-05-18
KR102297747B1 (ko) 2021-09-06
EP3019005A4 (en) 2016-12-21
US20160143256A1 (en) 2016-05-26
CA2917961A1 (en) 2015-01-15
CN105555132B (zh) 2018-07-17

Similar Documents

Publication Publication Date Title
JP6443811B2 (ja) Ｍｒａｐ２ノックアウト
Bark et al. Developmentally regulated switch in alternatively spliced SNAP-25 isoforms alters facilitation of synaptic transmission
JP4368522B2 (ja) 哺乳動物におけるダブルマスル化を引き起こすミオスタチン遺伝子の変異
US11154041B2 (en) Genetically modified non-human animal with human or chimeric genes
Koerner et al. Toxicity of overexpressed MeCP2 is independent of HDAC3 activity
Aho et al. Periplakin gene targeting reveals a constituent of the cornified cell envelope dispensable for normal mouse development
Chae et al. Discrete change in volatile anesthetic sensitivity in mice with inactivated tandem pore potassium channel TRESK
JPWO2003004637A1 (ja) 体重または摂食量の調節に関与するヒスタミン受容体ｈ３遺伝子の利用
US20190029238A1 (en) Transgenic mice
JP2003513645A (ja) メラノコルチン−３レセプター欠失細胞、非ヒトトランスジェニック動物及び体重を調節する化合物の選択方法
Baur et al. Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts
CN112292030A (zh) 进行性骨化性纤维发育不良的啮齿类动物模型
AU770653B2 (en) Gene
US6080911A (en) Mice models of growth hormone insensitivity
US20220030837A1 (en) Novel Animal Model For Laing Distal Myopathy (Mpd1) And Methods of Use Thereof
US20220386574A1 (en) P21-cre animal models
RU2795136C2 (ru) Модель прогрессирующей оссифицирующей фибродисплазии на грызунах
JP2002543767A (ja) 生殖細胞と体細胞が４ｅ−ｂｐ１をコードするｄｎａにノックアウト突然変異を含有する非ヒトトランスジェニック動物
JP2002518056A (ja) アッセイにおけるＰｅｇ３遺伝子の使用、並びに肥満、体温調節および行動障害に関連する産物
US7445904B2 (en) Cysteine string protein and its role in neurodegenerative diseases
US20130291132A1 (en) Anf-rgc in-gene knock-out animal
Orquera et al. NKX2. 1 is critical for melanocortin neuron identity, hypothalamic Pomc expression and body weight
Grewe Characterization of the Von Willebrand Domain-Containing Protein 8 (Vwa8) Gene in Mouse
Schürmann Endogenous opioid peptides in drug addiction
US20090307790A1 (en) Isolation of the t-complex distorters and applications thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160401

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160204

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170627

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170627

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180524

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180713

TRDD Decision of grant or rejection written
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20181109

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20181112

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181116

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20181109

R150 Certificate of patent or registration of utility model

Ref document number: 6443811

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250