JP6424825B2 - 蛍光ナノ粒子標識体、多重免疫染色剤キットおよび多重免疫染色法 - Google Patents

蛍光ナノ粒子標識体、多重免疫染色剤キットおよび多重免疫染色法 Download PDF

Info

Publication number
JP6424825B2
JP6424825B2 JP2015539128A JP2015539128A JP6424825B2 JP 6424825 B2 JP6424825 B2 JP 6424825B2 JP 2015539128 A JP2015539128 A JP 2015539128A JP 2015539128 A JP2015539128 A JP 2015539128A JP 6424825 B2 JP6424825 B2 JP 6424825B2
Authority
JP
Japan
Prior art keywords
immunostaining
fluorescent
binding
biomolecule
phosphor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015539128A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2015045961A1 (ja
Inventor
拓司 相宮
拓司 相宮
健作 高梨
健作 高梨
古澤　直子
直子 古澤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Konica Minolta Inc
Original Assignee
Konica Minolta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Inc filed Critical Konica Minolta Inc
Publication of JPWO2015045961A1 publication Critical patent/JPWO2015045961A1/ja
Application granted granted Critical
Publication of JP6424825B2 publication Critical patent/JP6424825B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2015539128A 2013-09-26 2014-09-16 蛍光ナノ粒子標識体、多重免疫染色剤キットおよび多重免疫染色法 Active JP6424825B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013199781 2013-09-26
JP2013199781 2013-09-26
PCT/JP2014/074418 WO2015045961A1 (fr) 2013-09-26 2014-09-16 Marqueur à nanoparticules fluorescentes, kit d'immunocoloration multiple, et procédé d'immunocoloration multiple

Publications (2)

Publication Number Publication Date
JPWO2015045961A1 JPWO2015045961A1 (ja) 2017-03-09
JP6424825B2 true JP6424825B2 (ja) 2018-11-21

Family

ID=52743102

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015539128A Active JP6424825B2 (ja) 2013-09-26 2014-09-16 蛍光ナノ粒子標識体、多重免疫染色剤キットおよび多重免疫染色法

Country Status (2)

Country Link
JP (1) JP6424825B2 (fr)
WO (1) WO2015045961A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6834981B2 (ja) * 2015-12-18 2021-02-24 コニカミノルタ株式会社 蛍光物質集積ナノ粒子およびそれを用いた標識剤
JP7095603B2 (ja) * 2017-02-14 2022-07-05 コニカミノルタ株式会社 蛍光標識法
US11434376B2 (en) 2017-02-14 2022-09-06 Konica Minolta, Inc. Aminocoumarin compound, and aminocoumarin compound-containing resin particles
EP3608669A4 (fr) * 2017-04-07 2020-06-03 Konica Minolta, Inc. Particules fluorescentes pour prémélange, colorant fluorescent en contenant et procédé de marquage fluorescent les utilisant
JP7442069B2 (ja) * 2018-11-30 2024-03-04 パナソニックＩｐマネジメント株式会社 複合体及び検出装置
JPWO2021193682A1 (fr) * 2020-03-25 2021-09-30
WO2022059303A1 (fr) * 2020-09-17 2022-03-24 コニカミノルタ株式会社 Nanoparticules fluorescentes et procédé de préparation utilisant lesdites nanoparticules fluorescentes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3899407B2 (ja) * 2003-06-26 2007-03-28 国立大学法人 鹿児島大学 免疫組織化学的染色方法による抗原の検出方法
JP2009292804A (ja) * 2007-11-28 2009-12-17 Canon Inc リガンド分子固定ポリマー、リガンド分子固定粒子、標的物質の検出方法および標的物質の分離方法
JP5168092B2 (ja) * 2008-11-10 2013-03-21 コニカミノルタエムジー株式会社 半導体ナノ粒子標識剤
JP2012194013A (ja) * 2011-03-16 2012-10-11 Konica Minolta Medical & Graphic Inc 免疫組織化学染色方法及び反応試薬

Also Published As

Publication number Publication date
WO2015045961A1 (fr) 2015-04-02
JPWO2015045961A1 (ja) 2017-03-09

Similar Documents

Publication Publication Date Title
JP6424825B2 (ja) 蛍光ナノ粒子標識体、多重免疫染色剤キットおよび多重免疫染色法
Mukherjee et al. Quantum dot as probe for disease diagnosis and monitoring
JP6129330B2 (ja) 蛍光体集積ナノ粒子標識剤、およびこれを用いた蛍光免疫染色法
JP6127972B2 (ja) 組織染色方法
WO2015146896A1 (fr) Procédé d'analyse quantitative de matériau biologique sur la base d'une immunocoloration à antigènes multiples
JP6614161B2 (ja) 蛍光観察に使用する蛍光体集積ナノ粒子
JP6743703B2 (ja) 免疫染色法、およびこれに用いられる免疫染色試薬キット
JP6424826B2 (ja) 組織切片における生体物質の定量法
JP2012168165A (ja) 表面プラズモン励起増強蛍光分光法を利用する蛍光測定装置用センサチップを用いたアッセイ方法、およびアッセイ用キット
WO2019131727A1 (fr) Procédé pour évaluer un médicament
US20200165666A1 (en) Diluent for fluorescent nano particles, kit for immunofluorescent staining which utilizes same, solution for immunofluorescent staining, immunofluorescent staining method, and gene staining method
JP6443581B2 (ja) がんまたは免疫系が関係する疾患の診断または治療のための情報取得方法
JP2017227502A (ja) 組織切片から蛍光ナノ粒子の解離を防止する方法
WO2020148985A1 (fr) Nanoparticules intégrées au phosphore pour la détection d'une substance cible
JP7001083B2 (ja) 蛍光観察に使用する蛍光体集積ナノ粒子
WO2023058624A1 (fr) Procédé de coloration, procédé d'évaluation et échantillon
WO2020050373A1 (fr) Procédé d'acquisition d'informations, dispositif d'acquisition d'informations et programme
JP6398419B2 (ja) ソラフェニブを含有する標識剤
WO2022196203A1 (fr) Procédé de formation d'image

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170322

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180313

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180510

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180925

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181008

R150 Certificate of patent or registration of utility model

Ref document number: 6424825

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150