JP6415582B2 - タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物 - Google Patents

タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物 Download PDF

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JP6415582B2
JP6415582B2 JP2016550686A JP2016550686A JP6415582B2 JP 6415582 B2 JP6415582 B2 JP 6415582B2 JP 2016550686 A JP2016550686 A JP 2016550686A JP 2016550686 A JP2016550686 A JP 2016550686A JP 6415582 B2 JP6415582 B2 JP 6415582B2
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transduction reagent
protein transduction
protein
modified
cells
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JP2017503849A5 (enExample
JP2017503849A (ja
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ワン,ジィアンジュン
リー,キャンキャン
チョップ,マイケル
ジィアン,フェン
ウー,グオジュン
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ウェイン ステート ユニバーシティー
ウェイン ステート ユニバーシティー
カーゲン インク
カーゲン インク
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JP2016550686A 2013-10-25 2014-10-27 タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物 Active JP6415582B2 (ja)

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US201361895562P 2013-10-25 2013-10-25
US61/895,562 2013-10-25
PCT/US2014/062400 WO2015061779A1 (en) 2013-10-25 2014-10-27 Methods, systems and compositions relating to cell conversion via protein induced in-vivo cell reprogramming

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JP2018187189A Active JP6577112B2 (ja) 2013-10-25 2018-10-02 タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物
JP2019151759A Active JP6880125B2 (ja) 2013-10-25 2019-08-22 タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物

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JP2019151759A Active JP6880125B2 (ja) 2013-10-25 2019-08-22 タンパク質誘導生体内細胞リプログラミングによる細胞転換に関する方法、システム、及び組成物

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Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
JP6530206B2 (ja) * 2015-03-17 2019-06-12 学校法人同志社 上皮間葉転換阻害剤及び癌転移治療剤
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CN106110297B (zh) * 2016-07-05 2019-12-17 南通大学 Gfi1截短体的应用
CN110536685A (zh) * 2016-12-23 2019-12-03 昆士兰大学 Sox18蛋白活性的抑制剂用于治疗血管生成相关疾病和/或淋巴管生成相关疾病
JP2018132365A (ja) * 2017-02-14 2018-08-23 株式会社神戸製鋼所 マーキング装置
AU2018375192B2 (en) 2017-12-01 2023-11-09 Encoded Therapeutics, Inc. Engineered DNA binding proteins
US10785574B2 (en) 2017-12-14 2020-09-22 Flodesign Sonics, Inc. Acoustic transducer driver and controller
US11001618B2 (en) 2018-03-02 2021-05-11 Northwestern University Overexpression of FOXC1 to treat corneal vascularization
US11340226B2 (en) * 2018-03-02 2022-05-24 The Chinese University Of Hong Kong Squalene epoxidase in the diagnosis and treatment of non-alcoholic fatty liver diseases
JP7217542B2 (ja) * 2018-03-29 2023-02-03 国立大学法人 琉球大学 分化コントロール化合物を用いて造腫瘍性をもつおそれのある未分化iPS細胞等の混入を除去する方法
US12274733B2 (en) 2018-09-28 2025-04-15 President And Fellows Of Harvard College Cellular reprogramming to reverse aging and promote organ and tissue regeneration
AU2019347666B2 (en) 2018-09-28 2025-10-02 President And Fellows Of Harvard College Cellular reprogramming to reverse aging and promote organ and tissue regeneration
EP3942018A4 (en) * 2019-03-17 2022-12-07 Baylor College of Medicine Direct reprogramming of cardiac fibroblasts into cardiomyocytes using an endothelial cell transdifferentiation strategy
CN113710285A (zh) * 2019-03-26 2021-11-26 宾州研究基金会 用于治疗癌症的方法和材料
KR102150489B1 (ko) * 2019-04-09 2020-09-01 고려대학교 산학협력단 소변세포로부터 신장전구세포로의 직접 역분화를 유도하는 방법 및 이의 방법으로 역분화된 신장전구세포를 포함하는 신장세포 손상 질환 예방 또는 치료용 약학 조성물
US20210162002A1 (en) * 2019-10-17 2021-06-03 The Penn State Research Foundation Regenerating functional neurons for treatment of spinal cord injury and als
JP2022552002A (ja) * 2019-10-17 2022-12-14 ザ・ペン・ステイト・リサーチ・ファウンデイション 出血性脳卒中の治療のための機能的ニューロンの再生
CN114630699A (zh) * 2019-10-17 2022-06-14 宾州研究基金会 再生功能神经元以用于治疗神经病症
WO2021108609A1 (en) * 2019-11-25 2021-06-03 The Penn State Research Foundation Brain repair after traumatic brain injury through neurod1-mediated astrocyte-to-neuron conversion
CN112877364B (zh) * 2019-11-29 2023-07-28 中国医学科学院药物研究所 软骨下骨细胞向关节软骨细胞直接转化的重编程诱导方案
JPWO2021149823A1 (enExample) * 2020-01-24 2021-07-29
CN111214660B (zh) * 2020-02-25 2022-01-18 北京大学第三医院(北京大学第三临床医学院) Pax4基因表达抑制剂在制备抑制纤维化的药物中的应用
US11091524B1 (en) 2020-04-22 2021-08-17 Houston Gene Therapeutics Llc Compositions for treatment of vascular disease
CN114231489A (zh) * 2020-09-08 2022-03-25 纽伦捷生物医药科技(苏州)有限公司 用于重编程的功能性片段、组合及其应用
JP2023543360A (ja) * 2020-09-29 2023-10-13 ニューエクセル・セラピューティクス・インコーポレイテッド Dlx2ベクター
CA3197178A1 (en) * 2020-09-29 2022-04-07 NeuExcell Therapeutics Inc. Neurod1 combination vector
EP4221836A4 (en) * 2020-09-29 2025-02-19 Neuexcell Therapeutics Inc. NEUROD1 AND DLX2 VECTOR
JP2022104813A (ja) * 2020-12-29 2022-07-11 アイ ピース,インコーポレイテッド リプログラミング因子を導入された細胞の培養方法
CN113563428B (zh) * 2021-06-22 2022-08-02 呈诺再生医学科技(珠海横琴新区)有限公司 特定空间结构多肽及其在制备iPSC中的应用
CN117070477A (zh) * 2022-05-10 2023-11-17 纽伦捷生物医药科技(上海)有限公司 重组溶瘤病毒的重编程的功能性片段、组合及其应用
WO2024112118A1 (ko) * 2022-11-25 2024-05-30 고려대학교 산학협력단 방사선 치료에 의하여 손상된 근육 재생을 위한 고기능성 세포 치료제 조성물
US12252518B2 (en) 2023-01-06 2025-03-18 Life Biosciences, Inc. Methods of treating non-arteritic anterior ischemic optic neuropathy
WO2025106947A1 (en) * 2023-11-17 2025-05-22 Exir, Inc. Compositions and methods for induced stem cell differentiation to neuronal lineage

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
EP0671926B1 (en) 1992-08-11 2002-11-13 President And Fellows Of Harvard College Immunomodulatory peptides
FR2766195A1 (fr) 1997-07-21 1999-01-22 Transgene Sa Polymeres cationiques, complexes associant lesdits polymeres cationiques et des substances therapeutiquement actives comprenant au moins une charges negative, notamment des acides nucleiques, et leur utilisation en therapie genique
DE19839491A1 (de) 1998-08-29 2000-03-02 Hermann Heumann Verfahren zur Markierung von Biopolymeren mit Isotopen
US20040142474A1 (en) 2000-09-14 2004-07-22 Expression Genetics, Inc. Novel cationic lipopolymer as a biocompatible gene delivery agent
US6696038B1 (en) 2000-09-14 2004-02-24 Expression Genetics, Inc. Cationic lipopolymer as biocompatible gene delivery agent
DE60230147D1 (de) 2001-07-06 2009-01-15 Merck Patent Gmbh Verfahren zur überwachung und modulierung von proteinfaltung
WO2003046160A2 (en) 2001-11-30 2003-06-05 Applied Research Systems Ars Holding N.V. Methods of increasing protein expression levels
US20030134352A1 (en) 2002-01-04 2003-07-17 Freimuth Paul I. Facilitating protein folding and solubility by use of peptide extensions
CA2471178A1 (en) 2002-01-07 2003-07-17 European Molecular Biology Laboratory Recombinant protein expression
WO2005026196A2 (en) 2003-09-12 2005-03-24 Novexin Limited Methods for folding proteins and reducing protein aggregation
CA2548202C (en) 2003-11-25 2013-05-14 The Government Of The United States, As Represented By The Secretary Of The Army Use of shigella invaplex to transport functional proteins and transcriptionally active nucleic acids across mammalian cell membranes in vitro and in vivo
CN101528267A (zh) 2006-10-25 2009-09-09 皇家飞利浦电子股份有限公司 用于检测前列腺癌的造影剂
SG10202103401QA (en) 2008-03-17 2021-05-28 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
US8722348B2 (en) 2008-05-28 2014-05-13 Wayne State University Method and composition for a protein transduction technology and its applications
CN102272142A (zh) * 2008-12-23 2011-12-07 帷幄生物技术公司 非遗传修饰性重编程细胞的组合物和方法
EP2414510A4 (en) 2009-04-03 2013-04-17 Mclean Hospital Corp INDUCED PLURIPOTENTIAL STEM CELLS
US20120115225A1 (en) 2009-04-23 2012-05-10 Xu C W Reprogramming of somatic cells with purified proteins
EP2253700A1 (en) 2009-05-13 2010-11-24 Helmholtz-Zentrum für Infektionsforschung GmbH A method for producing test systems from donors suffering from adverse effects of medicaments and /or medical treatments, and uses of said systems
JP2013510563A (ja) 2009-11-13 2013-03-28 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 操作された微小胞を用いた真核細胞のリプログラミング
JP2011135864A (ja) * 2009-12-30 2011-07-14 Korea Univ Research & Business Foundation Oct4及びBmi1、またはその上位調節子を用いて体細胞から胚幹細胞類似細胞への逆分化を誘導する組成物及びこれを用いた胚幹細胞類似細胞の製造方法
JP2013518587A (ja) * 2010-02-04 2013-05-23 ヴィヴォスクリプト,インコーポレイテッド 神経学的障害を治療するために遺伝子改変を伴わずに細胞を再プログラミングするための組成物および方法
KR101788903B1 (ko) * 2010-08-19 2017-10-23 에프. 호프만-라 로슈 아게 체세포의 유도된 재프로그래밍된 신경 줄기 세포 (irnscs) 로의 전환
WO2012116064A1 (en) * 2011-02-22 2012-08-30 The Board Of Regents Of The University Of Texas Cardiac repair by reprogramming of cardiac fibroblasts into cardiomyocytes
WO2012151234A2 (en) 2011-05-02 2012-11-08 Wayne State University A protein-induced pluripotent cell technology uses thereof
US20140289882A1 (en) * 2011-07-19 2014-09-25 Oregon Health And Science University Compositions and methods for re-programming cells without genetic modification for repairing cartilage damage
JP6298405B2 (ja) 2011-08-30 2018-03-20 ザ ジェイ. デヴィッド グラッドストーン インスティテューツ 心筋細胞を生成するための方法
US20180223260A1 (en) * 2011-10-21 2018-08-09 Stemgenics, Inc. Functionalized nanoparticles for the intracellular delivery of biologically active molecules and methods for their manufacture and use

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